Simulation of Natural Gas Production in Hydrate Reservoirs

This paper simulates a one-dimensional physical model of natural gas production from hydrate dissociation in a reservoir by depressurization. According to the principles of solid hydrate decomposition in stratum and flow of natural gas in porous medium, the pressure governing equations for both gas zone and hydrate zone are set up based on the physical production model. Using the approximation reported by N. N. Verigin et al. (1980), the nonlinear governing equations are simplified and the self-similar solutions are obtained. Through calculation, for different reservoir parameters, the distribution characters of pressure are analyzed. The decline trend of natural gas production rate with time is also studied. The simulation results show that production of natural gas from a hydrate reservoir is very sensitive to several reservoir parameters, such as wellbore pressure and stratum porosity and permeability.

郭永怀文集

Stabilizing to disruptive transition of focal adhesion response to mechanical forces

Strong mechanical forces can, obviously, disrupt cell-cell and cell-matrix adhesions, e.g., cyclic uniaxial stretch induces instability of cell adhesion, which then causes the reorientation of cells away from the stretching direction. However, recent experiments also demonstrated the existence of force dependent adhesion growth (rather than dissociation). To provide a quantitative explanation for the two seemingly contradictory phenomena, a microscopic model that includes both integrin-integrin interaction and integrin-ligand interaction is developed at molecular level by treating the focal adhesion as an adhesion cluster. The integrin clustering dynamics and integrin-ligand binding dynamics are then simulated within one unified theoretical frame with Monte Carlo simulation. We find that the focal adhesion will grow when the traction force is higher than a relative small threshold value, and the growth is dominated by the reduction of local chemical potential energy by the traction force. In contrast, the focal adhesion will rupture when the traction force exceeds a second threshold value, and the rupture is dominated by the breaking of integrin-ligand bonds. Consistent with the experiments, these results suggest a force map for various responses of cell adhesion to different scales of mechanical force. PMID: 20542514

冲压发动机喷气催化复合效应研究

在高超声速飞行条件下，流入冲压发动机燃烧室并降至低速的空气的温度随飞行马赫数增加而愈来愈高。燃料与高温空气混合燃烧释放的化学能中的一部分将转化为解离能。这些解离能在长度受限的尾喷管中难以充分复合形成推力，使冲压发动机推力在高超声速范围内随飞行马赫数增大而下降，难以满足高超声速飞行器的推进要求。 与亚燃冲压发动机相比，流入超燃冲压发动机燃烧室的空气的温度在同样飞行马赫数条件下将明显降低，上述困难可大大缓解。然而目前超燃冲压发动机还存在关键性难点有待克服。若保持现有亚燃冲压发动机的吸气与燃烧方式，通过催化促进燃气解离组分在尾喷管膨胀过程中复合，可以增大冲压发动机的推力，满足高超声速飞行器的推进要求，为高超声速飞行器推进提供新的选择。 本论文主要研究内容如下： （1） 研究了亚燃冲压发动机燃烧室内燃气解离能与飞行马赫数的关系。通过对冻结流、平衡流和有限化学反应速率的流动的数值计算，确定了回收解离能增大推力的潜力。 （2） 以双爆轰技术为基础，建立起一套地面燃气产生装置。所产生的燃气的组分、温度和压力均与冲压发动机在高空飞行时燃气完全相同。调试出总温3200K、总压20Bar（对应来流马赫数6）和试验时间17.5ms以及总温4000K、总压5Bar（对应来流马赫数8）和试验时间12.5ms两种状态参数的试验用燃气。 （3） 建立了基于动量守恒原理的通过皮托管测压力换算推力的测量方法。对催化复合增大推力的实验而言，一般要进行特定流动条件下喷水与未喷水两种情况下推力大小的比较，其精度可以达到2%甚至更高。 （4） 完成了尾喷管喉道下游管壁喷水试验，成功释放出高温燃气中的解离能，有效增大了推力，证实了催化增推的想法是可行的。在来流马赫数6的条件下获得了11.0%的推力增量；在来流马赫数8.0的条件下也获得了11.7%的推力增加。

Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion

Two-dimensional (2D) kinetics of receptor-ligand interactions governs cell adhesion in many biological processes. While the dissociation kinetics of receptor-ligand bond is extensively investigated, the association kinetics has much less been quantified. Recently receptor-ligand interactions between two surfaces were investigated using a thermal fluctuation assay upon biomembrane force probe technique (Chen et al. in Biophys J 94:694-701, 2008). The regulating factors on association kinetics, however, are not well characterized. Here we developed an alternative thermal fluctuation assay using optical trap technique, which enables to visualize consecutive binding-unbinding transition and to quantify the impact of microbead diffusion on receptor-ligand binding. Three selectin constructs (sLs, sPs, and PLE) and their ligand P-selectin glycoprotein ligand 1 were used to conduct the measurements. It was indicated that bond formation was reduced by enhancing the diffusivity of selectin-coupled carrier, suggesting that carrier diffusion is crucial to determine receptor-ligand binding. It was also found that 2D forward rate predicted upon first-order kinetics was in the order of sPs > sLs > PLE and bond formation was history-dependent. These results further the understandings in regulating association kinetics of surface-bound receptor-ligand interactions.

The Experimental and Numerical Studies on Gas Production from Hydrate Reservoir by Depressurization

A set of experimental system to study hydrate dissociation in porous media is built and some experiments on hydrate dissociation by depressurization are carried out. A mathematical model is developed to simulate the hydrate dissociation by depressurization in hydrate-bearing porous media. The model can be used to analyze the effects of the flow of multiphase fluids, the kinetic process and endothermic process of hydrate dissociation, ice-water phase equilibrium, the variation of permeability, convection and conduction on the hydrate dissociation, and gas and water productions. The numerical results agree well with the experimental results, which validate our mathematical model. For a 3-D hydrate reservoir of Class 3, the evolutions of pressure, temperature, and saturations are elucidated and the effects of some main parameters on gas and water rates are analyzed. Numerical results show that gas can be produced effectively from hydrate reservoir in the first stage of depressurization. Then, methods such as thermal stimulation or inhibitor injection should be considered due to the energy deficiency of formation energy. The numerical results for 3-D hydrate reservoir of Class 1 show that the overlying gas hydrate zone can apparently enhance gas rate and prolong life span of gas reservoir.

藻胆体的光谱特性和光能传递

一，螺旋藻藻胆体光谱特性及其光能传递的研究 1，完整藻胆体与解离藻胆体吸收光谱的比较研究 对螺旋藻完整藻胆体和解离藻胆体的吸收光谱中进行了比较研究。随着PBS逐渐解离，其吸收光谱表现出如下变化特点：在紫外区，吸收峰始终位于355nm，尖形峰逐渐变成钝形峰;在红区，完整藻胆体和解离藻胆体都有很强的光吸收，吸收峰呈平顶状，其半带宽逐渐变小，紫外区与红区相对吸收强度比值逐渐变小，四组导数吸收光谱中的小峰数目越来越少。室温荧光发射光谱表明，PBS在低于0.9mol/L的磷酸缓冲液中变得不稳定，并开始逐渐解离，解离的PBS与完整的PBS相比，其荧光发射峰逐渐蓝移。 2，藻胆体在解离过程中荧光发射和光能传递的研究 完整藻胆体的室温荧光发射光谱中只有一个峰，在678nm。说明在完整藻胆体中，光能传递效率高。在77K荧光发射光谱中，完整藻胆体只有一个峰，位于682nm，这是L_(cm)(TE_1)的荧光峰;严重解离的藻胆体的主峰在656nm，是PC的荧光;在679nm有一个小峰，是APC-B的荧光(TE_2)。据此，我们提出螺旋藻藻胆体的光能传递链为：(此处表从略，见全文) 二，螺旋藻藻胆体核心及其与藻蓝蛋白的重组 PC+core混合物，浓缩重组48h后，其室温荧光发射峰位于663nm，与PC的室温荧光发射峰643nm和PC+core混合物（未重组）的室温荧光发射峰648nm相比，说明部分APC与部分PC发生了重组，使部分PC吸收的光能传递给了APC，使荧光发射峰红移;与藻胆体核心室温荧光发射峰664nm相比，则非常接近，说明重组效果较好。PC+core混合物（未重组），其77K荧光发射光谱中有两个峰：654nm,679nm,分别是PC,APC-B的荧光峰，F679/F654的比值为32.0%。我们以F679/F654比值的变化来判断PC与core是否发生了重组。PC+core混合物，经48h浓缩重组后，77K荧光发射光谱中有F657,F679两个峰，F679/F654的比值则为45.9%，比未重组的混合物32.0%升高了，说明部分PC与core发生了重组，部分PC吸收的光能传递给了APC和APC-B，使F679加强，F654减弱。 三，螺旋藻藻胆体一类囊体膜光谱特性与光能传递的研究 藻胆体一类囊体膜的吸收光谱，室温荧光发射光谱和77K荧光发射光谱表明：藻胆蛋白能将捕获的光能传递给叶绿素a，叶绿素a捕获的光能不能逆传给藻胆蛋白。 四，藻胆体一类囊体膜的重组 藻胆体一类囊体膜的吸收光谱说明，一部分被洗下来的PBS能重新结合到类囊体膜上，但并没有达到100%的重组。 五，整体螺旋藻光谱特性及其光能传递的研究 整体螺旋藻光谱特性与PBS-类囊体膜的光谱特性极为相似，表现出同样的规律：PBS的吸收面积与叶绿素a相比，叶绿素a的吸收是主要的。 从PBS-类囊体膜和整体螺旋藻的吸收光谱，室温荧光发射光谱，77K荧光发射光谱的研究中可知，二者表现出极为相似的规律：PBS藻胆蛋白捕获的光能能传递给叶绿素a，叶绿素a捕获的光能不能逆传给PBS藻胆蛋白。主要的捕光物质是叶绿素a。 另外，我们还对Spirulina platensis 6 and Spirulina maxima的藻胆体在解离过程中的荧光发射和光能传递进行了研究，表现规律与Spirulina platensis相同。

Frog albumin is expressed in skin and characterized as a novel potent trypsin inhibitor

A novel potent trypsin inhibitor was purified and characterized from frog Bombina maxima skin. A full-length cDNA encoding the protein was obtained from a cDNA library constructed from the skin. Sequence analysis established that the protein actually comprises three conserved albumin domains. B. maxima serum albumin was subsequently purified, and its coding cDNA was further obtained by PCR-based cloning from the frog liver. Only two amino acid variations were found in the albumin sequences from the skin and the serum. However, the skin protein is distinct from the serum protein by binding of a haem b (0.95 mol/mol protein). Different from bovine serum albumin, B. maxima albumin potently inhibited trypsin. It bound tightly with trypsin in a 1: 1 molar ratio. The equilibrium dissociation constants (K-D) obtained for the skin and the serum proteins were 1.92 x 10(-9) M and 1.55 x 10(-9) M, respectively. B. maxima albumin formed a noncovalent complex with trypsin through an exposed loop formed by a disulfide bond (Cys(53)-Cys(62)), which comprises the scissile bond Arg(58)(P-1)-His(59)(P-1'). No inhibitory effects on thrombin, chymotrypsin, elastase, and subtilisin were observed under the assay conditions. Immunohistochemical study showed that B. maxima albumin is widely distributed around the membranes of epithelial layer cells and within the stratum spongiosum of dermis in the skin, suggesting that it plays important roles in skin physiological functions, such as water economy, metabolite exchange, and osmoregulation.

A STUDY ON THE EFFECT OF LESIONS OF AREA-7 OF THE PARIETAL CORTEX ON THE SHORT-TERM VISUAL SPATIAL MEMORY OF RHESUS-MONKEYS (MACACA-MULATTA)

This research is focused on the contribution of area 7 to the short-term visual spatial memory. Three rhesus monkeys (Macaca mulatta) were trained in the direct delayed response task in which 5 delay intervals were used in each session. When each monkey reached the criterion of 90% correct responses in 5 successive sessions, two monkeys underwent a surgery while the other one received a sham operation as a control. In the first stage of the surgery, bilateral areas 7a, 7b and 7ip of the parietal cortex of two monkeys were precisely lesioned. After 7 days of recuperation, the monkeys were required to do the same task. The average percentage of correct responses in the lesioned animals decreased from 94.7% to 89.3% and 93.3% to 82.0% respectively (no significance, P > 0.05, n = 2). In addition, the monkeys' complex movements were mildly impaired. The lesioned monkeys were found to have difficulty picking up food from the wells. In the second stage, bilateral area 7m was lesioned. In the 5 postoperative sessions, the average percentage of correct responses in one monkey, with a relatively precise 7m lesion, decreased from 94.7% to 92.2% (no significance, P > 0.05), while the other monkey, with widely spread necrosis of lateral parietal cortex, showed an. obvious decline in performance, but still over the chance level. After 240 trials this monkey reattained the normal criterion. The results of this research suggest that the lesions of area 7 of the parietal cortex did not significantly affect the short-term visual spatial memory, which has been shown to be sensitive to lesions of the prefrontal cortex; they also support the notion of dissociation of spatial functions in the prefrontal and parietal cortices.

Hybrid bulk heterojunction solar cells from a blend of poly(3-hexylthiophene) and TiO2 nanotubes

Hybrid bulk heterojunction solar cells based on blend of poly(3-hexylthiophene) (P3HT) and TiO2 nanotubes or dye(N719) modified TiO2 nanotubes were processed from solution and characterized to research the nature of organic/inorganic hybrid materials. Compared with the pristine polymer P3HT and TiO2 nanoparticles/P3HT solar cells, the TiO2 nanotubes/P3HT hybrid solar cells show obvious performance improvement, due to the formation of the bulk heterojunction and charge transport improvement. A further improvement in the device performance can be achieved by modifying TiO2 nanotube surface with a standard dye N719 which can play a role in the improvement of both the light absorption and charge dissociation. Compared with the non-modified TiO2 nanotubes solar cells, the modified ones have better power conversion efficiency under 100 mW/cm(2) illumination with 500W Xenon lamp. (C) 2008 Elsevier B. V. All rights reserved.

Zn2SiO4/ZnO Core/Shell Coaxial Heterostructure Nanobelts Formed by an Epitaxial Growth

Si-doped ZnO can be synthesized on the surface of the early grown Zn2SiO4 nanostructures and form core/ shell coaxial heterostructure nanobelts with an epitaxial orientation relationship. A parallel interface with a periodicity array of edge dislocations and an inclined interface without dislocations can be formed. The visible green emission is predominant in PL spectra due to carrier localization by high density of deep traps from complexes of impurities and defects. Due to band tail localization induced by composition and defect fluctuation, and high density of free-carriers donated by doping, especially the further dissociation of excitons into free-carriers at high excitation intensity, the near-band-edge emission is dominated by the transition of free-electrons to free-holes, and furthermore, exhibits a significant excitation power-dependent red-shift characteristic. Due to the structure relaxation and the thermalization effects, carrier delocalization takes place in deep traps with increasing excitation density. As a result, the green emission passes through a maximum at 0.25I(0) excitation intensity, and the ratio of the violet to green emission increases monotonously as the excitation laser power density increases. The violet and green emission of ZnO nanostructures can be well tuned by a moderate doping and a variation in the excitation density.

Effect of annealing on photoluminescence properties of neon implanted GaN

The effect of thermal annealing on the luminescence properties of neon implanted GaN thin films was studied. Low temperature photoluminescence (PL) measurements were carried out on the samples implanted with different doses ranging from 10(14) to 9 x 10(15) cm(-2) and annealed isochronally at 800 and 900 degrees C. We observed a new peak appearing at 3.44 eV in the low temperative PL spectra of all the implanted samples after annealing at 900 degrees C. This peak has not been observed in the PL spectra of implanted samples annealed at 800 degrees C except for the samples implanted with the highest dose. The intensity of the yellow luminescence (YL) band noticed in the PL spectra measured after annealing was observed to decrease with the increase in dose until it was completely suppressed at a dose of 5 x 10(15) cm(-2). The appearance of a new peak at 3.44 eV and dose dependent suppression of the YL band are attributed to the dissociation of VGaON complexes caused by high energy ion implantation.

Effect of ultraviolet light on hybrid zinc oxide polymer bulk heterojunction solar cells

Compared to conjugated polymer poly[2-methoxy-5- (3',7'-dimethyloctyloxy)-1,4-phenylenevinylene] (MDMO-PPV) solar cells, bulk heterojunction solar cells composed of zinc oxide (ZnO) nanocrystals and MDMO-PPV have a better energy conversion efficiency, However, ultraviolet (UV) light deteriorates the performance of solar cells composed of ZnO and MDMO-PPV. We propose a model to explain the effect of UV illumination on these ZnO:MDMO-PPV solar cells. According to this model, the degradation from UV illumination is due to a decrease of exciton dissociation efficiency, Our model is based on the experimental results such as the measurements of current density versus voltage, photoluminescence, and photocurrent.

Structural evaluation of polycrystalline silicon thin films by hot-wire-assisted PECVD

Tungsten wires were introduced into a plasma-enhanced chemical vapor deposition (PECVD) system as a catalyzer: we name this technique 'hot-wire-assisted PECVD' (HW-PECVD). Under constant deposition pressure (p(g)), gas flow ratio and catalyzer position, the effects of the hot wire temperature (T-f) on the structural properties of the poly-Si films have been characterized by X-ray diffraction (XRD), Raman scattering and Fourier-transform infrared (FTIR) spectroscopy. Compared with conventional PECVD, the grain size, crystalline volume fraction (X-e) and deposition rate were all enhanced when a high T-f was used. The best poly-Si film exhibits a preferential (220) orientation, with a full width at half-maximum (FWHM) of 0.2 degrees. The Si-Si TO peak of the Raman scattering spectrum is located at 519.8 cm(-1) with a FWHM of 7.1 cm(-1). The X-c is 0.93. These improvements are mainly the result of promotion of the dissociation of SiH4 and an increase in the atomic H concentration in the gas phase. (C) 2001 Elsevier Science B.V. All rights reserved.