Ordered Zinc Antimonate Nanoisland Attachment and Morphology Control of ZnO Nanobelts by Sb Doping

Hierarchical heterostructures of zinc antimonate nanoislands on ZnO nanobelts were prepared by simple annealing of the polymeric precursor. Sb can promote the growth of ZnO nanobelts along the [552] direction because of the segregation of Sb dopants on the +(001) and (110) surfaces of ZnO nanobelts. Furthermore, the ordered nanoislands of toothlike ZnSb2O6 along the [001](ZnO) direction and rodlike Zn7Sb2O12 along the [110](ZnO) direction can be formed because of the match relation of the lattice and polar charges between ZnO and zinc antimonate. The incorporation of Sb in a ZnO lattice induces composition fluctuation, and the growth of zinc antimonate nanoislands on nanobelt sides induces interface fluctuation, resulting in dominance of the bound exciton transition in the room temperature near-band-edge (NBE) emission at relatively low excitation intensity. At high excitation intensity, however, Auger recombination makes photogenerated electrons release phonon and relax from the conduction band to the trap states, causing the NBE emission to gradually saturate and redshift with increasing excitation intensity. The green emission more reasonably originates from the recombination of electrons in shallow traps with doubly charged V-O** oxygen vacancies. Because a V-O** center can trap a photoactivated electron and change to a singly charged oxygen vacancy V-O* state, its emission intensity exhibits a maximum with increasing excitation intensity.

Heteroepitaxial gold (111) rings on mica substrates

Two-dimensionally arranged gold rings were prepared by depositing a polymeric membrane bearing a dense array of uniform pores onto a mica substrate, filling the pores with a solution of a gold precursor, evaporation of the solvent and calcinations. The epitaxy of gold rings is confirmed by x-ray diffraction measurements, and the epitaxial relationship between gold rings and the mica was found to be Au(111)[1-10]parallel to mica(001)[010]. The polar and azimuthal angular spreads are 0.3 degrees and 1 degrees, respectively, which is at least equal to or better than the quality of the corresponding epitaxial gold-film on mica. (c) 2005 American Institute of Physics.

Determination of the absorption for incident light propagating parallel through the guest-host polymer film waveguide

Guest host polymer thin films of polymethyl methacrylate (PMMA) incorporated with (4'-nitrobenzene)-3-azo-9-ethylcarbazole (NAEC) were fabricated by spin coating and then poled by the method of corona-onset poling at elevated temperature. The absorption mechanism of the polymeric film, which is very important for the optical transmission losses and directly relates to the orientation of chromophore NAEC in polymer PMMA, was investigated in detail. From the UV-visible absorption spectra for NAEC/PMMA film before and after being poled, we determined the change of absorption coefficient kappa with the wavelength and approximately calculated the maximum absorption A(parallel tomax) as 3.46 for incident light propagating parallel through the film, i.e. the ordinary polarized light, which cannot be directly measured in the spectro photometer. (C) 2002 Elsevier Science Ltd. All rights reserved.

Polarization multiplexed write-once-read-many optical data storage in bacteriorhodopsin films

In polymeric films of bacteriorhodopsin (BR) a photoconversion product, which was named the F620 state, was observed on excitation of the film with 532 nm nanosecond laser pulses. This photoproduct shows a strong nonlinear absorption. Such BR films can be used for write-once-read-many (WORM) optical data storage. We demonstrate that a photoproduct similar or even identical to that obtained with nanosecond pulses is generated on excitation with 532 mn femtosecond pulses. This photoproduct also shows strong anisotropic absorption, which facilitates polarization storage of data. The product is thermally stable and is irretrievable to the initial B state either by photochemical reaction or through a thermal pathway. The experimental results indicate that the product is formed by a two-photon absorption process. Optical WORM storage is demonstrated by use of two polarization states, but more polarization states may be used. The combination of polarization data multiplexing and extremely short recording time in the femtosecond range enables very high data volumes to be stored within a very short time. (c) 2005 Optical Society of America.

聚合物超分子薄膜表面形貌及性质研究

与本体和溶液中的相行为相比，在薄膜状态下，分子链的运动是在其内在因素和外场的共同作用下进行的。微相分离的最终结构形态除了与超分子的组成，分子量，相分离强度有关外，还与薄膜厚度，表面和界面对组分的选择性亲和作用等因素有关。因此，所提出的问题就是如何利用这些影响薄膜结构的因素，构建具有新颖结构和有序结构的薄膜，以及如何实现薄膜形貌及结构尺寸的调控。另外一个重要方面就是如何利用聚合物超分子本身特殊性质，实现超分子薄膜表面结构随外界条件下的响应性变化。在薄膜的功能性方面，薄膜结构以及组成与薄膜性质有着怎样的关系，如何通过调节薄膜的结构以及组成，来优化薄膜的性质。 本论文利用高分子之间，以及高分子与无机金属离子之间的的非共价键相互作用（静电相互作用，氢键，络合作用）得到聚合物超分子体系。研究其自组装形成纳米结构的影响因素和机理，掌握调控纳米结构的形态、尺寸、以及薄膜光学性质的规律，实现通过调控薄膜微结构来优化薄膜性质的目的。 首先，本论文对聚合物超分子薄膜表面形貌的构建和调控方面进行了研究。利用两种嵌段共聚物之间的氢键作用，实现聚合物超分子络合体溶解性在共溶剂中的下降。在动力学控制的条件下，体系中未形成氢键的组分充当了“桥梁”作用，使形成的纳米球状聚集体发生在一维方向的聚集，得到柱状纳米聚集体。当两种聚合物之间全部形成氢键时，发生柱状纳米聚集体到球状胶束聚集体的转变。研究了溶剂挥发速度，聚合物溶液粘度等对形成纳米结构薄膜的影响。利用静电相互作用，得到聚合物超分子结构。实现了具有规则纳米孔洞结构的聚合物超分子薄膜的制备。研究了溶剂性质，溶剂挥发速度，聚合物溶液粘度，环境温度等对形成规则孔洞的影响，以及薄膜表面结构随外界条件的响应性变化， 并提出了孔洞形成及演变机理。 基于对薄膜表面形貌和结构的调控，我们对薄膜结构与薄膜的光学性质之间的关系进行了研究。利用两种均聚物之间的氢键相互作用，得到超分子嵌段共聚物。超分子嵌段共聚物发生微观相分离可得到自组装薄膜，利用氢键是一种弱的相互作用，使用选择性溶剂将某一组分除去，得到具有纳米孔洞的薄膜。薄膜表现出很好的抗反射性，在可见光区实现了98.00%的单波长高透过。在近红外区实现了宽波抗反射效果，透光率可达到99.00%以上，而且波段可调。深入研究了薄膜的厚度，刻蚀时间对薄膜透光率的影响。利用金属和聚合物之间的络合相互作用，得到被聚合物稳定的银纳米粒子。受聚合物与银纳米粒子之间的电子转移吸收的影响，复合纳米材料在薄膜状态下表现出很好的光致发光性质。研究了材料之间的配比，溶液的浓度，聚合物的分子量对形成的复合纳米粒子的尺寸及光致发光性能的影响。

高性能聚合物白光电致发光器件研究

聚合物白光电致发光器件可以采用旋涂、喷墨打印等方法成膜，具有工艺简单、成本低等优点，在全色平板显示和照明光源等方面具有广阔的应用前景，吸引了广泛的关注。聚合物白光电致发光器件同小分子发光器件相比发光效率仍然很低，如何提高聚合物白光器件的效率是急需解决的问题。而另一个评价白光器件性能的重要参数就是显色指数(color rendering index , CRI)。目前所报道的有机白光器件的显色指数普遍低于80，如何提高聚合物白光器件的显色指数也是我们研究的重点。本论文在载流子的注入平衡控制、白光聚合物薄膜的聚集态调控改善其光电性能和设计新型器件结构提高白光器件效率等方面进行了系统、深入的研究，在提高聚合物白光电致发光器件的效率和色纯度方面取得了较大的研究进展，为发展高质量的聚合物白光电致发光器件提供了理论基础和实施途径。 1. 首次将醇溶性含磷酸脂基团的聚芴衍生物作为电子注入材料应用到聚合物电致发光器件中，并采用高功函数铝作为阴极获得了高效率的聚合物电致发光。醇溶性含磷酸酯聚芴界面材料不但实现了多层聚合物结构，而且磷酸酯与金属铝的特殊作用有利于高效率电子注入。我们成功地将其应用到红、绿、蓝三基色聚合物发光器件中，与传统的Ca/Al电极聚合物器件相比，这种新型界面材料的使用有效降低了器件的工作电压，发光效率提高了25 %以上。 2. 利用物理掺杂的方法通过选用白光聚合物(由蓝光和橙光组成)作为主体、高效率红光磷光染料作为客体实现了包含红、绿、蓝三基色的高显色指数白光聚合物电致发光，色坐标为 (0.34，0.35)，显色指数达到 92。通过在发光层中引入电子传输材料实现了载流子的传输平衡，提高了白光器件的发光效率，发光效率达到5.3 cd/A。 3. 采用含苯并噻二唑基元的聚芴衍生物作为白光发光层(由蓝光和橙光组成)，利用热退火及溶剂处理等方式控制白光聚合物薄膜聚集态结构，有效调控了白光聚合物薄膜的载流子传输平衡和白光的色纯度。利用热处理或溶剂处理提高了薄膜的空穴迁移率，平衡了载流子传输，使发光效率由原来的7.3 cd/A提高到10 cd/A以上。同过热处理及溶剂处理后的白光聚合物薄膜中出现了高发光效率的聚芴结晶相，提高了蓝光部分的发光效率和色纯度，进而提高了白光的色纯度。 4. 利用含苯并噻二唑基元的聚芴衍生物白光聚合物和含磷酸酯醇溶性蓝光聚芴构筑了高效率双发光层聚合物白光电致发光器件。通过控制白光聚合物薄膜的聚集态结构提高空穴迁移率和引入醇溶性蓝光聚芴界面层改善电子注入实现了载流子传输平衡，同时将激子复合区域限制在两个发光层之间，避免了由于电极造成的激子淬灭，器件的发光效率达到17 cd/A和11 lm/W，是目前国际上报道的非掺杂型聚合物白光电致发光的最高值。 5. 利用高色纯度的蓝光聚芴、绿光和红光磷光染料实现了包含红绿蓝三基色的高显色指数白光；通过器件结构的设计提高器件中三线态激子的利用效率和抑制磷光染料中三线态激子向聚芴低三线态能级的能量转移，实现了高效率、高显色指数聚合物白光电致发光。 其中红光和绿光发光层由高三线态能级的聚乙烯咔唑掺杂绿光和红光磷光染料组成，蓝光发光层采用含磷酸脂基团聚芴，在两个发光层间引入高三线态能级激子限制层，有效抑制了磷光染料三线态激子被低三线态能级聚芴的淬灭，实现了高发光效率的包含红、绿、蓝三基色的高显色指数聚合物电致白光。器件最高效率可达到10.4 cd/A， 色坐标为 (0.36, 0.39)，显色指数达到91。

光导纤维pH传感器的光化学pH敏感高分子膜及pH响应性能

pH指示剂在高分子载体上的固定化研究是应光导纤维pH传感器的发展要求而发展起来的新的研究领域。pH指示剂在高分子固态载体上的固定直接决定着光导纤维pH传感器的灵敏度、响应时间、寿命等性能。迄今，吸附、包埋和化学键合三利，固定方法已得到广泛应用。吸附法与包埋法简单易行，但因指示剂易于逸出而无法保证试剂相的使用稳定性及寿命；化学键合法目前仅限于对含氨基等高反应活性基团的指示剂固定。另外，这三种固定技术均存在试剂相与分析对象呈现固／液两相分离的问题，它严重制约了传感器响应性能，成为pH指示剂固定技术发展的“瓶颈”。改善、发展与寻找新的指示剂／载体固定配对体系已成为本领域的研究焦点。本论文针对上述问题，开拓与发展了一系列指示剂固定于固态高分子载体上的新体系。（一）首先开拓与发展了酚红／交联聚乙烯醇与邻甲酚红／交联聚乙烯醇固定对的新体系。在酸性条件下，以甲醛做交联剂，通过在玻璃板上倾涂聚乙烯醇甲醛的凝胶溶液制备了高亲水性的交联聚乙烯醇载体膜；然后采用接枝共聚反应将丙烯酞胺单体与通过酸碱反应接枝有酚红或邻甲酚红的丙烯酞胺混合物接枝固定到交联聚乙烯醇薄膜上，首次将不含氨基的酚红、邻甲酚红在高亲水性的聚乙烯醇载体上固定。利用紫外可见光光谱仪测试了其响应性能。结果表明，固定化指示剂的光谱行为与其相应的水溶液的光谱行为不一致，这可能是固定化过程中新的化学键生成以及载体与指示剂之间的相互作用如氢键的作用等引起的。这也导致了其pH响应范围与相应指示剂水溶液的响应范围的差异。固定酚红交联聚乙烯醇pH敏感膜的pH响应范围为pH=6.72～8.49；固定邻甲酚红交联聚乙烯醇pH敏感膜的两个响应范围分别为［H~+］=0.1mol／L～5mol／L和pH7.97～12.26。这种方式制备的这两种pH敏感薄膜均表现出优异的可重复使用性、可逆性、稳定性及易于制备的特点。更突出的优点在于，与以聚丙烯酰胺为基质直接固定酚红的薄膜（PAM-PR）相比，这种固定技术制备的敏感膜，当其与分析剂溶液接触时，其接枝于表面的水溶性高分子溶解，呈现“液态”，在固态基质表面形成一“液膜”层，从而消除了试剂相与分析剂之间的相界面，克服了指示剂固定化技术中的“瓶颈”问题，大幅度地提高了其响应速度与灵敏度，使响应时间从PAM-PR的数十分钟降至30秒以下。（二）开拓了刚果红醋酸纤维素包埋对的pH敏感膜（CCM）及刚果红／环氧氯丙烷交联聚乙烯醇（PECM）和甲醛交联聚乙烯醇（PFCM）的三种pH敏感膜新体系，测试并比较了三种膜的响应性能。结果表明，固定化刚果红的光谱性质和响应范围与刚果红水溶液不一致，而且用不同载体固定的刚果红的光谱特征与响应范围也各不相同。CCM的响应范围为pH＝2.5～4.5，PECM的响应范围在［H~+］=2mol/L～pH＝6.8之间，PFCM响应范围为pH＝2.90～5.48。这也可能是固定化过程中的氢键效应、空间位阻、指示剂与载体间发生的化学反应不同及载体本身结构的差异等引起的。这三种膜也具有良好的重现性、可逆性及响应迅速（平均响应时间低于25秒）的特点。另外发现醋酸纤维素包埋刚果红的敏感膜具有特殊的稳定性。对这种特殊的稳定性原因的分析表明选择合适尺寸分子的指示剂与载体配对将可能克服包埋技术中指示剂逸出的缺点。（三）对制备的pH敏感膜及对应的水溶液的pH线性响应范围给出了相应的线性回归方程（R＞98.2）。结果表明这些敏感膜对响应范围内的pH均具有良好的线性响应关系。这些结果与思想不仅丰富了光导pH传感器的试剂相内容，也为后续工作提供了一些有益的借鉴。

pH值致变色高分子材料的合成与制备

本文研究和开拓了一类pH值致变色的高分子材料，它们的响应机理是分子链中有pH值致变色的基团，即PH值的变化使生色基团的结构发生变化，并最终导致其吸收光谱（吸收型）或发射光谱（荧光型）的变化。这类材料在如下领域，例如：光纤pH值传感膜材料，可重复使用的PH值指示材料，滤光材料，颜色材料，以及离子交换和离子选择性透过材料等领域有很好的应用前景。本文在总结前人研究的基础上，采用简单程序制备了多种响应快，长期稳定性好的光纤pH值传感膜材料，其可望用于制备光纤PH值传感器以满足不同领域的需求。工作大致包括以下五个方面。（一）采用一步法在碱性条件下让酚酞和邻甲酚酞分别与甲醛反应生成各自的高分子型pH值指示剂（PPF和cPF），GPc曲线表明它们的产物含有很多预聚物（如二聚体，三聚体和四聚体）和少量的高分子量组分。（二）分别制备了固定有PPF和CPF的两种聚合物膜（纤维素膜和聚乙烯醇膜）。沿用物理"包埋"的方法将PPF和CPF等低聚物固定在水解的二醋酸纤维素膜中，同时也开拓了共价键键接酚酞等到新的基质（PVA）上的方法，测试表明该类膜在pH8.0-14.0范围内具有很好的响应。（三）开拓了一种膜动力学分光光度法用于测定高碱度([OH-]=1-8SM），实验结果表明该方法可以提供一个快速（605）和准确（相对标准偏差为2．6％）的测定高碱度的方法。（四）制备了固定有酚红的PvA膜用于近中性pH值（6．5-10．5）和高酸度（5M[H+]-pH3．0）测定，该膜长期稳定性好，至少能连续使用3个月，特别适合生物学，海水，氨水溶液和高酸度的测量。（五）采用一步法在碱性条件下让荧光素与甲醛反应生成荧光齐聚物型pH值指示剂FLF，激光质谱表明它们的产物含有很多预聚物。可见光谱测试表明FLF-PVA膜的吸收光谱在pH0．0-10．0的范围内有很好的响应。

Non-modal instabilities of two-dimensional disturbances in plane Couette flow of a power-law fluid

Instabilities of fluid flows have traditionally been investigated by normal mode analysis, i.e. by linearizing the equations of flow and testing for unstable eigenvalues of the linearized problem. However, the results of eigenvalue analysis agree poorly in many cases with experiments, especially for shear flows. In this paper we study the instabilities of two-dimensional Couette flow of a polymeric fluid in the framework of non-modal stability theory rather than normal mode analysis. A power-law model is used to describe the polymeric liquid. We focus on the response to external excitations and initial conditions by examining the pseudospectra structures and the transient energy growths. For both Newtonian and non-Newtonian flows, the results show that there can be a rather large transient growth even though the linear operator of Couette flow has no unstable eigenvalue. The effects of non-Newtonian viscosity on the transient behaviors are examined in this study. The results show that the "shear-thinning/shear-thickening" effect increases/decreases the amplitude of responses to external excitations and initial conditions. (C) 2010 Elsevier B.V. All rights reserved.

中药蜘蛛香的化学成分研究及以羧甲基魔芋葡苷聚糖-壳聚糖为细胞膜的天冬酰胺酶人工细胞研究

本论文由三章组成。第一章介绍了中药蜘蛛香的化学成分的研究成果，第二章为羧甲基魔芋葡苷聚糖-壳聚糖为细胞膜的天冬酰胺酶人工细胞的研究，第三章综述了人工细胞在生物医学领域的应用。 第一章报道了中药蜘蛛香（Valeriana wallichii）根部乙醇提取物的化学成分，采用正、反相硅胶层析等分离方法和MS、NMR等多种波谱手段，从中共分离鉴定出17个化合物，分别为缬草素(valtrate，1)，valechlorine（2），homobadrinal（3），baldrinal（4），乙酰缬草素(acevaltrate 5)，valeriotetrate C（6），valeriotetrate B（7），对羟基苯乙酮(4'-hydroxy-acetophenone 8)，7-hydroxy valtrate（9），8-methylvalepotriate（10），1,5-dihydroxy-3,8-epoxyvalechlorine A（11），二氢缬草素(didrovaltrate 12)，胡萝卜苷（13），橙皮苷 (hesperidin 14)，prinsepiol-4-O-β-D-glucopyranoside（15），longiflorone（16），乙基糖苷（17）。其中化合物6、7、10、和11为新化合物，化合物9、15、16为首次从该植物中得到。新化合物11为含有氯原子的刚性骨架环烯醚萜，并且确定了其绝对构型。 第二章报道了以羧甲基魔芋葡苷聚糖（CKGM）和壳聚糖（CS）为膜的固定化L-天冬酰胺酶人工细胞研究成果。利用羧甲基魔芋葡苷聚糖和壳聚糖两种生物相容性很好的天然多糖之间的静电吸引力，在非常温和的条件下制备成具有半透过性膜的人工细胞，将治疗儿童急性成淋巴细胞性白血病(ALL)的药物L-天冬酰胺酶包裹在内。通过考察温度和pH对人工细胞的影响,结果表明以CKGM- CS为膜的L-天冬酰胺酶人工细胞对温度和pH的稳定性和耐受性均高于自由酶，说明CKGM-CS对酶具有保护作用，而且小分子底物和产物可以自由进出膜内外，而包裹在膜内的生物大分子则不能泄露出来。 第三章综述了微囊化人工细胞的研究进展。 This dissertation consists of three parts. In the first part, the chemical constituents from the root of Valeriana wallichii were reported. In the second part, preparation and characteristics of L-Asparaginase Artificial cell were reported. The third part is a review on progress of microcapsule artificial cell. The first chapter is about the isolation and identification of the chemical constituents from the root of V. wallichii. Seventeen compounds were isolated from the ethanol extract of roots of V. wallichii through repeated column chromatography on normal and reversed phase silica gel. By the spectroscopic and chemical evidence, their structures were elucidated as valtrate (1), valechlorine (2), homobadrinal (3), baldrinal (4), acevaltrate (5), valeriotetrate C (6), valeriotetrate B (7), 4'-hydroxy-acetophenone (8), 7-hydroxy valtrate (9), 8-methylvalepotriate (10), 1,5-dihydroxy-3,8-epoxyvalechlorine A (11), didrovaltrate (12), daucosterol (13), hesperidin (14), prinsepiol-4-O-β-D-glucopyranoside (15), longiflorone (16), and ethyl glucoside (17). Among them, 6, 7, 10, and 11 are new compounds. 15, 16 and 9 were isolated from this plant for the first time. The absolute configuration of compound 11, an unusual iridoid bearing a C-10 chlor-group and an oxo-bridge connecting C-3 and C-8 resulting in a rigid skeleton, was confirmed. The second chapter is about the semi-permeable microcapsule of carboxymethyl konjac glucomannan-chitosan for L-asparaginase immobilization. Carboxymethyl konjac glucomannan-chitosan (CKGM-CS) microcapsules, which have good biocompatibility, prepared under very mild conditions via polyelectrostatic complexation, were used for immobilize L-asparaginase-a kind of drug for acute lymphoblastic leukemia (ALL). The activity and stability under different temperature and pH of the enzyme loaded-microcapsules were studied. The results indicated the immobilized enzyme has better stability and activity contrasting to the native enzyme. The study illustrates that the L-asparaginase could be protected in CKGM-CS microcapsules, the substrate and product could pass through the system freely.

青藏高原栽培青稞遗传多样性研究

青稞，是我国藏区居民对裸大麦的称谓，它不仅是藏民的主要食粮、燃料和牲畜饲料，而且也是啤酒、医药和保健品生产的原料；青稞不仅为藏区人民的健康和经济发展做出了很大的贡献，而且对人类健康和社会经济的可持续发展都有重要的意义。青藏高原是我国及世界上青稞分布和种植面积最大的地区，资源极其丰富。虽然从经典遗传直到分子标记对我国大麦遗传多样性都有研究，但研究手段、数量仍然不够深入，对我国大麦资源遗传多样性研究的信息非常有限，不能很好地满足大麦遗传研究和育种应用的需要，尤其是对西藏栽培大麦的遗传多样性的研究还只是刚刚开始，关于栽培青稞多态性的研究报道很少。本研究采用SSR标记和蛋白质电泳两类技术，从SSR标记位点、单体醇溶蛋白、B组醇溶蛋白和淀粉粒结合蛋白（SGP）等四个方面对我国青藏高原栽培青稞的遗传多样性进行了综合评价。 SSR标记具有基因组分布广泛、数量丰富、多态性高、容易检测、共显性、结果稳定可靠、实验重现性好、操作简单、经济、易于高通量分析等许多优点，被认为是用于遗传多样性、品种鉴定、物种的系统发育、亲缘关系及起源等研究的非常有效的分子标记。本研究采用SSR标记分析了64份青藏高原栽培青稞的遗传多样性，同时评估SSR标记在我国大麦育种和品种鉴定中的应用潜力。选择了30个已知作图位点SSR标记，其中25个标记与重要性状的控制位点连锁紧密。选择的30个SSR标记，5个未得到很好的扩增产物，3个无多态性。22个多态性SSR标记位点中，每位点检测出等位基因2~15个，共检测出等位基因132个，平均每位点6.0 个。各多态位点检测出基因型为2~11种，位点HVM33的基因型最多。各多态位点的多态信息指数为0.16~0.91, 平均为0.65。根据PIC值选择了13个SSR标记用于我国青藏高原栽培青稞基因型鉴定，这些标记的PIC值为0.6以上。结合PIC值和基因型差异，选择了8个多态信息含量高的SSR标记，构建了高效指纹图谱，此图谱能把64份材料完全区分。 贮藏蛋白电泳分析是研究相关编码蛋白基因多态性的非常有效的方法。大麦单体蛋白与小麦醇溶蛋白相对应，具有丰富的多态性，可用于大麦遗传多样性、品种鉴定和群体进化等研究。本研究通过A-PAGE电泳技术研究了84份青藏高原栽培青稞的单体醇溶蛋白多态性。大麦单体醇溶蛋白图谱与小麦醇溶蛋白电泳图谱类似，所分离的蛋白清晰地分为ω－，γ－，β－和α－四个部分。青藏高原栽培青稞单体醇溶蛋白具有丰富的多态性，84份青稞材料中存在43条不同的蛋白带，75种组合带谱；其中67种为单一材料所独有，另8种则分别包含了2-3份材料。每份材料中拥有醇溶蛋白带为6－16条，含有6-10条单体醇溶蛋白带材料较多。西藏和四川材料群体单体醇溶蛋白多态性不同，具有区域特异性。西藏材料中发现了40条不同蛋白带，3条特异带，46 种蛋白组合；四川材料中出现了40种不同蛋白带，26种条带组合, 3条特异带。基于单体蛋白多态性的聚类与材料的来源有一定的相关性。A-PAGE单体蛋白具有丰富的多态性，可作为遗传研究和品种鉴定的标记。 大麦醇溶蛋白（hordein）是大麦籽粒的主要贮藏蛋白，与大麦的营养品质和加工品质密切相关，而且具有丰富的多态性，广泛用于品种鉴定、种质筛选、遗传多样性和亲缘关系研究。B组醇溶蛋白是主要的醇溶蛋白组份，约占总醇溶蛋白的80%，而且具有丰富的多态性。本研究采用SDS-PAGE分析了72份青藏高原栽培青稞B组醇溶蛋白的遗传多样性。青藏高原栽培青稞B组醇溶蛋白具有丰富的多态性，72份青稞材料中存在15种蛋白带，30种组合带谱，其中15种为单一材料所独有，另15种则分别包含了2-10份材料。每份材料中B组醇溶蛋白条带数为4-8条，含5、6条的材料较常见。不同来源的群体材料间B组醇溶蛋白组成存在差异，西藏青稞含有26种蛋白组合带谱，其中有19种特异带谱；四川群体中共发现11种蛋白组合带型，其中有4种特有带谱。两群体中都存在稀有条带。聚类分析将材料分成三组，材料聚类与材料来源地没有明显的相关性。 淀粉粒蛋白（Starch granule proteins, SGPs）是一类与淀粉粒结合的微量蛋白，一些淀粉粒蛋白具有淀粉生化合成中主要的酶蛋白功能，其变异会影响淀粉含量和特性，从而影响淀粉的应用。关于我国大麦淀粉粒组成研究还未见报道。本实验首次开创了我国大麦淀粉粒结合蛋白的研究工作。采用SDS-PAGE电泳技术研究了青藏高原栽培青稞的SGP组成，并分析了不同SGP组合间淀粉含量的差异，初步探索了所分离的SGP蛋白与淀粉合成的关系。66份青稞材料中分离了10种主要的SGP，其表观分子量为40-100KD，低于60KD的SGP带有7条，共有16种组合带谱；各SGP蛋白和组合带谱出现的频率存在差异，青藏高原青稞的SGP组成存在多态性。西藏青稞和四川青稞的SGP组成有很大差异，SGP组成具有地域差异性，西藏青稞含有12种蛋白组合带谱，其中有9种特异带谱；四川群体中共发现7种蛋白组合带型，其中有4种特有带谱；两群体中仅有3种共同的蛋白组合带谱。SGP蛋白特性将66份青稞分为三组, 即Ⅰ、Ⅱ、Ⅲ，材料聚类与材料来源具有一定的相关性。不同组合带谱材料间淀粉含量差异显著性检验结果显示，不同带谱间材料的总淀粉含量、直链淀粉含量和支链淀粉含量有差异，带谱2（SGP1+3+7+9+10）和8（SGP1+2+4+6+8）的总淀粉含量及支链淀粉含量显著大于组合带谱3（SGP1+3+7+10）的总淀粉含量。组合带谱7（SGP1+2+6+8）的直链淀粉含量显著低于带谱11（SGP1+5+8）的直链淀粉。带谱SGP2、3、4、5、6、7、8、9、10可能参与淀粉合成，SGP9可能与高支链淀粉的合成相关。 SSR标记位点、单体醇溶蛋白、B组醇溶蛋白、淀粉结合蛋白等四个方面的研究结果表明青藏高原SSR标记多态性、单体醇溶蛋白多态性、B组醇溶蛋白多态性和SGP多态性都非常丰富，与青藏高原是栽培青稞的多样性分布中心的观点一致。 青藏高原栽培青稞的SSR标记、单体醇溶蛋白、B组醇溶蛋白和SGP多态性表现出很大差异。SSR标记覆盖了整个基因组，多态性非常高。单体蛋白、B组醇溶蛋白、SGP蛋白是育种中非常关注的性状，他们只是代表基因组中的某一区域或位点，多态性相对较低。但单体蛋白多态性很高，84份材料中检测出43条不同蛋白带，75种不同的组合带谱。SSR标记技术和单体蛋白技术都是遗传多样性研究的有力工具，但单体蛋白技术不仅多态性高，而且经济、操作简便，是种质鉴定的理想方法。 对不同标记的多态性材料数据进行聚类，聚类图能为我们提供各材料间的遗传相似信息，为材料选择提供参考。但材料聚类与材料来源的地理区域的相关性表现不一致。SSR聚类和B组醇溶蛋白聚类与材料的来源地无相关性，而单体醇溶蛋白和SGP聚类与材料来源地有一定相关性，即西藏群体和四川群体分别有集中类群，这可能是人为选择的附加效应。 不同来源的群体材料的遗传多样性不同，具有区域特异稀有基因，加强不同地区间资源的交换和配合使用，有利于增加群体遗传多样性和新品种培育。 青藏高原栽培青稞的麦芽浸提性状、淀粉性状、病虫及裸粒等重要农艺性状控制位点存在丰富的变异，遗传基础宽广，可能蕴藏着多种不同的等位基因，是研究重要性状遗传特性、基因资源挖掘和遗传育种的宝贵资源库。 Hulless barley, due to its favorable attributes such as high feed value, good human nutrition，rich dietary fiber and ease processing, attracts people,s attention . Hulless barley plays a very important role in Tibetan life, used as essential food crop, main animal feed and important fuel. In addition to tsampa (roasted barley flour), a main food for Tibetan, hulless barley is also made into cake, soup, porridge, recent naked barley liquor and cornmeal. Qinghai-Tibet Plateau is one of a few areas which plant naked barley widely in the world and also has a long growing history. Genetic diversity of the cultivated hulless barley in this region , however, has not been documented. The study of genetic diversity existing within this population is of particular interest in germplasm identification, preservation, and new cultivar development. This study analyzed the genetic diversity of the cultivated naked barley from Qinghai-Tibet plateau through the study of SSR marker loci and monomeric prolamins, B-horden and starch granule proteins. SSRs are present abundantly in genomes of higher organisms and have become a popular marker system in plant studies. SSRs offer a number of advantages, such as the high level of polymorphisms, locus specificity, co-dominance, reproducibility, ease of use through PCRand random distribution throughout the genome. In barley, several hundred SSRs have been developed and genetically mapped and can therefore be selected from specific genomic regions. The genetic diversity of 64 cultivated naked barley from Tibet and Sichuan was studied with 30 SSRs of known map location.Among the selected SSR markers, PCR products of 5 SSR markers were not obtained and 3 SSR marker loci were monomeric. A total of 132 alleles were identified at 22 polyomeric SSR loci. The number of alleles per locus ranged from 2 to 15, with an average of 6.0. The polymorphism information content values for the SSRs ranged from 0.08 to 0.94, with an average of 0.65. 13 SSR markers with the PIC value >0.6 have been selected for discrimination of Qinghai-Tibet naked barley genotypews. A finger Print map was developed through 7 SSR markers with the high PIC value. It could be used as an efficient tool for gene discovery and identification of gernplasm. Hordeins, the main storage proteins of the barley seed, are composed of momomeric and polymeric prolamins and divided into -A, B, C and D groups in order of decreasing electrophoretic mobility. Hordeins show high inter-genotypic variation and have been extensively used as markers for cultivar identification and analyzing the genetic diversity. This study analyzed the genetic diversity of B-hordein in 72 naked barley from Qinqhai-Tibet Plateau. Extensive diversity was observed. A total of 15 different bands and 30 distinct patterns were found. Jaccard's coefficient of similarity was calculated, and the accessions were divided into three　main groups by cluster analysis using UPGMA. Differentiation among the populations from different collecting regions based on the polymorphism of B-hordein was investigated. Monomeric prolamins show high inter-genotypic variation and have been used as molecular markers for cultivar identification, analyzing the genetic diversity in collections and investigating the evolution processes and structure of populations However, the cultivated hulless accessions from Qinghai-Tibet Pateau in China have never been examined with respect to monomeric prolamins. This study analyzed the genetic diversity of monomeric prolamins (protein fraction corresponding to wheat gliadins) using the Acid -PAGE technique in eighty-four cultivated hulless barley from Qinqhai-Tibet Plateau in China. Extensive diversity was observed. A total of 43 different bands were found, of which 21 different bands were in the region of ω group, 8 in the region of γ, 8 in the region of β, and 6 in the region of α group. Among the 86 accessions, 75 distinct patterns were identified. The number of bands ranged from 6 to 16, depending on the variety. Jaccard’s coefficient of similarity was calculated, and the lines were grouped by cluster analysis using UPGMA. A dendrogram was obtained from the analysis of the groups and five main clusters were identified. No relationship between the distribution in the dendrogram and growth habits and origins of the cultivars could be detected. Starch is the major constituent of the cereal endosperm, comprising approximately 65% of the dry weight of the mature wheat grain. The starch formed in all organs of plants is packaged into starch granules, which vary widely between species and cultivars in size and shape. Wheat endosperm starch granules contain about corresponding to the main biosynthase of starch. This report firstly dealed with intraspecific variation of the major SGPs in cultivated naked barley from Qinghai-Tibet plateau. A total of 10 major SGPs were observed in the range of 40KD-100KD and 16 types of patterns were found. Based on the variation of SGPs, accessions studied were classified into 3 groups. A geographical cline of electrophoregram was observed. In addition, significance test of the difference of starch content among groups and types of patterns were done, and the results indicated those SGPs could be related to the content of starch. Diagram obtained through cluster analysis exhibited a structuration of diversity and genetic relationship among cultivated hulless accessions. In breeding program, parents with genetically distant relationship for hybridization will increase genetic diversity of progenies. In conclusion, cultivated naked barley from Qinghai-Tibet Plateau in China presents a high variability with respect to monomeric prolamins，SSR markers , B- hordeins and SGPs. The result of this study supports Qinghai-Tibet Plateau is the center of cultivated hulless barley and the cultivated naked barley is considered to be a gene pool with large diversity and could be applied to breeding for cereal.

Selective hydrogenation of cyclopentadiene in mono- and bimetallic catalytic hollow-fiber reactors

The mono- and bimetallic catalytic polymeric hollow-fiber reactors were established with catalytic polymeric cellulose acetate (CA) hollow fibers prepared by supporting the polymer-anchored mono- or bimetallic catalyst in/on the inner wall of the hollow fibers. The selective hydrogenation of cyclopentadiene to cyclopentene was efficiently carried out in the above catalytic polymeric hollow-fiber reactors, especially in the NaBH4 reduced bimetallic PVP-Pd-0.5Co/CA hollow-fiber reactor under mild conditions of 40 degrees C and 0.1 MPa. It was found that there was a remarkable synergic effect of palladium and cobalt reduced by NaBH4 in the bimetallic PVP-Pd-0.5Co/CA hollow-fiber reactor, which results in a 97.5% conversion of cyclopentadiene and a 98.4% selectivity for cyclopentene. (C) 2000 Elsevier Science B.V. All rights reserved.

Biodegradation of poly(L-lactide-co-glycolide) tube stents in bile

The objective of this study was to evaluate degradation behavior and the feasibility of biodegradable polymeric stents in common bile duct (CBD) repair and reconstruction. Various molar ratios of lactide (LA) and glycolide (GA) in poly(L-lactide-co-glycolide) (PLGA) were synthesized and processed into a circular tubing of similar to 10.0 mm outer diameter and a wall thickness of about 2.0 mm.

The Effect of MMT/Modified MMT on the Structure and Performance of the Superabsorbent Composite

A series of superabsorbent composites containing Montmorillonite (MMT), modified- Montmorillonite (OMMT) and sodium acrylate were synthesized by free-radical polymerization in aqueous solution. The structure of composites was characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and X-ray diffraction (XRD), and the results showed that the polymer chains were grafted onto the edge and the surface of MMT or OMMT. At the same time, the equilibrium swelling ratio of the composites was investigated as a function of the clay content and the results showed that the equilibrium swelling ratio of composites was improved by the introduction of clay.

Novel thin-film composite membranes with improved water flux from sulfonated cardo poly(arylene ether sulfone) bearing pendant amino groups

A new class of polymeric amine, namely, sulfonated cardo poly(arylene ether sulfone) (SPES-NH2) was synthesized and used for the preparation of thin-film composite membrane. The TFC membranes were prepared on a polysulfone supporting film through interfacial polymerization with trimesoyl chloride (TMC) solutions and amine solutions containing SPES-NH2 and m-phenylenediamine (MPDA). The resultant membranes were characterized with water permeation performance, chemical structure, hydrophilicity of active layer and membrane morphology including top surface and cross-section.