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首次报道了亚东鲑在青藏高原河流中的生长特点。对1999—2002年间在亚东河共采集的462尾亚东鲑(体长范围为93—364mm)进行了年龄与生长的研究。通过鳞片对其中302尾进行年龄鉴定并建立Von Bertalanffy生长方程:雌性(♀)Lt=709.44[1-e-0.1656(t-0.0845)](R2=0.9993,n=132)和Wt=6017.46[1-e-0.1656(t-0.0845)]2.9724(R2=0.9845,n=132);雄性(♂)Lt=797.82[1-e-0.1428(t-0

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国家自然科学基金(No.30070588;No.30371091)

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两观测者独立观测了分别采自洪湖的 1 75尾和洞庭湖的 1 6 8尾鲫样本的鳞片和耳石 ,结果显示鳞片适于鉴定年龄组成简单、生长较快的洞庭湖鲫种群的年龄 ,两观测者年轮读数的总吻合率可达 90 5% ,与耳石上年轮读数的吻合率也可高达 91 7% ;但用鳞片鉴定年龄结构复杂、生长缓慢的洪湖鲫种群年龄 ,两观测者总吻合率只有 50 9% ,各龄组吻合率随年龄上升而迅速下降 ,与耳石上年轮读数总吻合率也仅为 56 6 % ,存在比耳石低估高龄个体年龄的问题。用耳石鉴定鲫年龄具有易于识别、精确度高的优点 ,用之

淡水轮虫休眠卵的研究进展

本文综述了温度、休眠卵形态、光照条件和休眠卵贮存条件等因子对轮虫休眠卵萌发的影响, 以及休眠卵萌发型式差异的生态学意义。温度骤变可以诱导休眠卵萌发, 光照条件对休眠卵萌发率的影响存在种间差异, 母体轮虫的食物和休眠卵贮存条件影响其萌发率。

PEG诱导的柱胞鱼腥藻（Anabaenacylindrica）原生质球融合

以多盐复合液配制的ＰＥＧ溶液可诱导柱胞鱼腥藻原生质球融合，融合率约５％，融合时间６～４０ｍｉｎ，可发生两个或多个原生质球的融合，ＰＥＧ使用浓度１０％

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于1990年8月-1992年4月在汉阳县军江渔场采集鳜标本，运用形态学和行为学实验方法研究其侧线管的形态结构和捕食行为反应特性。结果表明，职眶上管、眶下管管腔和神经丘直径最大，部分管道未埋入骨组织中；躯干部侧线管管腔和神经丘直径最小，全部埋人鳞片中；前鳃盖下颌管、眼后管等头部侧线管管腔和神经丘直径介于前二者之间，全部埋入骨组织中。饭测线对头部前上方振动刺激很敏感并可诱导捕食反应；对头部侧面振动刺激反应不强，对躯干侧面和后方振动刺激仅有警戒反应。据此，认为鳜头上部位直径大、未完全理入骨组织中的侧线管感觉灵敏

单细胞绿藻的大量培养试验

<正> 在近廿年来,单细胞绿藻的培养和利用,曾引起广泛的兴趣,主要的是由于单细胞绿藻具有下列几个重要的特性:(1)能够充分利用太阳的光能,通过光合作用,制造大量的有机物质,作为动物的直接或简接的食料;(2)生长繁殖迅速,对自然环境的适应性很强,易于培养;(3)蛋白质的含量很高,达干重的50%,其中有21种氨基酸为动物生长上所需要的,油脂的含量也很高,为干重的10—20%,通过低氮培养,含量可达到80%以

Simultaneous determination of microcystin contaminations in various vertebrates (fish, turtle, duck and water bird) from a large eutrophic Chinese lake, Lake Taihu, with toxic Microcystis blooms

This is the first to conduct Simultaneous determination of microcystin (MC) contaminations in multi-groups of vertebrates (fish, turtle, duck and water bird) from Lake Taihu with Microcystis blooms. MCs (-RR, -YR, -LR) in Microcystis scum was 328 mu g g(-1) DW. MCs reached 235 mu g g(-1) DW in intestinal contents of phytoplanktivorous silver carp, but never exceeded 0.1 mu g g(-1) DW in intestinal contents of other animals. The highest MC content in liver of fish was in Carassius auratus (150 ng g(-1) DW), followed by silver carp and Culter ilishaeformis, whereas the lowest was in common carp (3 ng g(-1) DW). In livers of turtle, duck and water bird, MC content ranged from 18 to 30 ng g(-1) DW. High MC level was found in the gonad, egg yolk and egg white of Nycticorax nycticorax and Anas platyrhynchos, suggesting the potential effect of MCs on water bird and duck embryos. High MC contents were identified for the first time in the spleens of N. nycticorax and A. platyrhynchos (6.850 and 9.462 ng g(-1) DW, respectively), indicating a different organotropism of MCs in birds. Lakes with deaths of turtles or water birds in the literatures had a considerably higher MC content in both cyanobacteria and wildlife than Lake Taihu, indicating that toxicity of cyanobacteria may determine accumulation level of MCs and consequently fates of aquatic wildlife. (C) 2009 Elsevier B.V. All rights reserved.

Cloning and characterization of the gene for UDPGlc dehydrogenase from the cyanobacterium, Microcystis aeruginosa FACHB 905

Using degenerate primers based on conserved regions of the UDP-glucose dehydrogenase (UDPGDH) gene, an initial 476-bp DNA fragment was amplified from the water-bloom forming cyanobacterium, Microcystis aeruginosa FACHB 905. TAIL-PCR and ligation-mediated PCR were used to amplify the flanking regions to isolate an about 2.5-kb genomic DNA fragment. Sequence analysis revealed an ORF encoding a putative 462 amino acid protein, designated Mud for Microcystis UDPGDH. The Mud amino acid sequence is closely related to UDPGDH sequences from cyanobacterium Synechocystis PCC6803 (73% identity, 81% similarity), and bacterium Bacillus subtilis (51% identity and 67% similarity). The cloned mud gene was expressed in Escherichia coli using the pGEX-4T-1 fusion expression vector system to generate a GST-Mud fusion protein that exhibited UDPGDH activity. The cytosolic fraction of M aeruginosa FACHB 905 was subjected to Western analysis with an anti-Mud antibody, which revealed a single band of approximately 49 kD, consistent with the deduced molecular mass of the enzyme. The Mud protein could thus be characterized as a UDP-glucose dehydrogenase, which was a key enzyme for polysaccharide synthesis and has, for the first time, been studied in algae.

Marine Phallodrilinae (Oligochaeta, Tubificidae)of Hainan Island in southern China

Six species of Phallodrilinae are reported from Hainan Island in southern China. The mesopsammic Aktedrilus yiboi sp. nov. is new to science. It is characterized by a combination of (1) small, straight, conical, strongly cuticularized penis sheaths, (2) posterior prostate glands being associated with the most ectal parts of the atria, and (3) small spermathecal ampulla. Bathydrilus ampliductus Erseus, 1997 is recorded for the first time since it was originally described from Australia's Northern Territory.