中华硬蜱凝血酶抑制剂的分离纯化与活性

通过凝胶过滤和反相高压液相层析, 从200 只半饱吸血的中华硬蜱( Ixodide sinesis ) 唾液腺中分离 纯化得到一凝血酶抑制剂。用飞行质谱测定其分子量为61356 kDa , 与其他蜱类来源的凝血酶抑制剂分子量不 同, 提示为一新蛋白分子。该抑制剂对凝血酶有强烈的抑制活性, 对激活的第X 因子和胰蛋白酶有微弱的抑制 活性。其发现将为发展疫苗、生物控制中华硬蜱提供资料和目标抗原。

Phylogenetic relationships of 12 Penaeoidea shrimp species deduced from mitochondrial DNA sequences

DNA sequences of an 847 bp fragment of mitochondrial cytochrome oxidase subunit I (COI) gene and a 514 bp fragment of 16s rRNA gene were determined to examine the phylogenetic relationships of 12 Penaeoidea shrimp species (Penaeus chinensis, Penaeus japon

人类线粒体DNA变异的检测方法和思路

基于线粒体DNA(mtDNA)的研究对于人群源流迁移、线粒体相关疾病病因的探讨和法医鉴定等具有重要意义,就检测人线粒体突变的一些常用方法,如RFLP、SSO和控制区测序等作一小结和归纳,并重点介绍目前mtDNA突变的筛选方法和思路。另外,还总结了近年来对人mtDNA方面的研究结果,对世界人群中主要单倍型类群(haplogroup)特征变异位点和相应的酶切检测引物作了归纳。

Enhanced apoptotic action of trichosanthin in HIV-1 infected cells

Trichosanthin (TCS) is a type 1 ribosome-inactivating protein (RIP) effective against HIV-1 replication. The mechanism is not clear. Present results suggested that the antiviral action tray be partly mediated through enhanced apoptosis on infected cells. TCS induced apoptosis in normal H9 cells and this action was more potent in those infected with HIV-1. In flow cytometry study, TCS induced larger population of apoptotic H9 cells chronically infected with HIV-1 in a dose-dependent manner. At TCS concentration of 25 mu g/ml. 8.4% of normal H9 cells were found to be apoptotic whereas the same concentration induced 24.5% in HIV-1 chronically infected cells. Such difference was not found in the control experiments without TCS treatment. Two other studies supported this action. Cytotoxic study showed that cell viability was always lower in HIV-1 infected cells after TCS treatment, and DNA fragmentation studs confirmed more laddering in infected cells. The mechanism of TCS induced apoptosis in normal or infected H9 cells is not clear. Results in this study demonstrated that TCS is snore effective in inducing apoptosis in HIV-1 infected cells. This may explain in part the antiviral action of TCS. (c) 2005 Elsevier Inc. All rights reserved.

鸭催乳素基因外显子4单核苷酸多态性与就巢性状的相关分析

研究采用PCR-SSCP技术研究番鸭不同就巢群体(就巢1月群、就巢2月群、就巢3月群)、番鸭非就巢群和白改鸭5个群体250个个体PRL基因第4外显子多态性与就巢性状之间的相关性.结果表明,在外显子4编码区内发现2个SNP位点,分别位于该基因的3 777 bp (T/C)和3 785 bp(A/C)处.基因型与就巢性状指标相关分析的结果表明,番鸭非就巢群体与各就巢群体间差异极显著(P<0.01),同时番鸭与白改鸭差异显著(P<0.05),推测PRL基因与就巢性有一定的相关.

Differential amplitude modulation of auditory evoked cortical potentials associated with brain state in the freely moving rhesus monkey

We simultaneously recorded auditory evoked potentials (AEP) from the temporal cortex (TCx), the dorsolateral prefrontal cortex (dPFCx) and the parietal cortex (PCx) in the freely moving rhesus monkey to investigate state-dependent changes of the AEP. AEPs obtained during passive wakefulness, active wakefulness (AW), slow wave sleep and rapid-eye-movement sleep (REM) were compared. Results showed that AEP from all three cerebral areas were modulated by brain states. However, the amplitude of AEP from dPFCx and PCx significantly appeared greater attenuation than that from the TCx during AW and REM. These results indicate that the modulation of brain state on AEP from all three cerebral areas investigated is not uniform, which suggests that different cerebral areas have differential functional contributions during sleep-wake cycle. (C) 2002 Elsevier Science Ireland Ltd.. All rights reserved.

Serum-free culture of rhesus monkey embryonic stem cells

Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF-alpha, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications.

云南滇池濒危特有种滇池金线鲃人工繁殖初报

滇池金线鲃(Sinocyclocheilus grahami)是滇池湖泊生态系统的指示物种和特有种.因水质污染和外来物种入侵等因素的影响,该鱼类自1986年起就从湖体中消失,仅有湖周围的少数龙潭中尚保存有少量滇池金线鲃.至2007年3月初,有两次繁殖试验取得了成功.先后试验了5尾雌鱼和6尾雄鱼,获得鱼卵约1 600粒,并实施干法受精.约有1 320粒受精,平均受精率为73%.孵化出鱼苗约480尾,平均孵化率为36%.经10天饲养,约有95%以上的鱼苗存活,体长达8-12 mm.滇池金线鲃人工繁殖成功的意义有三点:能有效保护该物种免于灭绝;繁殖的鱼苗放归湖泊合适水域,将有助于恢复滇池的土著生物多样性;有助于推动当地水产养殖业从非土著鱼类养殖向土著鱼类养殖的方向转变.

紫茎泽兰精油各馏份对4种仓库害虫的杀虫活性

：用紫撬泽兰精油的20℃～150。C，150℃一200℃，20．0℃，230℃．230屯。300℃和300℃以上馏协，分剐以 稚．簦嘴，L的熏蒸浓爰楚理米象、玉采象、簿豆象牵蚕熹象，螬幕表磷凄5争犍静中，娃15。℃～200℃馏傍转荣虫活性 最高，其洗是20℃一150℃和200℃～230气馏份，而300℃以上馏份的杀虫活性靛低。进一步的生测结装表明．150屯一 200℃增傍对米象、玉来氧、绿黛象和善豆象24h的l(葡分莉为15 5li4，17．8124，培．0302和16．2671mg／Lo

尖吻蝮(Agkistrodon acutus)蛇毒凝血酶样成分的研究Ⅲ:蛇毒凝血酶止血效应的研究

将纯化的蛇毒凝血酶(TLE_(3)、TLE_(4)), 经家免试验表明, 2—3凝血酶单位/kg体 重剂量能显著地使家兔全血凝血时间短1/3—1/2。药后1h即有促凝作用, 以2—4h凝血(止血)效应最强, 12h已消失。经家兔及家犬实验性创伤止血实验表明, 对创伤出血有止血作用。表2参11

特殊涡鞭毛虫---尖尾虫染色体与典型涡鞭毛虫染色体的比较研究

尖尾虫与典型涡鞭毛虫在一系列重要的细胞生物学与分子生物学特性方面有着重大差异。尖尾虫的染色体不仅在亚显微构造上迥然不同于一般的涡鞭毛虫类的染色体, 而且其精细形象并不会因为固定方法的不同而有明显的改变。因此, 建议在原生生物中建立一个新的门---尖尾虫门(Phylum oxyrrhinea)。