72 resultados para 12C6


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100MeV/u12C6+Saccharomyces cerevsiea YY,C03A,C03ApH:24%35pH5.010L,C03A,36h,12h;13.2%(V/V),1.6%(V/V)

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(HIRFL)12C6+(40,80120Gy,4Gy/min),M1,12C6+,,,,,"";:12C6+,,,

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30,90,180Gy312C6+,RAPDM1:12C6+,,M2M1,30GyC30Gy,90GyM1,M2RAPDDNA,;DNAM2

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p53(AdCMV-p53)p53AdCMV-p53/GFPHT-29PC-3AdCMV-p53/GFP0.5 Gy1.0 Gy2.0 Gy 12C6+/(H1299nullp53)(HepG2wtp53)(Helawtp53wtp53)(GFP)p53DAPIRT-PCRp53Western Blotp53MDM2p2112C6+ p5312C6+ AdCMV-GFPH1299HepG2Hela(p<0.05)AdCMV-GFP0.5 Gy 12C6+20 MOI AdCMV-p53H1299GFP50% (GFP90%)0.5 Gy1.0 Gy 12C6+40 MOI AdCMV-p53HepG2GFP44%(56.6%76.4%)0.5 Gy1.0 Gy 12C6+ 40 MOI AdCMV-p53HelaGFP37.8%50%(43.4%59.8%)12C6+ AdCMV-p53H1299HepG2Helap53(p<0.05)12C6+ AdCMV-p53p53p53(p<0.05)5AdCMV-p533p531312C6+ AdCMV-p533p53644%12C6+ AdCMV-p53H1299G0/G1G2/MG0/G1G2/M(p<0.05)AdCMV-p5312C6+ AdCMV-p53G0/G1636%G2/M1386%12C6+ AdCMV-p53HepG2G0/G1G0/G1(p<0.05)51.02.0 Gy 12C6+ AdCMV-p53G2/MAdCMV-p53G2/M(p<0.05)12C6+AdCMV-p53HelaG0/G1G2/M12C6+ AdCMV-p53G0/G1G2/M(p<0.05)12C6+ AdCMV-p53H1299HepG2Hela12C6+ AdCMV-p53(p<0.05)12C6+AdCMV-p5312C6+ AdCMV-p53H12998.066.0%9.363.5%12C6+AdCMV-p53HepG20.832.7%4.527.1% 12C6+AdCMV-p53Hela4.830.7%3.122.7%12C6+ AdCMV-p53(p<0.05)p53AdCMV-p53p53p53AdCMV-p53p53AdCMV-p53p53AdCMV-p53AdCMV-p53AdCMV-p53

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1. 2.B16 3. MLT 4. H22 1. 2.B164hB16Western-blotB16P53P21RT-PCRCHK2CDC25mRNA 3.1hDNA 4.H22 10.75GySODSODMDA0.3GyGSH0.5GyG2G1 2G2G1P53P21CHK2 mRNACDC25 mRNASODMDAB16 3DNASODMDA 41215Gy30% 1 12C6+G2G1 2 12C6+12C6+B16 3 15mg/kgMLT 4 12C6+H2215Gy

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X12C6+HL-60K562SMMC-7721HepG2(MTT)ATMSMC1-H2AXHL-60>K562>SMMC-7721>HepG2ATMATMATMATMATMHepG2HL-60,SMC1SSMC1S1h,HL-60HepG2H2AX,-H2AXHepG2HL-60-H2AX SMMC-7721M90%GO/G180%S60%G2/M>50%GO/G1SMMC-7721G2/M12C6+

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HIRFL-CSRm Monte-CarloB-MCarlsonHIRFL-CSRm - HIRFL-CSRm7.07MeV/u12C4+8.28MeV/u12C5+7.07MeV/u12C6+21.6MeV/u36Ar18+2.93MeV/u129Xe27+12C6+36Ar18+--36Ar18+Bump12C6+BumpBump12C6+36Ar18+HIRFL-CSR

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L02hprtDNAhprtDNAHIRFL12C6+(83.6MeV/nLET30keV/m)X8MVLET0.2keV/mL020~6Gy6-TG6-TGhprthprt9~10hprtDNAPCRhprt12C6+LET30 keV/mL02DNACASPDNAHDNADNATDNACLTLTMOliveOTM-SPSS11.5TM-L02hprt12C6+XL0212C6+X12C6+L02X1061GyXLETLETXL02TDNACLTLTMOTMTMLET12C6+DNALET(30keV/m)LEThprtXXhprtDNA

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[1]DNA[2,3] 1.XHL-7702AnnexinV-FITCX2.5Gy 2.XH2O21ul/ml15minH2O2 3.X1.5GyX1GyXX 1.XHL-7702X 2.XHL-7702H2O2 3.ROS 4.X12C6+

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nitric oxideNONO12C6+(X)SMMC-7721HepG2NO 1 X 2 NO 3 NO 4 NO

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HeLaReactive oxygen species, ROSNitric oxide, NOHeLaX12C6+ HeLa1%Dimethyl sulfoxideDMSOROSX1 GyX0.5%1%DMSOXDMSO1%DMSO0.5%DMSOConditioned mediumDMSODMSOWestern blottingDAF-FM DAInducible nitric oxide synthase, iNOSNODMSOXHeLaROSNODMSOBMS345541IKK/NF-BDMSOBMS345541NOROSNF-BNO

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(HIRFL)BJ116SrDNA 1.BJ1LETLETLET 2.BJ112C6+200-400GyLET60keV/m 3.12C15420 4.15415415470.34% 5.16SrDNABJ1154154 6.BJ1154 154 7.BJ11541542082.6%BJ1201541078.3%75.62%62% 8.BJ1154