生姜化感作用机理及其栽培模式研究

近年来，随着对作物重茬（连年种植）障碍原因的深入研究，植物的化感作用越来越受到国内外众多学者的重视。而作为重要调料和药用植物的生姜，其连作障碍也备受关注，系统地研究生姜化感作用将有助于理解和最终解决生姜连作障碍问题。本文通过研究生姜不同部位、不同浓度的水浸液对与其间作的两个物种（大豆和四季葱）种子的萌发及幼苗生长的影响，从而证明生姜化感作用的存在；并通过温室盆栽实验研究了生姜的自毒作用（即研究生姜不同部位、不同浓度的水浸液对其幼苗的形态、生理生化、光合作用、土壤酶、土壤微生物多样性及土壤养分的影响），从而揭示生姜退化和衰老的机制，并为生姜筛选出合适的间作物种提供科学依据，对生姜连作障碍提出科学的解决方法。主要研究结果如下： 1. 与对照相比，生姜所有部位（根茎、茎、叶）、所有浓度（10、20、40、 80 g l-1）的水浸液均抑制了大豆种子和葱籽的萌发率、幼苗生长、水分吸收和脂肪酶活性，并且其抑制程度随着水浸液浓度的增加而增强，其生姜各部位水浸液抑制效应的强弱顺序为茎＞叶＞根茎。这一结果表明生姜根茎、茎、叶含有能够抑制大豆种子和葱籽种子萌发和幼苗生长的水溶性化感物质。根茎是生姜的主要收获部位，而生姜的残株（主要是茎和叶）应该从大田中处理掉以减轻其抑制效应。生姜水浸液中主要化感成分包括：根茎水浸液中主要是丁香酸和伞花内脂；茎水浸液中主要是阿魏酸，且其含量最高为73.4 ug/g；叶水浸液中除了阿魏酸，其他六种物质均检测出来，但含量较高的主要有丁香酸、伞花内脂和香豆酸。 2. 生姜茎和叶不同浓度的水浸液均显著抑制了生姜幼苗的株高、每株叶片数和叶面积，其抑制程度随着水浸液浓度的增加而有所增强，而生姜幼苗每株分枝数差异不显著；同时生姜水浸液也极大程度地影响了生姜幼苗的生物量（包括地下生物量、地上生物量和总生物量，均为鲜重）。在同一浓度下，茎水浸液对生姜幼苗形态指标及生物量指标均显示出最强的抑制作用，叶水浸液次之，根茎水浸液最弱。与对照相比，低浓度的生姜根茎水浸液提高了生姜幼苗叶片内四种抗氧化酶（SOD、POD、CAT、APX）活性，高浓度的根茎水浸液抑制了四种抗氧化酶活性，而茎和叶水浸液均随着浓度的增加而抑制了四种抗氧化酶活性，三种水浸液均随着浓度的增加降低了生姜幼苗叶片内叶绿素的含量，而增加了生姜幼苗叶片的相对电导率和丙二醛含量。同时，三种水浸液均随着浓度的增加降低了生姜幼苗的光合参数（包括胞间CO2浓度、气孔导度、蒸腾速率及净光合速率）。 3. 三种生姜水浸液对所测六种土壤酶活性均产生了不同程度的影响，其中影响最大的是酸性磷酸酶和蔗糖酶，在10 g l-1 时就达到了显著水平，并且所有酶均有随着水浸液浓度增加而增大的趋势；相同部位的水浸液随着浓度的增加，细菌和真菌的数量呈增加趋势，而放线菌的数量呈减少趋势；三种生姜水浸液均随着浓度的增加降低了土壤中有机质的含量，加剧了土壤中硝态氮含量的积累，根茎水浸液对土壤有效磷、速效钾和铵态氮均显示出低浓度提高其含量而高浓度降低其含量的趋势，而茎和叶水浸液则随着浓度的增加均降低了其含量。 4. 与生姜单作相比，所有间作系统均在旺盛生长期和收获期不同程度地提高了土壤酶活性，同时也增加了土壤细菌数量及土壤微生物总数但不显著；所有间作系统在旺盛生长期和收获期均不同程度地影响了土壤真菌及放线菌数量（增加或减少），所有间作系统间的多样性指数差异不显著，除了旺盛生长期四种作物（生姜-大豆-四季葱-大蒜）的间作模式显著降低了多样性指数，其值仅为生姜单作的33.18%；生姜与大豆间作不仅提高了19.6%的生姜产量而且获得了较好的经济效益，并且，所有间作系统均显著抑制了生姜姜瘟病的发生。 5. 不同栽培模式不同程度地影响了收获期生姜的株高、分枝数、根茎产量及内在品质。其中处理2显著地促进了生姜的分枝（10.5%），同时处理2、3和4也促进了生姜的生长（株高分别增加了15.0%、11.4%和14.0%），并且这三个处理提高了生姜的产量；处理2和3能有效提高生姜块茎中维生素C（分别较单作生姜显著提高了3.29%和4.05%）、处理3显著提高了可溶性糖（8.2%）、姜辣素（4.6%）和蛋白质等有益物质的含量，降低硝酸盐有害物质的含量（处理2显著降低了14.0%），改善了姜块的外观和内在品质。并且，生姜与大豆间作具有最高的纯收入和产投比，分别较生姜单作提高了24.80%和8.8%。Recently, allelopathy has been more and more paid attentions by national and foreign scholars with profound research on reasons of crop replanted (continuous planted) obstacle. Ginger rhizome is valuable all over the world either as a spice or herbal medicine and ginger replanted obstacle is also paid attentions. Systematic research on ginger allelopathy will contribute to understanding and ultimate solving problem of ginger replanted obstacle. The effects of ginger aqueous extracts with different parts and concentrations on seed germination and early seedling growth of soybean and chive were studied in this article to testify that ginger existed allelopathy. Furthermore, ginger autotoxicity was also studied by pot experiment in greenhouse (namely research on effects of ginger aqueous extracts with different parts and concentrations on morphological indexes, physiological and biochemical indexes, photosynthesis, soil enzymes, soil microbial diversity and soil nutrients) to reveal mechanism of ginger degeneration and senescence, provide scientific basis for selecting appropriate intercropping species and put forward scientific resolvent for ginger replanted obstacle. The main results were as follows: 1. All aqueous extracts at all concentrations inhibited seed germination, seedling growth, water uptake and lipase activity of soybean and chive compared with the control, and the degree of inhibition increased with the incremental extracts concentration. The degree of toxicity of different ginger plant parts can be classified in order of decreasing inhibition as stem＞leaf＞rhizome. The results of this study suggested that rhizome, stem and leaf of ginger contained water soluble allelochemicals which could inhibit seed germination and seedling growth of soybean and chive. The rhizome is the main harvested part of ginger. The residue (mainly stems and leaves) of the ginger plant should be removed from the field so as to diminish its inhibitory effect. The main allelopathic components of three kind of aqueous extracts were as follows: Rhizome extract chiefly contained syringic acid and vmbelliferone and stem extract mainly contained frulic acid whose content was the highest (73.4 ug/g). The other six substances were detected except of frulic acid, but only contents of syringic acid, vmbelliferone and p-coumaric acid were higher. 2. Stem and leaf aqueous extracts of ginger with different concentrations significantly inhibited plant height, leaf numbers per plant and leaf area, and the degree of inhibition increased with the incremental extracts concentration. However, tiller number per plant of ginger seedling showed no significant difference. At the same time, ginger aqueous extracts also influenced biomass including under-ground biomass, above-ground biomass and total biomass (fresh weight) to a large extent. Under the same concentration, stem aqueous extract showed the mostly inhibitory effect on morphological indexes and biomass indexes of ginger seedling. Rhizome aqueous extract showed the leastly inhibitory effect and leaf aqueous extract was intervenient. Enhanced concentration of ginger aqueous extracts significantly reduced total chlorophyll content, accompanying with increases in memberane permeability (REL) and lipid peroxidation (MDA). Compared with the control, rhizome ginger aqueous extract of lower concentration (10 g l-1) increased the activities of major antioxidant enzymes (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; ascorbate peroxidase, APX) of ginger leaf tissue and higher concentration inhibited the activities of four antioxidant enzymes. However, stem and leaf aqueous extract inhibited the activities of four antioxidant enzymes with increase in concentration. Meanwhile, enhanced concentration of ginger aqueous extracts significantly reduced photo-parameters of ginger seedling (including CO2 concentration, stoma conductivity, net photosynthesis rate and transpiration rate). 3. Rhizome, stem and leaf ginger aqueous extract showed different effect on six soil enzyme activities, and acid phosphatase and invertase showed significant effect when aqueous extract concentration got 10 g l-1. Furthermore, six soil enzyme activities increased with increase in aqueous extract concentration. Bcterial and fungi number tended to increase while antinomyces tented to decrease with the increase in aqueous extract concentration of identical part. Ginger aqueous extracts reduced soil organic matter content with increased concentration, accompanying with NO3-—N accumulation in soil. Rhizome aqueous extract showed the same tendency for available P, available K and NH4+—N, namely lower concentration increased their contents in soil and higher concentration reduced their contents. While stem and leaf aqueous extracts reduced their contents with the increamental concentration. 4. All intercropping systems increased soil enzyme activities to different extent both at VGS and at HS compared to solo ginger. All intercropping systems increased the colony numbers of soil bacteria and total of soil microbe but not significantly either at VGS or at HS. All intercropping systems increased the colony numbers of soil fungi and actinomytes to a different extent (increase or decrease) both at VGS and at HS. For DI, difference between all cultivation patterns and S-G was not significant either at VGS or at HS except that G-S-C-G whose value was only 33.18% of S-G at VGS significantly decreased. G-S not only increased ginger yield by 19.6% but also obtained better economic benefit. Furthermore, all intercropping systems significantly inhibited occurrence of bacterial wilt of ginger. 5. Different cultivated pattern influenced plant height, tiller numbers, rhizome yields and intrinsic quality of ginger. Treatment 2 significantly facilitated tiller occurring (10.5%). Treatment 2, 3 and 4 promoted ginger growth (plant height respectively increased 15.0%、11.4% and 14.0%) and enhanced rhizome yields. Treatment 2 and 3 effectively increased vitamin C content (significantly increased 3.29% and 4.05% compared to solo ginger). Treatment 3 significantly increased contents of beneficial substances such as soluble sugar (8.2%), gingerols (4.6%) and protein. Treatment 2 significantly decreased contents of deleterious substance namely nitrate (14.0%) and improved appearance and intrinsic quality of ginger rhizome. Furthermore, treatment 2 (ginger/soybean intercropping) could obtain better economic benefit and showed the highest net income and ratio of benefit and cost whose values respectively increased by 24.80% and 8.8% compared to solo ginger.

酿酒酵母和运动发酵单胞菌木薯乙醇发酵条件研究

本文结合我国燃料乙醇发展的方针政策，以酿酒酵母和运动发酵单胞菌为菌种研究其在非粮能源作物木薯中乙醇发酵的情况，为木薯原料更好地应用于生产中提供了理论依据。 酿酒酵母木薯高浓度乙醇发酵的研究。实验采用的木薯干淀粉含量约70-75%。以酿酒酵母为菌种进行高浓度乙醇发酵的工艺条件研究，最佳条件为：木薯干粉碎细度为35目，料水比1：2，α-淀粉酶用量0.09 KNU/g淀粉，蒸煮温度85 ℃，蒸煮时间15 min。采用30 ℃同步糖化发酵工艺，糖化酶用量为3.4 AGU/g淀粉，发酵时间30 h。在10 L发酵罐中，乙醇质量比达127.88 g/kg，发酵效率为88.28%，发酵强度4.263 g/kg/h，100 L中试研究中乙醇浓度为127.75 g/kg，发酵强度4.258 g/kg/h。利用高效液相色谱对发酵液中残糖进行了分析，证明葡萄糖、果糖等单糖已完全被菌体利用，剩余糖为二糖，三糖等不可发酵的低聚糖。 运动发酵单胞菌快速乙醇发酵的研究。对实验室保藏的8株运动发酵单胞菌进行比较，选择发酵速度最快的Zymomonas mobilis232B进行研究。该菌在纯葡萄糖中的最佳发酵条件为：葡萄糖浓度18%，起始pH 6-7，发酵温度30 ℃，发酵时间18 h，乙醇浓度88 g/kg。在以木薯为底物同步糖化快速乙醇发酵中，采用Full Factorial设计和最速上升实验确定了培养基成分中的2个显著性因子及其最适浓度：酵母粉4 g/kg，硫酸铵0.8 g/kg。在最适培养基条件下，对木薯料水比和糖化酶用量进行了优化，得到Z.mobilis232B木薯乙醇发酵最佳料水比1：3，糖化酶浓度4 AGU/g淀粉，乙醇发酵4.915 g/kg/h。利用高效液相色谱对发酵液中残糖进行了分析，剩余糖为二糖，三糖等，但成分较酵母发酵后复杂。 According to the fuel ethanol development plans and policies in our country, the ethanol production from cassava by Saccharomyces cerevisiae and Zymomonas mobilis was studied. It provided theoretical basis for ethanol fermentation by cassava in industry. Part 1 is the study of VHG (very high gravity) ethanol fermentation by Saccharomyces cerevisiae. The content of starch in cassava was 70-75%. Compared with the performances under different experimental conditions, the following optimal conditions for VHG fermentation were obtained: Granule size of dry cassava 35 mashes, hydromodulus of cassava to water at 1:2, α-amylase enzyme dosage 0.09 KNU/g starch, cooking temperature 85 ℃ for 15 min, using the SSF process (simultaneous saccharification and fermentation) and the amount of glucoamylase 3.4 AGU/g starch. Accordingly, the final ethanol concentration was up to 127.88 g/kg; the ethanol yield reached 88.28%, and ethanol productivity was 4.263 g/kg/h after 30 h. When the fermentation scale expanded to 100 L, the final ethanol concentration was 127.75 g/kg, and the ethanol productivity was 4.258 g/kg/h in 30 h. The residual sugar was analyzed by high performance liquid chromatography, and proved that there was no glucose and fructose. The residual reducing sugar was some unfermentable oligosaccharide Part 2 is the study of the rapid ethanol production by Zymomonas mobilis. Compare with other seven stains, Zymomonas mobilis 232B was selected for research. The optimum condition in glucose medium was as follow: glucose concentration 18%, initial pH 6-7, and fermentation temperature 30 ℃. The ethanol concentration was 88g/kg in 18 h. After that, rapid ethanol production from cassava in SSF by Zymomonas mobilis 232B was studied. Through a series of experiments aided by Full Factorial Design and steepest ascent search, the optimal concentration yeast extract and ammonium sulfate were determined: 4 g/kg and 0.8 g/kg, each. Under optimum medium conditions, the optimal hydromodulus of cassava to water and glucoamylase dosages were obtained: hydromodulus of cassava to water at 1:3 and glucoamylase dosages 4 AGU/g starch. The ethanol production reached 4.915 g/kg/h. The residual sugar was analyzed by HPLC, and proved that the residual reducing sugar was some unfermentable oligosaccharide，but the components were more complex than that fermentation by Saccharomyces cerevisiae.

LFX-20型逆燃式医疗废物焚烧炉二恶英实测分析

概述我国目前医疗废物焚烧处置中的主要炉型,着重介绍LFX-20型逆燃式焚烧炉焚烧处置医疗废物的实际运行工况。在1燃室、2燃室平均温度分别为869.5℃和898.7℃条件下,对医疗废物焚烧烟气进行检测。各项常规指标均低于国标限值,二恶英检测中其质量浓度实测值为15ng/m3,相应毒性当量为0.46ngTEQ/m3,同时对医疗废物焚烧后产生烟气中二恶英和灰渣的检测结果进行了分析,焚烧后底渣体积约为焚烧前医疗废物体积的1/10,重量为原来的12%～18%,飞灰热灼减率为34.6%,并给出分析结果。

长白山阔叶红松林昆虫多样性研究

通过季节性观察 ,系统地研究了长白山阔叶红松林昆虫类群及其多样性 .结果表明 ,长白山阔叶红松林已知的森林昆虫 2 6目 131科 116 2属 196 0种 ,其中森林害虫 11目 10 5科 881种、重要森林害虫6 38种 ;森林昆虫群落中植食性昆虫类群总数所占比重最大 ,天敌昆虫群落中以捕食性类群总数所占比重最大 .植食类群、寄生性类群和捕食类群全年的均匀度指数分别为 0 884、0 830和 0 80 6 .各类群间季节变动系数的大小顺序为捕食类群 >寄生性类群 >植食类群 .

辽东山区天然次生林雪/风灾害成因及分析

对 2 0 0 3年春发生在辽东山区的森林雪 /风灾害的成因、过程、受灾情况、造成的危害、与林分结构特征的关系以及对未来次生林生态系统的影响等进行了调查分析 .结果表明 ,雪 /风的发生是在一个大的降水天气过程基础上 ,由于气温的异常变化形成的 .受灾严重区多分布于海拔高、坡度大 ,林型比较单一的桦树、柞树、色树、胡桃楸和杨树等林分 .林分密度和受灾率及土层厚度和受害株数均呈显著的线性负相关 ;受灾数量与径级和树高分别呈指数负相关和指数正相关 .同时探讨了雪 /风害对天然次生林生态系统内病虫害发生、林下植被、生境因子和建群种变化产生的可能影响

辽宁西部低山丘陵区植被恢复研究:基于演替理论和生态系统退化程度

在对辽宁西部地区植被演替分析的基础上 ,选用不同演替阶段的生境指标 ,应用聚类分析方法 ,定量确定了辽宁西部低山丘陵区退化生态系统的退化程度 .在辽宁西部地区 ,南刘杖子林场的天然栎林、天然油松林、杨栎林、牛河梁和欺天林场坡地的人工油松林与天然松栎林的平均相异系数分别为 0 6 5 5、0 6 6 5、2 0 2 9、3 919,综合反映了其在生态系统演替 /退化中的相对位置 .研究表明 ,辽宁西部低山丘陵区现有油松林 (阴坡 )立地大多已退化到灌木林和先锋乔木林之间的演替阶段 ,并且接近于先锋乔木林阶段 ;建议在现有人工林经营改造或营造混交林时 ,应适当增大灌木树种和先锋乔木树种的比重 .

缺磷条件下低分子量有机酸对大豆氮积累和结瘤固氮的影响

采用砂培方法在温室条件下研究了低分子量有机酸柠檬酸、草酸、苹果酸及3种酸的混合物对大豆植株氮素积累、结瘤和固氮的影响.结果表明:低分子量有机酸对大豆植株氮素积累有显著的抑制作用,使大豆地上部各时期氮素积累量的降低幅度分别为:苗期17.6%~44.9%,花期29.8%~88.4%,鼓粒期9.18%~69.6%,成熟期2.21%~41.7%;低分子量有机酸对大豆根瘤生长和固氮能力也有显著影响,表现为使根瘤数量、根瘤固氮酶活性和豆血红蛋白含量显著降低,降低幅度分别为11.4%~59.6%,80.5%~91.7%和11.9%~59.9%,从而使大豆的固氮效率降低,最终导致大豆的固氮量较对照显著降低(降低幅度9.71%~64.5%).低分子量有机酸对大豆氮积累、根瘤生长和固氮能力的抑制作用随浓度的增加而增加.3种有机酸中,草酸的抑制作用相对大于柠檬酸和苹果酸,3种有机酸混合后,抑制作用加强.

沈阳城市森林主要绿化树种——丁香的光合特性研究

应用LI 6 4 0 0红外气体分析仪对城市森林典型树种丁香不同季节光合作用的日变化规律进行研究 ,采用相关分析和多元逐步回归统计分析方法 ,得出净光合速率与光合有效辐射、气温、大气湿度、气孔导度等生理生态因子间的相关关系 .结果表明 ,丁香春季和夏季光合速率呈双峰曲线 ,秋季呈单峰曲线 .春季影响光合速率的主要因子为光合有效辐射和气孔导度 ;夏季影响光合速率的主要因子为气孔导度和光合有效辐射 ;秋季为气孔导度和胞间CO2 浓度 .建立了相应的影响因子回归方程

树冠结构参数及附近风场特征的风洞模拟研究

根据风洞模型实验 ,分析了树冠结构参数 (疏透度 β、透风系数α)和附近的风速场特征 .结果表明 ,透风系数与疏透度之间符合幂函数关系α =β0 .6,树冠附近的风速减弱区为椭球形立体空间 ,减弱区随树高、冠幅的增大而增大 ,随透风系数 (或疏透度 )的增大而减小 ,在水平和垂直剖面上 ,等风速线分别为椭圆形和椭圆线段

土壤温度和湿度对长白松林土壤呼吸速率的影响

2003年6月17日、8月5日和10月10日,研究了长白山长白松林地内土壤呼吸速率和断根土壤呼吸速率日变化,并于2004年5~9月对其季节变化进行了测定.结果表明,土壤总呼吸速率和断根土壤呼吸速率的日变化均呈单峰型,峰值一般出现在12:00~14:00,8月份土壤呼吸速率的日变化幅度小于6月份和10月份.土壤总呼吸速率、断根土壤呼吸速率和根系呼吸速率具有明显的季节变化,6~8月份较高,5月份和9月份较低.2004年5~9月份,土壤总呼吸速率、断根土壤呼吸速率和根系呼吸速率的平均值分别为3.12、1.94和1.18μmolCO2.m-2.s-1,根系呼吸对土壤总呼吸的贡献为26.5%~52.6%.土壤呼吸速率与土壤温度之间呈显著的指数相关,与土壤湿度之间呈线性相关.土壤总呼吸速率、断根土壤呼吸速率和根系呼吸速率的Q10值分别为2.44、2.55和2.27,断根土壤呼吸速率对温度的敏感程度大于土壤总呼吸速率和根系呼吸速率.土壤总呼吸速率对土壤湿度的敏感程度大于根系呼吸,断根土壤呼吸速率对土壤湿度的敏感程度最差.

SYNTHESES OF METHYLCYCLOPENTADIENYL DERIVATIVES OF LANTHANIDES (LN = LA-ND) AND CRYSTAL-STRUCTURES OF [(THF)2LI(MU-CL)2]2[MECPND(THF)] AND [LI(DME)3][MECPLA(NPH2)3]

The reaction of LnCl3.2LiCl with 1 equiv of MeCpNa in THF gives the complexes [(THF)2Li(mu-Cl)2]2[MeCpLn(THF)] (Ln = Nd (1), La (2)) in good yield. These precursors react further with 2 equiv of LiNPh2 to produce the new complexes [Li(DME)3][MeCpLn(NPh2)3] (Ln = La (3), Pr (4), Nd (5)). They have been characterized by elemental analyses and IR and NMR spectra, as well as by structural analyses of 1 and 3. The chloride 1 crystallizes in the monoclinic space group P2(1)/n (No. 14) with a = 12.130 (5) angstrom, b = 17.343 (5) angstrom, c = 17.016 (5) angstrom, beta = 108.54 (3)-degrees, V = 3393.87 angstrom3, Z = 4, and D(c) = 1.45 g/cm3. Least-squares refinement led to a final R value of 0.051 (I greater-than-or-equal-to 3-sigma(I(o))) for 2004 independent reflections. Complex 3 crystallizes in the monoclinic space group P2(1)/c (No. 14) with a = 18.335 (6) angstrom, b = 16.576 (5) angstrom, c = 17.461 (6) angstrom, beta = 96.04 (3)-degrees, V = 5277.17 angstrom3, D(c) = 1.26 g/cm3, Z = 4, and R = 0.057 (I greater-than-or-equal-to 2.5-sigma(I(o))) for 3378 reflections. The structure of 3 consists of discrete ion pairs [Li(DME)3]+ and [MeCpLa(NPh2)3]- with average La-N and La-C(ring) distances of 2.459 (8) and 2.84 (1) angstrom, respectively.

氯化稀土冠醚配合物的电子结构和化学键

合成了LnCl_3·L(Ln=La、Pr、Nd和Sm。L=15-C-5、18-C-6)系列配合物,用XPS和量子化学计算研究了它们的分子构型、电子结构和化学键性质。计算与实验结果一致。在LnCl_3·15-C-5中三个Cl在Ln的一侧,形成8配位配合物。LnCl_3·15-C-5在空气中容易潮解。高层占据分子轨道由Cl 3P和O2P-组成,低层未占据分子轨道由Ln的原子轨道组成。

四株海洋放线菌次级代谢产物的研究

本课题组自1999年以来，将培养条件优化、生物活性跟踪及化学跟踪技术应用到胶州湾海洋放线菌的次级代谢产物研究中，发现了一批具有生物活性的化合物，包括新型骨架等新颖结构的化合物。 本研究从海州湾分离出海洋放线菌172株，对其中70株菌的次级代谢产物进行了生物活性筛选和化学筛选，获得了它们对八种病原微生物的抑制活性数据。发现海州湾海洋放线菌对至少一种受试微生物具有拮抗能力的比例约为30%。从海洲湾分离到的海洋放线菌中筛选得到三株L083、L078和L158用于次级代谢产物的研究，同时本人又从合作实验室获得另一株海洋放线菌B7651，从这四株海洋细菌的发酵粗提物中共分离纯化得到26 个化合物， 其中10 个为新颖结构化合物。具体是，3-Hydroxy-6-[(Z)-3´-hydroxy-2´,4´-dimethyl-hept-4´-enoylamino]-2,4-dimethyl-5-oxo-hexanoic acid (4)，2-[5-(2-oxopropyl)tetrahydrofuran-2-yl]propanoic acid (5)，2-oxatricyclo-octane (10)，Huaiomycin (15)， 5-(6-hydroxy-6-methylheptyl)dihydrofuran-2(3H)-one (17), 6-Hydroxy-6-hydroxymethyl-6H-pyran-3-one (18), 1,6-dihydroxy-hex-3-ene-2,5—dione (19) (1’R, 2R, 4R)-2-(1-hydroxy-8-methylnonyl)-4-hydroxymethyl-butanolide (20) ， Bremeromycin A (22) ，Bremeromycin B (26)。生物活性实验结果表明Bremeromycin A (22)具有选择性的抗枯草杆菌(Bacillus subtilis ATCC 6051)活性和抗微藻Chlorella vulgaris活性。