Purification and characterization of two types of chitosanase from a Microbacterium sp.

Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.

Long-Wave Dynamics of Sea Level Variations during Indian Ocean Dipole Events

Long-wave dynamics of the interannual variations of the equatorial Indian Ocean circulation are studied using an ocean general circulation model forced by the assimilated surface winds and heat flux of the European Centre for Medium-Range Weather Forecasts. The simulation has reproduced the sea level anomalies of the Ocean Topography Experiment (TOPEX)/Poseidon altimeter observations well. The equatorial Kelvin and Rossby waves decomposed from the model simulation show that western boundary reflections provide important negative feedbacks to the evolution of the upwelling currents off the Java coast during Indian Ocean dipole (IOD) events. Two downwelling Kelvin wave pulses are generated at the western boundary during IOD events: the first is reflected from the equatorial Rossby waves and the second from the off-equatorial Rossby waves in the southern Indian Ocean. The upwelling in the eastern basin during the 1997-98 IOD event is weakened by the first Kelvin wave pulse and terminated by the second. In comparison, the upwelling during the 1994 IOD event is terminated by the first Kelvin wave pulse because the southeasterly winds off the Java coast are weak at the end of 1994. The atmospheric intraseasonal forcing, which plays an important role in inducing Java upwelling during the early stage of an IOD event, is found to play a minor role in terminating the upwelling off the Java coast because the intraseasonal winds are either weak or absent during the IOD mature phase. The equatorial wave analyses suggest that the upwelling off the Java coast during IOD events is terminated primarily by western boundary reflections.

A microwave emissivity model of sea surface under wave breaking

With the effective medium approximation theory of composites, a remedial model is proposed for estimating the microwave emissivity of sea surface under wave breaking driven by strong wind on the basis of an empirical model given by Pandey and Kakar. In our model, the effects of the shapes of seawater droplets and the thickness of whitecap layer (i.e. a composite layer of air and sea water droplets) over the sea surface on the microwave emissivity are investigated by calculating the effective dielectric constant of whitecaps layer. The wind speed is included in our model, and the responses of water droplets shapes, such as sphere and ellipsoid, to the emissivity are also discussed at different microwave frequencies. The model is in good agreement with the experimental data of microwave emissivity of sea surface at microwave frequencies of 6.6, 10.7 and 37GHz.

Effects of rearing temperature and density on growth, survival and development of sea cucumber larvae, Apostichopus japonicus (Selenka)

In laboratory conditions, effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively. Data series like larval length and density, metamorphic time, and survival rate of the larvae were recorded. Statistics showed that for A. japonicus, survival rate (from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature (P < 0.05). At different temperatures SGR was statistically significant as well (P < 0.05) from day 1, and maximal SGR was found on day 9 at 24A degrees C (159.26 +/- 3.28). This study clearly indicated that at low temperature (< 24A degrees C), metamorphic rate was remarkably higher than at higher temperature (> 26A degrees C). Hatching rate was significantly different between 0.2-5 ind./ml groups and 20-50 ind./ml groups. Rearing larvae at the higher density had the smaller maximal-length, whereas needed longer time to complete metamorphosis. This study suggested that 21A degrees C and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large -scale artificial breeding of A. japonicus's larvae.

Metabolic characteristics of sea cucumber Apostichopus japonicus (Selenka) during aestivation

Metabolic characteristics of the sea cucumber Aposticholpus japonicus (Selenka) during aestivation were studied in the laboratory. The effects of water temperature on oxygen consumption rate (OCR) and ammonia-N excretion rate (AER) in A. japonicus were determined by the Winkler and Hypobromite methods, respectively. Mature (large, 148.5 +/- 15.4 g, medium 69.3 +/- 6.9 g) and immature (small, 21.2 +/- 4.7 g) individuals aestivated at water temperatures of 20 and 25 degrees C, respectively. The metabolic characteristics of mature individuals were different from immature individuals during this period. The OCR of mature sea cucumbers peaked at 20 degrees C, and then dropped significantly at higher temperatures, whereas the OCR of the immature animals continued to increase slightly, even beyond the aestivation temperature. The AER of mature individuals peaked at 20 T, while that of the immature animals peaked at 25 degrees C. The relationships between dry weight (DW) and absolute oxygen consumption (R) and absolute ammonia-N excretion (N) could be described by the regression equation R or N=aW(b). With the exception of 15 degrees C, the O/N ratios (calculated in atomic equivalents) of large size sea cucumbers was close to 20 across the temperatures used in this study, indicating that their energy Source was a combination of lipid and protein. Oil the other hand, apart from small individuals maintained at 10 degrees C, the O/N ratios of the medium and small sea Cucumbers were close to 10, indicating that protein was their major energy source. The O/N ratios in all size groups remained unchanged after aestivation was initiated. (c) 2005 Elsevier B.V. All rights reserved.

Phyllophorus (Phyllophorus) maculatus, a new species of sea cucumber from the Yellow Sea (Echinodermata : Holothuroidea : Dendrochirotida)

Phyllophorus (Phyllophorus) maculatus new species is described from a depth of 22-45 meters near the western edge of the Yellow Sea. The body is cylindrical, with body wall ossicles present only in the anal region. Ossicles are four-pillared tables with low spires and indented disc margin. A revised key to the eleven phyllophorine (Family Phyllophoridae, Subfamily Phyllophorinae) species now known from China is presented.

Spatial and temporal relationships between precipitation and ANPP of four types of grasslands in northern China

Precipitation is considered to be the primary resource limiting terrestrial biological activity in water-limited regions. Its overriding effect on the production of grassland is complex. In this paper, field data of 48 sites (including temperate meadow steppe,temperate steppe, temperate desert steppe and alpine meadow) were gathered from 31 published papers and monographs to analyze the relationship between above-ground net primary productivity (ANPP) and precipitation by the method of regression analysis. The results indicated that there was a great difference between spatial pattern and temporal pattern by which precipitation influenced grassland ANPP. Mean annual precipitation (MAP) was the main factor determining spatial distribution of grassland ANPP (r~2 = 0.61,P ＜ 0.01); while temporally, no significant relationship was found between the variance of AN PP and inter-annual precipitation for the four types of grassland. However, after dividing annual precipitation into monthly value and taking time lag effect into account, the study found significant relationships between ANPP and precipitation. For the temperate meadow steppe, the key variable determining inter-annual change of ANPP was last August-May precipitation (r~2= 0.47, P = 0.01); for the temperate steppe, the key variable was July precipitation (r~2 = 0.36, P = 0.02); for the temperate desert steppe, the key variable was April-June precipitation (r~2 = 0.51, P ＜0.01); for the alpine meadow, the key variable was last September-May precipitation (r~2 = 0.29, P ＜ 0.05). In comparison with analogous research, the study demonstrated that the key factor determining inter-annual changes of grassland ANPP was the cumulative precipitation in certain periods of that year or the previous year.

Comparative study on CO2 emissions from different types of alpine meadows during grass exuberance period

Potentilla fruticosa scrub, Kobresia humilis meadow and Kobresia tibetica meadow are widely distributed on the Qinghai-Tibet Plateau. During the grass exuberance period from 3 July to 4September, based on close chamber-GC method, a study on CO2 emissions from different treatments was conducted in these meadows at Haibei research station, CAS. Results indicated that mean CO2emission rates from various treatments were 672.09+152.37 mgm-2h-1 for FC (grass treatment); 425.41+191.99 mgrn-2h-1 for FJ (grass exclusion treatment); 280.36+174.83 mgrn-2h-1 for FL (grass and roots exclusion treatment); 838.95+237.02 mgm-2h-1 for GG (scrub+grass treatment); 528.48+205.67 mgm-2h-1for GC (grass treatment); 268.97 ±99.72 mgm-2h-1 for GL (grass and roots exclusion treatment); and 659.20±94.83 mgm-2h-1 for LC (grass treatment), respectively (FC, FJ, FL, GG, GC, GL, LC were the Chinese abbreviation for various treatments). Furthermore, Kobresia humilis meadow, Potentilla fruticosa scrub meadow and Kobresia tibetica meadow differed greatly in average CO2 emission rate of soil-plant system, in the order of GG＞FC＞LC＞GC. Moreover, in Kobresia humilis meadow,heterotrophic and autotrophic respiration accounted for 42% and 58% of the total respiration of soil-plant system respectively, whereas, in Potentilla fruticosa scrub meadow, heterotrophic and autotrophic respiration accounted for 32% and 68% of total system respiration from G-G; 49% and 51%from GC. In addition, root respiration from Kobresia humilis meadow approximated 145 mgCO2m-2h-1,contributed 34% to soil respiration. During the experiment period, Kobresia humilis meadow and Potentilla fruticosa scrub meadow had a net carbon fixation of 111.11 grn-2 and 243.89 grn-2,respectively. Results also showed that soil temperature was the main factor which influenced CO2 emission from alpine meadow ecosystem, significant correlations were found between soil temperature at 5 cm depth and CO2 emission from GG, GC, FC and FJ treatments. In addition, soil moisture may be the inhibitory factor of CO2 emission from Kobresia tibetica meadow, and more detailed analyses should be done in further research.