Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum

The prophenoloxidase(ProPO) gene was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends (RACE) method. The full-length cDNA of prophenoloxidase gene consists of 3040 bp with a 2061 bp Open Reading Frame (ORF), encoding 686 amino acids. Phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. To understand ProPO reaction for pathogeny's challenge in shrimp, the expressions of ProPO in different tissues were studied by real-time PCR after challenged by Vibrio anguillarum. The results showed that the expression level of ProPO gene in haemocytes was highest among three studied tissues including haemocytes, lymphoid organ and hepatopancreas. The time-course change of ProPO mRNA levels in challenge experiment showed that ProPO mRNA transcripts had the biggest change extent in lymphoid organ.

小麦体细胞无性系HMW-GS变异及其变异体的研究

关于小麦体细胞无性系变异前人已做了大量的研究,观察到了形态学、细胞学和生化特性变异.对醇溶蛋白和谷蛋白变异的分析,获得了基因突变的证据,但不知突变基因的位点.本研究的目的在于分析体细胞无性系的高分子量谷蛋白亚基(high-molecular-weightglutenin subunits,HMW-GS)的变化,获取特定基因(Glu-l)突变的证据,并研究HMW-GS变异体农艺性状和产量性状的表现.

供应链中物流及信息流管理

供应链管理使企业在变化的市场环境中有效地与其它企业合作 ,取得集体竞争优势。本文首先讨论了后勤学与供应链管理的定义和之间的关系。本文认为供应链管理的核心是物流与信息流的控制。物流控制决策主要包括操作层次的库存补充和运输路径规划 ,以及战略层次的设施地点规划。信息流管理跨越部门与企业的界限将相关的应用集成起来。动态联盟协调各企业内部的生产经营活动 ,战略性地决定物流与信息流的构形。

贵阳市表层土壤中铅的环境地球化学基线研究

以土壤环境地球化学研究为主线，以贵州省贵阳市8 064 km2 为研究区域，将土壤重金属污染元素铅的空间分布规律与环境地球化学机理研究相结合，建立区域土壤环境地球化学基线，选用合适的判别指标判识自然作用过程与人类活动过程对土壤环境的影响。得到以下结论：贵阳市表层土壤中铅的基线值为14.8 mg·kg-1，样品中铅元素含量大于70.1mg·kg-1 的样品可能遭受人为污染的影响。地质累积指数分析结果显示贵阳市18%的表层土壤未受铅的污染，47%的表层土壤在无污染到中度污染之间，28%的中度污染，6%的表层土壤介于中度污染到强污染之间，1%的强污染。污染程度指数分析则显示贵阳市49.9%的表层土壤未受到铅的污染，铅的污染程度最大为8.11，总污染程度略大于0，即受到轻微污染。