Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys

The energy substrates lactate, pyruvate, and glucose were evaluated for supporting in vitro cytoplasmic maturation of rhesus monkey oocytes. A total of 321 cumulus-oocyte complexes (COCs) aspirated from greater than or equal to 1000 mum diameter follicles

Pontibacter akesuensis sp nov., isolated from a desert soil in China

A Gram-negative, non-motile, rod-shaped bacterium, designated strain AKS 1 T, was isolated from a desert soil sample collected from Alkesu, Xin.lang Province, China. A taxonomic study, including phylogenetic analysis based on 16S rRNA gene sequences and p

人类线粒体DNA变异的检测方法和思路

基于线粒体DNA(mtDNA)的研究对于人群源流迁移、线粒体相关疾病病因的探讨和法医鉴定等具有重要意义,就检测人线粒体突变的一些常用方法,如RFLP、SSO和控制区测序等作一小结和归纳,并重点介绍目前mtDNA突变的筛选方法和思路。另外,还总结了近年来对人mtDNA方面的研究结果,对世界人群中主要单倍型类群(haplogroup)特征变异位点和相应的酶切检测引物作了归纳。

The Acquisition of an Inheritable 50-bp Deletion in the Human mtDNA Control Region Does Not Affect the mtDNA Copy Number in Peripheral Blood Cells

The mitochondrial DNA (mtDNA) control region is believed to play an important biological role in mtDNA replication. Large deletions in this region are rarely found, but when they do occur they might be expected to interfere with the replication of the molecule, thus leading to a reduction of mtDNA copy number. During a survey for mtDNA sequence variations in 5,559 individuals from the general Chinese population and 2,538 individuals with medical disorders, we identified a 50-bp deletion (m.298_347del50) in the mtDNA control region in a member of a healthy Han Chinese family belonging to haplogroup B4c1b2, as suggested by complete mtDNA genome sequencing. This deletion removes the conserved sequence block II (CSBII; region 299-315) and the replication primer location (region 317-321). However, quantification of the mtDNA copy number in this subject showed a value within a range that was observed in 20 healthy subjects without the deletion. The deletion was detected in the hair samples of the maternal relatives of the subject and exhibited variable heteroplasmy. Our current observation, together with a recent report for a benign 154-bp deletion in the mtDNA control region, suggests that the control of mtDNA replication may be more complex than we had thought. Hum Mutat 31:538-543, 2010. (C) 2010 Wiley-Liss, Inc.

天然来源具有抗HIV 活性的多肽和蛋白类化合物

　从天然药物中寻找高效低毒的抗HIV/ AIDS 活性化合物和先导化合物是国内外新药研制中非 常活跃的领域之一,也应该是我国近期创制新药的一条捷径。来源于动植物、海洋生物、微生物以及人体等中 的许多天然化合物具有抗HIV 活性。本文仅对天然来源的多肽和蛋白类化合物抗HIV 的研究简要综述。

广西云南鳅属鱼类一新种记述

2003年5月,在广西壮族自治区都安县高岭乡红水河水系采集到一批条鳅标本.经鉴定,为云南鳅属(Yunnanilius)一未经发表的新种.新种长须云南鳅(Yunnanilus longibarbatus sp. nov.) 属于黑斑云南鳅种组(Yunnanilus nigromaculatus group),并与黑斑云南鳅[Y.nigromaculatus (Regan)]、钝吻云南鳅(Y.obtusirostris Yang)、长背云南鳅(Y.longidorsalis Li, Tao et Lu)和巴江云南鳅(Y.bajingensis Li)等4种最为相似.长背云南鳅背鳍分枝鳍条11根,颌须短,仅达眼中部,背鳍起点距吻端较距尾鳍基为近,可与包括本种在内的其余4种相区别(背鳍分枝鳍条8-9根,颌须达到或超过眼后缘,背鳍起点距吻端较距尾鳍基为远).本种和钝吻云南鳅上颌中央无齿状突、下颌中央无缺刻,可与黑斑云南鳅和巴江云南鳅相区别.本种在鳍条数、上下颌、背鳍起点的位置、尾鳍形状、鳞片、头部侧线管孔、体侧纵纹等方面与钝吻云南鳅最为相似,但本种可藉多个特征与后者相区别:颌须伸过眼后缘至鳃盖后缘的中点至鳃盖后缘,是已知云南鳅中须最长的;背鳍不分枝鳍条下1/3处具一不显著黑色斑点 (vs. 无斑);体侧纵纹,其宽窄于眼径,较大个体背鳍之前的纵纹被虫状纹取代 (vs. 体侧纵纹始终存在,其宽约等于眼径);头背具斑点 (vs. 无斑);尾柄较细长,体长/尾柄长=5.3-6.1 (vs. 7.4-9.0,尾柄长/尾柄高=1.3-1.8 vs. 0.8-1.1).

昆明果蝇属一新种(双翅目: 果蝇科)

新种长鬃果蝇D. (D.)longisetae属伊米果蝇种团。与D.(D.)chamundiensis的区别在于第9背板上长鬃着生位置、触角芒分枝毛数及核型。图6参2

金不换鲜三七液对哺乳动物致突变和致畸安全性评价

以小鼠骨髓细胞染色体畸变试验、小鼠睾丸减数分裂染色体畸变及小鼠致畸试验为指标,研究金不换鲜三七液的安全性。结果:小鼠骨髓细胞染色体畸变试验;低、中、高3个剂量组小鼠骨髓细胞染色体畸变率分别为0.7%、0.2%和0.9%,与对照组相比无显著差异。阳性对照组染色体畸变率大大增高。小鼠睾丸减数分裂细胞染色体畸变:在该实验条件下,小鼠睾丸细胞染色体畸变率[包括性染色体单价体(X-YU),常染色体单价体(AU)]在各实验组和对照组之间无显著差异。小鼠致畸试验:小鼠口服金不换鲜三七液的累积剂量达15g/kg体重的1/10、1/5和1/2,小鼠致畸试验也无显著差异。