Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation

Objective The purpose of this study is to investigate the effect of subdinical-dose C-12(6+)-beam irradiation on cell cycle and cell apoptosis in hepatocarcinoma cells. Materials and methods The HepG(2) cells were exposed to 0-2.0 Gy of either the C-12(6+) beam or a gamma-ray. Cell survival was detected by clonogenic assay. Cell cycle was determined by flow-cytometry analysis. The apoptosis was monitored by fluorescence microscope with DAPI staining. p53 and p21 expression were detected by Western blot. Results The G(0)/G(1) cells in the irradiated groups were significantly more than those in the control (P<0.05). The C-12(6+)-ion irradiation had a greater effect on the cell cycle of HepG(2) cells (including promoting G(1)-phase and G(2)-phase arrest) than gamma-ray irradiation. The apoptotic cells induced by C-12(6+) beam were significantly more numerous than those induced by gamma-ray (P<0.05). The carbon ions had a stronger effect on p53 and p21 expression than the gamma-ray irradiation. The survival fractions for cells irradiated by C-12(6+) beam were significantly smaller than those irradiated by gamma-ray (P<0.05).

Pb isotope shifts in the relativistic mean field theory

The ground state properties of the Pb isotopic are studied by using the axially deformed relativistic mean field (RMF) calculation with the parameter set TM1. The pairing correlation is treated by the BCS method and the isospin dependent pairing force is used. The 'blocking' method is used to deal with unpaired nucleons. The theoretical results show that the relativistic mean field theory with non-linear self-interactions of mesons provides a good description of the binding energy and neutron separation energy. The present paper focus on the physical mechanism of the Pb isotope shifts.

大兴安岭呼中区紫貂生境格局变化及采伐的影响

本文根据紫貂(Martes zibellina)行为生态学特征,利用景观生态学的原理和技术,将大兴安岭呼中区紫貂生境划分成最适、一般适宜和非适宜三种类型。以其紧邻的呼中自然保护区核心区1989年的生境格局代表呼中区1970年开发前的原始生境,揭示原始生境、1989年、2000年3个时期紫貂生境格局及变化。结果表明,1970-2000年紫貂生境格局持续恶化,已极不利于紫貂的生存。适宜生境向非适宜生境呈单一方向转变,且适宜生境由沟谷两侧向远离河谷的森林腹地大幅度萎缩。生境格局变化与经营时间长短关系密切,原始状态是以适宜生境为基质的均质状态,开发至一定时期,生境格局的异质化程度增大,继续开发则异质化程度又减小,形成以非适宜生境为基质的相对均质状态。大于巢区最小面积(4km2)的生境斑块的面积百分比、斑块密度明显下降,斑块间距离显著增大。进而对导致生境格局变化的要素进行分析后发现,持续采伐是紫貂生境格局变化的主要驱动因子,采伐改变了植被条件,而植被条件的非适宜性造成了紫貂非适宜生境。1989-2000年的采伐格局也并不合理,各年伐区在研究区内均匀分布,采伐设计时没有考虑动物生境保护。但可以推断,1999年后开始逐步实施的“天然林保护工程”有利于紫貂生境的恢复,本研究强调森林采伐经营的空间格局与动物生境保护之间的关系,旨在促进决策部门将生物保护作为采伐经营政策制定的重要决策因子。

土地资源动态监测信息系统——以延安/安塞七乡镇为例

土地资源动态监测信息系统是在计算机软硬件支持下 ,对土地资源采集、输入、编辑、存储和综合分析应用的技术系统 ,它是土地资源动态监测、土地评价、土地利用规划和管理的有力工具。本文以延安 /安塞七乡镇为例 ,启用 ARC/INFO和 Arcview软件 ,建立土地资源动态监测系统 ,并对监测结果初步分析 ,为区域土地资源管理和合理利用提供服务

容器苗质量评定指标的研究

In this paper,the assessing indices of container seedlings quality were synthesized in accordance with growth and physiological factors. The results showed that the seedling quality might be determined by the dormancy state of terminal bud,nutrient condition of plant,and the state of root system. The critical quality indices should include stem diameter at soil surface,dry weight of terminal bud,and soluble sugar content. Moreover,some indices such as root system vitality and dry weight of plant were also important.

箱式气体交换观测系统及其在植物生态系统气体交换研究中的应用

基于箱式法构建的箱式气体交换观测系统是植物生态系统碳水循环研究的常用方法,其在土壤和植物组织尺度的气体交换研究中的作用尤为重要.本文根据箱式气体交换观测系统的气路结构,对闭路、半闭路、开路和半开路系统的原理和计算方法进行了详细总结;汇总了目前常用的商业箱式气体交换观测系统,并回顾了自制系统的研制和应用历程;最后简要探讨了箱式气体交换观测系统存在的问题及其改进方式.

夏朝前夕洪水发生的可能性及大禹治水真相

文章首先根据古文献学、考古学以及天文学等多学科交叉研究的成果,分析了史前洪水可能发生的时代,然后利用高分辨率的气候代用指标重建了“大禹治水”传说时期的气候背景,推测传说中尧舜禹时期的大洪水发生于4200~4000aB·P.左右,即一次对世界许多地区早期文明发展进程产生重大影响的全球性气候异常时期;根据气候突变与东亚季风降水之间的关系论证了洪水发生的可能性,探讨了大禹治水的历史真相;认为夏朝建立前夕的史前大洪水是真实发生过的,而大禹之所以能够治水成功可能主要得益于4000aB·P.以后的气候好转而并非人力之