Studies on nitric oxide synthase activity in haemocytes of shrimps Fenneropenaeus chinensis and Marsupenaeus japonicus after white spot syndrome virus infection

The nitric oxide synthase (NOS) activity in the haemocytes of shrimps Fenneropenaeus chinensis (Osbeck) and Marsupenaeus japonicus (Bate) was Studied after white spot syndrome virus (WSSV) infection to determine its characteristics in response to virus infection. First, the NOS activity in haemocytes of shrimps was determined by the means of NBT reduction and changes in cell conformation. And the variations of NOS activity in shrimps after challenge with WSSV intramuscularly were evaluated through the analysis Of L-citrulline and total nitrite/nitrate (both as NO derivates) concentrations. The result showed that NOS activity in the haemocytes of F chinensis increased slightly from 0 to 12 h postchallenge, indicated by the variations Of L-Citrulline (from 11.15 +/- 0.10 to 12.08 +/- 0.64 mu M) and total nitrite/nitrate concentrations (from 10.45 +/- 0.65 to 12.67 +/- 0.52 mu M). Then it decreased sharply till the end of the experiment (84 h postchallenge), the concentrations Of L-Citrulline and total nitrite/nitrate at 84 It were 1.58 +/- 0.24 and 2.69 +/- 0.70 mu M, respectively. The LPS-stimulated NOS activity kept constant during the experiment. However, in M. japonicus, the NOS activity kept increasing during the first 72 It postchallenge, the concentrations Of L-Citrulline and total nitrite/nitrate increased from 7.82 +/- 0.77 at 0 h to 10.79 +/- 0.50 mu M at 72 h, and from 8.98 +/- 0.43 at 0 h to 11.20 +/- 0.37 mu M at 72 h, respectively. Then it decreased till the end of the experiment (216 h postchallenge), and the concentrations of L-Citrulline and total nitrite/nitrate at 216 h were 5.66 +/- 0.27 and 4.68 +/- 0.16 mu M, respectively. More importantly, an apparent increase of I-PS-stimulated NOS activity was observed in M japonicus at 48 h postchallenge, which was about 4 times higher than that in the control group of health shrimps. In correspondence with the difference of NOS activity between the two species of shrimps, the Cumulative mortalities of the shrimps were also different. All shrimps of F. chinensis in the mortality experiment died in 66 h, much more quickly than M. japonicus, Whose accumulative mortality reached 100% after 240 h. Data here reported let us hypothesize that NOS activity in the haemocytes of shrimps F chinensis and M. japonicus responses to WSSV infection differently, and this might be one of the reasons for the different susceptibility of F chinensis and M. japonicus to WSSV infection. (c) 2005 Elsevier Inc. All rights reserved.

The effect of acute salinity change on white spot syndrome (WSS) outbreaks in Fenneropenaeus chinensis

In order to observe the effect of salinity on disease resistance and white spot syndrome virus (WSSV) proliferation in Fenneropenaeus chinensis, shrimps with latent WSSV were subjected to two acute salinity changes from the original salinity of 22 ppt to 18 and 14 ppt in an hour, respectively. The total haemocyte count (THC) of the challenged group showed no evident change under salinity adjustments, but the phenoloxidase (PO) index declined significantly (P<0.05) corresponding to continuing acute salinity changes from the 24th to the 72nd hour. According to the WSSV load detected by quantitative real-time PCR method, it was found that WSSV carried by the challenged group and control group were significantly different (P<0.05); acute salinity change from 22 to 14 ppt led to the WSSV carried in the challenged group being significantly higher (P<0.05) than that of those surviving in 22 ppt, but salinity change from 22 to 18 ppt had no such effect. At the end of the 72-h experiment, the challenged group subjected to salinity change from 22 to 14 ppt had nearly 3 times the WSSV load as the control group with no salinity change. Therefore, salinity changes over a particular range could result in a decrease of immunocompetence and obvious WSSV proliferation in the shrimps, leading to white spot syndrome developing from a latent infection to an acute outbreak. (C) 2005 Elsevier B.V All rights reserved.

基于ARM的研磨抛光机器人运动控制器的设计

本文设计了研磨抛光机器人分布式控制系统中的一种运动控制器,并对运动控制器基于AT91M40800微控制器的硬件结构、基于μC/OS-Ⅱ实时操作系统的软件模块和采用的参数模糊自整定PID机器人关节位置控制策略进行了详细介绍。实验表明该控制器可以大大降低研磨抛光机器人的位置跟踪误差。提高了关节控制的计算及处理能力,易于扩展和维护。

基于ARM的可编程控制系统实现

采用ARM设计小型可编程控制器,提供基于以太网和OPC标准的组态监控接口。用户采用符合IEC61131-3标准的梯形图方式对控制器自由编程,并通过计算机网络对其进行程序调试、下载和数据通信,从而实现了一套经济实用的小型可编程控制系统。

基于ARM的智能型定氮仪的研制

通过对凯氏定氮法和整个系统控制原理的分析,介绍了一种基于ARM的测定物质中氮元素含量的系统,该系统采用ARM微控制器LPC2214作为整个系统的控制核心,通过步进电机驱动进行标准溶液的滴定和光电检测确定滴定终点,形成闭环系统,实现了准确测定物质中氮元素的含量。文中给出了系统软硬件设计方案,对其实现方法及关键技术进行了研究。实验结果表明:该系统工作稳定,测量数据准确,具有一定的应用价值。

ARM处理器在AFM纳米操作系统中的应用

文章介绍了在原子力显微镜(AFM)纳米操作系统中ARM处理器的应用及开发,在此基础上搭建了具有3D力反馈的纳米操作系统。结合AFM纳米操作系统的实时性问题,进行了ARM开发板的系统软件架构和PSD(四象限光电检测器)信号的PID控制结果的分析,使纳米操作系统在实时性和成像的质量等方面都得到相应改进。

ARM嵌入式系统在纳米操作系统中的应用研究

近年来，随着微纳米科技的迅速发展，机电产品有望向更微观化、高性能化发展，这将促进材料、制造、电子、生物医学、信息等领域新的科学技术出现，在新的科学技术层次上为可持续发展的理论提供物质和技术保障。微纳米科技最终目标是研究和发现微纳尺度物质所具有的新颖的物理、化学和生物学现象与特性。并以此为基础来设计、制作、组装成新的材料、组件或系统，实现与之相应的特定功能，促进新的科学技术发展与变革，这无疑具有十分重要的科学意义和经济价值。而实现这个目标的使能技术便是微纳米尺度下观测、操作和装配的科学方法与相关的技术和装备，因此开展微纳米操作研究具有特别重要的意义。微纳米操作是微纳米制造科学技术的重要内容之一，使用探针模式的机器人化微纳操作方法，实现在微纳米尺度物体的可控操作，对促进我国微纳米科学技术发展具有特别重要的意义。 目前已有的基于探针的纳米技术装置如SPM (Scanning Probe Microscope)是基于探针模式的纳米观测基本装置。在此基础上研究发展的基于探针的纳米操作已成为纳米科技研究的新领域，是目前世界上各国正在大力开发的前沿研究课题。但目前市场上的SPM等纳米观测设备缺乏驱动控制与信息交互功能和开放界面，限制了用户在此基础上开发纳米操作、装配等功能的能力，因而研究具有信息交互能力的、可进行在线操作控制与宏-微-纳观信息交互的纳米操作监控系统，进而发展成具有自动化/机器人化功能的纳米作业系统队纳米科学技术发展、纳米制造的实现无疑具有重要意义。本论文的科研内容是以面向纳米制造的机器人化系统为研究背景，在自主技术的基础上，开展应用ARM嵌入式系统构成纳米作业系统的实时控制器研究。实时多任务的操作控制系统是纳米作业系统的核心技术，可以实时进行基于探针的传感信息采集、状态反馈控制、形貌观测数据生成、作业运动轨迹生成、位置反馈控制等功能的数据处理与实现。本论文重点介绍以SAMSUNG公司的ARM9处理器芯片S3C2410为嵌入式控制器系统的核心，在移植嵌入式Linux作为操作系统的基础上，开发具有实时数据采集与控制指令、通信功能的人机交互界面。基于ARM的实时控制器的研究为探针模式的纳米观测与操作系统开发提供了关键技术，可以提供开放的AFM系统，促进操作型纳米系统的研究与实现，可以保证纳米观测与操作控制的实时性，可以为纳米作业控制方法提供方便的编程、开发功能。本论文主要研究了面向纳米作业的基于ARM嵌入式实时控制器硬件结构及软件系统的研究与开发过程。首先介绍嵌入式系统的基本概念和特点；其次介绍基于SPM模式的纳米操作系统性能与技术特点；第三，根据纳米作业系统的技术功能要求，详细介绍了具有实时多任务管理功能的硬件系统的设计，重点解决核心板和扩展板各部分功能模块的设计；第四，详细介绍了嵌入式Linux操作系统下的应用程序开发模式及开发过程；最后，详细介绍了嵌入式Linux操作系统下的应用程序开发，主要工作是完成SPM纳米操作系统中的ARM开发平台的功能接口模块的调试及Linux系统下多线程技术在本系统中的应用。本次毕业设计已完成ARM开发平台在整个SPM纳米操作系统中要实现的各个功能模块，结合SPM纳米操作系统的实时性问题，进行了ARM开发平台的系统软件架构分析和利用多线程技术的以太网通信实验，在一定程度上提高了纳米操作系统中的实时性和成像质量。