Generic allocation of Indian and Sri Lankan Philautus (Anura: Rhacophoridae) inferred from 12S and 16S rRNA genes

The generic allocation of Indian and Sri Lankan Philautus needs further examination. In this study, a comprehensive understanding of the phylogeny of Indian and Sri Lankan Philautus is obtained based on 125 and 16S rRNA genes. All phylogenetic analyses indicate that Indian-Sri Lankan Philautus, Philautus menglaensis, Philautus longchuanensis, and Philautus gryllus form a well supported clade, separate from Philautus of Sunda Islands that form another well supported clade representing true Philautus. This result supports the designation of the genus Pseudophilautus to accommodate the Indian and Sri Lankan species. Pseudophilautus consists of two major lineages, one comprises the majority of Indian species, Chinese species, and Southeast Asian species, and one comprises all Sri Lankan species and a few Indian species. Pseudophilautus may have originated in South Asia and dispersed into Southeast Asia and China. Based on the results, we further suggest that Philautus cf. gryllus (MNHN1997.5460) belongs to the genus Kurixalus. (C) 2010 Published by Elsevier Ltd.

中国人精母细胞和卵母细胞联合复合体的电镜观察

列出中国人的SC核型和模式图。根据减数分裂前期XY的复杂形态变化, XY的配对可分为5种 类型。对XY短臂之间形成的SC和XY长臂顶端的次级联合以及XY 配对的性质和机理作了描述 和讨论。报道了一个罕见的三倍体精母细胞, 对三倍体精母细胞中SC的配对行为以及和人类染色体疾病病因的可能关系作了分析和讨论。图版2图1表1参19

人类基因组多样性保护与伦理学和法律问题初探

中国科学院基金

西南山区河流阶梯—深潭系统的生态学作用

山区河流发育的阶梯—深潭系统具有显著的生态学作用。阶梯—深潭系统增大水流阻力和河床抗冲刷力,稳定了河床和岸坡。大卵石堆积成阶梯,细颗粒泥沙在深潭河段的缓流滞流区沉积下来形成淤泥层,形成适宜多种生物的栖息地。选择小江支流———深沟、蒋家沟和小白泥沟,以及四川九寨沟和金沙江进行野外实验、取样分析。结果发现阶梯—深潭系统较发育的深沟和九寨沟底栖动物密度高达552个/m2,生物量高达5.96 g/m2。而邻近的小白泥沟和蒋家沟底栖动物密度仅0.75个/m2,生物量不到0.006 g/m2。考虑河流不同部位底质、水

噬菌体展示技术的原理和方法

中国科学院择优特别支持费 [Stz98-3-0 5 ]; 国家自然科学基金 [30 0 5 0 0 0 3H];[30 1 70 72 7]资助

蓝藻Anabaena sp.PCC7120 羧体碳酸酐酶的鉴定

在丝状蓝藻Anabaena sp.PCC7120细胞粗提液的碳酸酐酶(CA)分析中,发现了两种形式的CA活性.高CO_2下生长的细胞,在35μmol/L EZ(Ethoxyzolamide,碳酸酐酶的抑制剂)存在的情况下,CA总活性的85％左右被抑制,其半抑制浓度I_(50)为7.4μmol/L;随着EZ浓度的继续增加,CA活性在EZ浓度达到约150μmol/L处出现了第二个抑制峰,在250μmol/L处抑制程度达到最大,使CA总活性的15％被抑制,其半抑制浓度I_(50)为190μmol/L。在空气条件

鱼类生产力研究的方法与进展

国家自然科学基金(湖泊食鱼性鱼类生产潜力估算方法的研究 );396 70 575号;中国科学院资源与生态环境局资助(长江中下游浅水湖群比较?

蓝藻Anabaenasp.strainPCC7120中一种可诱导的CO_2浓缩机制(CCM)

为了探讨蓝藻Anabaenasp .strainPCC71 2 0在外源无机碳浓度变化时 ,其光合作用对CO2 和HCO-3 的利用特性 ,制备了高CO2 适应细胞 (High CO2 growingcells ,HCG细胞 ) .Anabaenasp .strainPCC71 2 0HCG细胞的生长速率高于LCG细胞 (Low CO2 GrowingCells) ,即在空气中生长的细胞 .当HCG细胞从 5 %CO2 转移到空气中时 ,其碳酸酐酶活性升高 ;它对外源无机碳的表观光合作用亲合力明显提高 ,

罗非鱼对微型生态系统营养物水平的影响

本文总结了罗非鱼不同放养密度的微型生态系统中N、P浓度及P分布动态观测结果。在罗非鱼的影响下,微型生态系统中氨氮、颗粒磷和总磷浓度不同程度地高于对照组,而正磷酸盐浓度和沉积物磷的量显著地低于对照组。不同密度组某些指标的观测值虽有显著差异,但未见任何指标依罗非鱼放养密度而有规律地变动。微型生态系统中正磷酸盐浓度同浮游动、植物密度和初级生产力显著相关,氨氮浓度与浮游植物密度之间亦有显著的相关关系。然而,浮游植物密度与总磷浓度之间不存在营养级联假说所预见的下行影响,相反有前者决定于后者的上行影响的趋向。微型生态

Studies on temporal and spatial variations of phytoplankton in Lake Chaohu

Temporal and spatial variations of the phytoplankton assemblage in Lake Chaohu, a large shallow eutrophic lake in China, were studied from September 2002 to August 2003. A total of 191 phytoplankton species was identified, among which Chlorophytes (101) ranked the first, followed by Cyanophytes (46) and Bacillariophytes (28). On average over the entire lake, the maximum total algal biomass appeared in June (19.70 mg/L) with a minimum (5.05 mg/ L) in November. In terms of annual mean biomass, cyanobacteria contributed 45.43% to total algal biomass, followed by Chlorophytes (27.14%), and Bacillariophytes (20.6%). When nitrate (NO3-N) and ammonium (NH4-N) concentrations dropped in spring, fixing-nitrogen cyanobacterium (Anabaena) developed quickly and ranked the first in terms of biomass in summer. It is likely that dominance of zooplanktivorous fish and small crustacean zooplankton favored the development of the inedible filamentous or colony forming cyanobacteria. The persistent dominance of cyanobacteria throughout all seasons may indicate a new tendency of the response of phytoplankton to eutrophication in Lake Chaohu.

Age and growth of an endemic cyprinid fish, Spinibarbus yunnanensis, from Lake Fuxian, China

Age and growth of Spinibarbus yunnanensis Tsu in Lake Fuxian were investigated by examination of annuli from scales, dorsal fin spines, and otoliths. Sectioned otoliths exhibited the clearest and the most regular annuli. The formation of false annuli, which regularly arose on scales and dorsal fin spines during the juvenile stage, may be caused by a change in diet during development. The parameters for the von Bertalanffy growth curve were: K = 0.105, L-infinity = 950 mm, t(0) = -0.22 year for both sexes. W-infinity, calculated from the relationship W-infinity = aL(infinity)(b), was 10,352 g.

Genome segment S8 of grass carp hemorrhage virus encodes a virion protein

The complete nucleotide sequence of the genome segment S8 of grass carp hemorrhage virus (GCHV) was determined from cDNA corresponding to the viral genomic RNA. It is 1,287 nucleotides in length and contains a large open reading frame that could encode a protein of 409 amino acids with a predicted molecular mass of 44 kD. The S8 was expressed using the pET fusion protein vector and detected by Western blotting analysis using the chicken egg IgY against intact GCHV particles, indicating that S8 encodes a virion protein. Amino acid sequence comparisons revealed that the protein encoded by S8 is closely related to protein alpha2 of mammalian reovirus, suggesting that the deduced protein of S8 is an inner capsid protein. Copyright (C) 2001 S. Karger AG, Basel.