Electrochemical measurement of hydrogen peroxide in plasma: Evaluation of freshwater prawn (Macrobrachium rosenbergii) and crucian carp (Cyprinus carpio) phagocytes under natural conditions

An electrochemical technique for the real-time detection of hydrogen peroxide (H2O2) was employed to describe respiratory burst activity (RBA) of phagocytes in plasma which can be used to evaluate the ability of immune system and disease resistance. The method is based upon the electric current changes, by redox reaction on platinum electrode of extracellular hydrogen peroxide (H2O2) released from phagocytes stimulated by the zymosan at 680 mV direct current (d.c.). Compared with the control, activation of respiratory burst by zymosan particles results in a high amperometric response, and a current peak was obtained during the whole monitoring process. The peak current was proved by addition Of Cu2+ and other controls, to be the result of intense release of H2O2 from phagocytes. The peak area was calculated and used to evaluate the quantity of effective H2O2, which represents the quantity of H2O2 beyond the clearance of related enzymes in plasma. According to Faraday's law, the phagocytes' ability of prawns to generate effective H2O2 was evaluated from 1.253 x 10(-14) mol/cell to 6.146 x 10(-14) mol/cell, and carp from 1.689 x 10(-15) Mol/Cell to 7.873 x 10(-1)5 mol/cell. This method is an acute and quick detection of extracellular effective H2O2 in plasma and reflects the capacity of phagocytes under natural conditions, which could be applied for selecting species and parents with high immunity for breeding in aquaculture. (c) 2007 Elsevier Ltd. All rights reserved.

谷子遗传资源多样性研究进展

谷子形态水平，染色体水平，生化水平及DNA水平上遗传多样性的研究进展表明，谷子在不同生态环境下栽培，适应性结果导致了形态学性状和农艺性状的较大变异，染色体核型和带型的分析显示不同谷子品种在核型组成，核型分类方面存在分化；生化水平上，谷子的品质含量表现了丰富的多样性，蛋白质标记的指纹图谱也存在一定的差异，分子水平上，谷子种内存在一定的遗传多样性，分子标记是研究谷子遗传差异最有效方法，本文也对谷子育种如何有效利用遗传多样性研究的信息进行了探讨.

关于喀斯特石漠和石漠化概念的讨论

喀斯特石漠化是近年来所认识到的一种地质生态灾害，所造成的经济、环境乃至社会影响越来越大，受到国家的广泛关注，但却存在生态建设超前、基础研究落后的严峻现实。本文总结了当前石漠化的类型划分和存在问题，从喀斯特生态系统运行的地学过程、生物学过程和人为过程出发，提出石漠化过程存在地质石漠化过程、生态系统石漠化过程和人为加速石漠化过程，对不同类型的石漠化宜分别采取保护、恢复、重建、维持措施。石漠和石漠化表达的时空范畴不同，是石漠化这一土地退化过程的最后或顶极结果，自然条件下其形成多与地表坡度较大有关，而人为加速石漠化过程中石漠化土地空间分布与地形坡度、地貌部位并无直接联系。建议以“干扰方式+植被+土壤+地貌”对人为加速石漠化过程导致的石漠化土地进行类型划分，评价的土地应分为非石漠化土地、行漠化土地和逆转优化的“基准化”土地.

临沧锗矿床的稀土元素地球化学研究

通过对临沧锗矿床中基底花岗岩、矿化煤以及硅质岩的稀土元素地球化学研究认为盆地基底花岗提供了形成锗矿化的锗和形成硅质岩的硅；本区陆相热水成因硅质岩的Ce异常不明显，相对富集LREE；矿化煤相对富集HREE，这可能主要与矿化煤在含锗热液的相互作用过程中，煤对重烯土的优先富集有关。

个旧锰结核矿床地质地球化学与成因初步研究

个旧锰结核矿床产在锡石-多金属硫化物矿田顶部的第四系砂锡矿之中.从矿床地质产状、化学组成、微量元素、稀土元素、矿物组合等方面进行了讨论,认为个旧锰结核属于异地海相成因,后经搬运沉积在第四系砂锡矿之中;与中太平洋海山CAD19锰结核的物质组成和微量元素进行对比,表明个旧和CAD19锰结核有相类似的稀土模式,相对富集轻稀土,并出现明显的Eu负异常和Ce的正异常.