Preparation and characterization of multiwalled carbon nanotube-supported platinum for cathode catalysts of direct methanol fuel cells

Multiwalled carbon nanotube-supported Pt (Pt/MWNT) nanocomposites were prepared by both the aqueous solution reduction of a Pt salt (HCHO reduction) and the reduction of a Pt ion salt in ethylene glycol solution. For comparison, a Pt/XC-72 nanocomposite was also prepared by the EG method. The Pt/MWNT catalyst prepared by the EG method has a high and homogeneous dispersion of spherical Pt metal particles with a narrow particle-size distribution. TEM images show that the Pt particle size is in the range of 2-5 nm with a peak at 2.6 nm, which is consistent with 2.5 nm obtained from the XRD broadening calculation. Surface chemical modifications of MWNTs and water content in EG solvent are found to be the key factors in depositing Pt particles on MWNTs. In the case of the direct methanol fuel cell (DMFC) test, the Pt/MWNT catalyst prepared by EG reduction is slightly superior to the catalyst prepared by aqueous reduction and displays significantly higher performance than the Pt/XC-72 catalyst. These differences in catalytic performance between the MWNT-supported or the carbon black XC-72-supported catalysts are attributed to a greater dispersion of the supported Pt particles when the EG method is used, in contrast to aqueous HCHO reduction and to possible unique structural and higher electrical properties when contrasting MWNTs to carbon black XC-72 as a support.

Theoretical study on the ClO/ClO- system electron-transfer reactivity by the golden-rule

The structures, properties and electron transfer reactivity of the ClO/ClO- coupling system are studied in this paper at ab initio (UHF and UMP2) levels and the Density Functional Theory (DFT: UB3LYP, UB3P86, UB3PW91) levels employing 6311 + G(3df) basis set and on the basis of the Golden-rule of the time-dependent perturbation theory. Investigations indicate that the results obtained using the UB3LYP method employing 6-311 + G(3df) basis set is in excellent agreement with the experiment. For this coupling system, six stable coupling modes have been found which correspond to six different encounter complexes and denote six different electron transfer mechanism: four O-O directly linked structures (one collinear: D-h, one anti-parallel: C-s, two twist: C-2) and two Cl-O linked structures (cis- and anti- C-s structures). The activation energies, the stabilization energies and the electronic coupling matrix elements have also been calculated for the electron transfer reactions via these six different mechanism at the UB3LYP/6-311 + G(3df) level, and then the electron transfer rates are determined at the same level. The most favorable coupling mode to the electron transfer is the anti-parallel mechanism. The averaged electron transfer rate is about 5.58 X 10(11) M-1 s(-1). It is also implied that the B3LYP method can give more reasonable results for the electron transfer reactivity of this system. (C) 2003 Elsevier B.V. All rights reserved.

Simultaneous determination of methylene blue and new methylene blue by slab optical waveguide spectroscopy and artificial neural networks

A slab optical waveguide (SOWG) has been used for study of adsorption of both methylene blue (MB) and new methylene blue (NMB) in liquid-solid interface. Adsorption characteristics of MB and NMB on both bare SOWG and silanized SOWG by octadecyltrichlorosilane (ODS) were compared. The simultaneous determinations of both MB and NMB were explored by flow injection SOWG spectrophotometric analysis and artificial neural networks (ANNs) for the first time. Concentrations of MB and NMB were estimated simultaneously with the ANNs. Results obtained with SOWG were compared with those got by conventional UV-visible spectrophotometry. (C) 2003 Elsevier Science B.V All rights reserved.

Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix

A novel protocol has been established to separate dsDNA fragments with high efficiency on glass chips by using an ultralow viscosity sieving matrix with added glucose. Low-molecular-weight hydroxypropylmethylcellulose (HPMC), with a viscosity nearly equivalent to that of water, was used to electrophoretically separate fluorescent inter-calator-labeled double-stranded DNA (dsDNA) fragments on microfluidic glass chips. In comparison with conventional sieving protocols, low-molecular-weight HPMC as sieving matrix could result in reduced running cost and analysis time, in addition to a comparable separation efficiency of dsDNA fragments. In this paper, the addition of glucose was investigated to enhance the separation of DNA in the lowest viscosity polymer evaluated. The effect of staining dye and field strength were also evaluated. At an applied electric field strength of 200 V/cm, satisfactory resolution of the PBR322/HaeIII DNA marker could be achieved within 4 min by using 2% HPMC-5 with 6% glucose added. Coelectrophoresing PCR product along with phiX174/HaeIII DNA sizing marker was also demonstrated by using the ultralow viscosity HPMC-5 solution on a glass chip.

几种伴生植物水浸液对杉木生长的影响研究

用8种杉木伴生植物及杉木的茎和叶不同浓度水平(水平Ⅰ为166.7g/kg,水平Ⅱ83.3g/kg,水平Ⅲ41.7g/kg·水平Ⅳ20.8g/kg)的水浸液浇灌盆栽杉本幼苗,连续观测6年结果表明8种植物水浸液浇灌后杉木高度与地径比对照Ⅰ(浇灌杉木自身水浸液)和对照Ⅱ(仅浇灌清水)均有不同程度提高。不同浓度水浸液对杉木高度和地径的促进作用依次为水平Ⅰ>水平Ⅱ>水平Ⅲ>水平Ⅳ>对照Ⅱ,不同植物水浸液对杉木株高的促进作用则为木荷>檵木>椤木石楠>狗脊>丝粟栲>赤楠>马尾松>芒萁>杉木(对照Ⅰ)

林内穿透雨量模型研究

穿透雨量是林内有效雨量的主要组成部分 ,其量能占 90 %以上。由于林冠空间分布的不均匀性 ,难以对林内穿透雨量进行精确地测量。从林冠截留降雨的作用机理出发 ,在做出几个假设的基础上 ,用数学方法建立了林内穿透雨量模型 ,即 :当 P0 ,U>0 )当 P≥ U时 ,R=P- W其中 U=W( b+ 1 ) ,式中 b,W,U为模型参数 ,R为林内穿透雨量 ,P为大气降雨量 ,α为林分郁闭度。结合两个有实测资料的针叶树林分 ,又进一步介绍了模型参数的确定方法。同时将模型的计算结果与实测数据进行了对比 ,两者吻合性很好 ,验证效果是比较理想的。模型概念清晰 ,参数物理意义明确。在风速和雨强不太大的情况下 ,本模型适用于任何林分的穿透雨量的计算。然而 ,为了应用于无实测资料地区 ,尚须进一步研究模型参数与林分特征的关系。

杉木经几种源植物水浸液处理后叶绿素、质膜透性及气孔的变化研究

用8种源植物及杉木枝叶不同浓度水浸液和清水浇灌盆栽杉木幼苗试验观测结果表明,经几种源植物水浸液处理后杉木叶绿素含量、叶片气孔孔径及其大小均比对照明显提高,叶片质膜透性则明显降低,但气孔频数无显著影响,其中木荷对提高叶绿素含量和降低质膜透性效果最佳,与杉木对照(CK_Ⅰ)和清水(CK_Ⅱ)处理相比,木荷1:6水浸液处理的杉木叶绿素含量分别提高49.3％和52.5％,而质膜透性分别下降30.6％和26.3％。檵木对提高杉木叶片气孔孔径及其大小效果最佳,与清水(CK_Ⅱ)处理相比分别提高27.1％和30.3％。水浸液浓度越大,其叶绿素含量、气孔孔径及其大小越大,则质膜透性越低。

生物多样性管理最小有效面积选取模型与GIS的耦合(英文)

人口的增长和自然资源的有限性决定了很难把更多的土地用于生物多样性的保护。通过 BMAS(生物多样性管理面积选取 )模型与 GIS(地理信息系统 )的结合 ,可以用尽可能少的土地资源实现一定水平的生物多样性的保护。该方法初步在西双版纳的勐拉县进行了应用。与现实自然保护区面积的对比 ,用模型选区自然保护区有显著效果。该方法在保护与发展矛盾尖锐的发展中国家非常适用。

塔里木沙漠公路防护林土壤微生物活性研究

对不同立地类型、不同生长年限、不同季节、不同树种的防护林土壤微生物数量及区系组成进行研究,分析了土壤微生物与土壤环境因子的相关关系,结果表明:咸水灌溉条件下塔里木沙漠公路防护林和人工绿地建成后,土壤结构和养分状况得到改善,微生物活性大幅度提高;土壤微生物区系组成中,细菌占绝对优势,放线菌次之,真菌最少;表层微生物数量远多于下层土壤;林地外围距林缘近的土壤微生物活性较强,3m以外微生物数量已接近流沙地微生物量;各类土壤微生物分布具不同的季节性规律,且不同立地类型防护林土壤微生物分布差异明显;随造林年限的增加,防护林的改土作用更加显著。土壤微生物分布与土壤养分含量和土壤pH值显著相关。

一种处理高浓度二氧化氮废气的方法及其设备

一种处理高浓度二氧化氮废气的方法及其设备，以液化气为燃料，点燃该燃料之后，通入１０－１００％体积百分比浓度的二氧化氮气体一同燃烧，生成水和二氧化碳和氮气，达到处理氮氧化物的目的。具有工艺简单、操作容易、设备简单、燃烧后尾气中的二氧化氮残余量小于６００ｐｐｍ。

Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta)

Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated with plasmid DNA containing the lacZ reporter gene. Transient expression of lacZ was observed in bombarded thalli under the rupture-disc pressures of 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. changii exhibits a slight blue background, positive expression and the background colour can be clearly differentiated. The results indicate that lacZ could be a useful reporter gene and that SV40 promoter could be an effective promoter for Gracilaria transformation.

Chandrananimycins A-C: Production of novel anticancer antibiotics from a marine Actinomadura sp isolate M048 by variation of medium composition and growth conditions

In our screening of marine actinomycetes for bioactive principles, three novel antibiotics designated as chandrananimycin A (3c), B (3d) and C (4) were isolated from the culture broth of a marine Actinomadura sp. isolate M045. The structures of the new antibiotics were determined by detailed interpretation of mass, 1 D and 2 D NMR spectra.