青藏高原矮嵩草草甸和金露梅灌丛草甸CO2通量变化与环境因子的关系

利用涡度相关技术观测了青藏高原两个典型的生态系统即矮嵩草（Kobresia humilis）草甸和金露梅（Potentilla fruticosa）灌丛草甸的CO2通量，并就2003年8月份的数据，分析了生态系统通量变化与环境因子的关系。8月份是这两个生态系统的叶面积指数达到最高也是相对稳定的时期，在此期间矮嵩草草甸和金露梅灌丛草甸净碳吸收量分别达56．2和32．6g C•m^-2，日CO2吸收量最大值分别为12．7μmol•m^-2•s^-1和9．3μmol•m^-2•s^-1，排放量最大值分别为5．1μmol•m^-2•s^-1和5．7μmol•m^-2•s^-1。在相同光合有效光量子通量密度（PPFD）条件下，矮嵩草草甸CO2吸收速度大于金露梅灌丛草甸；在PPFD高于1200μmol•m^-2•s^-1。的条件下，随气温增加，两生态系统的CO2吸收速度都下降，但矮嵩草草甸的下降速度（-0．086）比金露梅灌丛草甸（-0．016）快。土壤水分影响土壤呼吸，并且影响差异因植被类型不同而不同。生态系统日CO2吸收量随昼夜温差增加而增大；较大的昼夜温差导致较高的净CO2交换量；植物反射率与CO2通量之间存在负相关关系。

Carbon Dioxide Exchange Between the Atmosphere and an Alpine Shrubland Meadow During the Growing Season on the Qinghai-Tibetan Plateau

In the present study, we used the eddy covariance method to measure CO2 exchange between the atmosphere and an alpine shrubland meadow ecosystem (37°36'N, 101°18'E; 3 250 m a.s.l.) on the Qinghai-Tibetan Plateau, China, during the growing season in 2003, from 20 April to 30 September. This meadow is dominated by formations of Potentilla fruticosa L. The soil is Mol-Cryic Cambisols. During the study period, the meadow was not grazed. The maximum rates of CO2 uptake and release derived from the diurnal course of CO2 flux were -9.38 and 5.02 μmol•m-2•s-1, respectively. The largest daily CO2 uptake was 1.7 g C•m-2•d-1 on 14 July, which is less than half that of an alpine Kobresia meadow ecosystem at similar latitudes. Daily CO2 uptake during the measurement period indicated that the alpine shrubland meadow ecosystem may behave as a sink of atmospheric CO2 during the growing season. The daytime CO2 uptake was correlated exponentially or linearly with the daily photo synthetic photon flux density each month. The daytime average water use efficiency of the ecosystem was 6.47 mg CO2/g H2O. The efficiency of the ecosystem increased with a decrease in vapor pressure deficit.

国产6种点地梅属植物的核型及其系统学意义

报道了点地梅属6种12个居群的染色体数目和核型。它们的染色体数目（2n）、核型公式（KF）、染色体相对长度组成（CRL）、核型不对称性系数（A. sK％）和核型类型（KT）分别为：点地梅在南京的2个居群都是2n=2x=20，KF=18m+2sm, CRL=2L+6M_2+10M_1+2S，核型KT属于1A型，但核型不对称性系数As. K％分别是55.04％和56.71％；北点地梅在内蒙古锡林郭勒和白音锡勒居群的2n=2x=20，KF分别为16m+2sm+2st+1b和16m+2sm+2st+4b，染色体相对长度组成分别是14M_2+4M_1+2S+1b和12M_2+6M_1+2S+4b，核型不对称性系数As. K％分别是57.65％和58.86％，属2A型和2B型；高原点地梅在青海玛沁县昌马河居群和兴海县温泉居群都是2n=2x=20，KF=10m+8sm+2st。但相对长度组成分别为12M_2+8M_1和4L+6M_2+6M_1+4S, As. K％分别是60.35％和62.57％，属2A型和2B型；雅江点地梅在青海玛多县巴颜喀拉山居群和大通县达坡山居群分别为2n=4x=40和20n=6x=60，KF=36m+2sm+2st和KF=46m+10sm+4st+2b, CRL=2l+20M_2+14M_1+4S和GRL=6L+30M_2+16M_1+8S+2b, As.K％分别为55.62％和58.31％，核型均属于2B；巴颜喀拉山北坡的鳞叶点地梅2n=40、60、80。西藏点地梅青湖居群中有2种细胞型：①2n=2x=24, KF=12m+6sm+6st+4b, CRL=6L+2M_2+8M_1+4S+4b, As. K％＝65.74％，核型属2B；②2n=2x=22,KF=14m+4sm(2SAT)+4st, CRL=4L+6M_2+8M_1(2SAT)+4S,As. K％=63.40％，核型也属于2B。西宁西山湾居群也有2种细胞型；①2n=3x=36, KF=36m,CRL=4L+12M_2+20M_1, As. K％=54.82％，核型属1A；②2n=3x=33,KF=33m, CRL=3L+12M_2+18M_1,As. K％=52.11％，核型属1A。点地梅属的染色体原始基数可能是x=10，在种间或种内观察到有3种核型变化：染色体非整倍性变化、多倍化和核型不对称性变化。将2倍体居群的核型和不对称性进行比较，可以看出点地梅是较对称的核型。因此，在研究的种中应是比较原始的类群。北点地梅的核型不对称性和进化程度高于点地梅而低于高原点地梅和西藏点地梅。染色体多倍化的雅江点地梅、鳞叶点地梅和西藏点地梅等在核型上也许是最进化的类群。

雅江点地梅（报春花科）3个居群的核型和倍性变化

首次报道了雅江点地梅(Androsace yargongensis) 3 个居群的染色体数目和核型, 对倍性也进行研究。3个居群的染色体数目(2n) , 核型公式(KF) , 染色体相对长度组成(C.RL ), 核型不对称系数(A s. K% ) 和核型类型(KT) 分别为: 野牛沟居群2n= 40; KF = 36 m(1SA T) + 2SM + 2ST + 2b, C. RL = 2L + 14M 2+ 22M 1+ 2S+ 2bS,As.K= 54. 75% , KT = 2A;巴颜喀拉山居群2n= 40, KF = 36 m + 2 sm + 2 st+ 1b, C.RL = 4L + 16M 2+ 18M 1+ 2S+1bS,As.K= 56. 31% , KT = 2B; 达坂山居群2n= 60, KF= 40 m + 14 sm + 6 st, C.RL = 4L +24M 2+ 26M 1+ 6S,As.K= 59. 56% , KT = 2B。根据3 个居群的染色体和核型不对称性与居群所在地的地理位置, 认为雅江点地梅核型和倍性的演化与高海拔生态环境和寒冷、干旱的气候加剧有密切关系。

獐牙菜属5种植物的核型研究（英文）

首次报道了中国5 种獐牙菜属植物的染色体数目和核型。它们染色体中期核型和相对长度组分分别是: 四数獐牙菜为2n= 14= 4m + 8sm + 2st= 2L + 6M 2+ 4M 1+ 2S; 华北獐牙菜2n= 28= 12m + 14sm + 2st= 6L + 8M 2+ 8M 1+ 6S; 二叶獐牙菜为2n= 28= 14m + 4sm +10st= 2L + 14M 2+ 10M 1+ 2S; 抱茎獐牙菜为2n= 20= 6m + 12sm + 2st= 8M 2+ 12M 1; 浙江獐牙菜为2n= 20= 8m + 4sm + 8st= 8M 2+ 12M 1。

昼夜温差对小麦生长特性的影响

在严格控制光照(350μmol photons m -2s-1)和空气相对湿度(75％)的人工气候室内,模拟青海高原昼夜温差较大的环境条件(25℃/10℃,昼/夜),以无昼夜温差的环境条件(25℃/25℃,昼/夜)为对照,对不同环境条件下选育的青海高原338和上海沪麦5号小麦品种的生长发育及经济性状的形成进行了比较研究。结果表明,15℃昼夜温差处理较对照条件下生长的小麦表现较好,总叶面积增加,生育期和叶片寿命延长,植株总干重和每穗粒数及千粒重均显著高于无昼夜温差条件下生长的小麦。因此,较大的昼夜温差环境条件是青海高原小麦易于高产的重要原因。 从品种看,青海的高原338在15℃昼夜温差处理下生长表现显著优于上海的沪 麦5号。而在无昼夜温差条件下，两个品种的生长情况正好相反。由此可以看出，培育适合当地昼夜温差环境因素的品种也是小麦获得高产的主要因素。

马先蒿属6个种的核型与进化研究

分析和报道了马先蒿属（Pedicularis Linn.）分布于青藏高原东北部６个特有种的核型，并根据核型及其有关参数，分析和比较了该６种马先蒿核型的不对称性和相对进化程度。６个种的体细胞染色体数目都是２ｎ＝１６。核型分别为：绵德马先蒿P.pilostachya Maxim.，核型公式Ｋ（２ｎ）＝１６＝４ｍ＋１２ｓｍ，染色体相对长度组成２Ｌ＋６Ｍ２＋６Ｍ１＋２Ｓ，核型不对称系数Ａｓ·Ｋ＝６５．２９％，属于２Ａ型；青海马先蒿P.przewalskii Maxim.，Ｋ（２ｎ）＝１６＝８ｍ（ＳＡＴ）＋４ｓｍ＋２ｓ＋２ｔ，２Ｌ＋８Ｍ２＋２Ｍ１＋４Ｓ，Ａｓ·Ｋ＝６５．０２％，２Ｂ型；华马先蒿P.oederi Vahl.var.sinensis(Maxim.)Hurus.，K(2n)=16=12m+4sm，2L+4M2+8M1+2 S，As·K=59.89%，2B型；粗野马先蒿P.rudis Maxim.，K(2n)=16=4m+10sm+2st，4L+4M2+4M1+2 S，As·K=68.10%，2B型；甘肃马先蒿P.kansuensis Maxim. Subsp. kansuensis，K(2n)=16=6m+6sm+2st+2t，2L+6M2+6M1+2 S，As·K=68.92%，2A型；藓生马先蒿P.muscicola Maxim. K(2n)=16=8m(SAT)+8sm，2L=8M2+4M1+2S，As·K=62.64%，2B型。根据这6个种的核型和已有资料，认为该属的染色体基数x=8，极少数种有多倍体。通过对以上6种核型及进化程度的比较，该属核型变异较大，以中部着丝粒染色体为组成基础（较原始的种类，如华马先蒿和绵穗马先蒿），端部或近端部着丝粒染色体存在与否与该属内种的进化程度有关。核型不对称性所表示的进化程度似乎与花冠的演化有联系。

杜仲光合特性的研究

杜仲（Eucommia ulmoides Oliv）叶片的光饱和光合速率为6～10 μmolCO2·m -2·s-1，表观光合量子需要量最低约为17。光合作用的CO2补偿点略大于100μmolCO2·m -2·s-1，属C3型光合作用类型。其光合日变化有明显的中午降低现象，降低的原因除气孔限制外可能还有光抑制等其它因素。光合作用进行时光合产物只有约14％ 输出叶片.

Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection

On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.

Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography

A novel strategy for the screening and analysis of biologically active compounds in traditional Chinese medicine by molecular biochromatography is proposed. Molecular biochromatography with human serum albumin (HSA) immobilized on silica as stationary phase was used to screen and analyse the bioactive compounds in the typical Chinese medicine of Angelica sinensis (Oliv.) Diels. Ten peaks showed retention on this column, which is based on their affinity for HSA. Ferulic acid and liguistilide were identified as the principal active components, which agrees very well with the results in the literature. A quality control method was also developed based on the simultaneous determination the concentrations of ferulic acid and liguistilide in solutions of Angelica sinensis (Oliv.) Diels extracted with water and methanol. It was observed that the concentrations of ferulic acid and liguistilide in solution extracted with methanol were 2 and 53 times higher, respectively, than those with water. It was shown that molecular biochromatography is an effective way of analysing and screening biologically active compounds in traditional Chinese medicine.

Synthetic studies on optically active Schiff-base ligands derived from condensation of 2-hydroxyacetophenone and chiral diamines

Three optically active Schiff-base ligands have been prepared by condensation of 2-hydroxyacetophenone with (IR,2R)-(-)-1,2-diaminocyclohexane, (1S,2S)-(-)1,2-diphenylethylenediamine or R-(+)-2,2'-diamino-1,1'-binaphthalene, respectively. The products have been characterized by their IR, H-1- and C-13-NMR spectra.

Homogeneous time-resolved fluoroimmunoassay of bensulfuron-methyl by using terbium fluorescence energy transfer

A sensitive homogenous time-resolved fluoroimmunoassay (TR-FIA) method for bensulfuron-methyl (BSM) based on fluorescence resonance energy transfer (FRET) from a Tb3+ fluorescent chelate with N,N,N',N'-[2,6-bis(3'-aminomethyl-1'-pyrazoly)-4-phenylpyridine] tetrakis(acetic acid) (BPTA-Tb3+) to organic dye, Cy3 or Cy3.5 has been developed. New method combined the use of BPTA-Tb3+ labeled streptavidin, Cy3 or Cy3.5 labeled anti-BSM monoclonal antibody and biotinylated BSM-BSA conjugate (BSA is bovine serum albumin) for competitive-type immunoassay. After BPTA-Tb3+ labeled streptavidin was reacted with a competitive immune reaction solution containing biotinylated BSM-BSA, BSM sample and Cy3 or Cy3.5 labeled anti-BSM monoclonal antibody, the sensitized and long-lived emission of Cy3 or Cy3.5 derived from FRET was measured, and thus the concentration of BSM in sample was calculated. The present method has the advantages of rapidity, simplicity and high sensitivity since the B/F (bound reagent/free reagent) separation steps and the solid-phase carrier are not necessary. The method gives the detection limit of 2.10 ng ml(-1). The coefficient variations of the method are less than 1.5% and the recoveries are in the range of 95-105% for BSM water sample measurement. (C) 2001 Elsevier Science B.V. All rights reserved.

Modeling of protein adsorption in membrane affinity chromatography

For the design of affinity membranes, protein adsorption in membrane affinity chromatography (MAC) was studied by frontal analysis. According to fast mass transfer, small thickness of affinity membranes and high affinity between the protein and the ligand, an ideal adsorption (IA) model was proposed for MAC and was used together with equilibrium-dispersive (E-D) model to describe the adsorption of bovine serum albumin (BSA) onto cellulose diacetate/polyethyleneimine (CA/PEI) blend membranes with and without Cu2+ chelating. E-D model was found to better describe the initial region of experimental breakthrough curves. The influence of axial dispersion was revealed and it showed the importance of design of the module to homogenously distribute feed solution. IA model was found to be better for the whole experimental breakthrough curve. According to it, the capacity of affinity membranes and the specificity of the interaction are of equal importance for the design of affinity membranes. An optimum feed concentration was also found in the operation of MAC. The discrepancy between experimental optimum feed concentrations and predicted ones from IA model may be due to the ignorance of some experimental effects such as axial dispersion.

Evaluation of nonlinear chromatographic performance by frontal analysis using a simple multi-plate mathematical model

A multi-plate (NIP) mathematical model was proposed by frontal analysis to evaluate nonlinear chromatographic performance. One of its advantages is that the parameters may be easily calculated from experimental data. Moreover, there is a good correlation between it and the equilibrium-dispersive (E-D) or Thomas models. This shows that it can well accommodate both types of band broadening that is comprised of either diffusion-dominated processes or kinetic sorption processes. The MP model can well describe experimental breakthrough curves that were obtained from membrane affinity chromatography and column reversed-phase liquid chromatography. Furthermore, the coefficients of mass transfer may be calculated according to the relationship between the MP model and the E-D or Thomas models. (C) 2004 Elsevier B.V. All rights reserved.

Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC

Interaction of traditional Chinese Herb Rhizoma Chuanxiong and protein was studied by microdialysis coupled with high performance liquid chromatography. Compounds in Rhizoma Chuanxiong, such as ferulic acid, senkyunolide A and 3-butylphthalide, were identified by HPLC, HPLC-MS and UV-vis. Microdialysis recoveries and binding degrees of compounds in Rhizoma Chuanxiong with human serum albumin (HSA) and other human plasma protein were determined: recoveries of microdialysis sampling ranged from 36.7 to 98.4% with R.S.D. below 3.1%; while binding to HSA ranged from 0 to 91.5% (0.3 mM HSA) and from 0 to 93.5% (0.6 mM HSA), respectively. Compared with HSA, most of compounds bound to human blood serum more extensively and the results showed that binding of these compounds in Rhizoma Chuanxiong was influenced by pH. Two compounds were found to bind to HSA and human blood serum. their binding degrees were consistent with ferulic acid and 3-butylphthalide, the active compounds in Rhizoma Chuanoxiong. (c) 2005 Elsevier B.V. All rights reserved.