裸子植物次生韧皮部的比较解剖学研究

本研究在光镜和扫描电子显微镜下对裸子植物10个科、38属、53种裸子植物茎或枝的次生韧皮部解剖结构进行观察和描述。裸子植物次生韧皮部包括轴向系统（由筛胞、Strasburger细胞、韧皮薄壁组织细胞、纤维、石细胞、轴向树脂道等组成）和径向系统（由射线薄壁组织细胞、径向树脂道等组成），并具有特征性的排列样式。裸子植物各类群的次生韧皮部的组成和排列差异较大，可根据其特征进行此类植物的分类鉴定和和系统关系的探讨。本文还提出了一个根据次生韧皮部解剖结构特征的裸子植物属检索表。

No accelerated evolution of 3 ' UTR region in human for brain-expressed genes

The difference in cognitive skills between humans and nonhuman primates is one of the major characters that define our own species. It was previously hypothesized that this divergence might be attributable to genetic differences at gene expression level,

家养双峰驼线粒体DNA遗传多样性的研究

在双峰驼mtDNA中未发现长度变异和个体内异质现象。从3个类群的87个个体中，共检出了35种限制性态型，12种mtDNA单倍型，家驼群内核苷酸歧异度（#pi#）为0.2273%，这表明我国的家驼在mtDNA水平存在较丰富的遗传多样性；单倍型分布的差异显著性检验及单倍型的共享度分析表明，mtDNA在这3个类群之间没有明显的遗传分化，其较丰富的遗传变异主要来自群体内的差异，与类群间的遗传分化关系不大。

Secondary structural analysis of the mRNA regions encoding the hemagglutinin cleavage site basic amino acids of the avian influenza virus H5N1 subtype samples

Here we report the codon bias and the mRNA secondary structural features of the hemagglutinin (HA) cleavage site basic amino acid regions of avian influenza virus H5N1 subtypes. We have developed a dynamic extended folding strategy to predict RNA secondar

Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry

Many fluorescent probes excited by visible light have been used to assess sperm quality by flow cytometry. Developing a viability evaluation method using UV excited stains would be useful for multiparameter analysis of sperm function. This investigation was conducted to determine the efficacy of Hoechst 33342 (H342) and propidium iodide (PI) dual staining for evaluating rhesus monkey sperm viability through use of flow cytometry and excited by a single UV laser. The results showed that the live cells stained only with H342 strongly correlated with expected sperm viability, and flow cytometric analyses were highly correlated with fluorescence microscopic observation. Using H342/PI/SYBR-14 triple staining method, it was found that the live/dead sperm distributions were completely concordant in both H342/PI and SYBR-14/PI assays. In addition, this dual staining was extended with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) to simultaneously analyze viability and acrosome integrity of sperm cryopreserved using two different extenders, TTE and TEST, and indicated that TTE offered better Preservation of plasma and acrosome integrity than TEST Therefore, the H342/PI dual staining provides an accurate technique for evaluating viability of rhesus monkey sperm and should be valuable for multiparameter flow cytometric analysis of sperm function.