Molecular cloning and characterization of a thioester-containing protein from Zhikong scallop Chlamys farreri

Thioester-containing proteins are a family of proteins characterized by the unique intrachain beta-cysteinyl-gamma-glutamyl thioester, which play important roles in innate immune responses. The cDNA of Zhikong scallop Chlamys farreri thioester-containing protein (designated as CfTEP) was cloned by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of CfTEP was of 4616 bp, consisting of a 5 '-terminal untranslated region (UTR) of 30 bp and a 3 ' UTR of 140 bp with a polyadenylation signal sequence AATAAA and a poly(A) tail. The CfTEP cDNA encoded a polypeptide of 1481 amino acids with the theoretical isoelectric point of 5.98 and the predicted molecular weight of 161.4 kDa. The deduced amino acid sequence of CfTEP contained the canonical thioester motif GCGEQ, nine potential N-glycosylation sites and a C-terminal distinctive cysteine signature. It also contained a presumed catalytic histidine and proteolytic cleavage sites that were similar to C3 molecules. The high similarity of CfTEP with the thioester-containing proteins in other organisms, such as the TEPs from insects, the complement component C3, C4, C5 and the protease inhibitor alpha(2)-macroglobulin indicated that CfTEP should be a member of TEP family. The phylogenetic analysis revealed that CfTEP was closely related to TEPs from mollusc, nematodes and insects, and they formed a separate branch apart from the branches of complements factors and alpha(2)-macroglobulins. The spatial expression of CfTEP transcripts in healthy and bacterial challenged scallops was examined by semi-quantitative RT-PCR. The CfTEP transcripts were mainly detected in the tissues of hepatopancreas and gonad, and remarkably up-regulated by Microbial challenge, which suggested that CfTEP was a constitutive and inducible acute-phase protein involved in immune defense. These results provided new insights into the role of CfTEP in scallop immune responses, as well as the evolutionary origin of this important, widespread and functionally diversified family of proteins. (c) 2007 Published by Elsevier Ltd.

Generation and evolution of magma beneath the East Pacific Rise: Constraints from U-series disequilibrium and plagioclase-hosted melt inclusions

Mid-ocean ridge basalt (MORB) samples from the East Pacific Rise (EPR 12 degrees 50'N) were analyzed for U-series isotopes and compositions of plagioclase-hosted melt inclusions. The Ra-226 and Th-230 excesses are negatively correlated; the Ra-226 excess is positively correlated with Mg# and Sm/Nd, and is negatively correlated with La/Sm and Fe-8; the Th-230 excess is positively correlated with Fe-8 and La/Sm and is negatively correlated with Mg# and Sm/Nd. Interpretation of these correlations is critical for understanding the magmatic process. There are two models (the dynamic model and the "two-porosity" model) for interpreting these correlations, however, some crucial parameters used in these models are not ascertained. We propose instead a model to explain the U-series isotopic compositions based on the control of melt density variation. For melting either peridotite or the "marble-cake" mantle, the FeOt content, Th-230 excess and La/Sm ratio increases and Sm/Nd decreases with increasing pressure. A deep melt will evolve to a higher density and lower Mg# than a shallow melt, the former corresponds to a long residence time, which lowers the Ra-226 excess significantly. This model is supported by the existence of low Ra-226 excesses and high Th-230 excesses in MORBs having a high Fe-8 content and high density. The positive correlation of Ra-226 excess and magma liquidus temperature implies that the shallow melt is cooled less than the deep melt due to its low density and short residence time. The correlations among Fe-8, Ti-8 and Ca-8/Al-8 in plagioclase-hosted melt inclusions further prove that MORBs are formed from melts having a negative correlation in melting depths and degrees. The negative correlation of Ra-226 excess vs. chemical diversity index (standard deviation of Fe-8, Ti-8 and Ca-8/Al-8) of the melt inclusions is in accordance with the influence of a density-controlled magma residence time. We conclude that the magma density variation exerts significant control on residence time and U-series isotopic compositions. (c) 2010 Elsevier B.V. All rights reserved.

Living planktonic foraminifera from the northwestern Pacific Ocean: SSU rDNA sequences and their paleoceanographic significance

Living planktonic foraminifera (PF) samples from the Okinawa Trough of the northwestern Pacific Ocean were taken for DNA analysis. The SSU rDNA sequences of two PF species, Globigerina sp. and Pulleniatina obliquiloculata collected at Station WP01, were obtained and compared with those from the southwestern Pacific Ocean. Only small differences (< 0.7%-1.2% for P. obliquiloculata, and 0.3% for Globigerina sp.) were found between samples from the north- and south-western Pacific Ocean areas and this molecular evidence supported that these micropaleontological species are the same species, which implies that the West Pacific Ocean circulation system influences the planktonic foraminiferal gene communication.

节能型自治式多功能海洋环境监测系统的数据采集控制器的研究

在海洋学研究、海洋生产和工程建设应用中，经常需要对特定区域的海水参数进行长期、定点、实时的垂直剖面测量。锚系浮标和潜标是海洋监测网中最普遍采用的搭载平台之一，它们主要用于海洋环境定点监测。海洋浮标在研究海洋和大气的相互作用及全球气候变化、预报全球性和地区性海洋灾害、海洋污染监测、卫星遥感数据真实性校验、以及作为平台用于水声通讯和水下定位等方面发挥了重要作用，其缺点在于隐蔽性和安全性较差。潜标监测平台由于其主浮体安装在水下50-100米，具有良好的隐蔽性和安全性，在构建海洋立体监测系统中有较好的应用，但由于它总是隐藏在海表面以下，所搭载的测量传感器的种类有限，数据实时传送困难，从某种程度上也限制了其应用。 节能型自治式多功能海洋环境监测系统将定点锚泊潜标和浮标技术相结合，并创新性的融进了节能技术和自动防触碰功能，利用简约的传动储能节能技术，以C8051F340单片机为核心控制芯片，设计了低功耗、高可靠的数据采集控制存储系统的硬件电路和软件系统，使系统不但能够完成锚泊区域海表面水文参数的测量和数据传送，同时依靠单一的传感器组能够完成深达50米以上的水体次表层海洋要素的连续垂直剖面观测。系统利用SD卡实现了对海洋要素采集数据的实时存储和传送。低功耗、高可靠的数据采集控制存储系统的设计为海洋测量仪器实现智能化控制奠定了基础，提升了人类对海洋进行持续现场监测的能力。

敏捷制造:高技术产业化之路

“敏捷制造”是指企业能抓住瞬息即逝的机遇，快速推出高性能、高可靠性及顾客能接受的价格的新产品的能力。在敏捷制造企业中，有创新能力和经验的员工是企业的主要财富。敏捷制造为高新技术产业化开拓了生产规模与市场规模开拓同步增长的可行道路，又在企业与用户之间建立起崭新的“战略”依存关系。

表面相关多次波衰减方法与应用研究

The function of seismic data in prospecting and exploring oil and gas has exceeded ascertaining structural configuration early. In order to determine the advantageous target area more exactly, we need exactly image the subsurface media. So prestack migration imaging especially prestack depth migration has been used increasingly widely. Currently, seismic migration imaging methods are mainly based on primary energy and most of migration methods use one-way wave equation. Multiple will mask primary and sometimes will be regarded as primary and interferes with the imaging of primary, so multiple elimination is still a very important research subject. At present there are three different wavefield prediction and subtraction methods: wavefield extrapolation; feedback loop; and inverse-scattering series. I mainly do research on feedback loop method in this paper. Feedback loop method includs prediction and subtraction.Currently this method has some problems as follows. Firstly, feedback loop method requires the seismic data used to predict multiple is full wavefield data, but usually the original seismic data don’t meet this assumption, so seismic data must be regularized. Secondly, Multiple predicted through feedback loop method usually can’t match the real multiple in seismic data and they are different in amplitude, phase and arrrival time. So we need match the predicted multiple and that in seismic data through estimating filtering factors and subtract multiple from seismic data. It is the key for multiple elimination how to select a correct matching filtering method. There are many matching filtering methods and I put emphasis on Least-square adaptive matching filtering and L1-norm minimizing adaptive matching filtering methods. Least-square adaptive matching filtering method is computationally very fast, but it has two assumptions: the signal has minimum energy and is orthogonal to the noise. When seismic data don’t meet the two assumptions, this method can’t get good matching results and then can’t attenuate multiple correctly. L1-norm adaptive matching filtering methods can avoid these two assumptions and then get good matching results, but this method is computationally a little slow. The results of my research are as follows: 1. Proposed a method that interpolates seismic traces based on F-K migration and demigration. The main advantage of this method is that it can interpolate seismic traces in any offsets. It shows this method is valid through a simple model. 2. Comparing different Least-square adaptive matching filtering methods. The results show that equipose multi-channel adaptive matching filtering methods can get better results of multiple elimination than other matcing methods through three model data and two field data. 3. Proposed equipose multi-channel L1-norm adaptive matching filtering method. Because L1-norm is robust to large amplitude differences, there are no assumption on the signal has minimum energy and orthogonality, this method can get better results of multiple elimination. 4. Research on multiple elimination in inverse data space. The method is a new multiple elimination method and it is different from those methods mentioned above.The advantages of this method is that it is simple in theory and no need for the adaptive subtraction and computationally very fast. The disadvantage of this method is that it is not stabilized in its solution. The results show that equipose multi-channel and equipose pesudo-multi-channel least-square matching filtering and equipose multi-channel and equipose pesudo-multi-channel L1-norm matching filtering methods can get better results of multiple elimination than other matcing methods through three model data and many field data.

乌鲁木齐河源1号冰川表层雪的化学特征——以低分子有机酸和无机阴离子为例

表层雪是联系大气成分与冰芯记录的重要纽带,是研究成冰作用过程中化学组成变化的起点.为配合天山乌鲁木齐河源1号冰川成冰作用过程中化学组成变化的研究,对1号冰川积累区(海拔4130 m)一个完整年度的表层雪样品进行了低分子有机酸和无机阴离子含量的分析.结果显示:表层雪中低分子有机酸主要有HCOO-、CH3COO-、C2H5COO-和(COO)22-,无机阴离子主要有F-、Cl-、NO2-、NO3-、SO42-和PO43-.除(COO)22-外,大部分高浓度的有机酸…