中国牛蛭属一新种记述(吻蛭目,医蛭科)

报道了采自云南省景东县的牛蛭属Poecilobdella Blanchard,18931新种。由于新种总体环数为99环,生殖孔分别在30(Ⅺ)与38(ⅩⅢ)环内,两孔相隔7环;肛门在99环与尾吸盘交界线上;附睾呈卵圆形,精管膨腔呈梨形并且为前者的1/3大小;阴道盲囊呈圆球形,阴道管粗短且无阴道柄。因此与本属中所有的已知种均不相同。

瓣结鱼食性的研究

以采自贵州境内清水江、都柳江的318尾瓣结鱼(Tor(Foliter)brevifilis brevifilis)为研究对象,从食物组成、摄食强度和摄食形态学等方面研究了瓣结鱼食性的特点。结果表明:瓣结鱼的食物由双翅目昆虫幼虫等17类饵料生物组成,并以双翅目昆虫幼虫的出现率最高。瓣结鱼的充塞度按春、夏、秋、冬季逐渐增高,季节性变化明显。充塞指数随体长生长显著减少,而摄食率则随体长生长逐渐增加。充塞指数和摄食率均以V期性腺个体为最低。瓣结鱼为底层杂食性鱼类。

清水江的鱼类区系及生态类型

为了系统研究清水江的鱼类区系和生态类型,在2002—2004年对清水江进行了鱼类标本的采集和生态学调查。共采集鱼类标本2500余号,经鉴定为71种(亚种)。其中中华花鳅、拟尖头鲌、长身鳜和长吻鮠等52种(亚种)鱼类为清水江鱼类新记录,而红尾副鳅、长薄鳅、海南拟、福建纹胸鮡和长须黄颡鱼则首次被发现分布于沅江水系。结合文献记录与调查表明:清水江共有鱼类4目13科57属86种(亚种),其中仅胡子鲇为外来种;清水江鱼类区系具有土著鱼类占优势、特有种类和易(濒)危种类较多、区系组成较复杂和与珠江水系之间存在着鱼类

中国绿球藻目的新种和新变种

饶钦止和沈国华等人于1950，1952，1953，1955和1990年自湖北、江苏、广西和黑龙江等地采得标本，经鉴定表明，它们为中国绿球藻目（Chlorococcales），分隶于6属的8个新种和4个新变种。模式标本保存于中国科学院水生生物研究所藻类标本室（HBI）。

用污染指示菌评价城市水体污染的研究

选择湖南省常德市江北城区水体为代表开展本项研究。结果表明，水体中污染指示菌，总大肠菌群（ＴＣ）和粪大肠菌群（ＦＣ）密度同ＢＯＤ＿５负荷以及综合污染指数Ｐ呈高度正相关。所以，污染指示菌指标，特别是粪大肠菌群指标足综合评价城市污水，尤其是生活污水污染的一个必不可少的重要参数。对该城区水体细菌污染的评价结果是，生活污水排放愈集中的地区，细菌污染愈严重。其中护城河水的ＴＣ密度超出我国地面水Ⅲ级标准４个数量级；ＦＣ密度超过ＷＨＯ娱乐用水标准３个数量级。护城河是细菌污染最严重水体。包括滨湖公园内湖在内的接纳生活污水多

武汉东湖轮虫种类组成及其分类讨论

作者根据Koste(1978)提出的轮虫分类系统对武汉东湖4个站1991年的轮虫种类进行了分类;同时,对我国以往的轮虫分类中存在的若干问题进行了讨论。 1991年作者共观察到轮虫69种,隶属于19个科,29个属。新记录12种。其中,采自水样的轮虫种类有59种,沉积物中休眠卵萌发的种类41种,两者相同种类31种,休眠卵萌发特有种类10种,其中的盘状鞍甲轮虫(Lepadella patella)、侧刺伏嘉轮虫(Wolga spinifera)、侧扁棘管轮虫(Mytilina cortlpressa)等在60年

武汉东湖磷细菌种群结构的研究

对东湖磷细菌种群结构研究结果表明,至少有芽孢杆菌属、微球菌属、不动细菌属、气单胞菌属、产碱杆菌属、贝内克氏菌属、无色杆菌属、短杆菌属、肠杆菌属、黄杆菌属、微杆菌属、假单胞菌属、沙雷氏菌属、黄单胞菌属和发酵单胞菌属15个属的不同菌株以其对有机磷、无机磷化合物的分解能力推动着湖泊中磷的循环。其中以芽孢杆菌属和微球菌属居于明显的优势。磷细菌在湖泊中的分布随水质的污染程度和磷化合物含量的不同而表现有明显的差别:在数量上,湖泥高于湖水,污染较重的Ⅰ站高于水质较好的Ⅱ站,在调查期间,有机磷细菌数在多数情况下亦高于无机

Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp

Previous studies have demonstrated that germinal vesicle of amphibian oocyte contains small nuclear ribonucleoprotein polypeptide C (SNRPC). In this study, a putative member of SNRPC was identified from Carassius auratus gibelio oocyte cDNA library. Its full-length cDNA has an open reading frame of 201 nt for encoding a peptide of 66 an, a short 5'-UTR of 19 nt and a long 3'-UTR of 347 nt including a polyadenylation signal and poly- (A) tail, and the deduced amino acid sequence has 47% identity with the C-terminal of the zebrafish small nuclear ribonucleoprotein polypeptide C. Western blot analysis revealed its oocyte-specific expression. Immunofluorescence localization indicated that its gene product localized to numerous nucleoli within the oocytes and showed dynamic changes with the nucleoli during oocyte maturation. RT-PCR and Western blot analysis further revealed its constant presence in the oocytes and in the embryos until hatching. The data suggested that the newly identified CagOSNRPC might be a nucleolar protein. (c) 2006 Elsevier Inc. All rights reserved.

Growth and energy budget of F-2 'all-fish' growth hormone gene transgenic common carp

The growth and energy budget for F-2 'all-fish' growth hormone gene transgenic common carp Cyprinus carpio of two body sizes were investigated at 29.2 degrees C for 21 days. Specific growth rate, feed intake, feed efficiency, digestibility coefficients of dry matter and protein, gross energy intake (I-E), and the proportion of I-E utilized for heat production (H-E) were significantly higher in the transgenics than in the controls. The proportion of I-E directed to waste products [faecal energy (F-E) and excretory energy loss (Z(E) + U-E) where Z(E) is through the gills and U-E through the kidney], and the proportion of metabolizable energy (M-E) for recovered energy (R-E) were significantly lower in the transgenics than in the controls. The average energy budget equation of transgenic fish was as follows: 100 I-E = 19.3 F-E + 6.0 (Z(E) + U-E) + 45.2 H-E + 29.5 R-E or 100 M-E = 60.5 H-E + 39.5 R-E. The average energy budget equation of the controls was: 100 I-E = 25.2 F-E + 7.4 (Z(E) + U-E) + 35.5 H-E + 31.9 R-E or 100 M-E = 52.7 H-E + 47.3 R-E. These findings indicate that the high growth rate of 'all-fish' transgenic common carp relative to their non-transgenic counterparts was due to their increased feed intake, reduced lose of waste productions and improved feed efficiency. The benefit of the increased energy intake by transgenic fish, however, was diminished by their increased metabolism.

Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei : Cypriniformes)

It is widely accepted that mitochondrial DNA (mtDNA) control region evolves faster than protein encoding genes with few exceptions. In the present study, we sequenced the mitochondrial cytochrome b gene (cyt b) and control region (CR) and compared their rates in 93 specimens representing 67 species of loaches and some related taxa in the Cobitoidea (Order Cypriniformes). The results showed that sequence divergences of the CR were broadly higher than those of the cyt b (about 1.83 times). However, in considering only closely related species, CR sequence evolution was slower than that of cyt b gene (ratio of CR/cyt b is 0.78), a pattern that is found to be very common in Cypriniformes. Combined data of the cyt b and CR were used to estimate the phylogenetic relationship of the Cobitoidea by maximum parsimony, neighbor-joining, and Bayesian methods. With Cyprinus carpio and Danio rerio as outgroups, three analyses identified the same four lineages representing four subfamilies of loaches, with Botiinae on the basal-most clade. The phylogenctic relationship of the Cobitoidea was ((Catostomidae + Gyrinocheilidae) + (Botiinae + (Balitorinae + (Cobitinae + Nemacheilinae)))), which indicated that Sawada's Cobitidae (including Cobitinae and Botiinae) was not monophyletic. Our molecular phylogenetic analyses are in very close agreement with the phylogenetic results based on the morphological data proposed by Nalbant and Bianco, wherein these four subfamilies were elevated to the family level as Botiidae, Balitoridae, Cobitidae, and Nemacheilidae. (c) 2005 Elsevier Inc. All rights reserved.

Using epilithic diatom communities to assess ecological condition of Xiangxi River system

Assessment method for ecological condition of Xiangxi River system was studied by using 13 candidate metrics of epilithic diatom which can reflect conditions in pH, salinity, nitrogen uptake metabolism, oxygen requirements, saprobity, trophic state, morphological character and pollution tolerant capability etc. By one-way ANOVA, the metrics of relative abundance of acidobiontic algae (ACID), freshwater algae (FRESH), high oxygen requirement (HIGH-O), eutraphentic state (EUTRA) and mobile taxa ( MOBILE) were suitable for distinguishing sites in different conditions. Then, the river diatom index (RDI) composed of these five metrics was used to evaluate ecological condition of the river. The results showed that the healthiest sites were in the Guanmenshan Natural Reserve ( with the mean RDI of 79.73). The sites located in tributary of Jiuchong River also owned excellent state (mean RDI of 78.25). Mean RDI of another tributary - Gufu River and the main river were 70.85 and 68.45 respectively, and the unhealthiest tributary was Gaolan River (with mean RDI of 65.64). The mean RDI for all the 51 sites was 71.40. The competence of RDI was discussed with comparison of evaluation results of DAIpo and TDI, it can be concluded that multimetrics is more competent in assessment task.

Moisture content in Dall's porpoise (Phocoenoides dalli) tissues: a reference base for conversion factors between dry and wet weight trace element concentrations in cetaceans

Concentration of trace elements measured by dry weight basis has become more commonly used in recent studies on cetaceans than wet weight basis, which was used more in earlier studies. Because few authors present moisture content data in their papers, it is difficult to compare the concentrations of trace elements between various studies. Therefore, we felt that it would be useful if a reference conversion factor (CF) for tissue types could be found to convert between wet weight and dry weight data on trace element concentrations. We determined the moisture contents of 14 tissues of Dall's porpoise (Phocoenoides dalli), and then, calculated the CF values for those tissues. Because the moisture content of each tissue differs from other tissues, it is necessary to use a specific CIF for each tissue rather than a general CF for several tissues. We have also found that CIF values for Dall's porpoise tissues are similar to the same tissues in other cetaceans. Therefore CF values from Dall's porpoise can be reliably used to convert between wet and dry weight concentrations for other cetacean tissues as reference data. (C) 2002 Elsevier Science Ltd. All rights reserved.

Lineshape Analysis of the Beat Signal Between Optical Carrier and Delayed Sidebands

This paper presents the lineshape analysis of the beat signal between the optical carrier and the shifted and delayed side-bands produced by sinusoidal amplitude modulation. It is shown that the beat signal has a typical lineshape with a very narrow delta-peak superposed on a quasi-Lorentzian profile. Theoretical explanation for the appearance of this peak has been given based on optical spectral structure constructed by a large number of optical wave trains. It is predicted that the delta-peak is originated from the beat between the wave trains in the carrier and those in the delayed sidebands when their average coherence length is longer than the delay line. Experiments carried out using different delay lines clearly show that the delta-peak is always located at the modulation frequency and decreases with the increasing delay line. Our analysis explicitly indicates that the linewidth is related to the observation time. It is also suggested that the disappearance of the delta-peak can be used as the criterion of coherence elimination.

The effects of nonlinear couplings and external magnetic field on the thermal entanglement in a two-spin-qutrit system

We investigate the effects of nonlinear couplings and external magnetic field on the thermal entanglement in a two-spin-qutrit system by applying the concept of negativity. It is found that the nonlinear couplings favor the thermal entanglement creating. Only when the nonlinear couplings vertical bar K vertical bar are larger than a certain critical value does the entanglement exist. The dependence of the thermal entanglement in this system on the magnetic field and temperature is also presented. The critical magnetic field increases with the increasing nonlinear couplings constant vertical bar K vertical bar. And for a fixed nonlinear couplings constant, the critical temperature is independent of the magnetic field B. (c) 2005 Elsevier B.V. All rights reserved.