Imaging properties of coherent anti-Stokes Raman scattering microscope

The coherent anti-Stokes Raman scattering (CARS) microscope with the combination of confocal and CARS techniques is a remarkable alternative for imaging chemical or biological specimens that neither fluoresce nor tolerate labelling. CARS is a nonlinear optical process, the imaging properties of CARS microscopy will be very different from the conventional confocal microscope. In this paper, the intensity distribution and the polarization property of the optical field near the focus was calculated. By using the Green function, the precise analytic solution to the wave equation of a Hertzian dipole source was obtained. We found that the intensity distributions vary considerably with the different experimental configurations and the different specimen shapes. So the conventional description of microscope (e.g. the point spread function) will fail to describe the imaging properties of the CARS microscope.

The intensity distributions of collected signals in coherent anti-Stokes Raman scattering microscopy

Coherent anti-Stokes Raman scattering (CARS) microscopy with the combining of confocal and CARS techniques is a remarkable alternative for imaging chemical or biological specimens that neither fluoresce nor tolerate labeling. The CARS is a nonlinear optical process, the imaging properties of CARS microscopy will be very different from the conventional confocal microscopy. In this paper, we calculated the propagation of CARS signals by using the wave equation in medium and the slowly varying envelope approximation (SVEA), and find that the intensity angular distributions vary considerably with the different experimental configurations and the different specimen shapes. So the conventional description of microscopy (e.g.. the point spread function) will fail to descript the imaging properties of CARS microscopy. (c) 2004 Elsevier B.V. All rights reserved.

相干反-斯托克斯喇曼光谱术

相干反斯托克斯喇曼光谱(Coherent Anti-Stokes Raman Spectra,简称CARS)是一种非线性光学混频过程。同时使用两条入射激光束聚焦于样品,输出相当于反-斯托克斯频率光束。量子效率可达1%,散射强度比自发喇曼谱高10~5倍以上,连续CARS谱分辩率为0.01cm~(-1)。这种具有高空间分辩、高抗荧光干扰、高分辩率及高效率等特点的CARS技术,

汽车混流总装线投产排序优化研究

汽车混流总装线上交替装配不同型号的汽车产品,不同型号的汽车在同一装配工位上装配的零部件可能不同,如果不对车型的投产顺序加以优化排序,连续投入一种相同的车型,会使同一个工位的零部件消耗率严重不均衡。为了使各装配工位的零部件消耗率达到均匀化和平准化,本文采用模拟退火优化算法,对车型上线序列进行优化,最后的实例验证表明算法是可行的。