119 resultados para CU2


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(Agrobacterium rhizogenes)16011000150015834A4(Artemisia annua L.)pRi1601pRi15834pRiA4pRi1601pRi15834;;(Gtowth RatioGR)(Number of Side RootsNSR81601-L-1, 1601-L-2, 1601-L-3, 1601-L-4, 15834-L-1, 1601-P-I, 16 01-P-215834-L-2Southern160l-1-11801-L-2, 1601-L-31601-L-41601-P-11601-P-28QHS(16/8hrs)2516 01-L-11601-L-21601-L-31601-L-41601-P-l1601-P-216 01-L-3160l-L-1;1601-L-1QHS1. 048%1601-1-3QHS160Z-L-315834 -L-12583:1-L-2l000mLMS3000mL1601-L -315834-L-115834-L-2ll0rlpmHairy Root BalisHRBHRBQHsHpLCQHS MSQHS lKN0318.7910-3M1601- L-114. 8410-3MQHSNH-4N0-310.93-12. 49103M1601-L-10-20.6210-3MQHS20. 62lO-3MQHSNH-4+N0-3-0. 37-0. 4-0.52:10.52 - 0.58:1QHS 2H-2P0-4-2.49810-3M0-2. 498l0-3MH2P4 -0-1.249lO-3MQHS1.24910-3MQHS 316 01-L-1Ca-2+0.198- 0.76610-3M0-3.69510-3MCa-2+QHSCa-2+3.695l0-3M 4Mg-2+0. 14210-3M-7.506l0-3MHPLCUVQHSMg-2+QHS 5Fe-2+0. 25 -1010-3M16 01- L-1QHS 616 01- L-3KI2.5ppmKIQHS 7H2BO3l601-L-lHPLCQHSH3BO3100ppm400ppmQHS1.69mg/g1.80mg/g(DW) 8Cu-2+1601-L-31601-L-3Cu-2+1.00ppm0 -1.00ppmCu+QHSCu2+0.05ppmQHS 1601-L-l2535 1601-L-31601-L-1 (1) (2)530- 60glL50glL60g/L30gL (3)1560g/L3060-90g/L30-40g/L (4)51530g/L60g/L90g/L3060g/L90g/L (5)/ pHQHSpH5.O-6.51601-L-15.OpH5.81601-1-1QHSpH4.5-5.2. pH7.OpHl0.OpH(24- 48hrs)pl:l5.86.47.0pH4. 5-5.2pH5.8QHS QHSN6DCRLitvay1601-L-1WSWhiteB5DCRQHS L7(3-)QHSMg2+Fe2+Mn-2+NH4NO3KN03 ,KI,Ca-2+NH4N03KNOsMg2+Ca2+QHS TLCQHSQHS QHSQHSHPlQHS2 QHSQHS3QHS4QHSQHSQIS51601-L-115834-L-1

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(Allium sativumL)(Cynodon dactylon) (Metallothioneins LikeMTs Like)Y-(Glu-Cys) n-Gly(PhytochelatinsPCs)MT LikePCs RACE(MT-Like)cDNA(GenBank Accession No.AY050510)PCRSoutheLrn BlotMT Like cDNAMT Like cDNA73121 6.4%89%N-c-3Cys-Xaa-CysType-1Cys-Xaa-CysMT LikeMT LikeMT LikeCu2+Cd2+MT LikeMT Like Y-AlaPCsGSHy(PCsphytochelatin synthasePCS)PCs345bpcDNAcDNAcDNA(GenBank Accession No.AF384110)(MT LikePCS) M379/8

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;19;30 25pH5.35.8O,l%-0.30% Cu2+Mg2+Zn2+K+NH;50gLIAAKT Cu2+

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, EDTA 1 391 KUg - 1 ,,1 2 5 mmolL - 1Ca2 + Mg2 + Mn2 + ,1 2 mmolL - 1Zn2 + Co2 + ,5 mmolL - 1Zn2 + Co2 + ,Cu2 + 1 2 5 mmolL - 1 , EDTA , EDTA

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Cu2+,Cu2+:>>>>Cu-SO4.5H2O(102μg/LCu2+):,;;,,,,Cu2+;,

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Cu2+(0.01,0.1,1,10,50,100,200mg/L)(Chlorococcumsp.).,Cu2+.BG11,0.011mg/LCu2+,,,;(10200mg/LCu2+),,,1.Cu2+(0.01,0.1mg

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Cd2 + Cu2 + BF5(TetrahymenathermophilaBF5) , :Cd2 + (0 0 .4mgL-1)Cu2 + (0 10mgL-1) ,Cd2 + (0 .8 3.2mgL-1)Cu2 + (2 0 2 0 0mgL-1) ;Cd2 + Cu2 + IC50 2 .0 4mg/L15 5 .5mg/L ;

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Cu2 + ,Cu2 + Kinneret ,Cu2 + Kinneret , ,Cu2 + , ,Cu2 + Kinneret , 2 0

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, , , 5d ,Cu2 + , 54KDa ;Zn2 + , 94KDa ,67KDa 40KDa

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0100130180240.316mgLCu2Cu2020402970316mgLCu2

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96h2hCu2EC500105mgLCu2EC500204mgL

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A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P-petE-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P-petE-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P-petE-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P-petE-vipp1 cells grown in medium with 0.025 mu M Cu2+ showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803.

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Total alkaline phosphatase activity (APA) and soluble reactive phosphorus (SRP) concentrations were measured in municipal wastewater, and a shallow Chinese freshwater lake receiving it. Activities of Dissolved alkaline phosphatase ( ADAP) in overlying and interstitial water were also analyzed monthly at three sites for several years. The lake was enriched with SRP and alkaline phosphatase by discharge of the wastewater, indicating that the inclusion of APA for estimating water pollution was reasonable. Annual data showed that APA in coarser fraction was significantly higher at the site receiving more wastewaters, both in surface and overlying water, suggesting that resuspension of enzyme most likely occurred in the basin heavily discharged. ADAP was an order of magnitude higher in the wastewater than those in lake waters, and was generally higher in interstitial water, a feature more striking at the site receiving more discharges. Besides, it was irrespectively inhibited by Na2WO4, L-cysteine and EDTA-Na, but stimulated by Cu2+, Zn2+, CTAB and Triton X-100 in interstitial, overlying and surface waters. This similarity of responding patterns to the stressors indicated an analogy between dissolved alkaline phosphatase in water column and that in interstitial water, supporting the hypothesis that the polluted sediments act as source of dissolved alkaline phosphatase in eutrophic lakes.

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The pressure dependence of the photoluminescence from ZnS : Mn2+, ZnS : Cu2+, and ZnS : Eu2+ nanoparticles were investigated under hydrostatic pressure up to 6 GPa at room temperature. Both the orange emission from the T-4(1) - (6)A(1) transition of Mn2+ ions and the blue emission from the DA pair transition in the ZnS host were observed in the Mn-doped samples. The measured pressure coefficients are -34.3(8) meV/GPa for the Mn-related emission and -3(3) meV/GPa for the DA band, respectively. The emission corresponding to the 4f(6)5d(1) - 4f(7) transition of Eu2+ ions and the emission related to the transition from the conduction band of ZnS to the t(2) level of Cu2+ ions were observed in the Eu- and Cu-doped samples, respectively. The pressure coefficient of the Eu-related emission was found to be 24.1(5) meV/GPa, while that of the Cu-related emission is 63.2(9) meV/GPa. The size dependence of the pressure coefficients for the Mn-related emission was also investigated. The Mn emission shifts to lower energies with increasing pressure and the shift rate (the absolute value of the pressure coefficient) is larger in the ZnS : Mn2+ nanoparticles than in bulk. Moreover, the absolute pressure coefficient increases with the decrease of the particle size. The pressure coefficients calculated based on the crystal field theory are in agreement with the experimental results. (C) 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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The room-temperature photoluminescence (PL) of copper doped zinc sulfide (ZnS:Cu) nanoparticles were investigated. These ZnS:Cu nanoparticles were synthesized by a facile wet chemical method, with the copper concentration varying from 0 to 2 mol%. By Gaussian fitting, the PL spectrum of the undoped ZnS nanoparticles was deconvoluted into two blue luminescence peaks (centered at 411 nm and 455 nm, respectively), which both can be attributed to the recombination of the defect sates of ZnS. But for the doped samples, a third peak at about 500 nm was also identified. This green luminescence originates from the recombination between the shallow donor level (sulfur vacancy) and the t(2) level of Cu2+. With the increase of the CU2+ concentration, the green emission peak is systematically shifted to longer wavelength. In addition, it was found that the overall photoluminescence intensity is decreased at the Cu2+ concentration of 2%. The concentration quenching of the luminescence may be caused by the formation of CuS compound. (c) 2005 Elsevier B.V. All rights reserved.