Antihemostatic molecules from saliva of blood-feeding arthropods

The ability to feed on vertebrate blood has evolved many times in various arthropod clades. Consequently, saliva of blood-feeding arthropods has proven to be a rich source of antihemostatic molecules. A variety of platelet aggregation inhibitors antagonize platelet responses to wound-generated signals, including ADP, thrombin, and collagen. Anticoagulants disrupt elements of both the intrinsic and extrinsic pathways. Vasodilators include nitrophorins (nitric oxide storage and transport heme proteins), a variety of peptides that mimic endogenous vasodilatory neuropeptides, and proteins that catabolize or sequester endogenous vasoconstrictors. Multiple salivary proteins may be directed against each component of hemostasis, resulting in both redundancy and in some cases cooperative interactions between antihemostatic proteins. The complexity and redundancy of saliva ensures an efficient blood meal for the arthropod, but it also provides a diverse array of novel antihemostatic molecules for the pharmacologist.

Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells

To understand better the molecular mechanisms of differential migration of antibody-secreting cells (ASCs) into mouse genital tracts, and regulation by sex hormones, surface markers, hormone receptors and adhesion molecules in mouse SG2 and PA4 hybridoma cells, respectively, secreting IgG2b and polymeric IgA antibody were detected by flow cytometry or RT-PCR. Semiquantitative RT-PCR was also used for measuring mRNA expression of adhesion molecules and chemokines (VCAM-1, ICAM-1, P-selectin, JAM-1 and CXCL12) in genital tracts of various adult mouse groups. The mRNAs of androgen receptor, estrogen receptor beta and CXCR4 were expressed in the ASCs. Sex hormones had no effect on expression of these molecules in ASCs. Except for VCAM-1, mRNA of all examined genes was expressed in normal mouse genital tracts. The mean of relative amounts of ICAM-1 and CXCL12 mRNA in all examined organs of females were higher (2.1- and 1.9-fold) than those in males. After orchiectomy or ovariectomy, the expression of ICAM-1, CXCL12 and P-selectin mRNA in the examined organs increased, except JAM-1 in male and CXCL12 in female. Sex hormone treatment recovered the changes to normal levels of mRNA expression in many examined genital tissues. In combination with our previous work, preferential migration of ASCs into female genital tract and regulation of migration by sex hormones are associated with expression patterns of adhesion molecules and chemokines in genital tract rather than in ASCs. (C) 2006 Elsevier Ireland Ltd. All rights reserved.

HLA-DQB1*0301与胃腺癌及幽门螺杆菌感染的关系

为探讨HLA DQB1等位基因与胃腺癌临床特征及其幽门螺杆菌 (Hp)感染的关联性 ,运用序列特异性引物聚合酶链反应技术 ,检测无亲缘关系湖北汉族健康人 136例、胃癌组 6 3例患者的HLA DQB1基因。内镜活检、Giemsa染色和 (或 )外周血ELISA检查胃粘膜Hp感染情况。SAS软件统计处理。结果表明HLA DQB1 0 30 1与湖北汉族人胃腺癌呈正关联。携带与非携带该等位基因患者 ,其临床特征包括患者平均患病年龄、性别比、肿瘤原发部位、肿瘤TNM分期、肿瘤细胞分化程度 ,以及Hp感染率等情

湖北食管癌HLA-DQB1的基因多态性

目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0

Kinetic study for hopping conduction through deoxyribonucleic acid molecules

Recent experiments indicated that disorder effect in deoxyribonucleic acid (DNA) may lead to a transition of the electronic hole transport mechanism from band resonant tunneling to thermally activated hopping. In this letter, based on Mott's variable-range hopping theory, we present a kinetic study for the hole transport properties of DNA molecules. Beyond the conventional argument in large-scale systems, our numerical study for finite-size DNA molecules reveals a number of unique features for: (i) the current-voltage characteristics, (ii) the temperature and length dependence, and (iii) the transition from conducting to insulating behaviors. (c) 2005 American Institute of Physics.

Electrical transport through individual DNA molecules

A theoretical model is presented to describe electrical transport through individual DNA molecules. By contacting the proposed model with the experimentally measured data, a variety of valuable quantities are identified. The partially decoherent nature on the guanine-cytosine (GC) pairs of DNA is also elaborated in contrast to the completely incoherent hopping mechanism discussed in the context of charge transfer experiments. (C) 2001 American Institute of Physics.

基于HLA卫星组网仿真想定与HLA Web化扩展的研究

卫星组网仿真是对各种卫星组网方案进行性能分析、效能评估以及优化设计的有效途径。限于单机负载及计算能力，卫星组网仿真采用分布式交互仿真形式。作为仿真的基本依据，想定编辑生成与想定发布是仿真首先需要解决的问题。此外，想定推演过程也应对仿真场景进行展示以方便观察仿真当前状态。如何构建满足需求的卫星组网仿真想定系统是本文研究目的之一。 围绕卫星组网仿真想定系统的功能需求，首先进行了需求分析与功能定义，接着对所涉及难点包括多任务多粒度组网仿真想定的灵活配置、细粒度组网仿真内存使用优化以及想定数据实时发布等的技术途径进行了比较与分析。在确定所选用技术基础上，设计出想定系统的总体结构框架。最后结合该框架对系统实现进行了详细的说明，并对内存优化问题进行了仿真实验。 在想定系统实现基础上，进一步分析了当前高层体系结构HLA标准在分布式式交互仿真应用中存有的缺陷，给出利用Web服务对其进行扩展的必要性和扩展途径。通过对面向服务的体系结构SOA及其实现技术Web服务的讨论，分析了通过Web服务扩展HLA使其Web使能的多种途径。文中提出一种基于代理、使用Web服务的HLA Web化扩展方法，并给出了设计方案。

一种基于HLA的卫星仿真系统的设计与实现

针对当前卫星仿真中仿真工具可扩展性差和空间环境模型复杂的问题,设计并实现了一种基于HLA的分布式卫星仿真系统。在架构设计层面,遵循分布式系统设计思想,把系统自底向上划分为数据支撑层、仿真支撑层和仿真应用层,并基于负载均衡的考虑,设计了四个功能集中的仿真应用子系统。在系统实现层面,结合虚拟现实技术,实现了想定方案的灵活配置以及星空环境和在轨卫星的实时渲染,搭建了支持多种卫星仿真应用的通用仿真运行平台。在仿真应用层面,实现了在此平台上对卫星编队变轨过程的仿真,并实时采集仿真数据对仿真结果进行分析和评估。

Continuum structures at the second dissociation limit of hydrogen molecules

We describe near-threshold high-resolution spectra and continuum resonance dynamical behaviour of all three stable hydrogen isotopic variants, and finally obtain improved values for the dissociation energies of hydrogen molecule and its ion. The second dissociation limit is determined by analysing the onset of the vibrational continuum.