现代黄河三角洲湿地景观变化及其对水禽生境的影响

本文研究的开展主要源于国家海洋局海洋公益性行业科研专项经费项目“渤海典型区域的海洋多过程数据整合研究”课题支撑。在湿地日益退化丧失的全球背景下，本文选择沉积年代较轻、生态环境比较脆弱，且当前人类活动不断加剧的现代黄河三角洲为研究区，采用1999、2001、2004和2008年的Landsat5 TM遥感影像，选择丹顶鹤和黑嘴鸥为指示物种，结合野外实地调查和历史数据资料的整理和分析，以及黄河三角洲自然保护区对鸟类的监测记录结果，开展现代黄河三角洲湿地景观变化及其对水禽生境的影响研究。 论文的主要研究结果如下： 1、建立了服务于黄河三角洲湿地水禽生境保护的湿地景观分类体系 在系统分析研究区域湿地水禽生活习性和生态分布特征的基础上，从鸟类生境选择的概念出发，建立了服务于黄河三角洲湿地水禽生境保护的湿地景观分类体系。该分类体系一共分为4级，其中，第三级分类系统下天然湿地的划分是从鸟类选择的“大生境”角度出发，即根据区域地表类型，进一步将海洋/海岸湿地和内陆三角洲湿地划分成浅海水域、潮间淤泥滩、潮沟、河口水域、潮间盐沼、河流湿地和沼泽湿地7类。而第四级分类系统下天然湿地的划分则是从鸟类选择的“小生境”（即对水、食物、隐蔽物、干扰等的需求）角度出发，并按照水文和典型植被类型，进一步将天然湿地的三级类型划分为碱蓬沼泽、芦苇沼泽、柽柳灌丛沼泽等33类。 2、系统研究了现代黄河三角洲湿地景观格局的动态变化和影响因素 根据景观生态学理论，选取用于分析的景观格局指数，并从湿地景观类型的组成特征变化、面积变化、形状特征变化、空间配置特征变化四个方面分析在黄河断流前到其恢复正常行水至今的时间里三角洲湿地景观格局的动态变化，研究结果表明：在1999~2008年的9年间，天然湿地构成了现代黄河三角洲景观的主体，约占研究区域景观总面积的65%~75%，决定了该地区湿地的主要结构和功能。但随着人类对土地资源利用方式的不断改变和强度的提高，天然湿地景观呈萎缩退化趋势，9年内共减少338.44 km2，约占研究区景观总面积的8.90%。与此同时，盐田、养殖池塘等人工湿地和耕地等非湿地面积不断扩张，人工湿地面积增加量约为242.08km2，约占研究区景观总面积的6.37%，非湿地面积增加量约为96.36 km2，约占研究区景观总面积的2.53%。从一些重要的景观指数的计算结果来看，研究区域整体景观斑块数量呈增加趋势，平均斑块大小随之减小，景观边界密度大小、聚集度指数也呈下降趋势，表明从1999到2008年研究区域整体景观被边界分割的程度加深，整体景观中的斑块在空间分布上隔离程度加重，开始由大片连续分布向小块离散分布转变，景观的破碎化程度加重。 湿地景观格局变化的驱动机制分析表明，影响研究区湿地景观变化的主要因素包括三个方面，即：黄河水沙条件的驱动力、湿地自身演替驱动力和人为驱动力。在1999~2008年的9年间，黄河虽然恢复正常行水，不再断流，但入海泥沙量的明显减少使得除现行河口处沙嘴继续向海缓慢延伸外，黄河三角洲总体上处于侵蚀状态。湿地自身的演替主要体现在湿地由裸露淤泥质滩涂向发育典型湿地植物群落的顺向演替，以及发育有典型湿地植物群落的湿地向裸露淤泥质滩涂逆向演替两个方面。在1999~2008年间9年里，湿地顺向发育演替面积约130.64 km2，而逆向发育演替面积约为99.51 km2。人类活动一直以来都是影响黄河三角洲湿地景观变化不可忽视的营造力。大规模的滩涂围垦和养殖、农业用地的不断开垦和扩张，以及密集的油田开发活动不仅缩减了原本生长着芦苇茅草等植物的天然湿地资源，而且加重了三角洲湿地景观的破碎程度。 3、在整个研究区域尺度上深刻分析了丹顶鹤和黑嘴鸥潜在生境的适宜性 根据黄河三角洲自然保护区关于鸟类的监测记录资料，在深入分析湿地水禽生活习性和分布特征的基础上，选择丹顶鹤和黑嘴鸥为指示物种，从鸟类生境选择自身的特征出发，确定地表类型、水、食物、隐蔽物和干扰为影响两种鸟类类群进行生境选择的因素，并构建生境适宜性评价模型，在整个研究区域尺度上对两种鸟类类群的潜在生境进行适宜性评价和动态变化分析。研究结果表明，在不考虑居民地、油田作业用地和道路等干扰因子对生境产生的破碎化效应影响时，适宜丹顶鹤和黑嘴鸥生存的生境类型在1999~2008年间占研究区整体景观总面积的比重均在30%以上，其中天然湿地约占适宜生境类型面积的95%，可利用的人工湿地比重极小，但呈缓慢增加趋势，且由于可利用的天然湿地生境类型的逐年减少使得适宜两种鸟类类群的生境类型总面积总体上呈萎缩趋势。在考虑居民地、油田作业用地和道路等干扰因子对生境产生的破碎化效应影响时，适宜丹顶鹤和黑嘴鸥生存的生境类型在1999~2008年间占研究区域整体景观总面积的百分比由之前接近40%下降到20%以下，面积减少量均在600 km2以上。 4、系统分析了研究区湿地景观变化对水禽生境的影响 从研究区湿地景观基质的面积变化、形状和空间邻接关系变化、破碎化等三个方面分析了研究区湿地景观变化对丹顶鹤和黑嘴鸥两种鸟类类群生境的影响，结果表明，湿地景观基质中天然湿地的变化与丹顶鹤、黑嘴鸥适宜生境的大小和空间分布密切相关。在1999~2008年间，随着天然湿地面积的减少，适宜以上两种鸟类类群的生境面积也随之减少，两者呈正相关关系；在1999~2008年间，研究区天然湿地和人工湿地景观基质整体的形状指数均在1.0附近，表明湿地景观基质整体上的边缘效应较小，但建设用地、农林用地等非湿地景观与丹顶鹤和黑嘴鸥适宜生境类型中的主导类型——潮间淤泥滩、潮间盐沼和沼泽湿地等三种景观类型的频繁邻接，对于保护两种鸟类类群十分不利，应积极采取相应的措施加强保护力度；在1999~2008年间，研究区景观整体，以及湿地景观基质结构的破碎化，导致鸟类可利用的天然湿地和人工湿地景观内部斑块之间的连接程度下降，从而使得丹顶鹤、黑嘴

47,XY,+dcl(9)(q34)wcp(9+)/46,XY伴隐睾一例

患儿男,10岁,因"双侧隐睾"就诊.患儿系足月、顺产、5岁时发现头颅大,经医院诊断为脑积水.几岁时做双侧隐睾手术.患儿身高120 cm.头颅发育不规则,颜面不对称,自小严重的智力低下,说话不清.

Comparison of Pax1/9 locus reveals 500-Myr-old syntenic block and evolutionary conserved noncoding regions

Identification of conserved genomic regions within and between different genomes is crucial when studying genome evolution. Here, we described regions of strong synteny conservation between vertebrate deuterostomes (tetrapods and teleosts) and invertebrat

Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima

Amphibian skin is a rich resource of antimicrobial peptides, like maximins and maximin Hs from frog Bombina maxima. Novel cDNA clones encoding a precursor protein, which comprises a novel maximin peptide (maximin 9) and reported maximin H3, were isolated from two constructed skin cDNA libraries of B. maxima. The predicted primary structure of maximin 9 is GIGRKFLGGVKTTFRCGVKDFASKHLY-NH2. A surprising substitution is at position 16, with a free cysteine in maximin 9 rather than usual conserved glycine in other reported maximins. Maximin 9, the homodimer form and its Cys(16) to Gly(16) mutant were synthesized and their antimicrobial activities were evaluated. Unlike previously reported maximin 3, the tested bacterial and fungal strains were resistant to maximin 9, its homodimer and the Cys(16) to Gly(16) mutant (with MICs > 100 mu M). On the other hand, interestingly, while eight clinical Mollicutes strains were generally resistant to maximin 9 homodimer and its Cys(16) to Gly(16) mutant, most of them are sensitive to maximin 9 at a peptide concentration of 30 mu M, especially in the presence of dithiothreitol. These results indicate that the presence of a reactive Cys residue in maximin 9 is important for its antimycoplasma activity. The diversity of antimicrobial peptide cDNA structures encountered in B. maxima skin cDNA libraries and the antimicrobial specificity differences of the peptides may reflect well the species' adaptation to the unique microbial environments. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Evolutionary history of the mtDNA 9-bp deletion in Chinese populations and its relevance to the peopling of east and southeast Asia

In total, 1218 Chinese from twelve ethnic groups and nine Han geographic groups were screened for the mtDNA 9-bp deletion motif. The frequency of the 9-bp deletion in all samples was 14.7% but ranged from 0% to 32% in the various ethnic groups. Three individuals had a triplication of the 9-bp segment. Phylogenetic and demographic analyses of the mtDNA hypervariable segment 1 (HVS1) sequences suggest that the 9-bp deletion occurred more than once in China. The majority of the Chinese deletion:haplotypes (about 90%) have a common origin as a mutational event following an initial expansion of modem humans in eastern Asia. Other deletion haplotypes and the three haplotypes with a 9-bp triplication may have arisen independently in the Chinese, presumably by replication error. HVS1 haplotype analysis suggests two possible migration routes of the 9-bp deletion in east and southeast Asia. Both migrations originated in China with one route leading to the Pacific Islands via Taiwan, the other to southeast Asia and possibly the Nicobar Islands. Along both routes of peopling, a decrease in HVS1 diversity of the mtDNA haplotypes is observed. The "Polynesian motif (16217T/C, 16247A/G, and 16261C/T)" and the 16140T/C, 16266C/A, or C/G polymorphisms appear specific to each migration route.

Frequency of the mtDNA 9-bp deletion in Chinese ethnic groups

The 9-bp deletion in the COII/tRNA(Lys) intergenic region (region V) of human mitochondrial DNA was screened in 1521 Chinese from 16 ethnic groups and 9 Hen geographic groups. The highest frequency was found in populations of Miao (32.4%) and Bouyei (30.8

Delta EEG Activity in Left Orbitofrontal Cortex in Rats Related to Food Reward and Craving

眶额叶皮质与中脑边缘多巴胺奖赏系统有着复杂的相互纤维联系.先前的研究探讨了药物成瘾过程中眶额叶皮质的脑电活动.在本实验中,将探讨食物奖赏和渴求过程中该皮质的脑电活动.实验采用了两个环境:对照环境和食物刺激相关的环境.首先,训练大鼠在食物刺激相关的环境中吃巧克力花生豆,而后在该环境中设置两种不同的刺激方式:能看到和闻到但不能吃到(渴求实验),或者仍旧可以吃到巧克力花生豆(奖赏实验):同时进行左侧眶额叶皮质的脑电记录.结果发现,在食物刺激相关的环境中大鼠Delta频段(2-4Hz)的脑电活动与食物刺激显著相关,此外,与在对照环境中相比,其相对功率在食物渴求时下降而在食物奖赏时升高.本实验表明,食物相关的奖励可以改变大鼠眶额叶皮质的脑电活动,而且,Delta频段的脑电活动能够作为监测该奖励的一个指标.

中国9种嗜子宫线虫系统发育的初步研究

为了探讨鱼类寄生嗜子宫线虫的系统发育关系,测定了8种嗜子宫线虫的ITS rDNA(核糖体转录内间隔区核 糖核酸)序列和9种嗜子宫线虫的18S rDNA(小亚基核糖体核糖核酸)部分序列,并构建了18S rDNA序列的系统发 育树。在比较和分析ITS rDNA和18S rDNA两种分子标记对嗜子宫科线虫系统发育适用性的基础上,分析了嗜子 宫线虫的系统发育关系。结果表明:中国嗜子宫线虫是单系起源;黄颡鱼似嗜子宫线虫、赣州似嗜子宫线虫和棍头 嗜子宫线虫亲缘关系非常接近,可能是较晚形成的种;似嗜子宫线虫属可能应该被

中国鲤科鱼类染色体组型的研究——Ⅸ.鳊亚科9种鱼和鲴亚科1种鱼的染色体组型

<正> 我们曾报道过分布于湖北省的鳊、鲴亚科鱼类的核型。本文继续报道采自四川和广东省的鳊、鲴亚科另10种鱼的核型考察结果。材料和方法四川半(歺又鱼)由于采集困难,仅分析了一尾雄鱼。其余9种鱼均分析了3—5尾,包括雌雄两种性别(表1)。染色体标本的制备采用体外短期培养肾细胞制片法(采自四川的鱼),或肾细胞直接制片法(采自广东的鱼)。根据我们实验室以前的工作,两种方法的核型分析结果基本上一致。每种鱼计数60—170个细胞确定其2n数。染色体分组按Levan氏的标准,核型分析方法和臂数计算均同以前的报道。核型图

Molecular cloning and characterization of common carp (Cyprinus carpio L.) TCR gamma and CD3 gamma/delta chains

Partial cDNA sequences of TCR gamma and CD3 gamma/delta were isolated from the thymus of common carp (Cyprinus carpio L.) by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp TCR gamma and CD3 gamma/delta were obtained by means of 3' RACE and 5' RACE, respectively. The full length of carp TCR gamma chain is 1368 bp and encodes 326 amino acids including a signal peptide region of 19 amino acids and a transmembrane region of 23 amino acids at the C-terminal region from aa 291 to 313. The V region of carp TCR gamma contains 109 amino acids, the core motif FGXG in J segment was also found in carp TCR gamma. The C region of carp TCR gamma contains the characteristic CX6PX6WX45C motif. The CP region of carp TCR C gamma contains 37 amino acids. The full length of carp CD3 gamma/delta is 790 bp and encodes 175 amino acids including a signal peptide region of 17 amino acids and a transmembrane region of 23 amino acids from aa 93 to 115. Similar to other known CD3 gamma/delta s, four cysteine residues in the extracellular domain and an immunoreceptor tyrosine-based activation motif ITAM (YxxL/Ix6-8YxxL/I) in the intracellular domain are also included in carp CD3 gamma/delta. Differing from other known CD3 gamma/delta s, carp CD3 gamma/delta tacks the CXXCXE motif in the extracellular domain. RTPCR analysis demonstrated that the expression of TCR gamma gene was mainly in the thymus and gill of 6-month carp, but in 18-month carp, TCR gamma gene was detected in all the examined tissues. The expression of CD3 gamma/delta gene was detected in all examined tissues of 6 and 18-month carp; among them, the highest expression level was in the thymus of 6-month carp. In situ hybridization showed that CD3 gamma/delta-expressing cells were widely distributed in the head kidney, spleen and kidney of carp, whereas in the thymus, they were densely distributed in the lymphoid outer zone and scattered in the epithelioid inner zone. (c) 2007 Published by Etsevier Ltd.

Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells

A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Delta psi m collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-kappa B activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells.