Determination of arsenic species by capillary zone electrophoresis with large-volume field-amplified stacking injection

Determination of arsenic species by large-volume field amplified stacking injection-capillary zone electrophoresis (LV-FASI-CZE) is reported in this paper. Whole column injection was employed. The optimum buffer pH for the separation of weak acids was discussed. It was found that the optimum buffer to analyze the stacked arsenate (As(V)), monomethylarsonate (MMA), and dimethylarsinate (DMA) was 25 mm phosphate at pH 6.5. However, the optimum buffer to analyze the concentrated arsenite (As(III)) was 20 mm phosphate - 10 mm borate at pH 9.28. The limits of detection of the method developed were 0.026 mg/L for As(III), 0.023 mg/L for As(V), 0.043 mg/L for MMA, and 0.018 mg/L for DMA. An enrichment factor of 34-100 for several arsenic species was obtained. In the end, this method was applied to determine the arsenic concentration in the environmental reference materials to show the usefulness of the method developed.

Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater

A suitable method for the pretreutment of dissolved nitrate samples in seawaters for nitrogen isotopic analysis was established. First, the seawater samples were processed by removing nitrite and amonium. Then Devard's alloy was added in sample for conversion of dissolved nitrate to ammonium. The sample was distilled, and then the ammonium condensate was collected with zeolite. after distillation, the collected condensate was filtered and prepared for determining nitropic values. Some tests of the method were conducted. The distillation condition, the influence of salinity on nitrogen isotopic analysis, absorption of ammonium onto zeolite and an improved method on a large volume of seawater were discussed in this study. The results showed that the distillation step had an average recovery of (104.9 +/- 4.2) % (n = 6) when distillating every 300 mL aliquot of the sample under a strong alkaline condition with 0.5 g devard's alloy and a distillation time of 30 min. The nitrogen isotopic fractionation decreased markedly when salinity was increased from 0% to 0.5%; further increase(1% - 3.5%) showed little effect. The adsorption rate of ammonium onto zeolite had a high yield of (95.96 +/- 1.08) % (n = 6) in average. An improved collection method was used to process a large volume of seawater with several distillations, and had good effect on analysis. The method had been applied to analyze water samples collected from Changjiang estuary. The analytical results indicate that the method is suitable for delta N-15 analysis of dissolved nitrate in seawaters. The present method could provide valuable information about the source and cycle mechanism of dissolved nitrogen in estuary waters.

Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method

The Ludox-QPS method is a newly developed technique, which combines the Ludox HS 40 density centrifugation and quantitative protargol stain, to enumerate marine ciliates with good taxonomic resolution. We tested the method for simultaneous enumeration of diatoms, protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40. We conducted the evaluation with a sample size of 8.3 ml each from sandy, muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007. The Ludox-QPS method not only produced high extraction efficiencies of 97 +/- 1.3% for diatoms and 97.6 +/- 0.8% for ciliates, indicating a reliable enumeration for eukaryotic microbenthos, but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos, 97.9% for nematodes and 97.8% for copepods from sands, muddy sands and mud. By contrast, the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle, and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos: 89.4 +/- 2.0% from sands, 93 +/- 4.1% from muddy sands and 97.1 +/- 3.0% from mud. Apart from the sediment type, sample volume was another important factor affecting the extraction efficiency for meiobenthos. The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample. Besides the excellent extraction efficiency, the Ludox-QPS method obtained higher abundances of meiobenthos, in particular nematodes, than the routine Ludox centrifugation, which frequently resulted in an uncertain loss of small meiobenthos during the sieving process. Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation, showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom, protozoa and meiobenthos. Moreover, the comparatively high taxonomic resolution of the method, especially for diatoms and ciliates, makes it feasible to investigate microbial ecology at community level.

Computation of Viscous Incompressible Flow using Pressure Correction Method on Unstructured Chimera Grid

In this paper, a pressure correction algorithm for computing incompressible flows is modified and implemented on unstructured Chimera grid. Schwarz method is used to couple the solutions of different sub-domains. A new interpolation to ensure consistency between primary variables and auxiliary variables is proposed. Other important issues such as global mass conservation and order of accuracy in the interpolations are also discussed. Two numerical simulations are successfully performed. They include one steady case, the lid-driven cavity and one unsteady case, the flow around a circular cylinder. The results demonstrate a very good performance of the proposed scheme on unstructured Chimera grids. It prevents the decoupling of pressure field in the overlapping region and requires only little modification to the existing unstructured Navier–Stokes (NS) solver. The numerical experiments show the reliability and potential of this method in applying to practical problems.

A Continuation Method of Parameter Inversion for Non-Equilibrium Convection-Dispersion Equation

Based on the homotopy mapping, a globally convergent method of parameter inversion for non-equilibrium convection-dispersion equations (CDEs) is developed. Moreover, in order to further improve the computational efficiency of the algorithm, a properly smooth function, which is derived from the sigmoid function, is employed to update the homotopy parameter during iteration. Numerical results show the feature of global convergence and high performance of this method. In addition, even the measurement quantities are heavily contaminated by noises, and a good solution can be found.

Characters of Surface Deformation and Surface Wave in Thermal Capillary Convection

In the field of fluid mechanics, free surface phenomena is one of the most important physical processes. In the present research work, the surface deformation and surface wave caused by temperature difference of sidewalls in a rectangular cavity have been investigated. The horizontal cross-section of the container is 52 mmx42 mm, and there is a silicon oil layer of height 3.5 mm in the experimental cavity. Temperature difference between the two side walls of the cavity is increased gradually, and the flow on the liquid layer will develop from stable convection to un-stable convection. An optical diagnostic system consisting of a modified Michelson interferometer and image processor has been developed for study of the surface deformation and surface wave of thermal capillary convection. The Fourier transformation method is used to interferometer fringe analysis. The quantitative results of surface deformation and surface wave have been calculated from a serial of the interference fringe patterns.The characters of surface deformation and surface wave have been obtained. They are related with temperature gradient and surface tension. Surface deformation is fluctuant with time, which shows the character of surface wave. The cycle period of the wave is 4.8 s, and the amplitudes are from 0 to 0.55 mu m. The phase of the wave near the cool side of the cavity is opposite and correlative to that near the hot side. The present experiment proves that the surface wave of thermal capillary convection exists on liquid free surface, and it is wrapped in surface deformation.

An Extension of 2D Janbu’s Generalized Procedure of Slices for 3D Slope Stability Analysis II—Numerical Method and Applications

This paper provides a numerical approach on achieving the limit equilibrium method for 3D slope stability analysis proposed in the theoretical part of the previous paper. Some programming techniques are presented to ensure the maneuverability of the method. Three examples are introduced to illustrate the use of this method. The results are given in detail such as the local factor of safety and local potential sliding direction for a slope. As the method is an extension of 2D Janbu's generalized procedure of slices (GPS), the results obtained by GPS for the longitudinal sections of a slope are also given for comparison with the 3D results. A practical landslide in Yunyang, the Three Gorges, of China, is also analyzed by the present method. Moreover, the proposed method has the advantages and disadvantages of GPS. The problem frequently encountered in calculation process is still about the convergency, especially in analyzing the stability of a cutting corner. Some advice on discretization is given to ensure convergence when the present method is used. However, the problem about convergency still needs to be further explored based on the rigorous theoretical background.

Fast Computing For Lurr Of Earthquake Prediction

The LURR theory is a new approach for earthquake prediction, which achieves good results in earthquake prediction within the China mainland and regions in America, Japan and Australia. However, the expansion of the prediction region leads to the refinement of its longitude and latitude, and the increase of the time period. This requires increasingly more computations, and the volume of data reaches the order of GB, which will be very difficult for a single CPU. In this paper, a new method was introduced to solve this problem. Adopting the technology of domain decomposition and parallelizing using MPI, we developed a new parallel tempo-spatial scanning program.

Space experiments of thermocapillary convection in two-liquid layers

In 1999, the space experiments on the Marangoni convection and thermocapillary convection in a system of two immiscible liquid layers in microgravity environment were conducted on board the Chinese scientific satellite SJ-5. A new system of two-layer liquids such as FC-70 liquid and paraffin was used successfully, with the paraffin melted in the space. Two different test-cells are subjected to a temperature gradient perpendicular or parallel to the interface to study the Marangoni convection and thermocapillary convection, respectively. The experimental data obtained in the first Chinese space experiment of fluid are presented. Two-dimensional numerical simulations of thermocapillary convections are carried out using SIMPLEC method A reasonable agreement between the experimental investigation and the numerical results is obtained.

Numerical simulation of axisymmetric unsteady incompressible flow by a vorticity-velocity method

A new numerical method for solving the axisymmetric unsteady incompressible Navier-Stokes equations using vorticity-velocity variables and a staggered grid is presented. The solution is advanced in time with an explicit two-stage Runge-Kutta method. At each stage a vector Poisson equation for velocity is solved. Some important aspects of staggering of the variable location, divergence-free correction to the velocity held by means of a suitably chosen scalar potential and numerical treatment of the vorticity boundary condition are examined. The axisymmetric spherical Couette flow between two concentric differentially rotating spheres is computed as an initial value problem. Comparison of the computational results using a staggered grid with those using a non-staggered grid shows that the staggered grid is superior to the non-staggered grid. The computed scenario of the transition from zero-vortex to two-vortex flow at moderate Reynolds number agrees with that simulated using a pseudospectral method, thus validating the temporal accuracy of our method.

Influence of liquid bridge volume on the onset of oscillation in floating-zone convection .1. Experiments

The onset of oscillation in the floating zone convection driven by the gradient of surface tension was experimentally studied, and discussions were concentrated on the influence of liquid bridge volume on the onset of oscillation. Distributions of critical applied temperature difference and frequency depending on the volume of the liquid bridge were obtained, and there was a gap range of liquid volume which separated the curve of marginal stability into two parts for fixed rod diameter and aspect ratio. The results imply that the volume of the liquid bridge is a sensitive critical parameter for the onset of oscillation. The implication on the instability is also discussed in the present paper.

Influence of liquid bridge volume on the onset of oscillation in floating-zone convection .2. Numerical-simulation

The onset of oscillation in the floating zone convection driven by the gradient of surface tension was studied numerically for an unsteady and two-dimensional model, and studies were concentrated on the influence of liquid bridge volume on the onset of oscillation in comparison with the experimental results in the Paper I. The numerical results agree with the experimental ones presented in the previous paper, in which the distributions of critical applied temperature difference depending on the volume of liquid bridge and a gap range of liquid volume in marginal stability curve were obtained.

Domain decomposition hybrid method for numerical simulation of bluff body flows:theoretical model and application

The discrete vortex method is not capable of precisely predicting the bluff body flow separation and the fine structure of flow field in the vicinity of the body surface. In order to make a theoretical improvement over the method and to reduce the difficulty in finite-difference solution of N-S equations at high Reynolds number, in the present paper, we suggest a new numerical simulation model and a theoretical method for domain decomposition hybrid combination of finite-difference method and vortex method. Specifically, the full flow. field is decomposed into two domains. In the region of O(R) near the body surface (R is the characteristic dimension of body), we use the finite-difference method to solve the N-S equations and in the exterior domain, we take the Lagrange-Euler vortex method. The connection and coupling conditions for flow in the two domains are established. The specific numerical scheme of this theoretical model is given. As a preliminary application, some numerical simulations for flows at Re=100 and Re-1000 about a circular cylinder are made, and compared with the finite-difference solution of N-S equations for full flow field and experimental results, and the stability of the solution against the change of the interface between the two domains is examined. The results show that the method of the present paper has the advantage of finite-difference solution for N-S equations in precisely predicting the fine structure of flow field, as well as the advantage of vortex method in efficiently computing the global characteristics of the separated flow. It saves computer time and reduces the amount of computation, as compared with pure N-S equation solution. The present method can be used for numerical simulation of bluff body flow at high Reynolds number and would exhibit even greater merit in that case.

Compressible laminar boundary-layer flows of a dusty gas over a semi-infinite flat-plate

The compressible laminar boundary-layer flows of a dilute gas-particle mixture over a semi-infinite flat plate are investigated analytically. The governing equations are presented in a general form where more reasonable relations for the two-phase interaction and the gas viscosity are included. The detailed flow structures of the gas and particle phases are given in three distinct regions : the large-slip region near the leading edge, the moderate-slip region and the small-slip region far downstream. The asymptotic solutions for the two limiting regions are obtained by using a seriesexpansion method. The finite-difference solutions along the whole length of the plate are obtained by using implicit four-point and six-point schemes. The results from these two methods are compared and very good agreement is achieved. The characteristic quantities of the boundary layer are calculated and the effects on the flow produced by the particles are discussed. It is found that in the case of laminar boundary-layer flows, the skin friction and wall heat-transfer are higher and the displacement thickness is lower than in the pure-gas case alone. The results indicate that the Stokes-interaction relation is reasonable qualitatively but not correct quantitatively and a relevant non-Stokes relation of the interaction between the two phases should be specified when the particle Reynolds number is higher than unity.

利用Ghost Fluid方法模拟激波与柱形界面相互作用

利用Ghost Fluid方法(后面简称Ghost方法)和γ-model方法，在同样的时空离散精度条件下，对激波与柱形界面相互作用的二维可压缩流场进行了直接模拟，并与实验结果相比较．从模拟结果看，在短时间内， Ghost方法和γ-model方法模拟的结果与实验结果基本相同，两种方法均正确地模拟出界面的位置、激波的强度和速度．但随着时间的发展，具有较大数值耗散的γ-model方法的计算结果与实验差别越来越大；而数值耗散较小的Ghost方法能较为正确地模拟界面的运动．