16 resultados para truncated regression
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Academic Research Repository at Institute of Developing Economies (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (1)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (1)
- Applied Math and Science Education Repository - Washington - USA (2)
- Aquatic Commons (4)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (44)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (28)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (37)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (57)
- Bulgarian Digital Mathematics Library at IMI-BAS (11)
- Cambridge University Engineering Department Publications Database (70)
- CentAUR: Central Archive University of Reading - UK (60)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (16)
- Cochin University of Science & Technology (CUSAT), India (9)
- Collection Of Biostatistics Research Archive (24)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (3)
- Dalarna University College Electronic Archive (2)
- Deakin Research Online - Australia (75)
- Department of Computer Science E-Repository - King's College London, Strand, London (2)
- Digital Commons - Michigan Tech (2)
- Digital Commons - Montana Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (4)
- Digital Knowledge Repository of Central Drug Research Institute (1)
- Digital Peer Publishing (2)
- DigitalCommons@The Texas Medical Center (25)
- DigitalCommons@University of Nebraska - Lincoln (2)
- Duke University (7)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (2)
- Indian Institute of Science - Bangalore - Índia (40)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Politécnico do Porto, Portugal (2)
- Massachusetts Institute of Technology (3)
- Ministerio de Cultura, Spain (4)
- National Center for Biotechnology Information - NCBI (34)
- Nottingham eTheses (2)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (53)
- Queensland University of Technology - ePrints Archive (89)
- Repositorio Academico Digital UANL (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (6)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (81)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- School of Medicine, Washington University, United States (1)
- Scientific Open-access Literature Archive and Repository (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (4)
- Universidad Politécnica de Madrid (16)
- Universidade Federal do Rio Grande do Norte (UFRN) (1)
- Universitat de Girona, Spain (4)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (3)
- Université de Montréal, Canada (10)
- University of Canberra Research Repository - Australia (2)
- University of Connecticut - USA (3)
- University of Michigan (9)
- University of Queensland eSpace - Australia (25)
- University of Southampton, United Kingdom (4)
- University of Washington (1)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
Mature human interleukin-11 (HuIL-11) is a cytokine consisting of 178 amino acid residues that results from scission of the N-terminal signal peptide, consisting of 21 amino acid residaues, from the corresponding nascent polypeptide. A DNA fragment encoding a truncated HuIL-11 (trHuIL-11), with an additional 5 amino acid residues removed from the N-terminus, was cloned into vector pGEX-2T between the BamHI site and the EcoRI site. Upon transformation with Escherichia coli BL21, the construct over-produced a glutathione S-transferase (GST)-fused protein in a soluble form after IPTG induction. The fusion protein was initially fractionated with butyl-Sepharose 4 fast flow column and by affinity chromatography using a GSH-Sepharose 4B column. On-site enzymatic release with thrombin gave the target protein at 96% purity as judged by SDS-PAGE and HPLC. Expression of the interleukin as a GST-fused protein thus greatly improved downstream processing. Subsequent biological activity assay suggested that trHuIL-11 had similar activity profile to the naturally produced sample and may be a promising candidate for further development as biopharmaceutical.