饲养条件下白豚微量元素的积累

中国科学院水生生物研究所留学归国人员基金资助项目(020102); 中国水产科学研究院首席科学家基金资助项目(1-105011)

青海湖裸鲤繁殖群体遗传多样性的RAPD分析

采用随机扩增多态性DNA(RAPD)方法对青海湖裸鲤的3个洄游繁殖群体-黑马河(HM)、布哈河(BH)及沙柳河(SL)群体各30个个体的DNA多态性进行了分析。用13个引物在三个群体中共检测出85个位点,其中多态性位点68个。青海湖裸鲤群体总的DNA多态位点百分率为80.0%。数据分析结果显示:青海湖裸鲤3个群体总的Nei基因多样性为0.3395,Shannon遗传多样性信息指数为0.4861,存在着较为丰富的遗传多样性。青海湖裸鲤3个群体间平均遗传距离为0.0788,基因分化系数Gst为0.1070,表

多甲藻对不同光照强度的反应:色素沉着及暗光适应的可能性(英文)

在ＨＰＬＣ色素分析的基础上，研究了多甲藻对不同光照强度（１６．６—２５０μ·Ｅｍ－２ｓ－１） 的反应。类胡萝卡素中最重要的多甲藻素和硅甲藻黄素占叶黄素总量的９０％。随光照强度的 逐步增加，多甲藻细胞中叶绿素ａ含量呈现明显的下降趋势。结果表明硅甲藻黄素和β胡萝卜 素具有光保护作用：１）当多甲藻暴露于较强光下时，硅甲藻黄素／叶绿素ａ之比呈现出明显增 加的趋势。β胡萝卜素／叶绿素ａ之比也显现出明显的增加趋势。２）β胡萝卜素／叶绿素ａ之比 在有光条件下比在完全黑暗条件下要高；３）硅甲藻黄素／叶绿素ａ之比在高辐照度

在不同水硬度下铜对草鱼、鲢和大鳞泥鳅的急性毒性

实验以草鱼、鲢和大鳞泥鳅的鱼苗为材料,测定了水硬度对铜的急性毒性的影响,水硬度在255、141、37和14mg/L(以 CaCO_3计)时铜对草鱼的48小时 LC_(50)分别是519、354、91和76μg/L,96小时 LC_(50)则分别为460、307、67和39μg/L.在同样的硬度条件下,对鲢的48小时 LC_(50)是452、393、71和40μg/L,96小时 LC_(50)分别为452、296、41和31μg/L.大鲢泥鳅鱼苗在硬度260、137、36和10μg/L(以 CaCO_3计)

青鱼池和鲩鱼池中浮游生物的组成及其与混养白鲢生长的关系

<正> 在进行“养鱼池单位面积产量试验”的同时,曾经每月二次采集了浮游生物的标本。这些材料,现在将依照浮游生物的种类、组成以及季节变化三方面加以分析和总结。目的在于说明:照鱼农的一般养法,青鱼池在经常投入动物性饲料和鲩鱼池经常投入植物性饲料的情形下,在浮游生物的组成上各表现出什么特点;二者互相比较,又有什么异同。

Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper

Aromatase plays a key role in sex differentiation of gonads. In this study, we cloned the full-length cDNA of ovarian aromatase from protogynous hermaphrodite red-spotted grouper (Epinephelus akaara), and prepared the corresponding anti-EaCyp19a1a antiserum. Western blot and immunofluorescence studies revealed ovary-specific expression pattern of EaCyp19a1a in adults and its dynamic expression change during artificial sex reversal. EaCyp19a1a was expressed by follicular cells of follicular layer around oocytes because strong EaCyp19a1a immunofluorescence was observed in the cells of ovaries. During artificial sex reversal, EaCyp19a1a expression dropped significantly from female to male, and almost no any positive EaCyp19a1a signal was observed in testicular tissues. Then, we cloned and sequenced a total of 1967 bp T-flanking sequence of EaCyp19a1a promoter, and showed a number of potential binding sites for some transcriptional factors, such as SOX5, GATA gene family, CREB, AP1, FOXL1, C/EBP, ARE and SF-1. Moreover, we prepared a series of 5' deletion promoter constructs and performed in vitro luciferase assays of EaCyp19a1a promoter activities. The data indicated that the CREB regulation region from -1010 to -898 might be a major cis-acting element to EaCyp19a1a promoter, whereas the elements GATA and SOX5 in the region from -1216 to -1010 might be suppression elements. Significantly, we found a common conserved sequence region in the fish ovary-type aromatase promoters with identities from 93% to 34%. And, the motifs of TATA box, SF-1, SOX5, and CREB existed in the region and were conserved among the most of fish species. (C) 2009 Elsevier Ireland Ltd. All rights reserved.