Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.

Traveling wave front for the Fisher equation on an infinite band region

Instead of discussing the existence of a one-dimensional traveling wave front solution which connects two constant steady states, the present work deals with the case connecting a constant and a nonhomogeneous steady state on an infinite band region. The corresponding model is the well-known Fisher equation with variational coefficient and Dirichlet boundary condition. (c) 2006 Elsevier Ltd. All rights reserved.