Karyotypic diversity in polyploid gibel carp, Carassius auratus gibelio Bloch

Polyploid gibel carp, Carassius auratus gibelio, is an excellent model system for evolutionary genetics owing to its specific genetic background and reproductive modes. Comparative karyotype studies were performed in three cultured clones, one artificially manipulated group, and one mated group between two clones. Both the clones A and P had 156 chromosomes in their karyotypes, with 36 metacentric, 54 submetacentric, 36 subtelocentric, 24 acrocentric, and six small chromosomes. The karyotype of clone D contained 162 chromosomes, with 42 metacentric, 54 submetacentric, 36 subtelocentric, 24 acrocentric, and six small chromosomes. All the three clones had six small chromosomes in common. Group G, being originated from the clone D by artificial manipulation, showed supernumerary microchromosomes or chromosomal fragments, in addition to the normal chromosome complement that was identical to the clone D. The offspring from mating between clones D and A had 159 chromosomes. Comparing with the clone A, the DA offspring showed three extra metacentric chromosomes. In addition, variable RAPD fingerprint patterns and unusual SCAR marker inheritance were, respectively, detected among individuals of artificial group G and in the mated DA offspring. Both the chromosome and molecular findings suggest that genome reshuffling might have occurred by manipulation or mating of the clones.

基于汽车检测线的实时网络监控系统

在介绍汽车检测线系统的基础上,探讨了实时网络监控在汽车检测线上的应用,为了克服检测过程存在较多人为因素的缺点和解决汽车检测场社会化引起的监管问题,提出了通过控制摄像头多角度拍摄监控照片对被检车辆进行身份认证的新方法,该方法可以方便异地监控端实时了解汽车检测线上的各类检测情况,并且有助于保证检测过程的真实有效性

Genetic characterization of asymmetric reciprocal hybridization between the flatfishes Paralichthys olivaceus and Paralichthys dentatus

Interspecific reciprocal crosses between the two flatfishes Paralichthys olivaceus and P. dentatus yielded hybrids with different viabilities. Specifically, the hybrids of P. olivaceus female and P. dentatus male (HI) were found to be viable, while the reciprocal hybrids from P. dentatus female and P. olivaceus male (HII) were completely inviable. All the HII individuals showed morphological deformities and died before first feeding. The chromosome analysis showed that HI individuals had the same chromosome number as parents. However, two chromosomes were missing in HII offspring indicating that the latter were aneuploids. Genomic inheritance from the parents to F-1 progeny was also examined by amplified fragment length polymorphism (AFLP) analyses, and the results showed differences between reciprocal hybrids. Almost all AFLP bands (97.71%) observed in parents were passed on to HI individuals. In contrast, only 86.64% of the AFLP bands from parents were scored in HII individuals. Frequency of lost parental bands was thus significantly higher in HII than that in HI and intraspecific crosses, which was probably associated with chromosomal elimination. In addition, higher segregation distortions were found in hybrids than in controls, although these differences were not significant. The present study indicates that chromosomal elimination and loss of AFLP loci occurred in inviable HII individuals, while such genomic changes were not found in viable HI individuals. Possible implications of such difference on genomic changes for asymmetric viability in reciprocal hybrids are discussed.

Microsatellite-centromere distances and microsatellite diversity in different ploidy classes of Chinese shrimp (Fenneropenaeus Chinensis)

This is the first report of microsatellite-centromere mapping in this commercial species Fenneropenaeus Chinensis, and will be important for providing fixed points in the linkage groups of genetic maps. Triploid Chinese shrimp was induced by heat shock. The fertilized eggs were treated either by retention of the first polar body or the second polar body to produce Meiosis I (MI) or Meiosis II (MII) triploid. The triploidy status in each Chinese shrimp could be confirmed by nine polymorphic microsatellite loci, in which the parents with different alleles and the female parents were each heterozygous. The nine loci were mapped in relation to their centromeres in three MII triploid families, which were induced by retention of the second polar bodies after fertilization with sperm. Microsatellite-centromere (M-C) distances ranged from 9.6 cM to 37 cM under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards assembly of genetic maps in F. chinensis. Twelve polymorphic microsatellites were used to assess the heterozygosity and allelic diversity in different ploidy classes. As expected, triploids were significantly more polymorphic than diploids. The diploids had an average heterozygosity and allelic diversity value of 0.86, whereas the triploids heterozygosity averaged 0.93 and had allelic diversity value of 1.29. However, MI triploids were not significantly more polymorphic than MII in the microsatellite loci.

A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates

Pacific white shrimp (Litopenaeus vannamei) is the leading species farmed in the Western Hemisphere and an economically important aquaculture species in China. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP) and microsatellite markers. One hundred and eight select AFLP primer combinations and 30 polymorphic microsatellite markers produced 2071 markers that were polymorphic in either of the parents and segregated in the progeny. Of these segregating markers, 319 were mapped to 45 linkage groups of the female framework map, covering a total of 4134.4 cM; and 267 markers were assigned to 45 linkage groups of the male map, covering a total of 3220.9 cM. High recombination rates were found in both parental maps. A sex-linked microsatellite marker was mapped on the female map with 6.6 cM to sex and a LOD of 17.8, two other microsatellite markers were also linked with both 8.6 cM to sex and LOD score of 14.3 and 16.4. The genetic maps presented here will serve as a basis for the construction of a high-resolution genetic map, quantitative trait loci (QTLs) detection, marker-assisted selection (MAS) and comparative genome mapping.

Comparative molecular population genetics of phycoerythrin locus in Prochlorococcus

As the only remainder type of phycobiliproteins in Prochlorococcus, the actual role of phycoerythrin still remains unknown. Previous studies revealed that two different forms of phycoerythrin gene were found in two ecotypes of Prochlorococcus that are specifically adapted to either high light (HL) or low light (LL) conditions. Here we analyze patterns of phycoerythrin nucleotide variation in the HL- and LL-Prochlorococcus populations. Our analyses reveal a significantly greater number of non-synonymous fixed substitutions in peB and peA than expected based on interspecific comparisons. This pattern of excess non-synonymous fixed substitutions is not seen in other five phycoerythrin-related genes (peZ/V/Y/T/S). Several neutrality statistical tests indicate an excess of rare frequency polymorphisms in the LL-Prochlorococcus data, but an excess of intermediate frequency polymorphisms in the HL-Prochlorococcus data. Distributions of the positively selected sites identified using the likelihood ratio test, when mapped onto the phycoerythrin tertiary structure, reveal that HL- and LL-phycoerythrin should be under different selective patterns. These findings may provide insights into the likely role of selection at the phycoerythrin locus and motivate further research to unveil the function of phycoerythrin in Prochlorococcus.

Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau

We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of H-t and H-sp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, H-e and H-pop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei's coefficient of differentiation (G(ST)) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species.

质子交换膜燃料电池在无缆水下机器人上的应用研究

质子交换膜燃料电池(PEMFC)作为一种新兴的能源,在汽车和航天器上已经获得成功的应用,本文针对水下环境和PEMFC的特点,对PEMFC在无缆水下机器人(UUV)上试验应用的关键技术进行讨论.

一种柔性冲压自动化生产线系统的研制

本文介绍了一种用于大型汽车覆盖件冲压生产的柔性自动化生产线，系统由６台压机、１２台上下料机械手、１台翻转传输装置、４台穿梭传输装置及１台磁力分层装置构成．给出了生产线的主要性能指标、机械手结构简图及单元控制系统框图，简要介绍了系统的工作原理。

变速箱试验环线集成控制系统

研究当前国内外变速箱试验系统的特点,基于现代汽车变速箱生产信息化、网络化和柔性化的要求,依托某变速箱装配线项目,针对其试验环线,提出一种具有并行结构的变速箱自动试验环线,并设计基于Device Net的控制系统,探究试验环线的智能控制。该系统已经进入实际应用,可较好地完成变速箱的性能检测以及试验后的数据分析。

基于数字化生产模型的在线故障诊断技术研究

提出了一种基于数字化的生产模型,使用控制图、故障树分析和专家知识,能够进行制造过程实时监控的诊断,该模型提高了故障诊断系统的可靠性,并提供了可实际操作的可视化建模工具。所开发的在线统计过程控制系统能够根据生产事件的监测,动态响应制造过程变化。该系统运用可视化建模工具,根据专家经验进行故障树建模,通过故障树自动生成专家系统诊断规则库,实现诊断知识的自动获取。将该系统应用于汽车变速箱装配过程的检测与故障诊断,验证了方法的有效性。

一种基于多特征融合的汽车变速箱螺栓在线视觉检测系统

本文根据汽车变速箱装配线螺栓检测的要求,结合检测现场的实际图像特点,采用融合灰度投影与颜色直方图两种特征的方法,利用相似性测度的计算方法,设计了一种变速箱螺栓在线装配质量检测系统,该系统用于实现螺栓的的缺失及漏拧检测,试验结果表明,方法具有良好的实用性,取得了比较理想的效果。

基于RFID的混流装配汽车生产线物料动态配送研究

针对混流装配的汽车自动化生产线提出了基于RFID的车辆追踪和基于条码的无线物料库存管理实现方法。在此基础上,设计了装配生产线的物料动态配送调度业务流程,给出了物料动态配送的规划依据,并进行了实例计算。

汽车变速箱性能检测系统设计与实现

本文从整体上论述了汽车变速箱性能检测系统的测试原理和设计方案,并从硬件和软件两方面详细阐述了汽车变速箱性能检测系统的组成。现场总线的采用,使本系统的结构更加简单,实施更加方便。实践证明,该方法是可行的,可靠的。

面向汽车变速器状态监测的虚拟仪器系统研究

摘要 变速器是汽车主要部件之一，其质量直接影响整车的性能和质量。对装配后的变速器进行下线检测是生产过程必需的步骤。目前我国汽车变速器的下线检测多数在变速器试验台上进行，由工人耳听变速器的振动噪声判断其是否合格。人工判断的方法存在精度低、随机性高和受工人经验、情绪影响大等缺点。因此，实现变速器状态监测的自动化，研究一种适用于汽车变速器下线检测的状态监测系统具有很大的现实意义。 虚拟仪器技术构建方式快速灵活、人机界面友好、具有强大的信号处理能力，而且可以充分利用变速器试验台已有的工控机平台。本论文研究开发了一套面向汽车变速器状态监测的虚拟仪器系统。 第一章介绍了课题来源和研究目的；综述了虚拟仪器的发展和变速器状态监测与故障诊断的研究现状。 第二章分析了面向振动信号的常用信号处理方法及其不足、介绍了时域同步平均和基于重采样的阶次分析方法，典型齿轮故障的时频域特征。 第三章根据变速器状态监测的要求设计并实现了状态监测的硬件系统，主要包括传感器、数据采集卡。 第四章开发了基于虚拟仪器的软件系统，完成了参数设置模块、数据采集模块、信号预处理模块、时域分析模块、频域分析模块、时域同步平均模块、阶次分析模块、文件管理模块和网络通信模块的具体设计，并将这些模块在主程序的程序框图中按照一定的逻辑关系组合起来，形成一个完整的变速器状态监测系统。 第五章对本文工作做了总结并对以后工作进行了展望