Dual Confocal Laser-Induced Fluorescence/Moveable Contactless Conductivity Detector For Capillary Electrophoresis Microchip

A new dual simultaneous detector was developed for capillary electrophoresis microchip. Confocal laser-induced fluorescence (LIF) and moveable contactless conductivity detection (MCCD) were combined together for the first time. The two detection systems shared a common detection cell and could respond simultaneously. They were mutually independent and advantageous in analyses of mixtures containing organic and inorganic ions. The confocal LIF had high sensitivity and the MCCD could move along the separation channel and detect in different positions of the channel. The detection conditions of the dual detector were optimized. Rhodamine B was used to evaluate the performance of the dual detector. The limit of detection of the confocal LIF was < 5 nM, and that of the MCCD was 0.1 mu M. The dual detector had highly sensitivity and could offer response easily, rapidly and simultaneously. 

一种基于PSD器件的改进型角度测量方法

提出一种基于平行平板干涉仪的改进型角度测量方法。为了实现较大的偏转角度测量，该平行平板干涉仪引入了一位置探测系统。平面反射镜的引入提高了角度测量的分辨率。实验验证了可在近3度的范围内实现被测偏转角度的高精度测量。并且作为一位相调制型干涉仪，其小角位移测量实验的重复精度可达5.5×10^(-8)rad。

高功率激光装置光束准直系统新型远场监测技术

利用高功率激光装置空间滤波器小孔成像和取样光栅的衍射,设计出一套新型光路远场监测方案,并且在实验平台上进行了实验验证.实验结果表明:相对传统的远场监测方法,该远场监测系统通过侧面离轴光栅取样灵活利用空间,其调整平均误差为空间滤波器小孔直径0.9%,能够满足准直系统远场调整精度(<小孔直径5%)的要求.

上转换发光免疫试纸条扫描检测系统研究

研制了一种以上转换发光材料作为标记物的扫描检测系统，有别于传统的测量方法，可做出定量和多重分析．扫描检测系统以扫描方式逐点测量经生物反应而结合上去的UCP颗粒的含量，记录整个免疫试纸条的信息，计算出被测样品中特定生物分子的浓度．实验结果表明，该系统对兔抗鼠疫免疫球蛋白（IgG）标准样品的检测灵敏度达到百ng／mL量级，且具有变异系数小于6％的重复性，符合生物免疫测定的要求．

基于一维CCD的免疫层析试纸条检测系统

本文报道的基于一维CCD的免疫层析试纸条检测系统是一种以上转换磷光材料（UCP）作为标记物的生物传感器。该系统通过检测生物反应后试纸条上UCP颗粒的含量，计算出被测样品中特定生物分子的浓度，可以实现对多种病原体的快速定性与定量检测。本检测系统对0—60ns／ml系列标准样品的检测结果具有很好的线性响应特性，且与扫描型检测系统的检测结果十分接近。

Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone (cGH) in common carps (Cyprinus carpio)

The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions.

一种基于移动代理自动优化的分布式入侵检测系统

随着网络技术的飞速发展，网络入侵检测系统需要处理大量的数据，处理能力的缺乏会引起入侵事件的漏报或误报，提高入侵检测系统的处理能力是目前急需解决的关键问题．AODIDS是一个由移动代理作为优化组件、多个分析结点及探测结点组成的可自动进行优化的分布式网络入侵检测系统．AODIDS的优化组件执行系统的性能评估，制定相应的优化策略，在规定的系统检测正确率的前提下平衡分配网络流量，从而尽可能地发挥整个系统的处理能力．

一种基于Agent的自适应的分布式入侵检测系统

针对传统分布式入侵检测系统组件之间依赖程度大、系统不够健壮且入侵检测系统自身结构固定不能适应入侵的变化的问题,提出了一种基于Agent的自适应的分布式入侵检测系统(简称AAA-DIDS)·AAADIDS采用Agent概念重新构造系统的组件,改进了分布式入侵检测系统由于高层节点单一无冗余而产生的可靠性差的缺陷,从构造上克服了分布式入侵检测系统的脆弱性·同时,AAADIDS系统采用智能技术构建了自适应的入侵检测系统模型,增加了系统应对入侵行为变化的智能性·AAA-DIDS系统相对于传统的分布式入侵检测系统有效地提高了系统自身的可靠性和针对外界变化的适应能力·

A New Method to Investigate InGaAsP Single-Photon Avalanche Diodes Using a Digital Sampling Oscilloscope

A near-infrared single-photon detection system is established by using pigtailed InGaAs/InP avalanche photodiodes. With a 50GHz digital sampling oscilloscope, the function and process of gated-mode (Geiger-mode) single-photon detection are intuitionally demonstrated for the first time. The performance of the detector as a gated-mode single-photon counter at wavelengths of 1310 and 1550nm is investigated. At the operation temperature of 203K,a quantum efficiency of 52% with a dark count probability per gate of 2. 4 * 10~(-3), and a gate pulse repetition rate of 50kHz are obtained at 1550nm. The corresponding parameters are 43% , 8. 5 * 10~(-3), and 200kHz at 238K.

Non-reproducibility of the cross sections of the products induced from dissipative reaction of F-19+Al-27

The differential cross sections of the dissipative products B, Q N, O, F, Ne, Na and Mg induced from the reactions of F-19+Al-27 at two incident energies have been measured at the HI-13 tandem accelerator, Beijing. In the case of a fixed beam incident energy 114MeV or 118.75MeV respectively, identical reaction system and the same detection system, 20 target points in steps of 2mm on(.)a 10mmx50mm rectangular Al foil have been bombarded. The experimental results indicate that the probability distribution of the cross sections is much wider than a standard Gaussian distribution. This non-reproducibility of the cross sections can't be interpreted by the statistical property of a finite count rate.

Kicker电源数字延迟线及低压监测系统设计

踢轨磁铁（Kicker）电源系统是HIRFL-CSR注入引出系统中实现快引出的一个关键元件，主要功能是为踢轨磁铁提供快脉冲励磁电流以产生所需要的快脉冲磁场。Kicker电源提供的是高电压大电流的快脉冲，电流脉冲上升沿和下降沿为150ns,脉冲宽度为650ns,其脉冲峰值电流为2700A，工作周期为10s-17s。因此及时监控Kicker电源闸流管的工作状况以及电流脉冲波形特性至关重要。本文针对踢轨磁铁（Kicker）电源的需要，进行了Kicker电源监测系统的设计，主要针对闸流管误漏导通检测、电流脉冲宽度过宽过窄检测、脉冲宽度测量及脉冲计数等功能提出了电路的工作原理，并设计了具体电路。系统输入端采用光纤接口，而输出端采用了PLC数字I/O接口。由于采用PLC接收监测电路板的信号来完成对Kicker电源的监控报警，基于此编写了相关PLC程序，并调试通过。该监测系统电路板已调试完成，可以很好地完成对Kicker电源系统较为全面的状态监测，方便地对Kicker电源系统状态进行监控。另外，为了解决Kicker电源系统脉冲同步的问题，以满足兰州重离子加速器冷却储存环（HIRFL-CSR）环踢轨磁铁（Kicker）电源对电流脉冲进行适当延迟的要求，还分别设计了ECL高速可程控数字延迟线电路系统和基于CPLD的数字延迟线系统，分析介绍了数字延迟线系统结构、工作原理及PCB版图设计等。ECL高速可程控数字延迟线电路已初步调试通过，而基于CPLD的数字延迟线系统已完成了程序编程及仿真工作，它克服了ECL数字延迟线不能实现零延迟的缺点，且可以通过修改VHDL程序来设置出更多位的可编程数字延迟线，方便灵活

小型符合影像探测系统的研制及其应用研究

随着中科院近代物理研究所重离子束治癌技术和浅层治疗应用基础研究的不断开展，对束流入射深度分布的影像测试研究成为了重要的研究课题。本论文基于此背景，成功研制了小型符合影像探测系统，并进行了深入的影像测试应用研究。符合影像探测的基本原理为通过符合探测系统对正电子放射性核素引起的正负电子湮灭辐射进行符合测量，并利用相应的影像重建方法就可以得到影像体的影像结果。研制的符合影像探测系统由两个探测单元组成，单元的核心探测器件为具有64个通道的位置灵敏平板型光电倍增管H8500，其前端与性能优良的BGO闪烁晶体阵列耦合，输出信号采用电阻网络简化读出。通过比较蒙特卡罗模拟结果和实际测试结果，优化设计了符合探测单元，主要确定了闪烁晶体单元的大小和组成阵列的构形：探测阵列由19X19块晶体单元组成，每块晶体单元大小为2X2X10mm3，间距为0.3mm的Tefon薄膜隔离。基于这种单元构形，探测单元的本征位置分辨约为：1.37mm(FWHM)，组成的符合影像探测系统空间位置分辨约为（对22Na源）：1.87mm(FWHM)。上述指标接近于小型符合探测系统最佳本征和空间位置分辨。符合影像探测系统分别在22Na 正电子发射源和12C束流入射有机靶不同条件下进行测试研究。采用中心焦平面影像原理进行数据的影像重建，得到了符合探测系统的空间位置分辨能力、最大计数率和靶内深度分布的影像测试结果。分析束流入射PMMA靶60秒后的测量数据，成功分离了靶内10C、11C 和15O 三种主要正电子放射性核素产物，对不同产物的深度分布与测试布拉格峰位进行了比较，得到了一种如何实现浅层治疗在线监测的研究方法。实验研究结果表明，研制的这一套2D符合影像探测系统具有高的空间位置分辨率、较大的探测视野(约50X50mm3)和较好的影像能力。12C束流入射有机靶的影像测试研究结果表明，该方法和该探测系统可以应用于重离子浅层治疗，可以在治疗过程中实现快速地、准确地对布拉格峰位进行监测

Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe

A flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (ACNE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 mug/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed. (C) 2004 Elsevier B.V. All rights reserved.

Reducing H2S production by O-2 feedback control during large-scale sewage sludge composting

Hydrogen sulfide (H2S) production patterns and the influence of oxygen (O-2) concentration were studied based on a well operated composting plant. A real-time, online multi-gas detection system was applied to monitor the concentrations of H2S and O-2 in the pile during composting. The results indicate that H2S was mainly produced during the early stage of composting, especially during the first 40 h. Lack of available O-2 was the main reason for H2S production. Maintaining the O-2 concentration higher than 14% in the pile could reduce H2S production. This study suggests that shortening the interval between aeration or aerating continuously to maintain a high O-2 concentration in the pile was an effective strategy for restraining H2S production in sewage sludge composting. (C) 2010 Elsevier Ltd. All rights reserved.