Distribution pattern of marine flagellate and its controlling factors in the central and north part of the Huanghai Sea in early summer

A survey was carried out in the central and north part of the Huanghai Sea (34.5degrees similar to 37.0degreesN, 120.5degrees similar to124.0degreesE) during June 12 similar to 27, 2000. It was found that the abundance of marine flagellate ranged from 45 to 1278 cell/ml, 479 cell/ml in average. Flagellate was more abundant in the central part than in the north part of Huanghai Sea, and the abundance decreased with the increasing distance from the coast, showing a similar distribution pattern with isotherm. Vertically, high density of flagellate was always presented in the bottom of thermocline, and formed a dense accumulation in the central area of the Huanghai Sea Cold Water Mass. The effects of physical and biological factors on the distribution of marine flagellate in early summer were discussed. Water temperature (especially the existence of thermocline) rather than salinity showed significant effect on the distribution pattern of marine flagellate in the Huanghai Sea in early summer. When comparing the abundance of marine flagellate with that of other microorganisms, it revealed a comparatively stable relationship among these organhisms, with a ratio of heterotrophic bacteria: cyanobacteria: flagellate: dinoflagellate: ciliate being 10(5) 10(3):10(2):10(1):10(0).

Density-dependent effects on seston dynamics and rates of filtering and biodeposition of the suspension-cultured scallop Chlamys farreri in a eutrophic bay (northern China): An experimental study in semi-in situ flow-through systems

Effects of stocking density on seston dynamics and filtering and biodeposition by the suspension-cultured Zhikong scallop Chlamys farreri Jones et Preston in a eutrophic bay (Sishili Bay, northern China), were determined in a 3-month semi-field experiment with continuous flow-through seawater from the bay. Results showed that the presence of the scallops could strongly decrease seston and chlorophyll a concentrations in the water column. Moreover, in a limited water column, increasing scallop density could cause seston depletion due to scallop's filtering and biodeposition process, and impair scallop growth. Both filtration rate and biodeposition rate of C. farreri showed significant negative correlation with their density and positive relationship with seston concentration. Calculation predicts that the daily removal of suspended matter from water column by the scallops in Sishili Bay ecosystem can be as high as 45% of the total suspended matter; and the daily production of biodeposits by the scallops in early summer in farming zone may amount to 7.78 g m(-2), with daily C, N and P biodeposition rates of 3.06 x 10(-1), 3.86 x 10(-2) and 9.80 x 10(-3) g m(-2), respectively. The filtering and biodeposition by suspension-cultured scallops could substantially enhance the deposition of total suspended particulate material, suppress accumulation of particulate organic matter in water column, and increase the flux of C, N and P to benthos, strongly enhancing pelagic-benthic coupling. It was suggested that the filtering-biodeposition process by intensively suspension-cultured bivalve filter-feeders could exert strong top-down control on phytoplankton biomass and other suspended particulate material in coastal ecosystems. This study also indicated that commercially suspension-cultured bivalves may simultaneously and potentially aid in mitigating eutrophication pressures on coastal ecosystems subject to anthropogenic N and P loadings, serving as a eutrophic-environment bioremediator. The ecological services (e.g. filtering capacity, top-down control, and benthic-pelagic coupling) functioned by extractive bivalve aquaculture should be emphasized in coastal ecosystems. (c) 2005 Elsevier B.V. All rights reserved.

Community structure and seasonal dynamics of planktonic ciliates along salinity gradients

The ciliate community structure and seasonal dynamics in a solar saltern of the Yellow Sea were studied based oil 4 sampling dates and 8 stations with salinities from 27.7 parts per thousand to 311.0 parts per thousand. The effects of the type and concentration of the fixative used (Lugol's and Bouin's) were tested at the first sampling date. Fixative type and fixative concentration had significant effects on ciliate abundance and blovolume, with 1% Lugol's giving the best results. A detailed investigation using live observations and protargol staining techniques revealed a total of 98 morphospecies from 8 sampling stations. There was obvious seasonal variation in species composition at most of the stations, but this tended to be less distinct with increasing salinity, as the dominant ciliate group shifted from oligotrichs to heterotrichs. Ciliate abundance varied from 4.40 x 10(1) to 2.11 x 10(5) cells l(-1) and biomass ranged between 2.39 and 9.87 x 10(3) mu g Cl-1 (at a salinity of 147.6 parts per thousand). Both abundance and biomass decreased abruptly when salinity exceeded 100-150 parts per thousand. Statistical analyses Suggested that the dynamics of ciliate abundance and biomass were regulated by both salinity and by season, but those of diversity and species richness were mainly controlled by salinity and both significantly decreased with increasing salinity. (C) 2009 Elsevier GmbH. All rights reserved.

青海湖区东部沙地植被及其特征研究

根据野外样地调查资料,对青海湖区东部沙地植被从区系特征及其物种多样性进行了研究,结果表明:①青海湖东部沙地植被种类组成简单,共有27科58属71种.其中禾本科比重最高为22.535%,其次为菊科占12.676%;再次为豆科占9.8592%;桔梗科等14科比重较小均占1.4085%.②地理成分上,湖东沙地植物区系以世界分布为主,共有16科,湖区植物区系中的优势科均为世界广布科;其次是温带成分10科;热带亚热带成分仅有1科.③生活型以多年生地面芽植物和地下芽植物为主,两者种类系数之和达到76.06%,一年生植物所占种类比重最小.④湖东沙地植被群丛多以单一优势种为主,物种多样性指数H、丰富度指数R1和均匀度指数Jsw三者的变化趋势大致是一致的,即随物种的增多,他们的指数趋向增大;生态优势度C的变化趋势则正好相反.⑤湖东沙地群丛物种相似性差异较大.固定沙地之间植被群丛相似性较高,流动半流动沙地与流动沙地之间则较低.

大鸨生长期能量代谢和蛋白质沉积量的初步研究

对不同日龄笼养大鸨(Otis tarda)的能量代谢和蛋白质沉积量进行研究.结果表明,50日龄、70日龄、300日龄和400日龄雄性大鸨的能量日摄入量分别为1 498.9 kJ、2 376.2 kJ、2 397.4 kJ和2 465.9 kJ;能量代谢率分别为82.3%、81.8%、81.8%和83.1%;蛋白质的日沉积量分别为5.8 g、13.4 g、15.8 g和13.5 g. 表3 参9

CHINESE NITRARIA TANGUTORUM BOBR。： CHEMICAL CONSTITUENTS OF SEED OIL EXTRACTED BY SFE-CO_2

In order to develop and make good use of Nitraria tangutorum Bobr. in Qinghai-Tibetan Plateau for its ecological and medicinal values, the seed oil was extracted by SFE-CQ2 and the chemical constituents was analyzed by GC/MS. The component relative contents were determined by area nomalization. 28 components were separated from the extracts of SFE-CQj and 12 of them, which accounted for 85.99% were identified. They were(Z, Z)-9, 12-octadecadienoic acid (linoleic acid), bicyclo[ 10. 1. 0] tridec-1-ene, 7-pentadecyne, gamma-sitosterol, gamma-tocopherol, 1, -8,Z-10-hexadecatriene,9,12-octadecadienal, 24-methyl-5-cholestene-3-ol,(Z)-9,17-octadecadienal, stigmastan-3,5-dien, eicosane and so on. Among them, the relative content of (Z, Z)-9,12-octadecadienoic acid is the highest, accounting for 65.85% of the total area. It is concluded that N. tangutorum Bobr. seed oil is a rich source of linoleic acid.

单宁酸对根田鼠食物摄入量和蛋白质消化率的效应

在食物中含10％和20％蛋白质的条件下，采用食物平衡法测定了单宁酸对根田鼠食物摄入量和蛋白质消化率的作用。食物蛋白质含量为10％时，第1-5天，单宁酸对根田鼠食物摄入量具有显著的抑制作用，自第6天，其作用不明显，以3％和6％单宁酸处理的根田鼠，其食物蛋白质消化率较对照组分别降低22％和47.67％；在食物蛋白质含量为20％的条件下，单宁酸对根田鼠的食物摄入量和蛋白质消化率无显著作用。上述结果验证了植物次生化合物能抑制植食性小哺乳动物食摄入量及蛋白质消化率的假设。

生物措施对柴达木盆地弃耕盐碱地效应分析

利用生物技术改良柴达木盆地弃耕盐碱地，3年结果表明，全盐量随着苜蓿种植年限的增加而降低。0～30cm耕作层全盐量由试验前的1.518%下降到0.126%，脱盐率在91.7%，盐分表聚现象已不明显。土壤盐分季节变化呈U型曲线，建植人工草地后，初级生产力明显提高。试验区植物群落的物种数、多样性指数、均匀度均高于对照区。

海北高寒草甸地区太阳总辐射、植被反射辐射的有关特征

祁连山海北高寒草甸地区，有较强的太阳总辐射及地表反射辐射。通过2000年观测结果表明，在植物生长期的4～10月，太阳总辐射和地表反辐射总量分别达4227.049MJ·m~(-2)和973.556MJ·m~(-2)。二者有明显的日变化规律，瞬时最高值出现于北京时间13～14h左右，日瞬时最大值分别可达1200W·m~(-2)和220W·m~(-2)以上。植物生长期间太阳总辐射变化受太阳高度角及其当地气候环境的影响，太阳高度角高或气候干燥时太阳总辐射值大，而地表反辅射依太阳总辐射的变化而变化，二者均在7月最高，5月次高。植物生长后期，气候湿润，太阳总辐射和地表反射辐射均较低。地表反射率不论在日间还是植物生长的季节间，均表现一“U”型变化过程，其中日间在中午前后最低，最低可达0.19季节变化以6～7月最低，约为0.21,当然受土壤潮湿程度及地表性质等影响，其它时间也可降到0.21。就整个植物生长期内来看，祁连山海北高寒草甸地区地表反射率平均约为0.23。

高施氮量对高寒矮嵩草甸主要类群和多样性及质量的影响

研究高寒矮嵩草草甸高强度施氮试验（150和300kg N/hm~2）对不同植物类群的影响。结果表明，禾草类植物的反应较大，其综合优势比呈明显增大趋势，非豆科杂草次之，莎草类和豆科杂草的反应不显著。增施氮肥能明显降低矮嵩草草甸的植物多样性，其草甸质量2指数以施氮150kg/hm~2最高，过量施氮（300/hm~2）并不再提高草甸质量。其地上生物量对施氮的反应比多样性和草甸质量指数的变化滞后，地上生物总量在施氮后明显增加，其中禾草类明显增加，杂类草则明显降低。7月的降水量及其分布对施氮的效果影响较大。土壤水分状况对施肥效果十分重要。

雪隆胶囊的质量标准研究

目的　制订雪隆胶囊的质量标准。方法　对雪莲花进行了薄层色谱鉴别 ;用分光光度法测定了 L -羟脯氨酸的含量。结果　含量测定平均回收率 10 1.1% (RSD=1.7% ,n=6 )。结论　该方法稳定 ,专属性强 ,可作为该制剂的质量控制方法。

高寒草甸藏嵩草种群繁殖对策的研究

对高寒草甸藏嵩草 (Kobresiatibetica)种群的繁殖对策进行了初步研究 .结果表明 :藏嵩草属寒冷中生密丛短根茎地下芽植物 ,在高寒生境中采用了以营养繁殖为主、有性繁殖为辅的繁殖策略 ,具体体现在以下几个方面 :藏嵩草种子产量nA=2 0 0 .1m-2 ,但种子萌发率较低 ,室内和野外萌发率分别仅有 4%和 2 % ,经氢氧化钠溶液和赤霉素溶液处理后的种子萌发率分别为 1%和 6 .7% ,而剥去种皮后种子萌发率达 47.3% ,所以种皮坚硬是造成种子萌发率低的主要原因 ;进入种子库、保留至返青期且具有活性的种子仅占种子总数的 31.49% ;单位面积上理论实生苗数仅为1.2 6m-2 ,与此相反 ,藏嵩草营养繁殖所形成的新个体数为 10 1.32m2 ,远远多于种子萌发所形成的实生苗数 .此外 ,藏嵩草营养繁殖效力也远高于有性繁殖效力，营养繁殖效力占总繁殖效力的83.46%（167）。

高寒牧区三种豆科牧草与燕麦混播的试验研究

在中国科学院海北高寒草甸生态系统开放实验站进行的燕麦与箭舌豌豆、红豆草、毛苕子3 种豆科牧草混播栽培的试验研究, 采用正交设计及极差分析方法对混播组合作择优评判。结果表明: 红豆草在年均温-1. 7 ℃, ≥5 ℃积温1 000 ℃, 海拔3 200 m 的高寒地区可与燕麦建立一年生混播割草地。其产量水平高于毛苕子与燕麦混播的草地而与箭舌豌豆与燕麦混播的草地相当。对豆科牧草种(A )、混作方式(B)、混播总密度(C) 和混播比例(D) 4 个因素进行择优组合可提高单位面积牧草产量。4 种试验因素对混播草地牧草产量的效应大小依次为C> A > D> B。本试验最优组合为5 号组合(A 2B2C3D1)。

气候变暖对高寒草甸气候生产潜力的影响

利用多年定位测定的草地生产力资料及同步气象因素观测数据, 建立现实状况下光、温、水影响草地气候生产潜力模型: 　　Y= F1 (Q ) ·F2 (T ) ·F3 (P) 　　 = K ∑[(-b·exp (a+ b∑t/∑tmax) )/(1+ exp (a+ b∑t/∑tmax) )2 ] 0　　　　　　　　　　　T < 0℃ ·[W 0/∑tmax ]·△∑t· Tö2 0·016658　　　0℃≤T< 20℃ 1　　　　　　　　　　　T ≥20℃ 在分析现实高寒草旬气候生产力分布与环境条件关系的同时, 根据该模型模拟计算未来气温升高2℃和4℃, 降水增加10% 和20% 状况下的气候生产力情景。在上述两种气候情景下, 未来草地生产力分别出现降低(10% ) 和升高(1% ) 的两种可能。气候变暖在一定程度上减少和缓和低温对高寒草甸牧草生长的不利影响, 但地表及植被的蒸散量的加大远比降水增加的快, 水分则成为牧草生长的限制因素。

CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides

A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column.