A new anti-HIV lupane acid from Gleditsia sinensis Lam.

A new lupane acid, 2 beta-carboxyl, 3 beta-hydroxyl-norlupA (1)-20 (29)-en-28-oic acid (1), together with five known lupane acid derivatives (2-6), were isolated from the stings of Gleditsia sinensis Lam.. Their structures were elucidated on the basis of

β2受体遗传多态性与支气管哮喘患者肺功能关系研究

β_2肾上腺素受体（β_2AR）在哮喘患者中表现出遗传多态性。近年国外已对位点16及27进行了一些临床研究，但是所用基因分型方法不一，此外在中国人群这2个位点的多态性哮喘的关系至今仅有个别报道，我们采用所有样本测序方法，就其多态性与肺功能的关系进行初步探讨。

Anti HIV-1 agents 5: Synthesis and anti-HIV-1 activity of some N-arylsulfonyl-3-acetylindoles in vitro

In continuation of our program aimed at the discovery and development of compounds with superior anti-human immunodeficiency virus type 1 (HIV-1) activity, 21N-arylsulfonyl-3-acetylindole analogs (2a-u) were synthesized and preliminarily evaluated as HIV-1 inhibitors in vitro. Among of all the analogs, several compounds exhibited significant anti-HIV-1 activity, especially N-phenylsulfonyl-3-acetyl-6-methylindole (2j) and N-(p-ethyl)phenylsulfonyl-3-acetyl-6-methylindole (2n) showed the most potent anti-HIV-1 activity with EC50 values of 0.36 and 0.13 mu g/mL, and TI values of >555.55 and 791.85, respectively. It demonstrated that introduction of the acetyl group at the 3-position of N-arylsulfonyl-6-methylindoles could generally lead to the more potent analogs. (C) 2010 Elsevier Ltd. All rights reserved.

石榴干腐病生物生态学及发生流行规律和治理研究

云南蒙自石榴干腐病助故阮以菌丝体和分生抢子在僵果、干 腐果及其他感病部位越冬。翌年经风雨、昆虫和人为活动在果园广为传播。石榴 花、幼果、果实期均可感病发病, 等筒花丝最易感病。果实发病率达一, 重者达一。采取综合治理措施, 其中重要的是在首次连续降雨前利去干 花丝、套袋和树冠多次喷清水, 提前水解、淡化干花丝、伤口渗出液的微酸性度, 可 控制该病的发生危害, 危害率由原来的一现降为一。

家鸡住肉孢子虫调查及实验感染研究

作者于１９８９年３月到１９９０年３月对昆明市２８４只家鸡进行了住肉孢子虫流行病学调查，在６只家鸡中检到了住肉孢子虫包囊，自然感染率为２．１％。包囊呈梭形，大小为８６５．５×８２．３μｍ，包囊中充满大小为１４．３×２．９μｍ的梭形缓殖子。鸡颈部、背部和大腿部肌肉中包囊密度较高，心肌无包囊寄生。４只犬和５只猫分别于实验感染包囊后第８ｄ和第１３ｄ排出孢子，剖检时小肠粘膜固有层中亦查到孢子。１０只１５日龄的雏鸡在实验感染来源于犬和猫粪便的孢子后，干不同时间剖杀镜检，除感染后第２０ｄ剖杀的２只鸡外，其余８只鸡肌肉中均检到包囊，其形态与自然感染鸡体的包囊相同。实验感染结果表明犬和猫均可充当该住肉孢子虫的终末寄主。虫种鉴定为Ｓａｒｃｏｃｙｓｔｉｓｓｐ。是国内家鸡住肉孢子虫的首次报告。

微囊藻衰亡过程研究——四种模拟胁迫条件下微囊藻的衰亡生理

研究了四种胁迫条件下微囊藻衰亡过程以及衰亡过程中生长生理与抗氧化酶系统的变化以及胁迫30d后转入正常培养后的微囊藻的生长变化过程.将对数生长期的微囊藻分别转入氮饥饿、磷饥饿、黑暗及10℃低温等胁迫条件下培养,测定光密度OD680、叶绿素a、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性等生理指标.结果表明,在黑暗和低温条件下,微囊藻生长受到明显抑制,但黑暗条件下25d后微囊藻衰亡,低温条件下仅需7d;与物理因子引起的胁迫相比,在营养胁迫条件下,微囊藻在胁迫前期均表现出生长趋势.N饥饿条件下叶绿素a含量

胭脂鱼弹状病毒对鲤科鱼类感染性的测定

中国科学院生命科学与技术特别支持费 (STZ 0 0 1 3 ) ; 中国科学院创新项目 (2 2 0 3 1 3 ) ; 淡水生态与生物 技术国家重点实验室基金 (1 999FB1 2 )资助

草鱼、中华鳖脾细胞培养上清液IL-2物质的检测

用刀豆蛋白A(ConA)刺激诱导草鱼和中华鳖脾细胞 ,收集细胞培养上清液 ,用小鼠胸腺细胞增殖试验和对小鼠L92 9细胞系杀伤试验检测上清液中白细胞介素 2 (简称IL 2 )活性。结果表明 :草鱼、中华鳖脾细胞培养上清液中有IL 2样活性物质 ,这种物质使小鼠的胸腺细胞3H TdR掺入量明显增加 ,在相同效靶比的条件下对小鼠L92 9细胞系杀伤率也显著增强 ,这种IL 2活性均能被抗人rIL 2血清所抑制。中华鳖脾细胞培养上清液 (含IL 2 )对中华鳖胸腺细胞也有较明显的促增殖作用并能消除兔抗中华鳖胸

草鱼、中华鳖IL-2活性影响因素的研究

结果显示 :当年草鱼种和中华鳖脾细胞培养上清液中能检测出IL 2活性 ,而且 1龄以上草鱼、中华鳖的IL 2活性高于当年孵化的草鱼和中华鳖。草鱼、中华鳖脾细胞在 2 5℃培养温度条件下 ,其上清液中IL 2活性最高 ,35℃次之 ,1 5℃最低。因此 ,草鱼、中华鳖IL 2活性在一定范围内是随着年龄增加而增强和依赖温度的。通过小鼠胸腺细胞增殖和对小鼠L92 9细胞杀伤率的实验表明 37℃比 2 5℃检测温度下所测的IL 2活性高 ,而中华鳖胸腺细胞增殖实验却显示 2 5℃检测温度下IL 2活性高于 37℃

武汉东湖的轮虫

本文根据1973—1975、1979—1982年在武汉东湖进行的调查资料,并与1962—1963年他人的研究结果对比,总结了20年来,在东湖2个采样站上轮虫的种类演替和数量的变动。轮虫的种类明显地减少。Ⅰ站由1962—1963年的68种至1982年减为44种;Ⅱ站由56种减为49种。由原来Ⅰ站种类多于Ⅱ站演变为Ⅱ站多于Ⅰ站。轮虫的数量急剧增加。Ⅰ站轮虫的年平均值由1962—1963年的687个/升至1982年上升为3,555个/升;Ⅱ站由332个/升上升为1,538个/升。轮虫总数的变动曲线呈夏季和秋季二

Development of novel EST-SSR markers in common carp by data mining from public EST sequences

Expressed sequence tags (ESTs) are a source for microsatellite development. In the present study, EST-derived microsatelltes (EST-SSRs) were generated and characterized in the common carp (Cyprinus carpio) by data mining from updated public EST databases and by subsequent testing for polymorphism. About 5.5% (555) of 10,088 ESTs contain repeat motifs of various types and lengths with CA being the most abundant dinucleotide one. Out of the 60 EST-SSRs for which PCR primers were designed, 25 loci showed polymorphism in a common carp population with the alleles per locus ranging from 3 to 17 (mean 7). The observed (H-O) and expected (HE) heterozygosities of these EST-SSRs were 0.13-1.00 and 0.12-0.91, respectively. Six EST-SSR loci significantly deviated from the Hardy-Weinberg equilibrium (HWE) expectation, and the remaining 19 loci were in HWE. Of the 60 primer sets, the rates of polymorphic EST-SSRs were 42% in common carp, 17% in crucian carp (Carassius auratus), and 5% in silver carp (Hypophthalmichthys molitrix), respectively. These new EST-SSR markers would provide sufficient polymorphism for population genetic studies and genome mapping of the common carp and its closely related fishes. (c) 2007 Published by Elsevier B.V.

Distribution of the meiofaunal community in a eutrophic shallow lake of China

In a Chinese eutrophic shallow lake, the spatial, temporal and vertical distributions of meiofauna in different lake zones along a eutrophic gradient were analyzed. The spatial distribution of meiofauna among sampling stations changed with nutrient levels. Nematoda were most abundant at the majority of sampling stations comprising 70.6 - 93.2 % meiofaunal abundance except for a hypereutrophic station. The seasonal patterns in abundance of nematodes, oligochaetes, rotifers, chironomids and different nematode feeding groups differed among stations, which revealed that the temporal variations of these meiofaunal groups and the nematode feeding groups may vary with different nutrient loadings. The vertical distributions of meiofaunal groups, nematode species, and nematode trophic groups in the upper and lower sediment layers were similar, suggesting a consistent vertical distribution pattern across different trophic conditions. Nematode species richness, Shannon-Wiener species diversity index, trophic diversity and Maturity Index were significantly correlated with nutrient levels (total phosphorus and nitrogen in lake water and total phosphorus in sediment). Our results suggest the importance of nematode community analyses in the assessment of freshwater eutrophication.