D77, one benzoic acid derivative, functions as a novel anti-HIV-1 inhibitor targeting the interaction between integrase and cellular LEDGF/p75

Integration of viral-DNA into host chromosome mediated by the viral protein HIV-1 integrase (IN) is an essential step in the HIV-1 life cycle. In this process, Lens epithelium-derived growth factor (LEDGF/p75) is discovered to function as a cellular co-fa

Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender

Aim: To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. Methods: Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris (mTTE) synthetic extender and glycerol as cryoprotectant. The effects of glycerol concentration (1%,3%, 5%, 10% and 15% [v/v]) and its equilibration time (10 min, 30 min, 60 min and 90 min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity. Results: The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P < 0.05) for 5% glycerol (42.95 +/- 2.55 and 50.39 +/- 2.42, respectively) than those of the other groups (1%: 19.19 +/- 3.22 and 24.84 +/- 3.64; 3%: 34.23 +/- 3.43 and 41.37 +/- 3.42; 10%: 15.68 +/- 2.36 and 21.39 +/- 3.14; 15%: 7.47 +/- 1.44 and 12.90 +/- 2.18). The parameters for 30 min equilibration (42.95 2.55 and 50.39 2.42) were better (P < 0.05) than those of the other groups (10 min: 31.33 +/- 3.06 and 38. 98 +/- 3.31; 60 min: 32.49 +/- 3.86 and 40.01 +/- 4.18; 90 min: 31.16 +/- 3.66 and 38.30 +/- 3.78). Five percent glycerol and 30 min equilibration yielded the highest post-thaw sperm motility and head membrane integrity. Conclusion: Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender, which is related to the concentration and the equilibration time of glycerol.

常用鱼药漂白粉和氯杀宁对背角无齿蚌的毒性影响

试验以洱海流域的背角无齿蚌为研究对象,确定常用鱼药:漂白粉、氯杀宁对湖泊大型底柄动物背角无齿蚌的毒性影响.试验得出漂白粉作用下,背角无齿蚌24 h、48 h、72 h、96 h、120 h、144 h、168 h的半致死率;氯杀宁作用下,背角无齿蚌24 h、48 h、72 h、96 h的半致死率;漂白粉有效氯安全浓度95.25～108.86 mg/L;氯杀宁有效氯安全浓度3.32～3.55 mg/L.通过显微镜观察,漂白粉和氯杀宁的主要作用部位为背角无齿蚌的呼吸系统,在其鳃上可见明显病变.

Opiate withdrawal modifies synaptic plasticity in subicular-nucleus accumbens pathway in vivo

Subiculum receives output of hippocampal CAI neurons and projects glutamatergic synapses onto nucleus accumbens (NAc), the subicular-NAc pathway linking memory and reward system. It is unknown whether morphine withdrawal influences synaptic plasticity in

丽江拉市海保护区的鱼类区系和现存状态

2001年8、10月和1999年10月在云南丽江拉市海高原湿地自然保护区采得鱼类标本4550号. 结合文献记录, 并用参与式农村评估(PRA)与渔民讨论, 评估各鱼种的生存状态. 初步确定该保护区共有鱼类25种, 隶属5目10科21属. 其中有7个土著种, 18个引入种, 高度濒危或灭绝3种. 首次记录到秀丽高原鳅. 该地鱼类区系主要由引入种组成, 土著种正在消失。

猕猴结肠小袋纤毛虫(Balantidium coli)在猴群中感染情况的调查

笼养猕猴250只, 有结肠小袋纤毛虫寄生者91只, 检得虫体2365个,测量了144 个原虫的长度和宽度, 并观察了它们的外形, 分析了猴的感染情况, 其中仔猴感 染率占42.86%, 病态猴42.85%, 检疫猴群中有本原虫感染的占42.5%。长期饲养 的猴群感染率为24.42% 。年龄在5岁以上者感染率低。栖息笼舍的构造和卫生 管理工作对感染率影响较大。仔猴的 感染与亲代阳性猴有关, 一年四季均可感 染, 以冬季为高。图1表2参11

景东无量山和哀牢山黑长臂猿考察报告

1987年到1989年中三次调查发现, 无量山有20群长臂猿, 平均群密度为0.62 群/km~(2), 估计保护区约有144群。哀牢山徐家坝地区有5群, 大部分猿群在 海拔2100—2500m之间。就目前而言, 景东无量山和哀牢山地区是我国保护黑长 臂猿的最重要基地。图1表1

双齿果蝇物种群Lordiphosa denticeps group的分类地位及四新种记述(双翅目: 果蝇科)

根据雄性前足跗节具似性梳状的鬃、肛板下缘具粗黑色齿、抱器具方向向上的簇鬃、后阳基侧突屋脊状、导卵器有许多散生的盘齿等双齿果蝇物种群的特有的特性, 认为双齿果蝇物种群有效, 应作为拱背果蝇属独立的物种群。并记述了4新种: 刀形拱背果蝇L. cultrata, 德钦拱背果蝇L. deqenensis, 双叉拱背果蝇L. ramula和拟双叉拱背果蝇L. falsiramula。连同已知种列成检索表以资区别。模式标本存作者单位。图15参7

垂体腺苷酸环化酶激活肽的研究概况

垂体腺苷酸环化酶激活肽(PACAP)及其受体存在于许多动物的下丘脑和垂体中,而且在肾上腺、睾丸、卵巢、肝脏、肾脏、胰腺、松果腺、心脏、脊椎、神经节、呼吸系统和消化系统等组织或系统中也存在,其中肾上腺含量最高。在这些组织或系统中,通过Ca~(2+)、Na~(+)、腺苷酸环化酶或磷酸肌醇等作用通路,PACAP发挥神经递质/调质、或神经营养因子等生物学功能。

Pituitary adenylate cyclase-activating polypeptide

Pituitary adenylate cyclase-activating polypeptide (PACAP) which belongs to the secretin/glucagon/ VIP family has been originally isolated from the sheep hypothalamus on the basis of its ability to stimulate cAMP formation in culture rat anterior pituitary cells. Post-translational processing of the PACAP precursor generates two biologically active molecular forms, PACAP-38 and PACAP-27. The primary structure of PACAP has been remarkably conserved during evolution. The sequence of PACAP-27 exhibits substantial similarities with those of vasoactive intestinal polypeptide (VIP), glucagon and secretin. The gene encoding the PACAP precursor is widely expressed in brain and various peripheral organs, notably in endocrine glands, gastro-intestinal, urogenital tracts and respiratory system. In vivo, and in vitro studies have shown that PACAP exhibits multiple activities especially a trophic activity during ontogenesis, notably in the adrenal medulla and the central nervous system. The biological effects of PACAP are mediated through three distinct receptor subtypes which exhibit differential affinities for PACAP and VIP. The PAC1 receptor, which shows high selectivity for PACAP, is coupled to several transduction systems. In contrast, VPAC1 and VPAC2, which bind with the same affinity for PACAP and VIP, are mainly coupled to the adenylyl cyclase pathway. In conclusion, PACAP is neuropeptide, and it functions as a hypothalamic hormone, neurohormone, neuromodulator, vasodilator, neurotransmitter or trophic factor in the brain and the various organs.