94 resultados para |Nested-PCR


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Polymerase Chain ReactionPCRPCRPCRPCRPCRPCRPCRPCRPCRPCRPCRPCRPCRPCR20PCRPCR PCRPCRPCRPCRPCR;PCRPCR PCRPCR;PCRPCRPCR;PCRDNAPCR;;PCRPCR PCRNaOHPCRS1DNase I-PCRPCR PCRPCRPCR;DNA;PCR-PCRDNase IPCRPCR PCRPCRPCRPCRPCR; PCRPCR

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To expand the feasibility of applying simple, efficient, non-invasive DNA preparation methods using samples that can be obtained from giant pandas living in the wild, we investigated the use of scent markings and fecal samples. Giant panda-specific oligonucleotide primers were used to amplify a portion of the mitochondrial DNA control region as well as a portion of the mitochondrial DNA cytochrome b gene and tRNA(Thr) gene region. A 196 base pair (bp) fragment in the control region and a 449 bp fragment in the cytochrome b gene and tRNA(Thr) gene were successfully amplified. Sequencing of polymerase chain reaction (PCR) products demonstrated that the two fragments are giant panda sequences. Furthermore, under simulated field conditions we found that DNA can be extracted from fecal samples aged as long as 3 months. Our results suggest that the scent mark and fecal samples are simple, efficient, and easily prepared DNA sources. (C) 1998 Wiley-Liss, Inc.

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Thirteen restriction endonucleases were used to investigate nucleotide sequence variation in the 18S rRNA DNA of 88 individuals from ten Sarcocystis taxa collected as cysts from their intermediate hosts, swine, cattle and water buffalo. A DNA sequence of

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A polymerase chain reaction-based restriction fragment length polymorphism (RFLP) approach is used to examine Sarcocystis cruzi-like taxa from the atypical intermediate host, water buffalo, in Yunnan, People's Republic of China. The loci examined lie with

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DNA, DNA100bp1kbDNAbPCRDNA307bp b(364bp)28 , 4(DraxbaHaeHpa), HaeHap 310

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,PCR-DGGEPCR-DGGE,,;PCR-DGGEPCR-DGGEPCR-DGGE,,DNA