长薄鳅年龄与生长的研究

在长江上游收集长薄鳅标本,对其年龄与生长进行了研究。以鳞片、耳石、脊椎骨和胸鳍鳍条为年龄鉴定材料。透射光下,鳞片的环沟和辐射沟呈现透明的凹沟。长薄鳅的鳞径与体长呈显著的正相关,回归方程为L=0.4326+82.5738R(n=37,r=0.9899);体长与体重的关系方程为W=4.736×10-3L3.3045(n=37,R2=0.9971);生长方程为L=-1.3189+5.2132t(r=0.9892)。

亚东鲑的年龄与生长的研究

首次报道了亚东鲑在青藏高原河流中的生长特点。对1999—2002年间在亚东河共采集的462尾亚东鲑(体长范围为93—364mm)进行了年龄与生长的研究。通过鳞片对其中302尾进行年龄鉴定并建立Von Bertalanffy生长方程:雌性(♀)Lt=709.44[1-e-0.1656(t-0.0845)](R2=0.9993,n=132)和Wt=6017.46[1-e-0.1656(t-0.0845)]2.9724(R2=0.9845,n=132);雄性(♂)Lt=797.82[1-e-0.1428(t-0

鱼肉结合态MCLR的亚慢性毒性研究

为了揭示含有微囊藻毒素水产品的食用安全性,以雄性BALB/C小鼠为模式生物,通过为期13周的灌胃染毒实验,研究了鱼肉结合态MCLR对小鼠的亚慢性毒性作用,并与水溶态MCLR的毒性进行了对比.结果表明,68.75μg/kg(以单位体重计)剂量水溶态MCLR可引起小鼠肝脏显著系数增加(p<0.05),ALT、AST活性增强(p<0.01),引起肝细胞出现有空泡状病变;而肾脏酶学指标BUN、Cr及肾脏组织病理学观察均未发现明显异常;与之相比,同剂量鱼肉结合态MCLR对小鼠各项指标的影响并不明显,仅在实验第1周出

香溪河库湾春季水华期间可溶性碳动力学研究

2005年2月23日—4月28日,对三峡水库香溪河库湾内一样点进行每天采样,监测水体中叶绿素a与可溶性碳(DOC)浓度的变化,研究在春季水华暴发期间DOC的动力学特性。监测结果表明,随着时间的推移,叶绿素a有逐渐升高的趋势。其间共暴发了两次水华,其中第一次历时较短,第二次历时较长。DOC的变化趋势与叶绿素a基本吻合。整个暴发过程中的叶绿素a与DOC的回归分析表明,二者具有较好的相关性(R2=0.62),但第一次暴发过程中的相关性很高(R2=0.72),而第二次暴发过程中的相关性较低(R2=0.30)。根据

保定市工业废水对白洋淀微型生物群落的生态毒性

以白洋淀PFU(PolyurethaneFoamUnit)微型生物群落为种源(epicenter),评价上游工业废水的生态毒性.结果表明,在反映原生动物群集过程的3个参数Seq、G和T90%中,Seq与工业废水体积分数(φ)呈负相关.根据其回归方程Seq=42.22857-0.33374φ(R2=0.9042,P<0.01)推算出工业废水的效应浓度EC5、EC20和EC50,分别为6.3%、25.3%和65.2%.以此确定上游工业废水对白洋淀微型生物群落的无效应浓度为6.3%,最大允许浓度(MATC)为2

日本三陆沿岸白胸拟鼠海豚体内铁的蓄积(英文)

根据22头日本三陆沿岸白胸拟鼠海豚的检测结果,研究了必需元素铁在13种组织和器官中的蓄积特征。肺、肝脏和脾脏中铁的浓度显著高于其它脏器,铁在皮肤和鲸脂中的浓度却非常低。研究发现,鲸类肝脏中铁的平均浓度与其最大潜水持续时间有着极其显著的正相关(y=285.17x0.5267;r2=0.92;y:a肝脏中铁的平均浓度,单位为μg·g-1干重;x:最大潜水持续时间,单位为min)。利用该关系式,首次推测白胸拟鼠海豚的最大潜水持续时间为11.85min。

Effect of water temperature on the growth performance and digestive enzyme activities of Chinese longsnout catfish (Leiocassis longirostris Gunther)

The present study was carried out to investigate the influence of water temperature on the growth performance and digestive enzyme (pepsin, trypsin and lipase) activities of Chinese longsnout catfish. Triplicate groups of Chinese longsnout catfish (35.6 +/- 0.48 g, mean +/- SE) were reared at different water temperatures (20, 24, 28 and 32 degrees C). The feeding rate (FR), specific growth rate (SGR) and feed efficiency ratio (FER) were significantly affected by water temperatures and regression relationships between water temperature and FI, SGR as well as FER were expressed as FR=-0.016T2+0.91T-10.88 (n=12, R2=0.8752), SGR=-0.026T2+1.39T-17.29 (n=12, R2=0.7599) and FER=-0.013T2+0.70T-8.43 (n=12, R2=0.7272). Based on these, the optimum temperatures for FR, SGR and FER were 27.66, 26.69 and 26.44 degrees C respectively. The specific activities of digestive enzymes at 24 or 28 degrees C were significantly higher than that at 20 or 32 degrees C. In addition, there was a significant linear regression between FR or SGR and specific activities of pepsin and lipase, which indicated that pepsin and lipase played important roles in regulating growth through nutrient digestion in Chinese longsnout catfish.

The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss

Interferons (IFNs), consisting of three major subfamilies, type I, type II (gamma) and type III (lambda) IFN, activate vertebrate antiviral defences once bound to their receptors. The three IFN subfamilies bind to different receptors, IFNAR1 and IFNAR2 for type I IFNs, IFN gamma R1 and IFN gamma R2 for type II IFN, and IL-28R1 and IL-10R2 for type III IFNs. In fish, although many types I and II IFN genes have been cloned, little is known about their receptors. In this report, two putative IFN-gamma receptor chains were identified and sequenced in rainbow trout (Oncorhynchus mykiss), and found to have many common characteristics with mammalian type II IFN receptor family members. The presented gene synteny analysis, phylogenetic tree analysis and ligand binding analysis all suggest that these molecules are the authentic IFN gamma Rs in fish. They are widely expressed in tissues, with IFN gamma R1 typically more highly expressed than IFN gamma R2. Using the trout RTG-2 cell line it was possible to show that the individual chains could be differentially modulated, with rIFN-gamma and rIL-1 beta down regulating IFN gamma R1 expression but up regulating IFN gamma R2 expression. Overexpression of the two receptor chains in RTG-2 cells revealed that the level of IFN gamma R2 transcript was crucial for responsiveness to rIFN-gamma, in terms of inducing gamma IP expression. Transfection experiments showed that the two putative receptors specifically bound to rIFN-gamma. These findings are discussed in the context of how the IFN gamma R may bind IFN-gamma in fish and the importance of the individual receptor chains to signal transduction. (c) 2009 Elsevier Ltd. All rights reserved.

Plasma biochemical responses of the planktivorous filter-feeding silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) to prolonged toxic cyanobacterial blooms in natural waters

The planktivorous filter-feeding silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) are the attractive candidates for bio-control of plankton communities to eliminate odorous populations of cyanobacteria. However, few studies focused on the health of such fishes in natural water body with vigorous toxic blooms. Blood parameters are useful and sensitive for diagnosis of diseases and monitoring of the physiological status of fish exposed to toxicants. To evaluate the impact of toxic cyanobacterial blooms on the planktivorous fish, 12 serum chemistry variables were investigated in silver carp and bighead carp for 9 months, in a large net cage in Meiliang Bay, a hypereutrophic region of Lake Taihu. The results confirmed adverse effects of cyanobacterial blooms on two phytoplanktivorous fish, which mainly characterized with potential toxicogenomic effects and metabolism disorders in liver, and kidney dysfunction. In addition, cholestasis was intensively implied by distinct elevation of all four related biomarkers (ALP, GGT, DBIL, TBIL) in bighead carp. The combination of LDH, AST activities and DBIL, URIC contents for silver carp, and the combination of ALT. ALP activities and TBIL, DBIL. URIC concentrations for bighead carps were found to most strongly indicate toxic effects from cyanobacterial blooms in such fishes by a multivariate discriminant analysis. (C) 2009 Elsevier B.V. All rights reserved.

Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China

Some species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10-24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), gamma-glutamyl transpepticlase (gamma-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 24: 10-18, 2009.

Plasma biochemical responses of the omnivorous crucian carp (Carassius auratus) to crude cyanobacterial extracts

Healthy crucian carp (Carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 mu g MC-LR equiv kg(-1) BW. High mortality (100%) was observed within 60 h post injection in the high-dose group. In the treated fish, activities of four plasma enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), all showed substantial increases, with both dose and time-dependent effects. These increases of enzyme activity indicate severe impairment occurred in the liver of crucian carp over time. Plasma concentrations of energy-related biomolecules including glucose (GLU), cholesterol (CHO), triglyceride (TG), and total protein (TP) showed marked changes in the high-dose group, possibly a nutritional imbalance correlated with the liver injury caused by intraperitoneal exposure to crude cyanobacterial extracts.

Sequential ultrastructural and biochemical changes induced in vivo by the hepatotoxic microcystins in liver of the phytoplanktivorous silver carp Hypophthalmichthys molitrix

Up to now, in vivo studies on the toxic effects of microcystins (MCs) on the ultrastructures of fish liver have been very limited. The phytoplanktivorous silver carp was injected i.p. with extracted hepatotoxic microcystins (mainly MC-RR and -LR) at a dose of 1000 mu g MC-LReq. kg(-1) body weight, showing a time-dependent ultrastructural change in liver as well as significant increases in enzyme activity of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH). We observed for the first time the occurrence of a large amount of activated secondary lysosomes, which might be an adaptive mechanism to eliminate or lessen cell damage caused by MCs through lysosome activation. Quantitative and qualitative determinations of MCs in the liver were conducted by HPLC and LC-MS2, respectively. MCs concentration in the liver reached the maximum (114.20 mu g g(-1) dry weight) after 3 h post-injection, and then rapidly dropped to 7.57 mu g g(-1) dry weight at 48 h, indicating a deputation of 99% accumulated MC-LReq. On the other hand, a decrease trend in glutathione (GSH) concentration was observed in the liver of silver carp while the activity of glutathione S-transferase (GST) increased significantly after injection. The high tolerance of silver carp to MCs might be due to the high basic GSH level in their liver, and/or an increased GSH synthesis. (C) 2007 Elsevier Inc. All rights reserved.

黄土区人工与天然植被对凋落物量和土壤有机碳变化的影响

在黄土丘陵区选择从耕地、草地、灌木林到乔木林样地,不同样地内设立1m×1m(乔木10m×10m)的样方,分析样方内凋落物积累量、碳氮含量、土壤有机碳(SOC)和可溶性碳(DOC)含量变化。结果表明:天然草地、灌木林、乔木林凋落物积累量依次为5.3,12.1和32.4t.hm-2;但人工灌木林和乔木林的凋落物积累量分别为6.7和11.4t.hm-2,分别是天然灌木林和乔木林的1/2和1/3。随着植被的恢复,天然植被凋落物的C/N高于人工植被(刺槐林除外)。与耕地SOC(4.67g·kg-1)相比,天然灌木林地SOC提高5.9倍,人工灌木林地提高1.8倍;天然乔木林地提高8.0倍,而人工乔木林地仅提高4.0倍。凋落物积累量与0～20cm土层土壤有机碳存在显著线性相关关系(R2>0.83),但20cm以下线性相关关系不显著。凋落物积累量与0～10cm土壤可溶性碳含量存在显著线性相关关系(R2>0.893),与10～60cm土层线性相关关系不显著,与80～100cm土壤可溶性碳存在显著线性负相关关系。

黄土高原长期施用磷肥效应研究

以黄土高原长期定位试验为基础,研究施一定量氮肥(90 kg/hm2)的前提下,长期施用磷肥对黄土高原旱作冬小麦的肥料效应。结果表明,长期施用磷肥的农田施磷仍能显著提高小麦的产量,增产量达1393.75~2121.00kg/hm2,增产率为48.41%~73.67%,本试验中施磷39.6 kg/hm2时小麦产量达最大值5000 kg/hm2,这与小麦成穗数最大时的施肥量结果一致;产量与施磷量关系用回归方程Y=-0.8667X2+82.641X+3008.4(R2=0.92)拟合效果良好;施磷主要是通过影响小麦成穗数来影响小麦产量,而对穗粒数和千粒重的促进作用不明显;施磷还可促进小麦对氮磷钾养分的吸收利用,提高肥料的肥效和利用率;施磷过少不能满足作物需求,小麦产量较低,施磷过多小麦产量不会随施肥量的增加继续提高,反而有一定幅度的下降。

氮肥缺失对旱地土壤细菌群落多样性的影响

本文旨在研究氮肥缺失对旱地土壤细菌群落多样性的影响。采用直接提取土壤微生物总DNA的方法,对不施肥(CK)、适量施肥(F1)、和缺氮施肥(F2)3种不同施肥水平土样DNA进行提取,扩增细菌16S rDNA基因片段,建立克隆文库。用限制性内切酶HhaI和RsaI进行PCR-RFLP分析,分别得到146、187、11个酶切类型。采用α多样性的测度对试验结果进行分析统计结果表明,不同处理间土壤细菌的多样性(H′、Ds和Dg)和物种丰富度(dMa、R2和E)均为F1>CK>F2;λ、dMa、E和H′指数在不同施肥处理间的变异系数达到56.96%~163.1%,尤其Simpson指数λ是非常敏感的指标,处理间的差异最大,表明氮肥缺失严重影响土壤细菌群落多样性,合理施肥有利于土壤细菌的多样性。