溶藻细菌DC-L5的分离、鉴定及其溶藻特性

从滇池蓝藻水华集聚区分离获得一株溶藻细菌DC-L5,通过形态及16S rDNA测序分析鉴定为短小芽孢杆菌。用小白鼠进行生物安全实验,小白鼠无中毒症状。研究表明当细菌处于对数生长期时溶藻效果最强,共培养5d使铜锈微囊藻的叶绿素a含量下降83.33%,使惠氏微囊藻、绿色微囊藻、水华束丝藻和水华鱼腥藻4种蓝藻叶绿素a下降率最高为67.6%,最低为58.5%,平均为62.25%。离心沉降后,发现沉淀菌体和无菌上清液对铜锈微囊藻都有溶藻效果,但溶藻效果不及原菌液,推测DC-L5可能是通过直接接触使藻细胞凝聚下沉及进

银鲫卵母细胞特异表达凝集素的抗体制备及其定位分析

银鲫卵母细胞特异的凝集素(Gibel carp oocyte-specific lectin,GOL)是经差减杂交筛选出来的。本研究采用原核表达方法,利用Pinpoint-T载体表达了该凝集素的部分肽段(从22到214个氨基酸),并经纯化用来免疫家兔制备多克隆抗体。采用免疫荧光共定位方法,证明该凝集素是卵母细胞中皮质颗粒的成分之一。

纳米材料对环境和健康的潜在危害

随着越来越多的纳米商品材料在生产和生活中的应用,纳米材料的环境安全问题逐渐引起关注,纳米科技和纳米材料的潜在风险及其负面影响己成为专家共识。文章综述了纳米材料对环境和生命健康带来的可能危害及其毒性特性,并认为深入研究纳米材料的环境安全问题将有助于建立正确生产、使用和处理纳米材料的科学规范。

锥虫系统发育的研究进展

锥虫(trypanosome)是最早在鱼类血液中发现、随后在几乎所有的脊椎动物血液里都有发现的原生动物寄生虫。它主要通过水蛭和吸血昆虫传播,使宿主受到不同程度的危害。为了较全面地了解锥虫系统发育的研究进展,本文综述了国内外有关锥虫系统发育研究的四个方面的内容锥虫的起源(介绍了锥虫起源于脊椎动物和起源于无脊椎动物的两种不同观点);锥虫的系统发育方式(单系发育);锥虫的进化是否与寄主存在协同性及进化史上分歧时间的差异性;目前研究中存在的问题。

武汉东湖沉积物的环境地球化学

武汉东湖沉积物的地球化学过程受到人类活动的较大影响。本文报道该湖沉积物的沉积性状和ＣＮ、Ｐ、Ｓｉ、Ｃａ、Ｍｇ、Ｆｅ、Ａｌ、Ｍｎ、Ｃｕ、Ｐｂ、Ｚｎ、Ｃｄ诸元素在沉积层序中的分布，研究了这些元素的地球化学。通过对东湖污染状况和类型的分析，指出营养物质的积累是导致湖泊生态环境恶化的重要原因，提出可用磷含量作为划分东湖沉积物污染程度的指标的观点。

中国粒鲇属Akysis鱼类二新种

<正> 粒鲇科Akyisidae鱼类是分布在东南亚地区的小型鱼类,包括三个属。其中大粒鲇属Achrochodomchthys Bleeker只产于苏门答腊岛、爪哇岛和加里曼丹岛。狭尾粒鲇属Breitensteinia Steindachner只产于苏门答腊岛和加里曼丹岛。粒鲇属Akysis Bleeker鱼类已记录的有下列8种:Akysis variegatus(Bleeker),1846;Akysis macronemus Bleeker,1860;Akysis pictus Gnther,1883;A

白暨豚上呼吸道的解剖和组织学的研究

白鱀豚上呼吸道具有前庭囊、鼻额囊及前颌囊三对气囊,没有副囊和连接囊。三对囊均右大于左,其中又以前庭囊最为显著。喉由甲状软骨、环状软骨、会厌软骨及一对杓状软骨组成,从喉腔壁的组织学观察发现有室皱襞(假声带)的存在。

黑龙江流域鲤鱼的繁殖、食性和生长

<正> 黑龙江流域各地区的渔获物中,鲤鱼所占的比例是相当大的。在黑龙江本流,每年夏季捕捞的主要对象是鲤鱼。在冬季生产中,鲤鱼的产量在整个鱼产量中亦占优势。著名的达赖湖的鱼产中,占主要成份的也是鲤鱼,冬季渔获物中鲤鱼约占70%左右,在夏季也占50%左右。鲤鱼在这一地区,除了天然捕捞生产的以外,也是一种主要的饲养鱼类。未来黑龙江水利枢纽建成后所出现的新水体中,鲤鱼可能是一种主要的养殖对象。因此,了解它的主要的生态问题和考虑它在水库中的发展方向以及应采取的措施都是必要的。

Gynogenesis and sex determination in large-scale loach Paramisgurnus dabryanus (Sauvage)

Gynogenesis was induced using heterologous sperms in large-scale loach, Paramisgurnus dabryanus (Sauvage), in which a ZW/ZZ sex determination was previously proposed. Three microsatellite loci were used to monitor exclusive maternal inheritance of gynogenetic progenies. The results showed that high percentages of meiogynogens were produced at 4 min post-fertilization and mitogynogens were produced at 18 min post-fertilization by heat shocks, while meiotic gynogenesis was induced by cold shocks within a wide period and high heterozygosity was even observed in gynogens produced at 24 min post-fertilization. The sex ratios of the F, progenies in three gynogenetic families were significantly deviated from 1: 1 expectation with a female bias in two families and a male bias in one family (P < 0.05), and the other four gynogenetic families showed approximate 1:1 sex ratios. Moreover, the self-mating between gynogenetic F, progenies and mating between gynogenetic F, progenies and normal individuals produced all-female progenies or identical proportions of females and males. The data of sex ratios generally confirmed that the sex determination in large-scale loach was determined by the putative ZW/ZZ system, and the possible reasons causing the biased sex ratios are discussed.

Effects of live food and formulated diets on survival, growth and protein content of first-feeding larvae of Plelteobagrus fulvidraco

In recent years, much progress has been made in the rearing of fish larvae fed only artificial diets. A preliminary study was made in an attempt to evaluate the effects of live food and formulated diets on survival, growth and body protein content of first-feeding larvae of Plelteobagrus fulvidraco. Three test diets varying in protein level were formulated: Feed 1 containing 45% protein, Feed 2 with 50% protein and Feed 3 with 55% protein. Larvae fed live food (newly hatched Artemia, unenriched) were the control. The experiment started 3 days post-hatch and lasted for 23 days. At the end of the 23-day trial, survival was best in the control group (65.6%) whereby the final body weight and specific growth rate (SGR) were significantly lower than those in the test feed groups. At the same time, coefficients of variation for SGR and final body weight in the test groups were significantly higher than those in the control. Whole body protein content in all treatments showed a similar tendency during development: significantly higher 3 days post-hatch, then decreasing significantly, and then increasing unstatistically 10 days post-hatch. All results suggest that live food is still better for first-feeding larvae of P. fulvidraco, since live food leads to healthier larvae growth.