配合物Ru(dcbpy)(phen)_2(PF_6)_2的2D-NMR分析

用一维ＮＭＲ方法研究了新型电化学发光探针Ru(dcbpy)(phen)2(PF6)2的立体结构，借助二维1H-1HCOSY和1H-13ccosy实验技术对其氢谱和碳谱进行了完全的归属，并给出了其氢谱和碳谱的化学位移值。

2D NMR and FT-Raman spectroscopic studies on the interaction of lanthanide ions and Ln-DTPA with phospholipid bilayers

The interactions of lanthanide ions and the Ln-DTPA (DTPA = diethylenetriaminepentaacetate) complex with di palmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) bilayers are studied by 2D NOESY and FT-Raman spectroscopy. Proton NMR spectroscopic results show that lanthanide ions combine with phosphate groups in the polar region of the outer layer of DPPC liposomes, leading to the separation in chemical shift of the proton signal of N(CH3)(3) The conformational change of the O-C-C-N+ backbone from the gauche conformer to the trans one is not found; i.e., the orientation of the polar headgroup is still parallel to the surface of the bilayers. The Ln-DTPA complex at low concentration in a pH 7.4 solution localizes far away from bilayers and thereby has little effect on the structure of bilayers. The FT-Raman spectroscopic results indicate that lanthanide ions affect strongly the fluidity of acyl chains of DPPE bilayers while the Ln-DTPA complex affects it slightly.

用A-PAGE鉴定谷子遗传多样性

蛋白质是结构基因编码的产物,其组成不因环境条件的改变而改变,可以作为对其编码的结构基因的标记.大量研究表明,禾谷类作物种子贮藏蛋白具有相当大的多态性,不同品种贮藏蛋白的电泳谱带存在较大差异,因此提供了一种表征基因型差异的有效而简便的方法