一种提取氧化葡萄糖酸杆菌山梨糖脱氢酶的简单方法

利用表面活性剂TritonX 10 0建立一种提取和测定氧化葡萄糖酸杆菌 (Gluconobacteroxydans )L 山梨糖脱氢酶 (L sorbosedehydrogenaseSDH)活性的简便方法 .最适提取条件为 :TritonX 10 0浓度 φ(Triton) / %=0 .3;提取温度θ=4℃ ;处理时间t/h =6～ 10h;用于提取的菌悬液D550nm范围 0 .0 90～ 0 .2 5 2 .该方法提取效率为细胞碎片法的 97.83%.图 2表 2参 7

船载综合信息显示系统半实物仿真设计与实现

针对船载综合信息显示系统在实体船舶内开发不方便的问题,提出了一种半实物仿真系统的设计与实现方法。结合"科学三号"海洋科学考察船的综合信息显示系统的设计和开发过程,从体系结构、信息流程和实现方法3个方面对半实物仿真系统的设计和实现过程进行了探索。实践结果表明,这种半实物仿真系统一方面可以为实际系统的体系结构、信息流程设计提供支持,另一方面可以为实际系统应用软件的开发提供较为真实的环境,有效的缩短开发时间和降低开发风险。

Fabrication and characterization of uniform Fe3O4 octahedral micro-crystals

Uniform Fe3O4 octahedral microcrystals with perfect appearance have been successfully synthesized by a Triton X100-assisted polyol process. During the polyols process for the preparation of Fe3O4 octahedra. the introduction of Triton X100 decreases significantly the needed concentration of NaOH. The results show that Fe3O4 octahedra are composed of eight triangular sheets, which are equilateral triangles. The edge size of Fe3O4 octahedron is about 4 mu m. The magnetic properties of Fe3O4 octahedral particles were evaluated on a SQUID magnetometer at room temperature.

Effect of Heat Treatment Temperature on the Catalytic Performances of PtRu/C Catalysts for Methanol Electro-Oxidation

Non-ionic surfactant Triton X-100 was used as a stabilizer to prepare PtRu/C catalysts for methanol oxidation reaction (MOR). The cyclic voltammogram was used to investigate the catalytic activity for MOR of different PtRu/C catalysts. TG-DTA, EDX, XRD, XPS and TEM were Used to characterize the composition, structure and morphology of the as-prepared PtRu/C catalysts. It is found that the heat treatment plays a crucial role in the particles size, particles distribution of the PtRu/C catalysts and the oxidation state of platinum. The results show that 350 degrees C is an optimum heat treatment temperature. The as-synthesized catalyst heat-treated at this temperature exhibits the best catalytic performance for MOR.

浊点萃取-石墨炉原子吸收光谱法测定环境样品中的镉

建立了浊点萃取分离富集石墨炉原子吸收光谱法测定环境样品中痕量镉的方法。浊点萃取选择8-羟基喹啉为螯合剂,Triton X-100为表面活性剂。在pH8～9、0.01%8-羟基喹啉和0.2%-Triton X-100、80℃水浴20 min的优化条件下,所建立方法的检出限为2.5 ng/L;加标回收率为94.6%～106.2%;对样品溶液进行富集的富集因子为17。利用该方法分别测定了2个实际水样和2个国家标准参考物质中的总镉含量,结果令人满意。

Daunomycin binding to detergent micelles: A model system for evaluating the hydrophobic contribution to drug-DNA interactions

The interaction of daunomycin with sodium dodecyl sulfate and Triton X-100 micelles was investigated as a model for the hydrophobic contribution to the free energy of DNA intercalation reactions. Measurements of visible absorbance, fluorescence lifetime, steady-state fluorescence emission intensity, and fluorescence anisotropy indicate that the anthraquinone ring partitions into the hydrophobic micelle interior. Fluorescence quenching experiments using both steady-state and lifetime measurements demonstrate reduced accessibility of daunomycin in sodium dodecyl sulfate micelles to the anionic quencher iodide and to the neutral quencher acrylamide. Quenching of daunomycin fluorescence by iodide in Triton X-100 micelles was similar to that seen with free daunomycin. Studies of the energetics of the interaction of daunomycin with micelles by fluorescence and absorbance titration methods and by isothermal titration calorimetry in the presence of excess micelles revealed that association with sodium dodecyl sulfate and Triton X-100 micelles is driven by a large negative enthalpy. Association of the drug with both types of micelles also has a favorable entropic contribution, which is larger in magnitude for Triton X-100 micelles than for sodium dodecyl sulfate micelles.

Hydrothermal growth and gas sensing property of flower-shaped SnS2 nanostructures

Unusual 3D flower-shaped SnS2 nanostructures have been synthesized using a mild hydrothermal treatment in the presence of octyl-phenol-ethoxylate ( Triton X-100) at 160 degrees C. The nanostructures have an average size of 1 mu m, and consist of interconnected nanosheets with thicknesses of about 40 nm. Based on time-dependent experimental results, we ascribe the oriented attachment mechanism to the growth of the SnS2 nanostructures. The nonionic surfactant Triton X-100 plays a key role in the formation of the flower-like morphology. Room temperature gas-sensing measurements show that the 3D SnS2 nanostructures could serve as sensor materials for the detection of NH3 molecules.

Effects of heteropoly acids and surfactant on electrochemiluminescence of tris(2,2 '-bipyridine) ruthenium(II)

The effects of heteropoly acids and Triton X-100 on electrochemiluminescence (ECL) of Ru(bpy)(3)(2+) are investigated. Triton X-100 prevents the oxidation of oxalate and results in an increase of the ECL signal. H5SiW11VO40 prevents the direct oxidation of oxalate and makes the electrochemical behavior of Ru(bpy)(3)(2+) less reversible, which leads to a decrease of the ECL signal. In contrast, H3PMo12O40 has negligible effect on ECL intensity. Some possible reasons for the effects on the ECL of Ru(bpy)(3)(2+) are discussed based on the adsorption of SiW11VO405- on electrode surface and the ion association between SiW11VO405- and Ru(bpy)(3)(2+). The signal of ECL decreases linearly with the concentration of heteropoly acid in the range from 2x10-6 to 1x10(-4) mol l(-1). The results indicate that ECL of RU(bpy)(3)(2+) is a potential sensitive and selective detection method for heteropoly acids and hence for the elements comprised in them.

铕-噻吩甲酰三氟丙酮-氯化甲基三烷基铵-异辛基苯氧基聚乙氧基乙醇体系荧光分光光度法测定痕量铕

本文首次提出了Eu~(3+)-TTA-N_(263)-异辛基苯氧基聚乙氧基乙醇(Triton X-100)体系的荧光分光光度法测定铕。在此最佳条件下铕的测定下限可达0.9ng/ml,并可允许存在一定量的其它共存稀土离子。该法用于测定包头混合稀土氧化物中的痕量铕,获得较满意的结果。

Isolation of PSI from a siphonous marine green alga, Codium fragile

Three different forms of PS I complexes were isolated from a siphonous marine green alga, Codium fragile, by Triton X-100 sucrose gradient centrifugation. Zone III had a Chl a/b>20, and designated as PS I. core complex CC I because it created only CP I band in mild PAGE. Zone IV and V had absorption at 436 and 674 nm, 467 and 650 nm, and 540 nm, suggesting the presence of Chl a, Chl b, siphonaxanthin and siphonein, Chl a/b were 3.23 and 2.4, respectively. Both CP I and CP I a bands were observed when they were subjected to mild PAGE. Therefore, Zone IV and V were different forms of PS I complexes that consisted of CC I and different amount of light-harvesting complex LHC I. Zone III contained only 66 and 56 ku peptides in SDS-PAGE, while Zone IV and V had 4 different LHC I peptides of 25, 26, 26.2 and 27.5 ku in addition to 66, 56 ku peptides. Fluorescence emission spectra showed that efficient energy transfer were kept among pigments in isolated PS I complexes. Excitation energy absorbed by Chl b, siphonaxanthin and siphonein can be transferred to Chl a.

Characteristics of nonlinear internal waves observed in the northern South China Sea

The South China Sea (SCS) is one of the most active areas of internal waves. We undertook a program of physical oceanography in the northern South China Sea from June to July of 2009, and conducted a 1-day observation from 15:40 of June 24 to 16:40 of June 25 using a chain of instruments, including temperature sensors, pressure sensors and temperature-pressure meters at a site (117.5A degrees E, 21A degrees N) northeast of the Dongsha Islands. We measured fluctuating tidal and subtidal properties with the thermistor-chain and a ship-mounted Acoustic Doppler Current Profiler, and observed a large-amplitude nonlinear internal wave passing the site followed by a number of small ones. To further investigate this phenomenon, we collected the tidal constituents from the TPXO7.1 dataset to evaluate the tidal characteristics at and around the recording site, from which we knew that the amplitude of the nonlinear internal wave was about 120 m and the period about 20 min. The horizontal and vertical velocities induced by the soliton were approximately 2 m/s and 0.5 m/s, respectively. This soliton occurred 2-3 days after a spring tide.

极化SAR图像海面船只监测方法研究

船只监测不论是在民用还是军用方面都有广阔的应用前景。极化合成孔径雷达（PolSAR）可以测量目标的极化散射特性，能够提高舰船检测的准确度和分类精度。论文以工程应用为背景，以深刻理解PolSAR图像成像原理及理论为基础，重点围绕PolSAR图像船海对比度增强和海上目标识别等方面开展了研究。 本文在文献综述的基础上，全面分析了单极化和PolSAR图像舰船目标检测的研究现状。在现有的PolSAR图像目标检测方法基础上，针对双极化数据首次提出二维卷积方法提高船海对比度；针对全极化数据改进广义最优化方法，构造极化特征向量，将回波强度和极化散射特征有机结合，提高船海对比度。上述两种方法能解决船只回波强度和海面回波强度相近或高海况下船目标检测问题。将聚类方法应用到海上目标识别中，采用非监督分类-模糊聚类可以将船只、海上人造目标、船尾迹等识别开来，在海上目标识别方面将有广阔的应用前景。 应用极化电磁波基本理论，将极化熵概念应用到船只监测。首次采用Bragg模式和双尺度模式分析两种不同极化熵随着入射角、波数和风场的变化情况，从而确定在低风速下Cloude熵相对Touzi熵更适合船只监测。

Postnatal development of the retina in root vole Microtus oeconomus

Vision plays an important role in the living habits of animals, especially in feeding. We investigated the postnatal development of retina in root vole Microtus oeconornus. The result shows that the retina of the M. oeconornus is very primitive before postnatal day （PD） 3. The neuroblastic layer does not differentiate and makes up more than half of the retina layer. The outer plexiform layer （OPL） first comes into existence at PDS. At PD6, as the presence of the OPL becomes obvious, the outer nuclear layer （ONL） and inner nuclear layer （INL） are much clearer. At PD18, the retina is similar to an adult retina and each layer becomes distinct. The thickness and cell density of the ganglion cell layer （GCL） and ONL during different postnatal days were also examined. These results show that the thickness and density of ONL increase during ontogeny, while the thickness and density of GCL decrease. Compared with Rattus norvegicus, Apodemus agrarius , Cricetulus triton, Microtus mandarinus , Myospalax cansus , Spermophilus dauricus and Sciurotamias davidianus, the histological structure of the retina of M. oeconornus is between that of nocturnal and diurnal rodents.

基于3自由度并联机器人的船载炮6维运动模拟系统

本文系统基于3自由度并联机器人和3维图形仿真实现了空间6维运动的模拟，构造了一种3自由度并联机构来模拟船的3维转动，并给出了并联平台的运动学逆解，采用图形仿真虚拟作战环境，模拟船的3维移动，并分析了图形驱动原因。

基于并联机器人的船载炮半物理仿真试验系统研究

20世纪90年代以来，并联机器人被广为注意，成了机器人技术新的热点。并联机器人技术被广泛地应用在工业、航天、航海等领域，尤其是并联机床，佩一出现就引起了广泛的关注，被称为是“21世纪的机床”，“20世纪机床首次革命性改型”。为了提高船载炮射击训练效益，解决船载炮兵训练的保障问题，同时也为船载炮兵训练的保障工作探索新的路子，提出研制基于并联机器人的船载炮半物理仿真试验系统，使训练可以不受天气、场地的限制。本系统采用模拟船、操瞄炮等设备与计算机图形虚拟技术相结合的方式组成船载炮半物理仿真试验系统。系统既要虚拟船载炮的作战环境，又要实现船在海浪中的6维运动。采用3自由度并联机器人平台构建模拟船，实现船的3维转动；采用计算机仿真虚拟作战环境，并实现船的3维移动。本文介绍了整个系统的软硬件构成，硬件从三自由度并联机构的运动学到控制系统的结构，软件从形成虚拟视景的图形驱动原理到主程序界面，最后展示了系统的硬件实物和软件界面的照片。基于并联机器人的船载炮半物理仿真试验系统具有以下特点：1．采用变频调速技术，研制了三自由度并联机构模拟系统，实现了船炮平台的运动模拟，使系统具有负载能力强、成本低、性能可靠等特点。2.整个系统由计算机图形分系统、动态数据采集与处理分系统、船的运动模拟分系统等组成，实现了船载炮兵射击指挥和火炮操作的模拟训练。 3．应用了多传感器信息融合技术，实现了多信息实时处理，达到了实时仿真的要求。系统能够实时检测船姿态和火炮操作状态等信息，评价射击效果。4.采用三自由度的旋转模拟与图像三自由度的平移运动相结合的方法，实现了六自由度的炮目相对运动模拟效果。该系统可用于模拟炮兵部队实施渡海登陆作战，对岸防目标、海上目标射击和指挥训练。完成船载炮兵的火炮操瞄和射击指挥的教学、训练及考核任务。