Differential expression of the Brunol/CELF family genes during Xenopus laevis early development

The BRUNOL/CELF family of RNA-binding proteins plays important roles in post-transcriptional regulation and has been implicated in several developmental processes. In this study, we describe the cloning and expression patterns of five Brunol genes in Xenopus laevis. Among them, only Brunol2 is maternally expressed and the zygotic expression of the other four Brunol genes starts at different developmental stages. During Xenopus development, Brunol1, 4-5 are exclusively expressed in the nervous system including domains in the brain, spinal cord, optic and otic vesicles. Brunol2 and 3 are expressed in both the somatic mesoderm and the nervous system. Brunol2 is also extensively expressed in the lens. In transfected Hela cells, BRUNOL1, 2 and 3 proteins are localized in both the cytoplasm and the nucleus, while BRUNOL4 and 5 are only present in the cytoplasm, indicating their different functions.

大熊猫线粒体DNA的九种限制酶图谱

用9种限制性内切酶分析大熊猫的线粒体DNA(mtDNA)。构建其中5种酶(BamHI 、EcoRI、EcoR V、Ps+I、PvuⅡ)的mtDNA物理图谱。大熊猫mtDNA的分子大小约 为16.4kb, 酶切位点是随机 分布的。图3表2参15

Pharmacokinetics of sifuvirtide, a novel anti-HIV-1 peptide, in monkeys and its inhibitory concentration in vitro

Aim: To study the pharmacokinetics of sifuvirtide, a novel anti-human immunodeficiency virus (HIV) peptide, in monkeys and to compare the inhibitory concentrations of sifuvirtide and enfuvirtide on HIV-1-infected-cell fusion. Methods: Monkeys received 1.2 mg/kg iv or sc of sifuvirtide. An on-line solid-phase extraction procedure combined with liquid chromatography tandem mass spectrometry (SPELC/MS/MS) was established and applied to determine the concentration of sifuvirtide in monkey plasma. A four-I-127 iodinated peptide was used as an internal standard. Fifty percent inhibitory concentration (IC50) of sifuvirtide on cell fusion was determined by co-cultivation assay. Results: The assay was validated with good precision and accuracy. The calibration curve for sifuvirtide in plasma was linear over a range of 4.88-5000 mu g/L, with correlation coefficients above 0.9923. After iv or sc administration, the observed peak concentrations of sifuvirtide were 10626 +/- 2886 mu g/L and 528 +/- 191 mu g/L, and the terminal elimination half-lives (T,12) were 6.3 +/- 0.9 h and 5.5 +/- 1.0 h, respectively. After sc, T-max was 0.25-2 h, and the absolute bioavailability was 49% +/- 13%. Sifuvirtide inhibited the syncytium formation between HIV-1 chronically infected cells and uninfected cells with an IC50 of 0.33 mu g/L. Conclusion: An on-line SPE-LC/MS/MS approach was established for peptide pharmacokinetic studies. Sifuvirtide was rapidly absorbed subcutaneously into the blood circulation. The T-1/2 of sifuvirtide was remarkably longer than that of its analog, enfuvirtide, reported in healthy monkeys and it conferred a long-term plasma concentration level which was higher than its IC50 in vitro.

芒果炭疽病[Colletotrichum gloeosporioides (Penz)]生物学及其综合防治研究

在云南景谷芒果种植区, 芒果炭疽病对芒果树嫩叶、嫩梢、花穗和果实为害率分别为85.7％、63.4％、77.1％和73.3％。风雨和昆虫媒介是传播的主要方式。发生为害最适温度为22℃--25℃, 相对湿度为85％--100％。该病在景谷种植区全年均可发生为害, 主要为害在发生期。发生前期为害, 是花穗干瘪和幼果掉落的原因之一。采取综合防治措施, 特别在发生前期适时防治, 可控制发生为害。

Neurophysiological recordings in freely moving monkeys

Recordings of neuronal activity in freely moving rats are common in experiments where electrical signals are transmitted using cables. Such techniques are not common in monkeys because their prehensile abilities are thought to preclude such techniques. Ho

糖在猕猴精子低温冷冻保存过程中的作用

TTE或TEST防冻液在冻存猕猴精子时产生不同结果，其主要不同在于防冻液中糖成分的不同。实验结果表明，冷冻复苏过程对精子结构产生了影响，TTE法低温保存的猕猴精子的头部的质膜出现少许皱褶或泡化现象，但精子的顶体、核或是精子尾部的结构与鲜精的结构基本相似。猕猴精子经TEST法低温保存后，大部分精子结构则发生了明显的变化，精子膜、顶体和精子核明显泡化、损伤或破裂，精子尾部不能分辨出正常的超微结构。这提示，可能由于TTE防冻液中复杂的糖成分在降温/复苏过程对精子起到了较好的协同冷冻保护作用；而TEST防冻液中单一的糖成分不能完全保护精子避免低温损伤，低温保存过程破坏了精子的结构，并影响了复苏后精子体外存活能力与受精能力。

滇中高原湖泊鱼类多样性的研究‘

依据历年馆藏的鱼类标本和资料，采用多元逐步回归与多项式逐步回归的分析方法，系统分析了滇中６个高原湖泊鱼类的物种丰度、特有种以及属的数目与７个主要湖泊环境因子之间的关系。分析结果显示，控制湖泊鱼类物种丰度、特有种数和属数的关键湖泊环境因子是湖泊面积和湖岸线长度；其中与湖泊面积呈显著正相关，而与湖岸线长度呈负相关。分析不停留于这一常规的横向比较，而是从历史生物地理学的角度，更深入一步揭示了滇中６个湖泊鱼类多样性的演化实质上是受湖泊的发育阶段所控制的。

广西高原鳅属鱼类一穴居新种记述

2003 年1 月, 在广西壮族自治区天峨县红水河水系地下河采集到一批盲鱼标本。经鉴定, 为高原鳅 属Triplophysa 一未经发表的新种。新种天峨高原鳅Triplophysa tianeensis sp1nov1 与个旧盲高原鳅T. gejiuensis 、 石林盲高原鳅T. shilinensis 、阿庐高原鳅T. aluensis 和南丹高原鳅T. nandanensis 相似; 本新种腹鳍末端不达 肛门, 尾鳍分枝鳍条16 , 可进一步与个旧盲高原鳅和石林盲高原鳅(腹鳍末端达到肛门, 尾鳍分枝鳍条14～ 15) 相区别; 本新种背鳍起点位于体之中点、腹鳍起点之后, 肛门紧靠臀鳍起点, 可进一步与阿庐高原鳅(背 鳍起点靠近吻端、位于腹鳍起点之前, 肛门距臀鳍起点仍有一段距离) 相区别。本新种与同分布于红水河水系 的南丹高原鳅Triplophysa nandanensis Lan et al1 较为相似; 但二者区别明显: 新种背鳍分枝鳍条7 、胸鳍分枝鳍 条9 、腹鳍分枝鳍条6 、背鳍外缘平截、背鳍起点位于腹鳍起点之后, 后者背鳍分枝鳍条8 、胸鳍分枝鳍条10～ 11 、腹鳍分枝鳍条7 、背鳍外缘凹入、背鳍起点位于腹鳍起点之前; 此外, 新种的穴居特征更为显著: 眼极度 退化、头长为眼径1618～3218 (2510) 倍、部分个体无色素斑且各鳍无斑点, 而南丹高原鳅眼小、头长为眼径 417～910 (715) 倍、体和头背侧密布云状斑且各鳍均具点状斑。

一篇探索真核细胞核起源的力作——读李靖炎先生的长篇近作“The primitive nucleus model and the origin of the cell nucleus”

《胞内共生与细胞研究》( 《Endocytobiosis and Cell Research》)是由国际胞内共生学会(International Society of Endocytobiology, ISE)主办的期刊, 它主要发表有关内共生物(endosy mbioses)和真核细胞的起源、发展、分化、进化和系统发育的研究论文. 在胞内共生和真核细胞的起源进化研究领域享有声誉. 去年该杂志发表了我国学者李靖炎先生的长篇论著“The primitive nucleus model and the origin of the cell nucleus(原始细胞核的模型与细胞核的起源)”(见1999, 13(1-3):1-86). 国外一家 SCI 收录的专业性刊物为中国学者发表一篇长达86页的 Review, 实不多见, 是我国学者在此领域的殊荣。