48 resultados para 54301-008
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The neutron (PF2)-P-3 pairing gap in pure neutron matter, neutron (PF2)-P-3 gap and neutron-proton (SD1)-S-3 gap in symmetric nuclear matter have been studied by using the Brueckner-Hartree-Fock(BHF) approach and the BCS theory. We have concentrated on investigating and discussing the three-body force effect on the nucleon superfluidity. The calculated results indicate that the three-body force enhances remaxkably the (PF2)-P-3 superfluidity in neutron matter. It also enhances the (PF2)-P-3 superfluidity in symmetric nuclear matter and its effect increases monotonically as the Fermi-momentum k(F) increases, whereas the three-body force is shown to influence only weakly the neutron-proton (SD1)-S-3 gap in symmetric nuclear matter.
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Objective To investigate whether the irradiation with C-beam could enhance adenovirus-mediated transfer and expression of p53 in human hepatocellular carcinoma. Materials and methods HepG2 cells were exposed to C-beam or gamma-ray and then infected with replicationdeficient adenovirus recombinant vectors containing human wild-type p53 or green fluorescent protein, respectively. The transfer efficiency and expression level of the exogenous gene were detected by flow cytometric analysis. Cell survival fraction was detected by clonogenic assay. Results The transfer frequency in C-beam or gamma-irradiated groups increased by 50-83% and 5.7-38.0% compared with the control, respectively (P < 0.05). Compared with C-beam alone, p53 alone, and gamma-ray with p53, the percentages of p53 positive cells for 1 Gy C-beam with p53 increased by 56.0-72.0%, 63.5-82.0%, and 31.3-72.5% on first and third day after the treatments, respectively (P < 0.05). The survival fractions for the 2Gy C-bearn and AdCMV-p53 infection groups decreased to similar to 2%. Conclusion C-beam irradiation could significantly promote AdCMV-green fluorescent protein transfer and expression of p53.
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We investigate the effect of the calar-isovector delta-meson field on the equation of state (EOS) and composition of hyperonic neutron star matter, and the properties of hyperonic neutron stars within the frame work of the relativistic mean field theory. The influence of the delta-field turns out to be quite different and generally weaker for hyperonic neutron star matter as compared to that for npe mu neutron star matter. We find that inclusion of the delta-field enhances the strangeness content slightly and consequently moderately softens the EOS of neutron star matter in its hyperonic phase. As for the composition of hyperonic star matter, the effect of the delta-field is shown to shift the onset of the negatively-charged (positively-charged) hyperons to slightly lower (higher) densities and to enhance (reduce) their abundances. The influence of the delta-field on the maximum mass of hyperonic neutron stars is found to be fairly weak, where as inclusion of the delta-field turns out to enhance sizably both the radii and the moments of inertia of neutron stars with given masses. It is also shown that the effects of the delta-field on the properties of hyperonic neutron stars remain similar in the case of switching off the Sigma hyperons.
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对潮棕壤水稻田、玉米地、撂荒地和人工林地 4 种土地利用方式经过 14 年后在 0~150 cm 土体 10个土层中土壤有机碳含量的剖面分布、C/N 及有机碳储量进行比较研究。结果表明,不同利用方式下土壤有机碳含量产生明显的剖面分布差异。林地各土层有机碳含量较其它几种利用方式高,表明不同利用方式对土壤碳产生较大影响;土壤有机碳与全氮极显著相关,但自然生态系统中碳与氮的相关性略高于农田生态系统(林地R =0.990,撂荒地 R =0.990,稻田 R =0.976,玉米地 R =0.980,P < 0.001,n =30);剖面中 C/N 随深度而 2 2 2 2下降,林地 C/N 较高,稻田 C/N 较低,玉米地与撂荒地相应土层中 C/N 相近;在 100 cm 深度内,林地土壤分别比稻田、玉米地、撂荒地每年多截获 4.25、2.87 和 4.48 t·ha-1有机碳,年增幅分别为 6.15%、3.26%和 5.09%;林地有机碳储量显著高于稻田、玉米地和撂荒地(P 值分别达到 0.001、0.008 和 0.008),其它 3 种利用方式间差异不显著。据此认为林地在增加碳储量及改善环境方面具有很大的潜力。
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目的查明内蒙古陈巴尔虎旗饮茶型氟、铝联合中毒原因。方法在陈巴尔虎旗3个牧区嘎查随机抽取93名20~50岁牧民进行发、血清、尿含氟、铝水平测定,并拍摄前臂、小腿、骨盆正位及腰椎正侧位X线片,同时检测饮水、牛奶、砖茶等氟和铝水平,以扎兰屯市地方病防治研究所的34名23~50岁职工为对照组。结果陈巴尔虎旗牧民发、血清、尿及饮用的奶茶含铝量分别是(22.34±8.06)mg/kg、(1.13±0.48)mg/L、(3.78±1.62)mg/L、(6.02±1.77)mg/L,含氟量分别是(1.21±0.63)mg/kg、(0.092±0.042)mg/L、(2.08±1.16)mg/L、(3.00±1.11)mg/L,饮水、牛奶含铝量分别是(39±16)μg/L、(0.553±0.179)mg/L,含氟量分别是(0.50±0.39)、(0.063±0.032)mg/L;对照组发、血清、尿铝分别是(5.80±2.14)mg/kg,(0.203±0.101)mg/L、(2.19±1.34)mg/L,含氟量分别是(1.17±0.68)mg/kg、(0.033±0.008)mg/L、(0.57±0.32)mg/L,牧民发、血清、尿铝及血清、尿氟均高于对照组(P<0.001)。牧民X线改变硬化型22.2%(20/88),疏松型37.5%(33/88),退行性变86.4%(79/88),对照组退行性变13.3%(4/30),其余改变未检出。结论陈巴尔虎旗牧区饮茶型氟中毒实际为饮茶型氟、铝联合中毒。
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Reducing uncertainties in the estimation of land surface evapotranspiration (ET) from remote-sensing data is essential to better understand earth-atmosphere interactions. This paper demonstrates the applicability of temperature-vegetation index triangle (T-s-VI) method in estimating regional ET and evaporative fraction (EF, defined as the ratio of latent heat flux to surface available energy) from MODIS/Terra and MODIS/Aqua products in a semiarid region. We have compared the satellite-based estimates of ET and EF with eddy covariance measurements made over 4 years at two semiarid grassland sites: Audubon Ranch (AR) and Kendall Grassland (KG). The lack of closure in the eddy covariance measured surface energy components is shown to be more serious at MODIS/Aqua overpass time than that at MODIS/Terra overpass time for both AR and KG sites. The T-s-VI-derived EF could reproduce in situ EF reasonably well with BIAS and root-mean-square difference (RMSD) of less than 0.07 and 0.13, respectively. Surface net radiation has been shown to be systematically overestimated by as large as about 60 W/m(2). Satisfactory validation results of the T-s-VI-derived sensible and latent heat fluxes have been obtained with RMSD within 54 W/m(2). The simplicity and yet easy use of the T-s-VI triangle method show a great potential in estimating regional ET with highly acceptable accuracy that is of critical significance in better understanding water and energy budgets on the Earth. Nevertheless, more validation work should be carried out over various climatic regions and under other different land use/land cover conditions in the future.
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The rapid scan spectrometer was used to determine the heterogeneous electron transfer rate parameters for the oxidation of Biliverdin in DMF by single potential step thin layer spectroelectrochemical techniques and yielded an average formal heterogeneous electron transfer rate constant K(s, h)0' = 2.45 (+/-0.12) x 10(-4) cm s-1, electrochemical transfer coefficient alpha = 0.694+/-0.008. The oxidation process of Biliverdin was also studied and the formal potential E0 = 0.637 V (vs. Ag/AgCl) was obtained.
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Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30A degrees C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237 +/- 0.026 g g(-1) cell dry weight and 0.272 +/- 0.041 g L-1 when glucose was used as the carbon source, whereas the lipid content reached 0.287 +/- 0.018 g g(-1) cell dry weight and 0.288 +/- 0.008 g L-1 when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L-1 gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.
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Genetic variation of four populations of Sargassum thunbergii (Mert.) O. Kuntze and one outgroup of S. fusiforme (Harv.) Setchell from Shandong peninsula of China was studied with random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. A total of 28 RAPD primers and 19 ISSR primers were amplified, showing 174 loci and 125 loci, respectively. Calculation of genetic diversity with different indicators (P%, percentage of polymorphic loci; H, the expected heterozygosity; I, Shannon's information index) revealed low or moderate levels of genetic variations within each S. thunbergii population. High genetic differentiations were determined with pairwise Nei's unbiased genetic distance (D) and fixation index (F-ST) between the populations. The Mantel test showed that two types of matrices of D and FST were highly correlated, whether from RAPD or ISSR data, r=0.9310 (P = 0.008) and 0.9313 (P=0.009) respectively. Analysis of molecular variance (AMOVA) was used to apportion the variations between and within the S. thunbergii populations. It indicated that the variations among populations were higher than those within populations, being 57.57% versus 42.43% by RAPD and 59.52% versus 40.08% by ISSR, respectively. Furthermore, the Mantel test suggested that the genetic differentiations between the four populations were related to the geographical distances (r > 0.5), i.e., they conformed to the IBD (isolation by distance) model, as expected from UPGMA (unweighted pair group method with arithmetic averages) cluster analysis. As a whole, the high genetic structuring between the four S. thunbergii populations along distant locations was clearly indicated in the RAPD and ISSR analyses (r > 0.8) in our study.
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The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop.
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本论文报道从海洋中分离到的一株聚磷菌的分离、鉴定、在系统发育中的地位、除磷特性、菌体内多磷酸盐颗粒的研究、D-海因酶和核苷二磷酸激酶基因的克隆及序列分析,为海水系统的生物除磷提供部分基础资料。 从黄海海域分离到聚磷菌Halomonas sp. YSR-3,菌体呈杆状,大小为3.5 μm×1 μm,革兰氏阴性,好氧生长,能运动。透射电镜观察发现,菌体内有致密颗粒。经DAPI染色确定该致密颗粒是多磷酸盐,亦可称为异染粒、迂回体。16S rDNA鉴定结果表明,YSR-3与Halomonas属中的marine bacterium B5-7有较高的同源性,相似值99%。YSR-3的生理生化特性:对氯霉素和卡那霉素敏感;淀粉水解呈阳性;反硝化和几丁质降解呈阴性;能将葡萄糖作为唯一碳源和能源。 对YSR-3的培养条件进行优化。以海水2216培养基、24 ℃、180 rpm、pH 6.5的条件培养,更利于菌体生长和菌体内多磷酸盐的形成。 对YSR-3的除磷特性进行研究。无磷培养时,菌体不能生长;用磷酸钾盐作为磷源时,菌体生长较好,形成多磷酸盐的菌体比例较高;较适合YSR-3菌体生长和多磷酸盐形成的磷源是KH2PO4,较适磷浓度为1.5 mmol/L。pH的变化影响菌株的生长、多磷酸盐形成和除磷效果。pH值为5时,菌体的数量几乎不增加,体内多磷酸盐和培养基中磷含量变化不大;pH值为6、7和8时,菌体生长良好,95%以上的菌体内形成多磷酸盐,培养基中磷含量明显下降。YSR-3在不同培养基中除磷量和除磷率不同。在高磷培养基中除磷量为0.7 mmol/L(磷含量由1.84 mmol/L降到1.14 mmol/L),除磷率为37.5%;在低磷培养基中除磷量为0.02 mmol/L(磷含量由0.028 mmol/L降到0.008 mmol/L),除磷率为72.2%。 以海洋聚磷菌Halomonas sp. YSR-3的总DNA为模板,用PCR法扩增D-海因酶基因和核苷二磷酸激酶基因,将扩增片段克隆到pGM-T载体,转化E.coli TOP10菌株,经蓝白斑筛选、菌落PCR得到阳性克隆,测序后对序列进行Blast比对分析。得到的D-海因酶基因序列长度为1510 bp,与Pseudomonas entomophila L48的海因酶基因序列的相似性为77%。翻译后的序列与Pseudomonas fluorescens Pf-5,Marinomonas sp. MED121,Burkholderia vietnamiensis G4的海因酶蛋白序列相似性分别为75%,73%,70%。得到的核苷二磷酸激酶基因序列长度为420bp,翻译后的序列与Loktanella vestfoldensis SKA53,Jannaschia sp. CCS1,Roseobacter sp. CCS2的核苷二磷酸激酶蛋白序列相似性分别为89%,86%,85%。 聚磷菌能将外界环境中的磷吸收到体内,并以多磷酸盐的形式储存。多磷酸盐对于细胞的生存和生长有很重要的作用,但目前对于多磷酸盐的形成过程以及过程调控还不是很清楚。在今后可以通过构建高效表达的重组菌,提高与除磷相关的酶的纯度及活性。同时可以将相关酶的基因进行突变,对基因表达的调控以及酶的代谢以及功能结构等多方面进行基础研究,使聚磷菌在生物除磷中得到广泛应用。
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本文以南黄海1997~2006年10年表层海水和沉积物中重金属为主要研究对象,同时结合对生态环境信息的综合分析,系统探讨了海水和沉积物中重金属的生物地球化学特征、影响控制因素、演变趋势,并对海域生态风险进行了评估,获得了以下一系列新的结果和认识: 1.系统获得了南黄海海水和沉积物中重金属的地球化学分布模式,揭示了影响和控制其生物地球化学特征的因素 南黄海表层海水中重金属As、Cd、Cu、Hg、Pb、Zn的平均浓度分别为2.33、0.078、1.41、0.0036、0.37、6.21 μg/L,低于其它中国近海海水,而高于水交换较好的深海;表层海水重金属的分布模式(除Pb外)表现为在离岸较远的南黄海中部地区其含量较低,而近岸海区则普遍含量较高,区域分布呈现“高Cd-Cu-Hg-Zn区”,“高Pb-Cu-Zn区”以及“高Pb区”三个地球化学分区。 南黄海表层沉积物中重金属比邻近海域沉积物中的浓度低,南黄海重金属主要受沉积物粒度控制,即在细粒度高的南黄海中部区域重金属(除As外)的含量较高,粗粒度的近岸区则较低,区域分布呈现“高Cd-Cu-Pb-Zn区”,“高Hg低As-Cu-Zn区”以及“高As低Cd-Hg-Zn区”三个地球化学分区。 人类活动已经显著影响了南黄海海水中重金属的含量水平,重金属分布是径流、大气沉降、pH、盐度和重金属自身性质等各种影响因子耦合的结果。沉积物重金属的富集因子Pb>As>Hg>Cd>Zn>Cr>Cu,其中Pb和As主要来自人为污染排放,污染状况相对较重,Cr和Cu几乎没有受到人为污染的影响。沉积物的粒度是控制表层沉积物重金属分布的最主要因素,次要的因素包括沉积物有机质的含量、沉积速率以及重金属存在形态等。 2.首次获得了南黄海海水和沉积物中重金属的演变趋势 近10年来南黄海表层海水中,Zn呈上升趋势,As、Cd、Cu、Pb基本稳定变化不大,而Hg则呈略下降趋势。Zn的线性上升趋势明显,在近岸水域和中央水域中其浓度和公元年的统计关系分别为y=0.9524x+0.0034(R=0.97)和y=0.8622x+0.0299(R=0.95)(其中y为Zn的浓度,x为年度,取1997~2004)。近10年沉积物中重金属年际变化较小,浓度变化在多年均值的±(10%~30%)之间变动。As、Cd、Cu、Hg、Pb、Zn的均值变化范围分别为7.17±1.70、0.108±0.024、17.61±1.65、0.024±0.008、18.44±4.26、70.53±5.73 mg/kg,除了Hg随公元年呈较好的线性增加(y=0.0033x-6.50,R=0.75)外,其它重金属未显现出有明显的演变趋势。 近百年来,南黄海重金属的变化可分为3个阶段,20世纪60年代以前,20世纪60年代至90年代,及20世纪90年代至今。第一个阶段的60年可以看作是南黄海未明显受人类活动影响的一个时期,该段时期内明显的特征是重金属含量的变化受径流输入不均等多种因素影响,变化规律性不强;第二阶段是南黄海近岸工农业迅猛发展的阶段,由近岸传输到这一海域的重金属量增加,南黄海沉积物重金属浓度增加,沉积物质量有下降的趋势,这一阶段是人类活动影响南黄海最为明显的一个阶段;第三个阶段是20世纪90年代至今,南黄海沉积物重金属浓度呈降低趋势,与中韩两国减排及治污措施有关。近几年,南黄海沉积物的环境质量较20世纪末期有了较明显的改善。 3. 初步阐明了南黄海重金属的环境污染危害和潜在生态风险 采用潜在生态危害指数法和地积累指数等方法对南黄海沉积环境进行分析,结果表明,中等重金属污染程度海区占研究海区面积的38.7%,中等生态风险的区域则占了研究海区面积77.8%,但均未发现沉积物中的重金属与生物量的分布有明显的关系,总体表明,南黄海沉积物中的重金的污染状况及生态风险较低,南黄海沉积物质量良好。
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扇贝养殖是我国重要的海水养殖产业,然而自1997 年以来,养殖扇贝陆续爆发的大规模死亡,不但造成了巨大的经济损失,而且严重影响了该产业的健康发展。丝氨酸蛋白酶抑制因子及丝氨酸蛋白酶在无脊椎动物的免疫应答中起着核心作用,它们的协同作用直接导致外界病源入侵的信号转导和级联放大,并进一步激活一系列防御体系,如黑化反应、血液凝结和抗菌肽的合成等。因此,克隆扇贝参与免疫防御的丝氨酸蛋白酶抑制剂基因并对其功能进行研究,将有助于进一步研究扇贝的免疫防御机制,丰富和发展无脊椎动物免疫学的内容。 运用大规模EST技术和RACE技术从栉孔扇贝中克隆出一个Kazal型丝氨酸蛋白酶抑制剂基因,定名为CfKZSPI。该基因cDNA序列全长1788bp,其中5' 非编码区(Untranslated Region, UTR)为97 bp,3' UTR161 bp,有一个典型的多聚腺苷酸信号序列(AATAAA)和一个ploy A 尾巴,开放阅读框(Open Reading Frame, ORF)含有1530 bp,编码509 个氨基酸残基。对其推测氨基酸序列进行分析,发现其中包括22个氨基酸残基组成的信号肽序列和12个Kazal型丝氨酸蛋白酶抑制剂结构域。采用QRT-PCR(quantitative real time PCR)对鳗弧菌浸泡刺激后栉孔扇贝血淋巴中CfKZSPI 的 mRNA表达量进行了检测,发现其mRNA 的表达量在鳗弧菌刺激后3h明显上升,达到空白组的43.6倍;然后在6h时有所下降,为空白组的15.0倍;随着菌刺激时间的增长,CfKZSPI基因的 mRNA 表达量急剧增加,在刺激后8h,12h,24h分别达到空白组的174.1,207.8,675.4倍。统计分析发现3h(P=0.019<0.05)和12h(P=0.020<0.05)时,CfKZSPI基因mRNA表达量与空白组差异均显著。为了研究栉孔扇贝CfKZSPI的蛋白活性,将其第十二个结构域克隆到pET-32a(+)载体中,转化大肠杆菌Rosetta-gami(DE3)表达菌株,获得可溶性表达的蛋白rCfKZSPI-12,对其进行抑制蛋白酶活性的分析,发现其对胰蛋白酶有很强的抑制活性,而对凝血酶没有抑制活性。当rCfKZSPI-12与胰蛋白酶分子比率为1:1时,约90%的蛋白酶活性被抑制。运用狄更斯作图法研究rCfKZSPI-12对胰蛋白酶的抑制能力,结果发现其对胰蛋白酶的抑制常数为173 nmol L-1。 采用同样方法从海湾扇贝cDNA文库中克隆出一个Kunitz型丝氨酸蛋白酶抑制剂基因,定名为Aikunitz。该基因全长632 bp,其中5' UTR 为105 bp,3' UTR 为 245 bp,有一个典型的多聚腺苷酸信号序列(AATAAA)和一个ploy A 尾巴,ORF 含有282 bp,编码93 个氨基酸残基。推测的氨基酸序列N末端有一个20个氨基酸残基组成的信号肽序列,成熟蛋白包括一个Kunitz型丝氨酸蛋白酶抑制剂结构域。采用QRT-PCR对鳗弧菌和藤黄微球菌感染后海湾扇贝血淋巴中Aikunitz 的mRNA的表达量进行了检测,结果发现其在鳗弧菌刺激后3h到9h持续上升,9h时表达量为PBS对照组的4.49倍(P=0.008<0.05),然后开始下降,在72h时表达量为对照组的0.24倍(P=0.021<0.05);而在藤黄微球菌刺激后3h到12h其表达量上升,其中6h时为空白组的5.95倍(P=0.0004<0.01);12h以后迅速下降,其中24h的表达量为对照组的0.38倍(P=0.028<0.05)。将Aikunitz基因编码的成熟蛋白按照重组CfKZSPI-12的方法进行重组表达,并对重组蛋白进行抑制蛋白酶和抑菌活性分析。结果发现其对胰蛋白酶和弹性蛋白酶两种丝氨酸蛋白酶都没有抑制作用。抑菌实验同样发现,重组Aikunitz 对供试的革兰氏阳性菌藤黄微球菌和革兰氏阴性菌鳗弧菌和大肠杆菌都不显示明显抑菌活性。
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利用国内首次引进的Nu Plasma型MC-ICPMS,对Nd同位素测试过程的质量歧视效应特征进行了研究.发现该类仪器测试过程的质量歧视效应并不完全符合指数函数,也不符合幂函数.质量歧视引起的同位素分馏随同位素对的平均质量数变化,但不严格遵从线性关系.只是在较小质量范围内呈近似线性关系.据此,用双分馏系数校正法可以获得143Nd/144Nd的校正系数,并用来校正143Nd/144Nd值.用此方法获得的JNdi-1的143Nd/144Nd值为O.512 107±O.OOO 008(2RSD),在测量误差范围内与TIMS测定值完全吻合.
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前人侧重于对墨江金矿床含金石英脉的地质、地球化学和形成作用的研究,而对其含矿硅质岩研究较少。深入研究含矿硅质岩的成因和沉积环境,对认识墨江金矿的成矿规律有重要作用。本文主要讨论墨江金矿床含矿硅质岩的稀土元素特征及其意义。