B itte r an d Sw e e t /Um am i Ta s te Re c ep to rs w ith D iffe ren tly Evo lu tio n a ry Pa thw ays

通过生物信息学和系统发育学分析,研究了苦味受体和甜味/鲜味受体的进化途径。结果显示,苦味受体 和甜味/鲜味受体在进化上具有远相关,并且具有不同的进化途径,提示这可能是导致这些受体具有不同功能,传 导不同味觉的原因。

云南保山猪线粒体DNA D loop区序列初步分析

为了解云南保山猪(Baoshan pig)的遗传多样性及其遗传背景，我们测定了19个个体线粒体DNA Dloop高变区1 1 5 363 - 1 5 801片段序列438帅。检测到1。种单倍型，包括8个多态位点，其中5次T/ C转换、1次G/ A转换、1次G/ C颠换和1次A/ T颠换，其A.T.GX碱基的平均含量分别为35.4%.26.9%.13.2%和24.5 %,A+ T含量(62 .3)明显高于G+ C含量(37 .7 %)。对于保山猪的保种及其持续利用有着重要的理论指导意义。

白头叶猴线粒体ND4基因和D-环区的序列及其分类地位初探

用PCR(多聚酶链式反应)扩增方法测定白头叶猴、黑叶猴和菲氏叶猴各1只的线粒体DNA ND4整个基因(1377 bp)和D-环500多bp序列。黑叶猴与菲氏叶猴的ND4基因序列碱基差异数高达123,基于ND4基因的遗传距离值为8.9%;白头叶猴与黑叶猴ND4基因序列间碱基差异数为25,遗传距离值为1.8%。在D-环区域,白头叶猴与黑叶猴间的遗传距离为4.3%,而白头叶猴、黑叶猴两者与菲氏叶猴间的遗传距离则分别高达26.4%和26.0%。分析结果表明,白头叶猴恐怕还只是黑叶猴的一个亚种。从保护遗传学角度看,白头叶猴应被看作一个进化显著性单元(evolutionary significant units, ESU),在动物的保护行动中应得到充分的重视。

鲤属鱼类mtDNA控制区（D-环区）序列的变异性分析

采用PCR-测序技术对我国鲤属7个种、亚种和2个品种共62个个体（其中鲤的样品包括采自长江、黄河、松花江三个水系的共4个种群），进行了mtDNA控制区（D-环区）始自3^端共459bp碱基的序列测定，发现其D-环区3^端至中央保守区之间不似其他鱼类和哺乳类是一个单一的高变区，而是在其内部还插有一段长约110bp的保守区，这很可能是鲤属鱼类mtDNA D-环区自身的一个特点。

5，6-二羟基-2-（4-羟苯基）-4-酮-4H-1-苯并吡喃基-7-羟基-β-D-葡萄糖醛酸在制药中的应用

本发明提供化合物5，6-二羟基-2-(4-羟苯基)-4-酮-4H-1-苯并吡喃基-7-羟基-β-D-葡萄糖醛酸在制备抗艾滋病药物中和在制备逆转录酶抑制剂药物中的应用。

毛蕊异黄酮-7-氧-β-D-吡喃葡萄糖苷或其盐在抗艾滋病中的应用

本发明属于医药技术领域，公开了毛蕊异黄酮-7-氧-β-D-吡喃葡萄糖苷或其盐的新的医药用途。经药理试验证明，毛蕊异黄酮-7-氧-β-D-吡喃葡萄糖苷或其盐具有抗艾滋病毒作用，可治疗艾滋病，可在制备抗艾滋病药物中应用。

猴艾滋病D型逆转录病毒筛选抗艾滋病药物实验模型的建立及应用

以药物对合胞体形成的抑制和对细胞的毒性作为检测指标，应用猴艾滋病D型逆转录病毒（SRV）／Raji细胞系统建立了体外筛选抗艾滋病药物的实验模型。AZT和天花粉蛋白可显著地抑制SRV诱导的合胞体形成，它们的选择指数（SI）分别为数13500和8812。用该模型筛选了46种来源于天然资源的化合物，结果表明有11种天然化合物有明显的抗病毒活性。

检测猴艾滋病D型逆转录病毒抗体的ELISA技术

云南省应用基础研究基盎以及云南夏长娄中心和昆明动物所 长羹生物学旺台实验室基金资助。

SEROLOGICAL SURVEY OF A CAPTIVE MACAQUE COLONY IN CHINA FOR ANTIBODIES TO SIMIAN TYPE-D RETROVIRUSES

Sera from 510 macaques consisting of Macaca mulatta, Macaca assamensis, Macaca fascicularis, Macaca nemestrina, and Macaca arctoides were investigated for antibodies to simian AIDS type D retrovirus (SRV) by ELISA and Western blot with viral antigens purified from supernatants of SRV-1 infected cell cultures. Of these monkeys, 104 were seropositive by ELISA; only 23 were confirmed by Western blot. The true positive reaction to SRV was found in 15 of 463 (3.2%) M. mulatta and eight of eleven (72.7%) M. assamensis.

Sphenalactones A-D, a new class of highly oxygenated trinortriterpenoids from Schisandra sphenanthera

Four novel highly oxygenated trinortriterpenoids, sphenalactones A-D (1-4), were isolated from the leaves and stems of Schisandra sphenanthera and their structures were elucidated by extensive analysis of 1D and 2D NMR data. Compounds 1-4 featured a C-27

Phomoeuphorbins A-D, azaphilones from the fungus Phomopsis euphorbiae

Four azaphilones, named phomoeuphorbins A-D (1-4) were isolated from cultures of Phomopsis euphorbiae, an endophytic fungus isolated from Trewia nudiflora. Structures of 1-4 were established on the basis of spectroscopic analyses, including application of

Periglaucines A-D, anti-HBV and -HIV-1 alkaloids from Pericampylus glaucus

Four new hasubanane-type alkaloids, periglaucines A-D (1-4), and three known alkaloids, norruffscine (5), (-)-8-oxotetrahydropalmatine (6), and (-)-8-oxocanadine (7), were isolated from the aerial parts of Pericampylus glaucus. Their structures were eluci