Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation

In this paper, we study the ability of DNA-PK-deficient (M059J) and -proficient (M059K) cells to undergo the rate of cellular proliferation, cell cycle distribution and apoptosis after 10 Gy X-ray irradiation, and the role of DNA-PK in radiosensitivity. The results showed that M059J cells exhibited hyper-radiosensitivity compared with M059K cells. A strong G2 phase arrest was observed in M059J cells post irradiation. Significant accumulation in the G2 phase in M059J cells was accompanied by apoptosis at 12 h. Altogether, the data suggested that DNA-PK may have two roles in mammalian cells after DNA damage, a role in DNA DSB repair and a second role in DNA-damaged cells to traverse a G2 checkpoint, by which DNA-PK may affect cellular sensitivity to ionizing radiation. 地址: [Li Ning; Zhang Hong; Wang Yanling; Hao Jifang] Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China; [Li Ning; Zhang Hong; Wang Yanling; Hao Jifang] Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China; [Li Ning; Wang Yanling] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Wang Xiaohu] Gansu Tumor Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China

Properties of the beta-delayed proton decay of Ho-146

The proton-rich isotope Ho-148 was produced via the fusion-evaporation reaction Mo-92 (Ni-58, 3p1n). The beta-delayed proton decay of Ho-146 was studied by proton-gamma coincidence measurements using a He-jet tape transport system. The gamma-transitions in Tb-145 following the proton emissions were observed, and the beta-delayed proton branching ratios to the final states in the grand-daughter nucleus Tb-145 were determined. According to the relative branching ratios, the ground-state spin of Ho-146 has been proposed and the possible configuration discussed.

Observation of a Possible New Isomer in Ir-175

The neutron deficient nuclide Ir-175 was produced by irradiation of Nd-146 with 210 MeV Cl-35 via a fusion-evaporation reaction channel. The reaction products were transported to a low-background location using a helium-jet recoil fast-moving tape-transport system for measurement. The experimental devices and data analysis method are introduced. Based on the decay-curve fitting of the beta-delayed gamma ray from Ir-175, realized by the least-square method, a new long-lived isomeric state of Ir-175 is proposed and briefly discussed.

Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks

DNA double-strand breaks (DSBs) are the most deleterious lesion inflicted by ionizing radiation. Although DSBs are potentially carcinogenic, it is not clear whether complex DSBs that are refractory to repair are more potently tumorigenic compared with simple breaks that can be rapidly repaired, correctly or incorrectly, by mammalian cells. We previously demonstrated that complex DSBs induced by high-linear energy transfer (LET) Fe ions are repaired slowly and incompletely, whereas those induced by low-LET gamma rays are repaired efficiently by mammalian cells. To determine whether Fe-induced DSBs are more potently tumorigenic than gamma ray-induced breaks, we irradiated 'sensitized' murine astrocytes that were deficient in Ink4a and Arf tumor suppressors and injected the surviving cells subcutaneously into nude mice. Using this model system, we find that Fe ions are potently tumorigenic, generating tumors with significantly higher frequency and shorter latency compared with tumors generated by gamma rays. Tumor formation by Fe-irradiated cells is accompanied by rampant genomic instability and multiple genomic changes, the most interesting of which is loss of the p15/Ink4b tumor suppressor due to deletion of a chromosomal region harboring the CDKN2A and CDKN2B loci. The additional loss of p15/Ink4b in tumors derived from cells that are already deficient in p16/Ink4a bolsters the hypothesis that p15 plays an important role in tumor suppression, especially in the absence of p16. Indeed, we find that reexpression of p15 in tumor-derived cells significantly attenuates the tumorigenic potential of these cells, indicating that p15 loss may be a critical event in tumorigenesis triggered by complex DSBs.

缺中子奇质量数Au核高自旋态能级结构研究

利用能量为164-180MeV的35Cl束流，通过重离子核反应149Sm(35Cl,5n) 研究了179Au的高自旋态能级结构。实验进行了γ射线的激发函数、X-γ和γ-γ-t符合测量。基于实验测量结果，首次建立了179Au的1/2[660](πi13/2)转动带。结合已有的实验数据，着重讨论了奇-A Au核中1/2[660](πi13/2) 转动带的形变和带头激发能随中子数的变化。用能量为140MeV的29Si束流轰击159Tb金属靶，布居了183Au核的高自旋态。实验中要求至少有3个高纯锗和2个BGO探测器同时点火，在此符合条件下，记录高纯锗探测器探测到的γ射线的能量和相对时间、BGO探测到的γ射线的总能量和多重性。通过对实验数据的分析，扩展并更新了183Au的能级纲图。首次建立了183Au的πi13/2转动带的能量非优先带。分析并讨论了缺中子奇-A Au中πh9/2转动带的能量非优先带和πf7/2转动带间的相互作用

缺中子核素133Sm、133Pr和93Pd的衰变

本硕士沦文籍助兰州重离子加速器国家实验室分离扇聚焦回旋加速器（SFC）提供的重离子束通过全融合蒸发反应产生远离口稳定线的缺中子核素，并用本课题组发展和建立的氦喷嘴快速带传输系统以及多重符合测量装置分离、测量核素来研究其衰变性质。在实验准备期间，曾采用~(252)Cf源进行了氦喷嘴（I－I eje咧央速带传输系统效率的测定。给出了PbI_2，PbCl_2，NaCl三种无机欲添加剂的传输效率，以及传输效率随目标核质量变化的初步结果。而后完成了以下两项物理实验：~(113)Sm的（EC＋β~+）衰变纲图和133Pr的同核异能态的研究：用SFC引出的~(40)Ca~(12+)束流轰击96%Ru的浓缩同位素靶，采用氦喷嘴带传输系统和x-γ与γ-γ符合测量方法，首次建议了~(133)Sm的简单的（EC＋β~+）衰变纲图并测得了其β衰变半衰期。~(133)Sm是目前发表过（EC＋β~+）衰变纲图的最轻的钐（Sm的缺中子核。由于Ru靶中含有~(98-102)Ru的成分，同时产生了~(133)Pr，并首次测定了~(133)Pr 11／2~－同核异能态的寿命为（l.1±0.2）s。为Pr奇A核11／2~-同核异能态的数据作了重要的补充，汇编成Pr奇A核的11／2一同核异能态和与之相关的3／2~＋，5／2~+和7／2~+能级的系统性数据。用单粒子模型理论拟合从中提取了~(131,133,135,137)Pr的11／2~-同核异能态的约化跃迁概率的实验值，并与Weisskopf近似估计进行了比较。2.~(93)Pd的β缓发质子衰变：通过~(58)Ni（~(40)Ca，3n2p）反应合成β缓发质了先驱核~(93)Pd，采用氦喷嘴带传输系统加p-γ符合观测到了它的β缓发质子衰李，测得其半衰期为1.3±0.2s。采用统计模型计算拟合了实验测得的口缓发质子能谱和布居到质子发射体子核不同终态的分支比，首次由实验数据出发初步指认了93Pd的基态自旋-宇称为9／2~±。同时与采用Woods-Saxon Strutinsky方法计算~(93)Pd的核位能面得到的结果进行了比较。计算结果支持对~(93)Pd基态自旋一宇称为9／2~+的实验指认。

缺中子奇质量数Au核高自旋态能级结构研究

利用能量为164-180MeV的35Cl束流，通过重离子核反应149Sm(35Cl,5n) 研究了179Au的高自旋态能级结构。实验进行了γ射线的激发函数、X-γ和γ-γ-t符合测量。基于实验测量结果，首次建立了179Au的1/2[660](πi13/2)转动带。结合已有的实验数据，着重讨论了奇-A Au核中1/2[660](πi13/2) 转动带的形变和带头激发能随中子数的变化。用能量为140MeV的29Si束流轰击159Tb金属靶，布居了183Au核的高自旋态。实验中要求至少有3个高纯锗和2个BGO探测器同时点火，在此符合条件下，记录高纯锗探测器探测到的γ射线的能量和相对时间、BGO探测到的γ射线的总能量和多重性。通过对实验数据的分析，扩展并更新了183Au的能级纲图。首次建立了183Au的πi13/2转动带的能量非优先带。分析并讨论了缺中子奇-A Au中πh9/2转动带的能量非优先带和πf7/2转动带间的相互作用

Effects of nitrogen fertilization on soil respiration in temperate grassland in Inner Mongolia, China

Nitrogen addition to soil can play a vital role in influencing the losses of soil carbon by respiration in N-deficient terrestrial ecosystems. The aim of this study was to clarify the effects of different levels of nitrogen fertilization (HN, 200 kg N ha(-1) year(-1); MN, 100 kg N ha(-1) year(-1); LN, 50 kg N ha(-1) year(-1)) on soil respiration compared with non-fertilization (CK, 0 kg N ha(-1) year(-1)), from July 2007 to September 2008, in temperate grassland in Inner Mongolia, China. Results showed that N fertilization did not change the seasonal patterns of soil respiration, which were mainly controlled by soil heat-water conditions. However, N fertilization could change the relationships between soil respiration and soil temperature, and water regimes. Soil respiration dependence on soil moisture was increased by N fertilization, and the soil temperature sensitivity was similar in the treatments of HN, LN, and CK treatments (Q (10) varied within 1.70-1.74) but was slightly reduced in MN treatment (Q (10) = 1.63). N fertilization increased soil CO2 emission in the order MN > HN > LN compared with the CK treatment. The positive effects reached a significant level for HN and MN (P < 0.05) and reached a marginally significant level for LN (P = 0.059 < 0.1) based on the cumulative soil respiration during the 2007 growing season after fertilization (July-September 2007). Furthermore, the differences between the three fertilization treatments and CK reached the very significant level of 0.01 on the basis of the data during the first entire year after fertilization (July 2007-June 2008). The annual total soil respiration was 53, 57, and 24% higher than in the CK plots (465 g m(-2) year(-1)). However, the positive effects did not reach the significant level for any treatment in the 2008 growing season after the second year fertilization (July-September 2008, P > 0.05). The pairwise differences between the three N-level treatments were not significant in either year (P > 0.05).

Fabrication, thermal stability and mechanical properties of novel composite hardmetals obtained by solid-state reaction and hot-pressing sintering

A novel cemented carbides (W0.7Al0.3)C-0.65-Co with different cobalt contents were prepared by solid-state reaction and hot-pressing technique. Hot-pressing technique as a novel technique was performed to fabricate the bulk bodies of the hard alloys. The novel cemented carbides have superior mechanical properties compared with WC-Co. The density, operate cost of the novel material were lower than WC-Co system. The novel materials were easy to process nanoscale sintering and get the rounded particles in the bulk materials. There is almost no eta-phase in the (W0.7Al0.3)C-0.65-Co cemented carbides system although the carbon deficient get the astonished 35% value.

Fabrication, thermal stability and mechanical properties of novel (W0.5Al0.5)C-0.8-Co composite prepared by mechanical alloying and hot-pressing sintering

A novel cemented carbides (W0.5Al0.5)C-0.8-Co with different cobalt contents were prepared by mechanical alloying and hot-pressing technique. Hot-pressing technique as a common technique was performed to fabricate the bulk bodies of the hard alloys. The novel cemented carbides have superior mechanical properties compared to WC-Co. The density, operating cost of the novel material were much lower than WC-Co. There is almost no eta-phase in the (W0.5Al0.5)C-0.8-Co cemented carbides system although the carbon deficient get the value of 20%, and successfully got the nanostructured rounded (W0.5Al0.5)C-0.8 particles.

A novel (W-Al)-C-Co composite cemented carbide prepared by mechanical alloying and hot-pressing sintering

Novel cemented carbides (W0.4Al0.6)C-0.5-Co With different cobalt contents were prepared by mechanical alloying and hot-pressing technique. Hot-pressing technique as a common technique was performed to fabricate the bulk bodies of the hard alloys. The novel cemented carbides have good mechanical properties compared with WC-Co. The density and operation cost of the novel material were much lower than the WC-Co system. It was easy to process submicroscale sintering with the novel materials and obtain the rounded particles in the bulk materials. There is almost no eta-phase in the (W0.4Al0.6)C-0.5-CO cemented carbides system although the carbon deficient obtains the astonishing value of 50%.

Fabrication, microstructure and mechanical properties of novel cemented hard alloy obtained by mechanical alloying and hot-pressing sintering

A novel cemented carbides alloy (W0.4Al0.6)C-0.65-Co were prepared by mechanical alloying and hot-pressing sintering in this work. Hot-pressing (HP) as a common technique was performed to fabricate the bulk bodies of the hard alloys. The hardness, bending strength, density of the novel hard alloy are also tested, and it has superior mechanical properties. The hardness of (W0.4Al0.6)C-0.65-Co hard alloy was very high, and the density, operate cost of the novel material were much lower than WC-Co, more important is the aluminum dissolving is not decrease the strength compared with the WC-Co system. There is almost no eta-phase in the (W0.4Al0.6)C-0.65-Co cemented carbides system even the carbon deficient reaches the astonishing value of 35%. This novel property will give us more choice to design and gain new materials that we needed.

Phenylene vinylene-based electroluminescent polymers with electron transport block in the main chain

We report a new route for the design of soluble phenylene vinylene (PV) based electroluminescent polymers bearing electron-deficient oxadizole (OXD) and triazole (TZ) moieties in the main chains with the aryloxy linkage. Both series of the PV-based polymers were prepared by Wittig reaction. By properly adjusting the OXD and/or TZ content through copolymerization, we can achieve an enhanced balance of hole- and electron injections, such that the device efficiency is significantly improved. Light-emitting diodes fabricated from P1, P2, P3, P4, P5, P6, and P7 with the configuration of Indium-Tin Oxide (ITO)/Poly (styrene sulfonic acid) doped poly (ethylenedioxythiophene) (PEDOT)/polymer/Ca/Al, emit bright green light with the maximum peak around 500 nm. For the device using the optimal polymer (P4) as emitting layer, a maximum brightness of 1300 cd/m(2) at 20 V and a maximum luminance efficiency of 0.325 cd/A can be obtained.

Computer simulation of Zn(II) speciation and effect of Gd(III) on Zn(II) speciation in human blood plasma

The speciation and distribution of Zn(II) and the effect of Gd(III) on Zn(II) speciation in human blood plasma were studied by computer simulation. The results show that, in normal blood plasma, the most predominant species of Zn(II) are [Zn(HSA)] (58.2%), [Zn(IgG)](20.1%), [Zn(Tf)] (10.4%), ternary complexes of [Zn(Cit)(Cys)] (6.6%) and of [Zn(Cys)(His)H] (1.6%), and the binary complex of [Zn(CYS)(2)H] (1.2%). When zinc is deficient, the distribution of Zn(II) species is similar to that in normal blood plasma. Then, the distribution changes with increasing zinc(II) total concentration. Overloading Zn(II) is initially mainly bound to human serum albumin (HSA). As the available amount of HSA is exceeded, phosphate metal and carbonate metal species are established. Gd(III) entering human blood plasma predominantly competes for phosphate and carbonate to form precipitate species. However, Zn(II) complexes with phosphate and carbonate are negligible in normal blood plasma, so Gd(III) only have a little effect on zinc(II) species in human blood plasma at a concentration above 1.0x10(-4) M.

Polymer light-emitting diodes based on a bipolar transporting luminescent polymer

A soluble electroluminescent polymer containing hole-deficient triphenylamine and electron-deficient oxadiazole units in the main chains has been designed and studied. The design is based on the consideration that the triphenylamine group possesses good hole-transporting property and the oxadiazole unit is known to be of electron-transporting character. Because of the good bipolar transporting performance, the brightness and electroluminescent efficiency are significantly improved and the turn-on voltage is reduced compared with a similar polymer without the electron-deficient oxadiazole units in the main chains. For a device with configuration ITO/PEDOT/polymer/CsF/Al, a maximum brightness of 3600 cd m(-2) and a maximum luminescent efficiency of 0.65 cd A(-1) (quantum efficiency of 0.3%) were obtained in the polymer with oxadiazole units, about 15 times brighter and 15 times more efficient than the corresponding polymer without oxadiazole units.