增温对川西北高寒草甸植物群落结构及几种主要植物生长和生理的影响

由于人类活动所引起的地球大气层中温室气体的富集已导致全球地表平均温度在20世纪升高了0.6 ℃，并预测在本世纪将上升1.4-5.8 ℃。气候变暖对陆地植物和生态系统影响深远，并已成为全球变化研究的重要议题。高海拔、高纬度地带的生态系统对气候变化最敏感。而在高原和高山极端环境影响下所形成的高寒草甸生态系统极其脆弱，对由于温室效应引起的全球气候变化极其敏感，对这些变化的响应更具有超前性。 本研究以川西北高寒草甸植物群落及几种主要物种为研究对象，采用国际山地综合研究中心（ITEX）普遍所采用的增温方法-----开顶式生长室（OTC）模拟气候变暖来研究增温对高寒草甸植物群落结构、物质分配及其主要物种生长和生理的影响，以探讨高寒草甸植物响应与适应气候变暖的生物学和生态学机制。主要研究结论如下： 1、OTC的增温效果 由于地温、地表温度和气温的平均值在OTC内分别高出对照样地0.28℃、0.46℃和1.4℃，这说明本研究所采用的开顶式生长室（OTC）起到了增温的作用；同时，由于温室内与温室外接受的降水量相同，温室内由于热量条件的改善，土壤蒸发和植被的蒸腾作用增强，直接导致了OTC内土壤表层相对湿度的减少。 2、群落结构对增温的响应 由于增温时间较短，增温内外样地的物种组成并未发生改变；但增温后一定程度上改变了植物群落的小气候环境，从而导致物种间的竞争关系被破坏，种间竞争关系的破坏引起群落优势种组成发生相应的改变，在对照样地，鹅绒委陵菜、甘青老鹳草、遏蓝菜和蚤缀是占绝对优势的物种，而在OTC内，小米草、尼泊尔酸模、垂穗披碱草、发草和羊茅的重要性显著增加。 禾草和杂草由于对增温的生物学特性及其资源利用响应的不同，加之增温造成土壤含水量下降等环境因子的改变。与对照样地相比较，OTC内禾草的盖度及生物量都显著增加，而杂草的盖度和生物量则显著下降。 3、植物生长期对增温的响应 OTC内立枯和调落物的生物量在生长季末（10月份）都要小于对照样地的立枯和调落物生物量，而OTC内的地上鲜体生物量在10月份却略高于对照样地。这说明OTC内植物的衰老或死亡得以延缓，而植物的生长期得以延长。 4、群落生物量及分配对增温的响应 OTC内的地上鲜体生物量(10月份除外)和地下0-30cm的根系生物量与对照样地相比较，都出现了不同程度的减少；土壤根系的分配格局也发生了明显的改变，其中，OTC内0-10cm土层的生物量分配比例增加，而20-30cm土层生物量分配比例的减少。 5、群落碳、氮对增温的响应 增温后，OTC内植物群落地上活体和地下活根的碳浓度不同程度的高于对照样地，植物群落的碳库在OTC内也略高于对照样地；而OTC内植物群落地上活体和地下活根的氮浓度不同程度的低于对照样地，其植物群落的氮库与对照样地相比也略有下降。 6、几种主要植物的生长及物质分配对增温的响应 垂穗披碱草在增温后株高、比叶面积和地上生物量均显著地增加；尼泊尔酸模在增温后比叶面积和单株平均生物量积累显著地增加，而各组分中，增温处理使叶的生物量显著增加，而根的生物量却显著下降；鹅绒委陵菜在增温后株高、比叶面积和单株平均生物量积累显著地减少，而各组分中，增温处理使叶和茎的生物量显著减少，根的生物量却显著地增加。 尼泊尔酸模的LMR、RMR、R/S、根部碳含量、碳和氮在叶片与根部的分配比例在增温后显著地增加，而SMR、根部氮含量、碳和氮在茎部的分配比例在增温后却显著地降低；鹅绒委陵菜的RMR、R/S、碳和氮在根部的分配比例在增温后显著地增加，而SMR、LMR、碳在叶片的分配比例在增温后却显著地降低 7、几种主要植物的光合生理过程对增温的响应 增温使垂穗披碱草和尼泊尔酸模叶片中的叶绿素a、叶绿素b、总叶绿素含量显著增加；而鹅绒委陵菜叶片的叶绿素a、叶绿素b、总叶绿素含量在增温后显著减少，类胡萝卜素含量在增温后却显著增加。 增温对3种植物的气体交换产生了显著影响。其中，垂穗披碱草和尼泊尔酸模叶片的光响应曲线在增温后明显高于对照处理，A、E、gs、Pmax、、Rday、AQY和LSP显著增加，而LCP则显著降低；鹅绒委陵菜的光响应曲线在增温后则明显的低于对照处理，A、E、gs、Pmax、、Rday、AQY和LSP显著减少，而LCP则显著增加。 增温后垂穗披碱草和尼泊尔酸模叶片的Fv/Fm、Yield和qP显著增加；而鹅绒委陵菜叶片的Fv/Fm、Yield和qP则显著减少，qN却显著地增加。 8、几种主要植物的抗氧化酶系统对增温的响应 增温使垂穗披碱草和尼泊尔酸模体内抗氧化酶活性和非酶促作用有所提高，植物膜脂过氧化作用降低；鹅绒委陵菜叶片中酶促反应和非酶促反应在增温后也显著提高，但可能由于增温后的土壤干旱超过了鹅绒委陵菜叶的抗氧化保护能力，抗氧化酶活性及非酶促反应（脯氨酸、类胡萝卜素）的提高不足以完全清除干旱诱导形成的过量活性氧，因此叶片的膜脂过氧化程度仍然显著提高。 Enrichment of atmospheric greenhouse gases resulted from human activities such as fossil fuel burning and deforestation has increased global mean temperature by 0.6 ℃ in the 20th century and is predicted to increase in this century by 1.4-5.8 ℃. The global warming will have profound, long-term impacts on terrestrial plants and ecosystems. The ecoologcial consequences arising from global warming have also become the very important issuses of global change research. The terrestrial habitats of high-elevation and high-latitude ecosystems are regarded as the most sensitive to changing climate. The alpine meadow ecosystme, which resulted from the composite effects of mountain extreme climatic factors in Tibetan Plateau, is thus thought to be especially vulnerable and sensitive to global warming. In this paper, the response of plant community and several main species in the alpine meadow of Northewst Sichuan to experimemtal warming was studied by using open-top chambers (OTC). The aim of the this study was to research the warming effects on plant community structure, substance allocation, growth and physiological processes of several mian species, and to explore the biological and ecological mechanism of how the alpine meadow plants acclimate and adapt to future global warming. The results were as follows: 1. Warming effects of OTC The mean soil temperature, soil surface temperature and air temperature in OTC manipulation increased by 0.28℃、0.46℃ and 1.4℃ compared to the control during the growing season. This suggested that the OTC used in our study had increased temperature there. Meanwhile, the OTC manipulation slightly altered thermal conditions, but the same amount of precipitation was supplied to both the OTC manipulation and the control, so higher soil evaporation and plant transpiration in OTC manipulation directly lead to the decrease of soil surface water content. 2. The reponse of community structure to experimental warming The species richness was not changed by the short-term effect of OTC manipulation. However, experimental warming changed the microenvironment of plant community, therefore competitive balances among species were shift, leading to changes in species dominance. In the present study, the dominant plant species in the control plots were some forbs including Potentilla anserine, Geranium pylzowianum, Thlaspi arvense and Arenaria serpyllifolia, however, the importance value of some gramineous grasses including Elymus nutans, Deschampsia caespitosa, Festuca ovina, and some forbs including Euphrasia tatarica and Rumex acetosa significantly increased in OTC. The different biology characteristics and resource utilizations between gramineous grasses and forbs, and enhanced temperature caused change in some environment factors such as soil water content. As a result, the coverage and biomass of gramineous grasses significantly increased in OTC compared to the control, however, the coverage and biomass of forbs singnifciantly decreased in OTC compared to the control. 3. The reponse of plant growing season to experimental warming Both the standing dead and fallen litter biomass in OTC were lower than those in the control in October, and the biomass of aboveground live-vegetation in OTC was higher than that of the control. The results indicated that the senescence of plants was postponed, and the growing season was prolonged in our research. 4. The reponse of community biomass accumulation and its allocation to experimental warming Experimental warming caused the decrease of aboveground live biomass and belowground root biomass except for the aboveground live biomass in October. Experimental warming also had pronounced effects on the pattern of root biomass allocation. In the present study, the root biomass in 0-10cm soil layer increased in OTC manipulation compared to the control, however, the root biomass in the 20-30cm soil layer decreased in OTC manipulation compared to the control. 5. The reponse of community C and N content to experimental warming The C concentration and stock in aboveground live and belowground root both increased in OTC manipulation compared to the control. However, the N concentration and stock in aboveground live and belowground root both decreased in OTC manipulation compared to the control. 6. The reponse of gowth and biomass, C and N alloction of several species to experimental warming Experimental warming significantly increased the height, SLA (specific leaf area) and aboveground biomass of Elymus nutans in OTC manipulation compared to the control. The SLA and total biomass of Rumex acetosa also significantly increased in OTC manipulation compared to control, among the different components of Rumex acetosa, leaf biomass significantly increased, but root biomass significantly decreased in OTC manipulation compared to the control. However, the height, SLA and total biomass of Potentilla anserina significantly decreased in OTC manipulation compared to the control, among the different component of Potentilla anserina, leaf and stem biomass significantly decreased, but root biomass significantly increased in OTC manipulation compared to the control. The LMR (leaf mass ratio), RMR (root mass ratio), R/S (shoot/root biomass ration) and root C concentration of Rumex acetosa significantly increased in OTC manipulation compared to outside control, also, Rumex acetosa allocated relatively more C and N content to leaf and root in response to experimental warming, however, the SMR (stem mass ration) and root N concentration of Rumex acetosa significantly decreased in OTC manipulation compared to outside control, also, Rumex acetosa allocated relatively less C and N content to stem in response to experimental warming. The RMR and R/S of Potentilla anserina significantly increased in OTC manipulation compared to outside control, also, Potentilla anserina allocated relatively more C and N content to root in response to experimental warming, however, the SMR and LMR of Potentilla anserina significantly decreased in OTC manipulation compared to outside control, also, Potentilla anserina allocated relatively less C and N content to leaf in response to experimental warming. 7. The reponse of physiological processes of several species to experimental warming Experimental warming significantly increased chlorophyll a, chlorophyll b and total chlorophyll of Elymus nutans and Rumex acetosa in OTC manipulation compared to outside control. However, chlorophyll a, chlorophyll b, total chlorophyll and carotenoid of Potentilla anserina in OTC manipulation significantly decreased compared to outside control. Experimental warming had pronounced effects on gas exchange of Elymus nutans, Rumex acetosa and Potentilla anserine. In the present study, warming markedly increased the light response curves of Elymus nutans and Rumex acetosa in OTC manipulation compared to outside control, and also singnificantly increased A (net photosynthesis rate), E (transpiration rate), gs (stomatal conductance), Pmax (maximum net photosynthetic rate), Rday (dark respiration rate), AQY (apparent quantum yield) and LSP (light saturation point), but LCP (photosynthetic light compensation) of Elymus nutans and Rumex acetosa in OTC manipulation singnificantly decreased compared to outside control. However, warming markedly decreased the light response curves of Potentilla anserina in OTC manipulation compared to outside control, and also singnificantly decreased A, E, gs, Pmax, Rday, AQY and LSP, but LCP of Potentilla anserina in OTC manipulation singnificantly increased compared to outside control. Experimental warming singnificantly increased the chlorophyll fluorescence kinetics parameters such as Fv/Fm, Yield and qP of Elymus nutans and Rumex acetosa and qN of Potentilla anserina in OTC manipulation, but Fv/Fm, Yield and qP of Potentilla anserina in OTC manipulation singnificantly decreased. 8. The reponse of antioxidative systems of several species to experimental warming Experimental warming tended to increase the activities of antioxidative enzymes and stimulate the role of non-enzymes of Elymus nutans and Rumex acetosa. As a result, MDA content of Elymus nutans and Rumex acetosa decreased. The activities of antioxidative enzymes and non-enzymes of Potentilla anserina also significantly increased in OTC manipulation, but more O2- was produced because of lower soil water content, and the O2- accumulation exceeded the defense ability of antioxidative systems and non-enzymes fuctions. As a result, MDA content of Potentilla anserine still increased in OTC manipulation compared to outside control.

生姜化感作用机理及其栽培模式研究

近年来，随着对作物重茬（连年种植）障碍原因的深入研究，植物的化感作用越来越受到国内外众多学者的重视。而作为重要调料和药用植物的生姜，其连作障碍也备受关注，系统地研究生姜化感作用将有助于理解和最终解决生姜连作障碍问题。本文通过研究生姜不同部位、不同浓度的水浸液对与其间作的两个物种（大豆和四季葱）种子的萌发及幼苗生长的影响，从而证明生姜化感作用的存在；并通过温室盆栽实验研究了生姜的自毒作用（即研究生姜不同部位、不同浓度的水浸液对其幼苗的形态、生理生化、光合作用、土壤酶、土壤微生物多样性及土壤养分的影响），从而揭示生姜退化和衰老的机制，并为生姜筛选出合适的间作物种提供科学依据，对生姜连作障碍提出科学的解决方法。主要研究结果如下： 1. 与对照相比，生姜所有部位（根茎、茎、叶）、所有浓度（10、20、40、 80 g l-1）的水浸液均抑制了大豆种子和葱籽的萌发率、幼苗生长、水分吸收和脂肪酶活性，并且其抑制程度随着水浸液浓度的增加而增强，其生姜各部位水浸液抑制效应的强弱顺序为茎＞叶＞根茎。这一结果表明生姜根茎、茎、叶含有能够抑制大豆种子和葱籽种子萌发和幼苗生长的水溶性化感物质。根茎是生姜的主要收获部位，而生姜的残株（主要是茎和叶）应该从大田中处理掉以减轻其抑制效应。生姜水浸液中主要化感成分包括：根茎水浸液中主要是丁香酸和伞花内脂；茎水浸液中主要是阿魏酸，且其含量最高为73.4 ug/g；叶水浸液中除了阿魏酸，其他六种物质均检测出来，但含量较高的主要有丁香酸、伞花内脂和香豆酸。 2. 生姜茎和叶不同浓度的水浸液均显著抑制了生姜幼苗的株高、每株叶片数和叶面积，其抑制程度随着水浸液浓度的增加而有所增强，而生姜幼苗每株分枝数差异不显著；同时生姜水浸液也极大程度地影响了生姜幼苗的生物量（包括地下生物量、地上生物量和总生物量，均为鲜重）。在同一浓度下，茎水浸液对生姜幼苗形态指标及生物量指标均显示出最强的抑制作用，叶水浸液次之，根茎水浸液最弱。与对照相比，低浓度的生姜根茎水浸液提高了生姜幼苗叶片内四种抗氧化酶（SOD、POD、CAT、APX）活性，高浓度的根茎水浸液抑制了四种抗氧化酶活性，而茎和叶水浸液均随着浓度的增加而抑制了四种抗氧化酶活性，三种水浸液均随着浓度的增加降低了生姜幼苗叶片内叶绿素的含量，而增加了生姜幼苗叶片的相对电导率和丙二醛含量。同时，三种水浸液均随着浓度的增加降低了生姜幼苗的光合参数（包括胞间CO2浓度、气孔导度、蒸腾速率及净光合速率）。 3. 三种生姜水浸液对所测六种土壤酶活性均产生了不同程度的影响，其中影响最大的是酸性磷酸酶和蔗糖酶，在10 g l-1 时就达到了显著水平，并且所有酶均有随着水浸液浓度增加而增大的趋势；相同部位的水浸液随着浓度的增加，细菌和真菌的数量呈增加趋势，而放线菌的数量呈减少趋势；三种生姜水浸液均随着浓度的增加降低了土壤中有机质的含量，加剧了土壤中硝态氮含量的积累，根茎水浸液对土壤有效磷、速效钾和铵态氮均显示出低浓度提高其含量而高浓度降低其含量的趋势，而茎和叶水浸液则随着浓度的增加均降低了其含量。 4. 与生姜单作相比，所有间作系统均在旺盛生长期和收获期不同程度地提高了土壤酶活性，同时也增加了土壤细菌数量及土壤微生物总数但不显著；所有间作系统在旺盛生长期和收获期均不同程度地影响了土壤真菌及放线菌数量（增加或减少），所有间作系统间的多样性指数差异不显著，除了旺盛生长期四种作物（生姜-大豆-四季葱-大蒜）的间作模式显著降低了多样性指数，其值仅为生姜单作的33.18%；生姜与大豆间作不仅提高了19.6%的生姜产量而且获得了较好的经济效益，并且，所有间作系统均显著抑制了生姜姜瘟病的发生。 5. 不同栽培模式不同程度地影响了收获期生姜的株高、分枝数、根茎产量及内在品质。其中处理2显著地促进了生姜的分枝（10.5%），同时处理2、3和4也促进了生姜的生长（株高分别增加了15.0%、11.4%和14.0%），并且这三个处理提高了生姜的产量；处理2和3能有效提高生姜块茎中维生素C（分别较单作生姜显著提高了3.29%和4.05%）、处理3显著提高了可溶性糖（8.2%）、姜辣素（4.6%）和蛋白质等有益物质的含量，降低硝酸盐有害物质的含量（处理2显著降低了14.0%），改善了姜块的外观和内在品质。并且，生姜与大豆间作具有最高的纯收入和产投比，分别较生姜单作提高了24.80%和8.8%。Recently, allelopathy has been more and more paid attentions by national and foreign scholars with profound research on reasons of crop replanted (continuous planted) obstacle. Ginger rhizome is valuable all over the world either as a spice or herbal medicine and ginger replanted obstacle is also paid attentions. Systematic research on ginger allelopathy will contribute to understanding and ultimate solving problem of ginger replanted obstacle. The effects of ginger aqueous extracts with different parts and concentrations on seed germination and early seedling growth of soybean and chive were studied in this article to testify that ginger existed allelopathy. Furthermore, ginger autotoxicity was also studied by pot experiment in greenhouse (namely research on effects of ginger aqueous extracts with different parts and concentrations on morphological indexes, physiological and biochemical indexes, photosynthesis, soil enzymes, soil microbial diversity and soil nutrients) to reveal mechanism of ginger degeneration and senescence, provide scientific basis for selecting appropriate intercropping species and put forward scientific resolvent for ginger replanted obstacle. The main results were as follows: 1. All aqueous extracts at all concentrations inhibited seed germination, seedling growth, water uptake and lipase activity of soybean and chive compared with the control, and the degree of inhibition increased with the incremental extracts concentration. The degree of toxicity of different ginger plant parts can be classified in order of decreasing inhibition as stem＞leaf＞rhizome. The results of this study suggested that rhizome, stem and leaf of ginger contained water soluble allelochemicals which could inhibit seed germination and seedling growth of soybean and chive. The rhizome is the main harvested part of ginger. The residue (mainly stems and leaves) of the ginger plant should be removed from the field so as to diminish its inhibitory effect. The main allelopathic components of three kind of aqueous extracts were as follows: Rhizome extract chiefly contained syringic acid and vmbelliferone and stem extract mainly contained frulic acid whose content was the highest (73.4 ug/g). The other six substances were detected except of frulic acid, but only contents of syringic acid, vmbelliferone and p-coumaric acid were higher. 2. Stem and leaf aqueous extracts of ginger with different concentrations significantly inhibited plant height, leaf numbers per plant and leaf area, and the degree of inhibition increased with the incremental extracts concentration. However, tiller number per plant of ginger seedling showed no significant difference. At the same time, ginger aqueous extracts also influenced biomass including under-ground biomass, above-ground biomass and total biomass (fresh weight) to a large extent. Under the same concentration, stem aqueous extract showed the mostly inhibitory effect on morphological indexes and biomass indexes of ginger seedling. Rhizome aqueous extract showed the leastly inhibitory effect and leaf aqueous extract was intervenient. Enhanced concentration of ginger aqueous extracts significantly reduced total chlorophyll content, accompanying with increases in memberane permeability (REL) and lipid peroxidation (MDA). Compared with the control, rhizome ginger aqueous extract of lower concentration (10 g l-1) increased the activities of major antioxidant enzymes (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; ascorbate peroxidase, APX) of ginger leaf tissue and higher concentration inhibited the activities of four antioxidant enzymes. However, stem and leaf aqueous extract inhibited the activities of four antioxidant enzymes with increase in concentration. Meanwhile, enhanced concentration of ginger aqueous extracts significantly reduced photo-parameters of ginger seedling (including CO2 concentration, stoma conductivity, net photosynthesis rate and transpiration rate). 3. Rhizome, stem and leaf ginger aqueous extract showed different effect on six soil enzyme activities, and acid phosphatase and invertase showed significant effect when aqueous extract concentration got 10 g l-1. Furthermore, six soil enzyme activities increased with increase in aqueous extract concentration. Bcterial and fungi number tended to increase while antinomyces tented to decrease with the increase in aqueous extract concentration of identical part. Ginger aqueous extracts reduced soil organic matter content with increased concentration, accompanying with NO3-—N accumulation in soil. Rhizome aqueous extract showed the same tendency for available P, available K and NH4+—N, namely lower concentration increased their contents in soil and higher concentration reduced their contents. While stem and leaf aqueous extracts reduced their contents with the increamental concentration. 4. All intercropping systems increased soil enzyme activities to different extent both at VGS and at HS compared to solo ginger. All intercropping systems increased the colony numbers of soil bacteria and total of soil microbe but not significantly either at VGS or at HS. All intercropping systems increased the colony numbers of soil fungi and actinomytes to a different extent (increase or decrease) both at VGS and at HS. For DI, difference between all cultivation patterns and S-G was not significant either at VGS or at HS except that G-S-C-G whose value was only 33.18% of S-G at VGS significantly decreased. G-S not only increased ginger yield by 19.6% but also obtained better economic benefit. Furthermore, all intercropping systems significantly inhibited occurrence of bacterial wilt of ginger. 5. Different cultivated pattern influenced plant height, tiller numbers, rhizome yields and intrinsic quality of ginger. Treatment 2 significantly facilitated tiller occurring (10.5%). Treatment 2, 3 and 4 promoted ginger growth (plant height respectively increased 15.0%、11.4% and 14.0%) and enhanced rhizome yields. Treatment 2 and 3 effectively increased vitamin C content (significantly increased 3.29% and 4.05% compared to solo ginger). Treatment 3 significantly increased contents of beneficial substances such as soluble sugar (8.2%), gingerols (4.6%) and protein. Treatment 2 significantly decreased contents of deleterious substance namely nitrate (14.0%) and improved appearance and intrinsic quality of ginger rhizome. Furthermore, treatment 2 (ginger/soybean intercropping) could obtain better economic benefit and showed the highest net income and ratio of benefit and cost whose values respectively increased by 24.80% and 8.8% compared to solo ginger.

Quantitative sample injection for capillary electrophoresis

An apparatus including a rotary-type injector was designed for quantitative sample injection in capillary electrophoresis (CE), in which both pressurized flow and electroosmotic flow were used to drive the background electrolyte solution. A relative standard deviation of peak area of lower than 1% was achieved by using this apparatus. The effects of back-pressure regulator, restrictor, and applied voltage on separation efficiency and resolution were investigated. The utility of this apparatus in both micro-HPLC and pressurized capillary electrochromatography (pCEC) was also demonstrated.

A model study on carbon cycle and phytoplankton dynamical processes in the Bohai Sea

The carbon cycle of lower trophic level in the Bohai Sea is studied with a three-dimension-al biological and physical coupled model. The influences of the processes (including horizontal advection,river nutrient load, active transport etc. ) on the phytoplankton biomass and its evolution are estimated.The Bohai Sea is a weak sink of the CO2 in the atmosphere. During the cycle, 13.7% of the gross pro-duction of the phytoplankton enter the higher trophic level and 76.8 % of it are consumed by the respira-tion itself. The nutrient reproduction comes mainly from the internal biogeochemical loop and the rem-ineralization is an important mechanism of the nutrient transfer from organic form to inorganic. Horizon-tal advection decreases the total biomass and the eutrophication in some sea areas. Change in the nutrientload of a river can only adjust the local system near its estuary. Controlling the input of the nutrient,which limits the alga growth, can be very useful in lessening the phytoplankton biomass.

黄土塬区旱作农田高生产力的水分环境效应与产量波动性

以长武生态站水肥产量效应的长期定位试验资料为基础，结合模型预测的分析方法，揭示出黄土高原旱作塬区高产农田的水分环境效应在于过度消耗土壤水库，降低土壤含水量，使作物生长转而过度依靠年度降水，出现年际产量的高波动性。指出旱作高产高波动性具有可持续性，它是当前旱作农业的基本特点。

侵蚀条件下坡地土壤水分与有效磷的空间分布特征

坡地的水土流失及磷素在泥沙中的富集不仅导致土地生产力下降、环境恶化 ,也对下游水体的环境造成严重危害。以不同施磷时间的黄绵土为试验材料 ,通过人工模拟降雨试验观察了坡地侵蚀土壤水分含量 (SM)与有效磷含量 (APC)的空间变化特征。结果表明 :自坡顶向下侵蚀土壤表层 0～ 5 cm与 5～ 10 cm的土壤水分 (SM)呈现出完全一致的波浪状递增趋势。有效磷含量 (APC)的空间变化虽然也为波浪状 ,但其节奏要较水分变化快一倍 ,而且 0～ 5 cm与 5～ 10 cm两层间并不同步 ,下层略有滞后。磷向 2 0 cm以下土层的迁移量极少 ,不同坡位间差别不大。施磷 2 4d以前从坡顶向下有效磷含量 (APC)呈降低趋势 ,而施磷 2 4d以后在坡下则略有富集 ;施磷 6 d以前坡地 0～ 5 cm土层有效磷含量 (APC)较 6 d以后低 8%左右

Biologically Inspired Features for Scene Classification in Video Surveillance

Inspired by human visual cognition mechanism, this paper first presents a scene classification method based on an improved standard model feature. Compared with state-of-the-art efforts in scene classification, the newly proposed method is more robust, more selective, and of lower complexity. These advantages are demonstrated by two sets of experiments on both our own database and standard public ones. Furthermore, occlusion and disorder problems in scene classification in video surveillance are also first studied in this paper.

Triterpane and sterane biomarkers in the YA13-1 condensates from Qiongdongnan Basin, South China Sea

Triterpanes and steranes in condensates from the YA13-1 gas field, Qiongdongnan Basin, were monitored. The YA13-1 condensates have unusual biomarker distributions dominated by terpanes and steranes derived from higher plants. Anomalously abundant 1 got-oleanane and remarkably abundant bicadinanes are present in the YA13-1 condensates, whereas the 17alpha-hopane contents are extremely low. Taraxastane and significantly abundant 17alpha-diahopanes occur in the condensates. In addition, a number of unknown C-29 and C-30 pentacyclic triterpanes including previously unreported compounds were detected in the condensates, some of which are significantly abundant. The unknown compounds may be terrestrial biomarkers. C-29 homologues are relatively predominant among the regular and rearranged steranes. The diasterane concentrations are markedly higher than those of regular steranes. The maturity of the YA13-1 condensates is relatively high, at the peak to late oil generation stage (corresponding to 0.85-1.10% Ro), based on sterane and terpane and including bicadinane maturity parameters (i.e. T/(T-1 + R) and 2T/R bicadinane ratios). The above maturity assessment result is different from that based on diamondoid maturity parameters (%Ro = 1.60-1.70) [Org. Geochem. 25 (1996) 179], which can be explained by a contribution of hydrocarbons from two sources at different depths. The YA13-1 condensates were probably generated from the Yacheng and Lingshui coal-bearing source rocks buried both in the Qiongdongnan Basin (3400-5000 m) and in the Yinggehai Basin (>5000 m). The possible contribution of lower maturity hydrocarbons from the Yacheng and Lingshui Formations (3400-4100 m) in the Qiongdongnan Basin to the YA13-1 gases and condensates should not be neglected. (C) 2003 Elsevier Science B.V. All rights reserved.

冀东油田南堡滩海地区油气层损害机理与油气层保护方法研究及应用

Study and Application of Damage Mechanism and Protection Method of reservoir in Nanpu Shallow Beach Sea Area is one of the key research projects of Jidong Oilfield Company of PetroChina Company Limited from 2007 to 2008. Located at Nanpu Sag in Huanghua Depression of Bohaiwan Basin, Nanpu Shallow Beach Sea Area with 1000km2 exploration area posseses three sets, shallow Minghuazhen Formation and Guantao Formation of Upper Tertiary, middle-deep Dongying Formation of Lower Tertiary, deep Ordovician, of oil bearing series, according to the achievement of the connecting 3D seismic structure interpretation and the structural geological comprehensive research. Its main reservoir types include Upper Tertiary structural reservoir, Lower Tertiary structural and lithological-structural reservoir, and Ordovician ancient buried hill reservoir. How to protect reservoir, complete well and lift high efficiently is the key to realize high and stable yield of the oil wells during drilling, completing well, testing and repairing well. It is important for reservoir protecting during drilling that directly relate to efficient exploration. Therefore, beginning with basic characteristics and sensitive analysis of reservoir, study of reservoir damage machinism and analysis of reservoir damage potential factor are emphasized when prediction analysis about three-pressure profiles is carried out. The study both of physical and chemical properties and of the strata of the technology of borehole stabilization and reservoir protecting are outstanding. As the conclusions follow: （1）Based on the laboratory experiment about basalt cores, prediction of three- pressure profiles about 30 wells on No.1 and No.2 structure is practiced. The laws of plane pressure distribution are analyzed. （2）According to the analyses about reservoir feature data and about sensitivity evaluation to damage factor in Nanpu oil field, the scheme of reservoir protecting to the sand reservoir of Guantao Formation and the first section of Dongying Formation is put forward. （3）On basis of the analyses on lithological characteristics, mineral compositions, clay minerals, electrical behavior features, physical and chemical properties of basalt of Guantao formation in No.1 and No.2 structure, instability mechanism of basalt sidewall and technical countermeasures are obtained. （4）Aiming at the characteristics of Ordovician dissolution-pore fracture type carbonate reservoir, the scheme of the reservoir protecting to Ordovician is put forward. Creative study of the film forming and sealing and low invasion reservoir protection drilling fluid are successful. In summary, through the study of reservoir heterogeneity and sensitivity, a set of technology and schemes of reservoir protecting is put forward, which is adaptive during drilling the target bed in the research area and establishes the base for efficient exploration. Significant effect has showed in its application in Nanpu oil field.

砂砾岩油藏精细油藏描述–以准噶尔盆地西北缘八区下乌尔禾组油藏为例

Glutenite reservoir is one of the most important reservoir types in china. Because of its particularity of rock structure and pore structure, it is usually difficult in development, especially for its serious heterogeneity. On the basis of seismic, well logs, core data and production performance, the lower Wuerhe group can be divided into one second-order sequences, two third-order sequences and twenty two subsequences, corresponding to the five stages and twenty two minlayers. In addition, the fault systems are interpreted and the control action of fault systems to reservoir development is also described. The lower Wuerhe formation of 8th district belongs to fluvial-dominated fan delta sedimentation, according to the analysis of well logs, logging data and core data. It can be subdivided into two kinds of subfacies and nine kinds of microfacies. The fan delta plain subfacies mainly consist of braided channel, unconcentrated flow, mud flow and sieve deposit microfacies. The fan delta front subfacies include subaqueous distributary channel, subaqueous interdistributary channel, debris flow, subaqueous barrier and grain flow microfacies. Combined with the regional geological characteristics, the porosity model of lower Wuerhe formation is performed using core data. A permeability model based on the flow zone index is also formed according to the pore throat characteristics and flow property. Finally, the heterogeneity is analyzed. The result shows that the lower Wuerhe formation has a feature of middle-high heterogeneity, and it is controlled by material sources and sedimentary facies belt.

中高层大气对低层大气声重波响应的分析

Acoustic Gravity waves (AGW) play an important role in balancing the atmospheric energy and momentum budget. Propagation of gravity wave in the atmosphere is one of the important factors of changing middle and upper atmosphere and ionosphere. The purpose of this dissertation is to study the propagation of gravity wave in a compression atmosphere whit means of numerical simulation and to analyze the response of middle and upper atmosphere to pulse disturbance from lower atmosphere. This work begins with the establishment of 2-D fully nonlinear compressible atmospheric dynamic model in polar coordinate, which is used ton numerically study gravity wave propagation. Then the propagation characteristics of acoustic gravity wave packets are investigated and discussed. We also simulate the response of middle and upper atmosphere to pulse disturbance of lower atmosphere in background winds or without background winds by using this model and analyze the data we obtained by using Fourier Transform (FT), Short-time Fourier Transform (STFT) and Empirical Mode Decomposition (EMD) method which is an important part of Hilbert-Huang Transform (HHT). The research content is summarized in the following: 1. By using a two-dimensional full-implicit-continuous-Eulerian (FICE) scheme and taking the atmospheric basic motion equations as the governing equations, a numerical model for nonlinear propagation of acoustic gravity wave disturbance in two-dimensional polar coordinates is solved. 2. Then the propagation characteristics of acoustic gravity wave packets are investigated and discussed. Results of numerical simulation show that the acoustic gravity wave packets propagate steadily upward and keep its shape well after several periods. 3. We simulate the response of middle and upper atmosphere to pulse disturbance of lower atmosphere in background winds or without background winds by using this model, and obtain the distribution of a certain physical quantity in time and space from earth’s surface to 300km above. The results reveal that the response of ionosphere occurs at a large horizontal distance from the source and the disturbance becomes greater with increasing of height. The situation when the direction of the background wind is opposite to or the same as the direction of disturbed velocity of gravity-wave is studied. The results show that gravity wave propagating against winds is easier than those propagating along winds and the background wind can accelerate gravity wave propagation. Just upon the source, an acoustic wave component with period of 6 min can be found. These images of simulation are similar to observations of the total electron content (TEC) disturbances caused by the great Sumatra-Andaman earthquake on December 26 in 2004. 4. Using the EMD method the disturbed velocity data of a certain physical quantity in time and space can be decomposed into a series of intrinsic mode function (IMF) and a trend mode respectively. The results of EMD reveal impact of the gravity wave frequency under the background winds.

印度与欧亚板块强烈相互作用区深部结构特征研究

The central-south Tibet is a part of the products of the continental plate collision between Eurasia and India. To study the deep structure of the study area is significant for understanding the dynamics of the continental-continental collision. A 3-D density model matched well with the observations in the central-south Tibet was proposed in this study. In addition, this study has also used numerical simulation method to prove that Quasi-Love (QL) wave is deduced by anisotropy variation but not by lateral heterogeneity. Meanwhile, anisotropy variation in the upper mantle of the Qiangtang terrane and Lhasa terrane is detected by the QL waves observed in recorded seismograms. Based on the gravity modeling, some results are summarized as follows: 1) Under the constrain of geometrical structure detected by seismic data, a 3-D density model and Moho interface are proposed by gravity inversion of the central-south Tibet. 2) The fact that the lower crustal densities are smaller than 3.2 g/cm3, suggests absence of eclogite or partial eclogitization due to delamination under the central-south Tibet. 3) Seismicity will be strong or weak in the most negative Bouguer gravity anomaly. So there is no a certain relationship between seismicity and Bouguer gravity anomaly. 4) Crustal composition are determined after temperature-pressure calibration of seismic P wave velocity. The composition of lower crust might be one or a mixture of: 1. amphibolite and greenschist facies basalt beneath the Qiangtang terrane; 2. gabbro-norite-troctolite and mafic granulite beneath the Lhasa terrane. Because the composition of the middle crust cannot be well constrained by the above data set, the data set published by Rudnick & Fountain (1995) is used for comparison. It indicated the composition of the middle crust is granulite facies and might be pelitic gneisses.Granulite facies used to be interpreted as residues of partial melting, which coincidences with the previous study on partial melting middle crust. Amphibolite facies are thought to be produced after delamination, when underplating works in the rebound of the lower crust and lithospheric mantle. From the seismology study, I have made several followed conclusions: 1) Through the numerical simulation experiment of surface wave propagating in heterogeneity media, we can find that amplitude and polarization of surface wave only change a little when considering heterogeneity. Furthermore, it is proved that QL waves, generated by surface wave scattering, are caused by lateral variation of anisotropy but not by heterogeneity. 2) QL waves are utilized to determine the variation of uppermost mantle anisotropy of the Tibetan plateau. QL waves are identified from the seismograms of the selected paths recorded by the CAD station. The location of azimuth anisotropy gradient is estimated from the group velocities of Rayleigh wave, Love wave and QL wave. It suggests that south-north lateral variation of azimuthal anisotropy locates in Tanggula mountain, and east-west lateral variation in the north of Gandese mountain with 85°E longitude and near the Jinsha river fault with 85°E longitude.

塔里木盆地塔北、塔中地区早古生代白云岩形成机理

In Tarim Basin, extensive carbonates of Lower Paleozoic occur, in which thick Cambrian and Lower Ordovician dolostones are widespread and show a potential perspective in hydrocarbon exploration. So they are viewed as an important target for exploration. Tarim Basin is a poly phase composite basin, which underwent multiphase tectonic modification and volcanic activities; these exerted significant influences on the basin-fills and basin fluid evolution, thereby the diagenetic history, particularly on the deep-buried Lower Paleozoic dolostones. Referring to the classification of dolomite texture proposed by Gregg & Sibley (1984) and Sibley & Gregg (1987). In view of crystal size, crystal shape, crystal surface and contact relation, eight genetic textures of dolomite crystals are identified, based on careful petrographic examinatoins. These textures include: 1) micritic dolomite; 2) relict mimetic dolomite; 3)finely crystalline, planar-e(s), floating dolomite; 4)finely crystalline, planar-e(s) dolomite; 5) finely-coarse crystalline, nonplanar-a dolomite; 6)coarse crystalline, nonplanar saddle dolomite; 7) finely-medium crystalline, planar-e(s) dolomite cement; 8) coarse crystalline, nonplanar saddle dolomite cement, in which the former six textures occurs as in matrix, the latter two in the cements. Detailed geochemistry analysis is carried out on the basis of genetic textures of dolomite and related minerals such as quartz and calcite. The result showed that the calcite has the highest average content in Sr, which can be sorted into two groups; micritic dolomite has the highest average content in Sr among all kinds of dolomites; the REE patterns of all kinds of dolomites is similar to those of marine limestone samples. Saddle dolomite cement has δ13C values from -2.44‰ to 1.27‰ PDB, and δ18O values from -13.01‰ to -5.12‰ PDB, which partially overlap with those of matrix dolomite (δ13C values from -2.83‰ to 2.01‰ PDB, δ18O values from -10.63‰ to -0.85‰ PDB). Saddle dolomite cement has 87Sr/86Sr ratios from 0.7086 to 0.7104, which totally overlap with those of matrix dolomite (0.7084 ~ 0.7116). Compared with saddle dolomite derived from other basins all over the world, the saddle dolomites of Tarim Basin have similar δ13C, δ18O and 87Sr/86Sr ratios values with those of matrix dolomite. This scenario reflects the unusual geological setting and special dolomitizing liquid of Tarim Basin. The values of δ18O, δ13C and 87Sr/86Sr ratios of calcite also can be sorted out two groups, which may been resulted from the one stage of extensive uplift of Tarim Basin from Mesozoic to Cenozoic. Fluid inclusion microthermometry data of the diagenetic mineral indicates that matrix dolomite has relatively low homogenization temperatures (Th) of 80～105oC and salinities of 12.3％ (wt% NaCl equivalent); saddle dolomite has highest Th values, which concentrate in 120～160oC and salinities of 13.5～23.7％ (wt% NaCl equivalent); quartz has relatively low Th of 135～155oC and salinities of 17.8～22.5％ (wt% NaCl equivalent); calcite has relatively low Th of 121～159.5oC and salinities of 1.4～17.5％ (wt% NaCl equivalent). These data suggest that the saddle dolomites could have formed in thermal brine fluids. Based on comprehensive petrographical study, detailed geochemistry and fluid inclusion microthermometry analysis on Lower Paleozoic dolomite of Tarim Basin, three types of dolomitisation mechanism are proposed: Penecontemporaneous dolomitisation (Sabkha dolomitisation & Reflux dolomitisation); Burial dolomitisation (shallow-intermediate burial dolomitisation & Deep burial dolomitisation ); Hydrothermal cannibalized dolomitisation. In view of host-specified occurrences of hydrothermal dolomite, the low abundance of saddle dolomite and high geochemical similarities between saddle dolomite and host dolomite, as well as highest Th and high salinities , the hydrothermal dolomite in Tarim Basin is thus unique, which could have been precipitated in modified fluid in the host dolomite through intraformational thermal fluid cannibalization of Mg ions from the host. This scenario is different from the cases that large scale dolomitizing fluid migration took place along the fluid pathways where abundant saddle dolomite precipitated. Detailed observations on 180 petrographic and 60 casting thin sections show original pores in Lower Paleozoic dolomite were almost died out by complicated diagenetic process after a long time geologic evolution. On the other hand, deep-buried dolomite reservoirs is formed by tectonic and hydrothermal reforming on initial dolomites. Therefore, the distribution of structure-controlled hydrothermal dolomite reservoirs is predicted in Tabei and Tazhong Area of Tarim Basin based on the geophysical data.

蒙古国宗巴音凹陷沉积相研究及优质储层预测

Abstract:Little fundamental work on petroleum exploration and production of Zuunbayan Subbasin, Mongolia has been done before because of the backward economy and petroleum industry techniques in this country, which also results in our little knowledge of reservoir characteristics of this area. This paper focused on the sedimentary system, sedimentary facies, reservoir characteristics and their genesis distribution of Zuunbayan subbasin with various drilling, well logging, seismic, coring and outcrop data, aiming at providing significant guidances for the petroleum exploration and production of Zuunbayan area. Therefore, several conclusions have been achieved as follows: ①In Zuunbayan Subbasin, there are two chief source areas with Tarkhyata and Totoshan Uplifts in the southeast and Saykhandulaan Uplift in the west, respectively, while two subsidiary ones in the northeast and southwest of this subbasin. The sedimentary system of alluvial fan-fan delta is formed in the southeast highland, meanwhile braided river-braided river delta develops in the western ramp region and fan delta in the southern palaeohigh. ②There are middle to high permeability reservoirs in the upper Zuunbayan Formation and the upper member of lower Zuunbayan Formation meanwhile low-porosity and permeability to ultra-low permeability ones in Tsagaan Tsav Formation and the middle and lower members of lower Zuunbayan Formation. Combing with sedimentary facies belt, oil sources conditions and tectonic settings, favorable reservoir belts have been proved to be existing in the fan delta front reservoirs of lower Zuunbayan – Tsagaan Tsav Formation in the central uplift faulted zone as well as the braided river front ones of lower Zuunbayan-Tsagaan Tsav Formation in Zuunbayan nose anticlinal structural belts. ③The reservoir lithologic composition is complex and also related to volcanic activities. Generally, the types of lithologic composition in Zuunbayan Subbasin are chiefly feldspathic litharenites with low compositional maturity and high-middle textural maturity. The rock constituents from upper Cretaceous to lower Zuunbayan Formation are mainly metamorphic rocks including cleaving stone, phyllite, quartzite and schist while volcanic tuffs and acidic extrusive rocks are the secondary; and in the Tsagaan Tsav Formation are mainly volcanic tuffs with subsidiary cleaving stone, phyllite, quartzite and schist. ④In this paper, high-quality reservoirs in the upper member of lower Zuunbayan Formation have been discovered in the drilled high production wells of favorable reservoir facies through sedimentary system and sedimentary facies research, which benefits the prospect and also will bring a new life for petroleum exploration and production of Zuunbayan Subbasin. Key words: sedimentary system, sedimentary facies, superior quality reservoir, Zuunbayan Subbasin, lower Zuunbayan Formation