131 resultados para 371.334


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Scutellarin was purified from the plant Erigeron breuiscapus (Vant.) Hand.-Mazz. The activity against 3 strains of human immunodeficiency virus (HIV) was determined in vitro in this study. These were laboratory-derived virus (HIV-I-IIIB), drug-resistant virus (HIV-I-74V), and low-passage clinical isolated virus (HIV-1(KM018)). From syncytia inhibition study, the EC50 of scutellarin against HIV-I-IIB direct infection in C8166 cells was 26 mu M with a therapeutic index of 36. When the mode of infection changed from acute infection to cell-to-cell infection, this compound became even more potent and the EC50 reduced to 15 mu M. This suggested that cell fusion might be affected by this compound. By comparing the inhibitory effects on p24 antigen, scutellarin was also found to be active against HIV-1(74V) (EC50 253 mu M) and HIV-1(KM018) (EC50 136 mu M) infection with significant difference in potency. The mechanism of its action was also explored in this study. At a concentration of 433 mu M, scutellarin inhibited 48% of the cell free recombinant HIV-1 RT activity. It also caused 82% inhibition of HIV-1 particle attachment and 45% inhibition of fusion at the concentrations of 54 mu M. In summary, scutellarin was found to inhibit several strains of HIV-1 replication with different potencies. It appeared to inhibit HIV-1 RT activity, HIV-1 particle attachment and cell fusion. These are essential activities for viral transmission and replication. (c) 2005 Elsevier Inc. All rights reserved.

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研究采用PCR-SSCP技术研究番鸭不同就巢群体(就巢1月群、就巢2月群、就巢3月群)、番鸭非就巢群和白改鸭5个群体250个个体PRL基因第4外显子多态性与就巢性状之间的相关性.结果表明,在外显子4编码区内发现2个SNP位点,分别位于该基因的3 777 bp (T/C)和3 785 bp(A/C)处.基因型与就巢性状指标相关分析的结果表明,番鸭非就巢群体与各就巢群体间差异极显著(P<0.01),同时番鸭与白改鸭差异显著(P<0.05),推测PRL基因与就巢性有一定的相关.

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下载PDF阅读器分别于2008年1月和5-6月对位于中国西南边陲的中缅界河--南拉河进行了鱼类资源调查.从野外调查结果及文献资料得知,南拉河共有鱼类42种,隶属4目14科34属.鲤形目的鱼类最多,共有27种,占总种数的64.3%;鲇形目和鲈形目均为6种,占总种数的14.3%;合鳃鱼目2种,占总种数的4.8%.其中,丝尾(鱼蒦)(Hemibagrus wyckioides)被列入<中国物种红色名录>.疑似分布种类--长丝(鱼芒)(Pangasius sanitwangsei)被列入<中国濒危动物红皮节>和<中国物种红色名录>.南拉河鱼类区系是以喜温的"老第三纪类群"中的热带河平原鱼类为主要成分的,为典型的东南亚热带鱼类区系类型;以底栖鱼类和杂食性鱼类居多.导致南拉河鱼类现状的原因主要为水质污染、过度捕捞、外来种的影响.

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在执行全球环境基金(GEF)资助、世界银行管理、中国科学院昆明动物研究所承担的"滇池淡水水生生物多样性恢复项目"中,对滇池东南岸晋宁县新街乡下梁王的湿地生物多样性恢复示范基地进行物种监测时,曾经于2006年6月19日观察到一种较小的鸥类.

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滇金丝猴其主要食物是被子植物, 应更适应热带、亚热带被子植物环境, 而 非高山寒温带云冷杉植物环境。表4参42

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本文记述了蝽科二新种, 分别属于珀蝽属(Plautia Stal)和卷蝽属(Paterculus Distant). 文中描述所用长度单位均为mm。

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