Application of liquid pre-column capillary electrophoresis technique to the study of interaction between drug enantiomers and human serum albumin

Based on the chiral separation of several basic drugs, dimetindene, tetryzoline, theodrenaline and verapamil, the liquid pre-column capillary electrophoresis (LPC-CE) technique was established. It was used to determine free concentrations of drug enantiomers in mixed solutions with human serum albumin (HSA). To prevent HSA entering the CE chiral separation zone, the mobility differences between HSA and drugs under a specific pH condition were employed in the LPC. Thus, the detection confusion caused by protein was totally avoided. Further study of binding constants determination and protein binding competitions was carried out. The study proves that the LPC technique could be used for complex media, particularly the matrix of protein coexisting with a variety of drugs.

应用多质—多评价者程序对评定误差的分析

Two kinds of rating bias ——halo effect and ego-centric effect were analyzed in multitrait-multirater matrix by this research. To get the multitrait-multirater matrix, ten college students watched the video-tapes of football matches and rated the performance of the players on several traits. After the interviewing of 41 football fans, the performance traits of four different positions in a football team were obtained using critical incident technique. The results indicate: comparing the heterotrait-monorater triangless, some rater's rating showed halo effect obviously; comparing the monotrait-heterorater diagonals, ego-centric effect can be shown in some extent on different traits. There were less interrater reliability on the rating of some ambiguous traits. The conclusion can be used on rater training. We can give the raters feedback about their rating bias by analyzing the multitrait-multirater matrix. The results are also helpful for rater training.

Column switching-back flushing technique for the analysis of aromatic compounds in gasoline

A simple method, based on the technique of capillary column switching-back flushing, has been developed for the detailed analysis of aromatic compounds in gasoline. The sample was first separated on a 30-m long OV-2330 polar precolumn and then backflushed onto a nonpolar analytical column. The early eluting components from the precolumn and the components of interest (aromatic compounds plus heavier compounds) eluting from the analytical column are all directed to the same flame ionization detection system through a T piece, which permits the quantitative analysis of aromatic hydrocarbons in gasoline by a normalization method using correcting factors. The switching time window of the method is +/-5 s, resulting in easier operation and higher reliability. The reproducibility of the quantitative analysis was less than or equal to3% RSD for real gasoline samples. (C) 2002 Elsevier Science B.V. All rights reserved.