一氧化氮对人肝癌细胞SMMC-7721辐射敏感性的增强效应

目的:观察一氧化氮对肿瘤细胞SMMC-7721辐射敏感性的作用效果。方法:实验于2005-06/09在兰州大学生命科学学院和中科院近代物理研究辐射医学实验室进行。处于对数生长期的肝癌细胞SMMC-7721,在用X射线照射前4h,换入含有0.1mmol/L硝普钠(一氧化氮的前体)的培养液,与对照组(不加硝普钠)一起,在200cGy/min的剂量率下,分别照射0,1,2,4,6,8Gy,换为正常培养液培养。用集落形成法计算细胞的存活率,用吖啶橙/溴乙啶双染法检测细胞的死亡情况,用流式细胞仪检测细胞周期。结果:①存活曲线细胞存活率随照射剂量增加而减少,硝普钠组细胞的克隆形成率低于对照组(2Gy时,P<0.01)。②细胞死亡百分率(坏死细胞与凋亡细胞总数/总细胞数):与照射剂量呈正相关,硝普钠组高于对照组(P<0.05)。对照组从(9.95±3.53)%(0Gy)逐渐升至(58.74±3.46)%(6Gy),而硝普钠组则从(18.53±12.02)%(0Gy)迅速升至(61.57±9.53)%(2Gy)。③细胞周期检测结果:对照组细胞经过X射线照射后,出现了G2/M期阻滞[从0Gy时(12.50±5.76)%逐渐增加到8Gy(40.36±2.74)%],而硝普钠组细胞在低剂量时主要表现为G0/G1期阻滞[0Gy:(16.06±7.19)%;2Gy:(17.93±0.92)%],而G2/M期阻滞仅在高剂量时明显[8Gy时为(50.10±3.93)%,P<0.05]。结论:经硝普钠产生的一氧化氮,通过与X射线协同作用,减少了肝癌细胞SMMC-7721的细胞存活率,促进细胞死亡,阻止细胞被阻滞至G2/M期,是一种有效的辐射增敏剂。

A strategy for synthesis of ion-bonded supramolecular star polymers by reversible addition-fragmentation chain transfer (RAFT) polymerization

We have developed a novel strategy for the preparation of ion-bonded supramolecular star polymers by RAFT polymerization. An ion-bonded star supramolecule with six functional groups was prepared from a triphenylene derivative containing tertiary amino groups and trithiocarbonate carboxylic acid, and used as the RAFT agent in polymerizations of tert-butyl acrylate (tBA) and styrene (St). Molecular weights and structures of the polymers were characterized by H-1 NMR and GPC. The results show that the polymerization possesses the character of living free-radical polymerization and the ion-bonded supramolecular star polymers PSt, PtBA, and PSt-b-PtBA, with six well-defined arms, were successfully synthesized.

Turn-on fluorescent detection of cyanide based on the inner filter effect of silver nanoparticles

A simple, sensitive fluorescent method for detecting cyanide has been developed based on the inner filter effect (IFE) of silver nanoparticles (Ag NPs). With a high extinction coefficient and tunable plasmon absorption feature, Ag NPs are expected to be a powerful absorber to tune the emission of the fluorophore in the IFE-based fluorescent assays. In the present work, we developed a turn-on fluorescent assay for cyanide based on the strong absorption of Ag NPs to both excitation and emission light of an isolated fluorescence indicator. In the presence of cyanide, the absorber Ag NPs will dissolve gradually, which then leads to recovery of the IFE-decreased emission of the fluorophore. The concentration of Ag NPs in the detection system was found to affect the fluorescence response toward cyanide greatly. Under the optimum conditions, the present IFE-based approach can detect cyanide ranging from 5.0 x 10 (7) to 6.0 x 10 (4) M with a detection limit of 2.5 x 10 (7) M, which is much lower than the corresponding absorbance-based approach and compares favorably with other reported fluorescent methods.

两种钌(Ⅱ)络合物的核磁共振氢谱分析

报道了顺－二氯二联吡啶钌（Ⅱ）和顺－二氯二邻二氮菲钉（Ⅱ）的核磁共振氢谱。利用１Ｈ－１Ｈ ＣＯＳＹ谱详细分析并归属了这两种络合物的氢谱峰，并给出了各质子的偶合数，同时验证了其顺式的立体构型。

Gas phase derivations of endohedral metallofullerenes by ion-molecular reactions

Gas phase ion-molecular reactions of endohedral metallofullerenes with the self-chemical ionization ion system of vinyl acetate, benzene and acetone in the ion source of the mass spectrometer have been studied. Several derivatized endohedral metallofullerene cations [M@C-82-C2H3O](+), [M-2@C-80-C2H3O](+), [M@C-82-C6H6](+) and [M@C-82-CO-CH3](+) are observed as the major products. The experimental results indicate that endohedral metallofullerenes have active gas phase reactivities and can be efficiently derivatized by some small organic cations.

扁蕾植物的引种栽培技术

本发明涉及扁蕾植物的引种栽培技术，该技术包括种子处理和引种栽培工艺。本发明通过对扁蕾植物种子进行物理和化学处理，使扁蕾种子萌发率达70%以上；通过地膜覆盖技术，即在种子播种前采用地膜覆盖7-28天，可有效地控制杂草数量和生长，为规模化种植奠定可靠的基础；通过在海拔 1500-4500米的原产水浇大田或适宜旱耕地的引种栽培途径，可快速恢复扁蕾的资源量，并有效地保证扁蕾的药材品质和药效成分。

空值环境下含不确定及可能信息关系数据库的更新

本文在［１］扩展关系模型中引入属性级的不完全信息空值。为适应此环境下更新操作的需要，本文对［１］中的扩展关系模型进行了进一步的扩展，并以此模型为基础讨论了空值环境下含不确定及可能信息关系数据库的更新操作的策略，给出了相应的处理算法。

矿区土壤中重金属活动性评估方法的研究进展

在过去很长一段时间里重金属含量的高低一直都被看作是土壤污染程度的一个重要指标.当化学相的概念被引入到环境科学领域后,人们才逐渐地认识到重金属在环境中的行为和作用,如活动性、生物可利用性、毒性等，用这些金属在环境中的总量来预测和解释是不确切的。为此，许多化学和生物的方法被用来描述土壤与沉积物中重金属的活性。文章对近年来在该领域内的主要研究工作进行了总结，并对其未来可能的发展方向提出了自己的见解。