Polishable amperometric hydrogen peroxide sensor based on sol-gel-derived conductive composite containing vanadium-17-molybdodiphosphate

A new kind of conductive vanadium-17-molybdodiphosphate/graphite/methylsilicate composite was firstly prepared by the sol-gel technique and used as electrode material for the fabrication of amperometric hydrogen peroxide sensor. The remarkable advantage of the sensor is its excellent reproducibility of surface renewal by simple mechanical polishing.

Renewable-surface amperometric nitrite sensor based on sol-gel-derived silicomolybdate-methylsilicate-graphite composite material

A new type of silicomolybdate-methylsilicate-graphite composite material was prepared by the sol-gel technique and used for the fabrication of an amperometric nitrite sensor. The silicomolybdic anion acts as a catalyst, the graphite powder ensures conductivity by percolation, the silicate provides a rigid porous backbone and the methyl groups endow hydrophobicity and thus limit the wetting section of the modified electrode. Cyclic voltammetry, square-wave voltammetry and chronoamperometry were employed to characterize the sensor. The amperometric nitrite sensor exhibited a series of good properties: high sensitivity (1.771 mu A mmol(-1) dm(3)), a short response time (7 s), remarkable long-term stability and especially reproducibility of surface renewal in the event of electrode surface fouling.

A new kind of potassium sensor based on capacitance measurement of mimic membrane

A novel type of potassium sensor based on the capacitance change of valinomycin-incorporated bilayer supported on a gold electrode has been developed and characterized. The lipid membrane was Formed by painted method and monitored simultaneously by capacitance variation. The capacitance of the electrode-supported membrane was found to be modulated by different concentrations of K+. Investigating the capacitance change allows a simple and specific technique for the measurement of potassium ion in solution. Especially, the homemade capacitance meter is, to our knowledge, used to monitor the bilayer membrane formation and detect K+ for the first time. It has been proved that this capacitance measurement is a very useful technique because it is simple and sensitive compared to the other methods.

CHOLESTEROL SENSOR-BASED ON ELECTRODEPOSITION OF CATALYTIC PALLADIUM PARTICLES

A layer of palladium particles was electrodeposited on a glassy carbon electrode. The dispersed Pd particles resulted in a large decrease in overvoltage for the electrochemical oxidation of H2O2 down to +0.4 V vs. Ag/AgCl, based on which a new kind of cholesterol sensor was fabricated. Cholesterol oxidase was immobilized on the Pd-dispersed electrode by cross-linking with glutaraldehyde and a layer of poly(o-phenylenediamine) (PPD) film was electropolymerized on the enzyme layer. The sensor shows a linear response in the concentration range 0.05-4.50 mmol l-1 with a rapid response of less than 20 s. The polymer film can prevent interference from uric acid and ascorbic acid and also increases the thermal stability of the sensor. The sensor can be used for 200 assays without an obvious decrease in activity.

FLOW-INJECTION ANALYSIS OF GLUCOSE AT AN AMPEROMETRIC GLUCOSE SENSOR-BASED ON ELECTROCHEMICAL CODEPOSITION OF PALLADIUM AND GLUCOSE-OXIDASE ON A GLASSY-CARBON ELECTRODE

A glassy carbon electrode (GCE) modified with palladium provides excellent electrocatalytic oxidation of hydrogen peroxide. When the electrolyte contains palladium chloride and glucose oxidase, the GCE can be modified by electrochemical codeposition at a given potential. The resulting modified surface was coated with a thin film of Nation to form a glucose sensor. Such a glucose sensor was successfully used in the flow-injection analysis of glucose with high stability and anti-poisoning ability. It gave a detection limit of 1 X 10(-7) M injected glucose, with a linear concentration range of 0.001-8 mM. There is no obvious interference from substances such as ascorbate and saccharides.

An electrochemical DNA sensor based on electrodepositing aluminum ion films on stearic acid-modified carbon paste electrode and its application for the detection of specific sequences related to bar gene and CP4 Epsps gene

An electrochemical DNA biosensor was fabricated by immobilizing DNA probe on aluminum ion films that were electrodeposited on the surface of the stearic acid-modified carbon paste electrode (CPE). DNA immobilization and hybridization were characterized with cyclic voltammetry (CV) by using methylene blue (MB) as indicator. MB has a couple of well-defined voltammetric redox peaks at the CPE. The currents of redox peaks of MB decreased after depositing aluminum ion films on the CPE (Al(III)/CPE) and increased dramatically after immobilizing DNA probe (ssDNA/Al(III)/CPE). Hybridization of DNA probe led to a marked decrease of the peak currents of MB, which can be used to detect the target single-stranded DNA. The conditions for the preparation of Al(III)/CPE, and DNA immobilization and hybridization were optimized. The specific sequences related to bar transgene in the transgenic corn and the PCR amplification of CP4 epsps gene from the sample of transgenic roundup ready soybean were detected by differential pulse voltammetry (DPV) with this new electrochemical DNA biosensor. The difference between the peak currents of MB at ssDNA/Al(III)/CPE and that at hybridization DNA modified electrode (dsDNA/Al(III)/CPE) was applied to determine the Specific sequence related to the target bar gene with the dynamic range comprised between 1.0 X 10(-7) mol/L to 1.0 x 10(-4) mol/L. A detection limit of 2.25 x.10(-8) mol/L. of oligonucleotides can be estimated.

基于多传感器的可变形机器人控制方法研究

随着机器人技术的不断发展，机器人能力不断提高，其应用的领域和范围正在不断扩展，各式各样的机器人正逐步走入各行各业和社会的各个领域，并在其中发挥着越来越重要的作用，特别是在科学考察、军事侦察、灾难救援等对人类来讲极度危险复杂的领域。它所具有的优势越来越受到世界各国普遍关注和重视，日益成为各国的战略必争装备和竞争核心技术。 应用于危险环境的机器人在作业时往往面对非常复杂的非结构环境。传统机器人存在的不足有：第一：受自身机械结构限制，一般不能够适应多变的地形；第二：大多数都是人为控制的，导致了在对人类有危害的环境中，机器人的行动范围受到很大的限制。本文研究的主要工作是针对可变形机器人的结构特点，提出了一种基于多传感器信息融合的可变形机器人自主控制的方法。该方法主要实现了可变形机器人根据环境变化和任务需要自主变形、自主避障和自主导航定位的功能，增强了可变形机器人的环境适应能力和作业能力。 本文首先对可变形移动机器人的发展和研究现状进行了综述，指出了多传感器信息融合应用在可变形机器人中的发展趋势。然后详细的介绍了可变形机器人的车体和控制系统结构以及机器人最基本的两种运动：直线运动和转弯运动。接着针对可变形机器人三种基本构形的特点，制定了机器人自主变形和避障的准则以及基于GPS/DR惯性导航定位的实现方法，并通过实验验证了机器人在非结构环境中自主变形越障和导航这种控制方法的有效性。 最后，本文对可变形机器人的发展和后继研究工作进行了展望。

基于传感器的模糊参数自整定柔性关节抑制震动的研究

本文以谐波驱动系统为例分析了柔性关节机械手的动力学特性，通过电机端和负载端PD控制结合负载端的加速度反馈控制，并采用模糊参数自整定方法，能有效的抑制负载端的振动，通过几种方法的仿真结果比较表明，提出的方法是有效的。

Distributed Temperature Sensing System Based on Rayleigh Scattering BOTDA

A distributed temperature sensor based on Rayleigh scattering Brillouin optical time domain analysis (Rayleigh-BOTDA) is proposed in this paper. The sensor uses Rayleigh backscattering effect of microwave modulated pulse base sidebands as probe wave and a high sensitive photon counting detector for Brillouin signal intensity detection. Compared with a conventional BOTDA system, the Rayleigh-BOTDA effectively suppresses polarization-induced signal fluctuation resulting in improved signal intensity. The experimental scheme presented is simplified by using a single laser with one-end access. The temperature accuracy of the new sensing system was demonstrated as 1 degrees C on spatial resolution of 3 m.

Immunosensors based on layer-by-layer self-assembled Au colloidal electrode for the electrochemical detection of antigen

Colloidal Au particles have been deposited on the gold electrode through layer-by-layer self-assembly using cysteamine as cross-linkers. Self-assembly of colloidal Au on the gold electrode resulted in ail easier attachment of antibody, larger electrode surface and ideal electrode behavior. The redox reactions of [Fe(CN)(6)]-/[Fe(CN)(6)](3-) on the gold surface were blocked due to antibody immobilization, which were investigated by cyclic voltammetry and impedance spectroscopy. The interaction of antigen with grafted antibody recognition layers was carried out by soaking the modified electrode into a phosphate buffer at pH 7.0 with various concentrations of antigen at 37degreesC for 30 min. Further, an amplification strategy to use biotin conjugated antibody was introduced for improving the sensitivity of impedance measurements. Thus, the sensor based oil this immobilization method exhibits a large linear dynamic range, from 5 - 400 mug/L for detection of Human IgG. The detection limit is about 0.5 mug/L.

A chemiluminescence optical fiber glucose biosensor based on co-immobilizing glucose oxidase and horseradish peroxidase in a sol-gel film

An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase (GOD) and horseradish peroxidase (HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0.2-2 mmol/L and the detection limit was approximately 0.12 mmol/L. The relative standard deviation was 5.3% (n = 6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

Study on sensing properties of tin oxide CO gas sensor with low power consumption

In this paper, the fabrication method of a new type of carbon monoxide gas sensor based on SnOx with low power consumption and its sensing characteristics have been reported. The electric conductance of this type of sensor evolves oscillation form regularly when the sensor is exposed to low level of CO gas. The oscillation amplitude is directly proportional to the concentration of CO gas over a wide range. The effects of relevant factors. such as. humidity, temperature and interference gases on the sensor properties were examined. The sensing oscillation response mechanism was also discussed.

MEDIATORLESS HYDROGEN-PEROXIDE ELECTRODE BASED ON HORSERADISH-PEROXIDASE ENTRAPPED IN POLY(O-PHENYLENEDIAMINE)

A mediatorless H2O2 sensor based on coelectropolymerization of horse radish peroxidase (HRP) and o-phenylenediamine (o-PD) is described. The electrode responds to H2O2 in a few seconds and gives a current density of 73.3 nA 1 mu mol(-1) cm(-2) at -100 mV

基于多传感器的可变形机器人自主控制方法研究

将GPS、电子罗盘、倾角仪、码盘传感器等应用到可变形机器人自主运动控制中.针对可变形机器人自身结构特点,提出了一种基于多传感器信息融合的可变形机器人在野外环境中自主控制的方法.该方法主要实现了在非结构环境中机器人的自主变形、自主避障和自主导航定位等功能.实验验证了该方法的有效性.

基于加速度反馈的柔性关节机械臂接触力控制

针对柔性关节机械臂从自由空间运动控制过渡到约束空间力控制的过程中,存在冲击、震荡甚至不稳定等问题,利用加速度传感器反馈控制,为柔性关节机械臂的接触力控制在较宽的带宽内提供阻尼,克服了利用单纯速度反馈控制带宽窄的局限。对柔性关节机械臂的接触力控制进行建模和基于加速度反馈的控制策略分析,并在柔性关节机械臂上进行了接触力控制的试验研究。结果表明,这种方法有效。