大蹼铃蟾蛋白提取物对膀胱癌细胞株的抑制作用及对端粒酶的影响

目的探讨大蹼铃蟾蛋白提取物对膀胱癌细胞株的 抑制作用及对端粒酶的影响。方法采用肿瘤细胞培养方 法，检测大蹼铃蟾蛋白提取物对膀胱癌细胞株."%、/01’)+、 234/56的抑制作用；以.&-7’78&’9:02-方法检测大蹼铃 蟾蛋白提取物对膀胱癌细胞株端粒酶表达的影响。结果 大蹼铃蟾蛋白提取物表现出对膀胱癌细胞株有较强的抑制 作用；对膀胱癌细胞株端粒酶表达则无影响。结论大蹼铃 蟾蛋白提取物对多个膀胱癌细胞株具有直接细胞杀伤作用， 其作用机制不通过抑制端粒酶活性。

无指盘臭蛙抑生肽、其基因和变异体及其在制药中的应用

本发明涉及无指盘臭蛙抑生肽、其基因和变异体及其在制药中的应用，属生物医学技术领域。无指盘臭蛙抑生肽是一种多肽，分子量2080.64道尔顿，等电点9.39，具有病原微生物抑制活性。无指盘臭蛙抑生肽全序列为：NH2-GIFGKILGVGKKVLCGLSGMC-COOH。编码的基因由304个核苷酸组成，其中编码成熟部分的为第136-198位核苷酸。改变无指盘臭蛙抑生肽原始序列中一个或几个氨基酸所产生的变异体，人工合成的无指盘臭蛙抑生肽及其变异体具有强烈的病原微生物抑制活性，以及肿瘤抑制活性。而变异体在这些抑制作用中表现出经改良的特性。无指盘臭蛙抑生肽及其变异体作为制备治疗肿瘤和病原微生物感染疾病的治疗药物的应用。并且还具有序列简单、合成方便活性强等优点。

The role of MC1R gene in buffalo coat color

Melanocortin-1 receptor (MC1R) plays a major role in pigmentation in many species. To investigate if the MC1R gene is associated with coat color in water buffalo, the coding region of MC1R gene of 216 buffalo samples was sequenced, which included 49 black river buffalo (Murrah and Nili-Ravi), 136 swamp buffalo (Dehong, Diandongnan, Dechang, Guizhou, and Xilin) with white and gray body, and 31 hybrid offspring of river buffalo Nili-Ravi (or Murrah) and swamp buffalo. Among the three variation sites found, SNP684 was synonymous, while SNP310 and SNP384 were nonsynonymous, leading to p.S104G and p.I128M changes, respectively. Only Individuals carrying homozygote E-BR/E-BR were black. The genotype and phenotype analysis of the hybrid offspring of black river buffalo and gray swamp buffalo further revealed that the river buffalo type allele E-BR or the allele carrying the amino acid p.104S was important for the full function of MC1R. The in silico functional analysis showed that the amino acid substitutions p.G104S and p.M128I had significant impact on the function of MC1R. Above results indicate that the allele E-BR or the allele carrying the amino acid p.104S was associated with the black coat color in buffalo.

Polymerase chain reaction based C4AQ0 and C4BQ0 genotyping: association with systemic lupus erythematosus in southwest Han Chinese

Objective: To investigate the association of complement C4 null genes (C4QO, including C4AQO and C4BQO) and C2 gene with systemic lupus erythematosus (SLE) in southwest Han Chinese; 136 patients with SLE and 174 matched controls were genotyped. Methods: C4 null genes were determined by a polymerase chain reaction (PCR) procedure with sequence specific primers (PCR-SSP). The 2 bp insertion in exon 29, which was previously identified in non-Chinese populations and caused defective C4A genes, was directly typed by sequencing the whole exon 29 using exon specific primers. The exon 6 of complement C2 was also sequenced in both the patients and controls. Results: The frequency of homozygous C4AQO allele was 12.5% (17/136) in patients with SLE compared with 1.1% (2/174) in controls (p<0.001, odds ratio (OR)=12.286, 95% confidence interval (95% CI) 2.786 to 54.170). There was no significant difference for homozygous C4BQO allele between patients with SLE and controls (p=0.699). Patients with the C4AQO gene had an increased risk of acquiring renal disorder, serositis, and anti-dsDNA antibodies compared with those without C4AQO (for renal disorder, p=0.018, OR=8.951, 95% Cl 1.132 to 70.804; for serositis, p=0.011, OR 4.891, 95% CI 1.574 to 15.198; for anti-dsDNA, p=0.004, OR 7.630, 95%Cl 1.636 to 35.584). None of the patients or controls had the 2 bp insertion in exon 29 of the C4 gene. The type I C2 deficiency was not detected in the 3 10 samples. Conclusion: It is suggested that deficiency of C4A (not due to a 2 bp insertion in exon 29), but not C4B or C2, may be a risk factor for acquiring SLE in south west Han Chinese; this results in increased risk of renal disorder, serositis, and anti-dsDNA antibodies in patients with SLE. Racial differences seem to be relevant in susceptibility to SLE.

HIV-1核衣壳蛋白NCp7抑制剂体外筛选方法的建立

目的 表达HIV-1核衣壳蛋白(nucleocapside protein p7,NCp7),并建立抑制剂筛选方法.方法 从pBRU2/ADP202质粒经PCR得到HIV-1 NCp7编码序列,克隆到pet28a 质粒中构建HIV-1 NCp7表达载体.阳性克隆转染E.coli BL21 DE3,以IPTG诱导,细胞裂解液经Q Sepharose H.P和SP Sepharose H.P纯化后纯度达到90%以上.将具有锌离子逐出活性的H2O2和2,2'-联硫基二吡啶(2,2'-dithiodipyridine,AT-2)与NCp7作用,用锌离子特异的荧光染料检测.用该方法检测KIZ-CM系列化合物.结果 可以检测H2O2和AT-2的锌离子逐出活性,经该方法筛选,KIZ-CM 10,18,19等化合物具有一定的锌离子逐出活性.结论 在国内首次建立了HIV-1 NCp7锌离子逐出方法,该方法可以用于NCp7抑制剂的筛选.

The anti-HIV-1 effect of scutellarin

Scutellarin was purified from the plant Erigeron breuiscapus (Vant.) Hand.-Mazz. The activity against 3 strains of human immunodeficiency virus (HIV) was determined in vitro in this study. These were laboratory-derived virus (HIV-I-IIIB), drug-resistant virus (HIV-I-74V), and low-passage clinical isolated virus (HIV-1(KM018)). From syncytia inhibition study, the EC50 of scutellarin against HIV-I-IIB direct infection in C8166 cells was 26 mu M with a therapeutic index of 36. When the mode of infection changed from acute infection to cell-to-cell infection, this compound became even more potent and the EC50 reduced to 15 mu M. This suggested that cell fusion might be affected by this compound. By comparing the inhibitory effects on p24 antigen, scutellarin was also found to be active against HIV-1(74V) (EC50 253 mu M) and HIV-1(KM018) (EC50 136 mu M) infection with significant difference in potency. The mechanism of its action was also explored in this study. At a concentration of 433 mu M, scutellarin inhibited 48% of the cell free recombinant HIV-1 RT activity. It also caused 82% inhibition of HIV-1 particle attachment and 45% inhibition of fusion at the concentrations of 54 mu M. In summary, scutellarin was found to inhibit several strains of HIV-1 replication with different potencies. It appeared to inhibit HIV-1 RT activity, HIV-1 particle attachment and cell fusion. These are essential activities for viral transmission and replication. (c) 2005 Elsevier Inc. All rights reserved.

中国灵猫科的支序系统学分析

选取分布于中国境内的8属9种灵猫科动物的88个骨骼性状、31个外部形态性状和1个行为性状,运用替代外群法分别以赤狐(犬科狐属)和青鼬(鼬科貂属)共同或单独作为外群进行支序分析,得出10个支序图(其步长(TL)为106--136,一致性指数(CI)为0.581--0.660,保留指数(RI)为0.610--0.714。经合意分析得到4个相似的支序图,其TL为41--136,CI为0.581--0.732,RI为0.610--0.818,结果支持:(1)斑灵狸、大灵猫、小灵猫构成一个单系群,与传统分类一致(均属灵猫亚科);(2)椰子狸、花面狸、熊狸构成一个单系群,与传统分类一致(均属长尾狸亚科);(3)红颊meng、食蟹meng与灵猫科其他种为姐妹关系(Bootstrap检验支持率100%),建议meng类为一亚科;(4)长颌带狸拥有较多自近裔性状如三个门齿孔,是一个高度特化的种类,其系统地位有待进一步研究。

Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis

Vascular endothelial growth factor (VEGF) plays an important role in normal and pathological angiogenesis. VEGF receptors (VEGFRs, including VEGFR-1, VEGFR-2, and VEGFR-3) and neuropilins (NRPs, including NRP-1 and NRF-2) are high-affinity receptors for V

广西岜盆白头叶猴的种群动态

岜盆保护区现有白头叶猴136只,分属19个两性群、5个独雄群和1个双雄群,平均群大小5.44只/群,群密度0.26群/km~(2),种群密度1.44只/km~(2),白头叶猴的年龄组成为:成年猴80只,占58.82%,青年猴36只,占26.47%,幼猴20只,占14.71%。

真核生物TATA框的折叠结构特征

通过对真核生物142个基因的部分启动子序列的折叠模型进行分析研究,结果表明,这些基因的TATA框在折叠结构模型中所表现的茎环结构特征有利于与反式作用因子和RNA聚合酶的识别及其相互作用。