Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp

A systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp (Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963 TBS positive clones and 2466 HBS positive clones were sampled to PCR amplification, and 1373 TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and 272 HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS dot blot positive clones. Of the 79 known genes, 20 were enzymes or kinases involved in important metabolism of embryonic development. Moreover, specific expressions of partial genes were further confirmed by virtual northern blots. This study is the first step for making a large attempt to study temporal and spatial control of gene expression in the gynogenetic fish embryogenesis.

Temperature Sensitivity Dependence on Cavity Length in p-Type Doped and Undoped 1.3-mu m InAs-GaAs Quantum-Dot Lasers

We have fabricated 1.3-mu m InAs-GaAs quantum-dot (QD) lasers with and without p-type modulation doping and their characteristics have been investigated. We find that introducing p-type doping in active regions can improve the temperature stability of 1.3-mu m InAs-GaAs QD lasers, but it does not, increase the saturation modal gain of the QD lasers. The saturation modal gain obtained from the two types of lasers is identical (17.5 cm(-1)). Moreover, the characteristic temperature increases as cavity length increases for the two types of lasers, and it improves more significantly for the lasers with p-type doping due to their higher gain.

基于可信虚拟平台的数据封装方案

可信计算平台的封装存储功能将数据的加密存储与平台配置结合起来,可提供更为强有力的数据安全服务.然而,平台配置的频繁变动如硬件更替、软件更新及系统补丁等又极大地限制了封装存储功能的使用.针对这个问题,提出了一种基于可信虚拟平台的数据封装存储方案.方案引入了虚拟PCR(vPCR)和安全属性的概念,利用可信平台模块(TPM)将数据与系统安全属性封装起来保护.该方案除能适应平台配置频繁变更的问题外,还能同时保护多个虚拟机系统中数据的安全,不受虚拟机系统配置变化的影响.该方案执行操作简单,实验结果表明与原有方案相比,TPM���负担较小,性能无显著差别.

基于进程行为的异常检测模型

利用系统漏洞实施攻击是目前计算机安全面临的主要威胁.本文提出了一种基于进程行为的异常检测模型.该模型引入了基于向量空间的相似度计算算法和反向进程频率等概念,区分了不同系统调用对定义正常行为的不同作用,提高了正常行为定义的准确性;该模型的检测算法针对入侵造成异常的局部性特点,采用了局部分析算法,降低了误报率.

新近纪南海深层水的增氧与分层

综合南海ODP1148站、1146站和1143站沉积物物性、底栖有孔虫、同位素等资料