基于多矩的有限体积浸入边界法研究

<span style="font-family: 'Trebuchet MS', 'Lucida Sans Unicode', Arial, sans-serif; line-height: 22px">浸入边界法（Immersed Boundary Method）是计算流体力学中求解具有复杂、移动边界流动问题的一类有效途径，该方法在笛卡尔坐标系上离散求解流体控制方程，并通过在控制方程中添加相应源相来代表浸入边界。尽管浸入边界法借助其简单、高效的显著特点在计算流体力学应用中显示出极强的生命力，特别是针对复杂的实际流动及动边界流动问题有着无可比拟的优势，但仍有许多问题需要进一步的研究。 本论文基于浸入边界方法及多矩VSIAM3（Volume/Surface Integrated Average Multi-Moment Method）格式提出了一种不可压缩流体求解数值格式。不可压N-S方程使用VSIAM3格式进行法进行离散，引入浸入边界法处理复杂、移动流动边界条件，使用虚拟网格方法计算动量方程修正项，同时还考虑了对连续方程的修正。VSIAM3格式是一种基于多矩的有限体积法，在方程的离散中总是使用两种或两种以上的矩，如：VIA（Volume Integrated Average）和SIA（Surface Integrated Average）。而不同的矩在求解过程中依据不同形式的控制方程使用不同的离散方法进行更新。VSIAM3格式更多的局地自由度及同时使用交错网格和同位网格的特点使浸入边界法的实施更加便利、高效。研究中，浸入边界法不仅应用于处理动力边界条件，同样可以处理热动力边界条件。 研究中对大量经典算例进行了数值实验，包括一维线性初始问题、方腔流问题、二维绕静止及振荡圆柱流动、三维绕球流动及热对流问题等。数值结果同实验值及其它计算结果保持一致，该算法可准确、高效处理具有复杂、移动边界及存在热对流的不可压流动问题，为实际应用打下了基础。span>

Flow-induced vibrations of long circular cylinders modeled by coupled nonlinear oscillators

The dynamics of long slender cylinders undergoing vortex-induced vibrations (VIV) is studied in this work. Long slender cylinders such as risers or tension legs are widely used in the field of ocean engineering. When the sea current flows past a cylinder, it will be excited due to vortex shedding. A three-dimensional time domain model is formulated to describe the response of the cylinder, in which the in-line (IL) and cross-flow (CF) deflections are coupled. The wake dynamics, including in-line and cross-flow vibrations, is represented using a pair of non-linear oscillators distributed along the cylinder. The wake oscillators are coupled to the dynamics of the long cylinder with the acceleration coupling term. A non-linear fluid force model is accounted for to reflect the relative motion of cylinder to current. The model is validated against the published data from a tank experiment with the free span riser. The comparisons show that some aspects due to VIV of long flexible cylinders can be reproduced by the proposed model, such as vibrating frequency, dominant mode number, occurrence and transition of the standing or traveling waves. In the case study, the simulations show that the IL curvature is not smaller than CF curvature, which indicates that both IL and CF vibrations are important for the structural fatigue damage.

Two-dimensional Vortex-induced Vibrations of Submerged Floating Tunnel Tethers

A vortex-induced vibration (VIV) model is presented for predicting the nonlinear dynamic response of submerged floating tunnel (SFT) tethers which are subjected to wave, current and tunnel oscillatory displacements at their upper end in horizontal and vertical directions. A nonlinear fluid force formula is introduced in this model, and the effect of the nonlinearity of tether is investigated. First, the tunnel is stationary and the tether vibrates due to the vortices shedding. The calculated results show that the cross-flow amplitude of VIV decreases compared with the linear model. However the in-line amplitude of VIV increases. Next, the periodical oscillation of tunnel is considered. The oscillation caused by wave forces plays the roles of parametric exciter and forcing exciter to the VIV of tether. The time history of displacement of the tether mid-span is obtained by the proposed model. It is shown that the in-line amplitude increases obviously and the corresponding frequency is changed. The cross-flow amplitude exhibits a periodic behavior.

聚二甲基硅氧烷（PDMS）表面液滴动力学的实验研究

<span style="font-family: 'Trebuchet MS', 'Lucida Sans Unicode', Arial, sans-serif; line-height: 22px">液滴是自然界中普遍存在的一种物质形态。非连续微流体（液滴）是近年来微流体技术重要发展方向之一。对液滴的产生、启动、移动、合并、分离和碰撞过程的研究对于航天、微纳系统、电子显示、计算机冷却、喷墨、生物医学等学科领域有着重要的应用价值。液滴属于软物质，其力学性质介于流体和固体之间，其类固体（solid-like）行为来自于曲率产生的Laplace压力和表面张力的约束。对液滴动力学行为的研究有着重要的学术价值。 本文的主要工作是针对生物微电子机械系统（Bio-MEMS）以及柔性微纳电子加工中常用的材料聚二甲基硅氧烷（Polydimethylsiloxane，PDMS）为基底的液滴动力学实验研究。 液滴是一个理想的微反应器，许多实验可以集成在一个液滴或多个液滴内完成。液滴本身的动力学特性对于实验的完成效率和质量有着重要的影响。液滴的微操控技术包括多相流法、电润湿法、热毛细管法、介电泳法等。液滴的动力学特性受到基底的影响非常大，包括基频、振动模态、运动过程等均随基底的润湿性、弹性模量的变化而有所变化。 在Bio-MEMS以及柔性微纳电子加工当中，PDMS扮演着越来越重要的角色，尤其是PDMS的润湿性和电润湿特性。目前的PDMS在Bio-MEMS当中主要是用于制备各种微流道。常见的问题主要是一方面PDMS是疏水材料，影响流体的输运。另一方面是液体在这种低Reynolds数情况下不易混合，反应效率低。本文提出了在PDMS表面溅射纳米厚度的金来减小PDMS表观接触角的方法。这种方式在特定喷金量的情况下可以在PDMS表面产生多层次的压应力波纹。这种压应力波纹对于柔性微纳电子加工，以及微流道中加速流体混合有着非常重要的作用。 电润湿是另一种可以使PDMS亲水化的方法。实验证明，PDMS具有较好的电润湿性质。此外电润湿也是目前操纵液滴的主要方式。目前一个常见的问题是电击穿现象阻碍了驱动电压的低压化，且低Reynolds数情况下液滴的混合效率偏低。此外电极还会由于少量电解的发生导致腐蚀及对液体样品的污染。本文提出了接触式的电润湿，在电极逐渐触碰液滴的过程中，液滴发生百Hz的失稳振动，稳定后接触角减小。这种电润湿模式可以有效的提高临界击穿电压，避免液滴被腐蚀后的电极污染，同时可以加快液滴的混合效率。其失稳特征时间在10 ms量级，这恰是所用液滴特征尺度在1 mm左右的电润湿器件的最快响应时间。并采用液滴振动的理论估算了液滴的失稳时间，同时还考虑了基底润湿性对液滴振动过程的影响。 液滴的启动是电润湿操控液滴过程中的重要环节。通常的液滴启动都是在非连续基底上依靠逻辑电路产生的电势变化来驱动液滴。无论是逻辑电路的设计还是驱动装置的加工都非常复杂。本文首次实现了在超疏水生物样品荷叶上的液滴启动，启动速度为数十毫米/秒，启动时间为10 ms量级。并利用PDMS成功的仿制了荷叶结构实现了超疏水的PDMS表面，荷叶同仿荷叶的PDMS超疏水表面具有相近的润湿性。 在数字微流体操控液滴的过程中，液滴的合并涉及液滴的碰撞，而且MEMS系统当中利用液滴撞击进行冷却的实验已经有所开展。同时理解液滴碰撞还对许多领域包括生物、化学、喷墨、大气物理等有着非常重要的作用。本文实验研究了Weber数和毛细数对液滴碰撞过程的影响，通过改变Weber数和毛细数得到了四种不同的响应模式。span>

Configuration optimization of hypersonic vehicles under transitional flow conditions

The rarefied gas effects on several configurations are investigated under hypersonic flow conditions using the direct simulation Mont Carlo method. It is found that the Knudsen number, the Mach number, and the angle of attack all play a mixed role in the aerodynamics of a flat plate. The ratio of lift to drag decreases as the Knudsen number increases. Studies on 3D delta wings show that the ratio of lift to drag could be increased by decreasing the wing thickness and/or by increasing the wing span. It is also found that the waveriders could produce larger ratio of lift to drag as compared with the delta wing having the same length, wing span, and cross section area.

IP方法模拟硬盘读写头气膜润滑问题

<span style="font-family: 'Trebuchet MS', 'Lucida Sans Unicode', Arial, sans-serif; line-height: 22px">本论文中采用IP方法模拟硬盘读写头的气膜润滑问题，并选择耗时但精准的DSMC方法作为该问题中IP方法的检验标准，IP方法计算得到的读写头表面压力分布及读写头所受净升力为读写头的设计提供可靠的参考数据。 首先，将读写头复杂表面简化为平面并假定尺寸为微米（远小于真实的毫米），微米尺寸时可以得到DSMC的模拟结果用于比较。IP法对该问题的模拟结果与DSMC的模拟结果完全吻合。 其次，选定一个真实的读写头表面几何，但仍然假定尺寸为微米。IP法在该问题的应用过程中遇到四点困难，我们提出或采取了相应的解决办法。在复杂表面几何的读写头气膜润滑问题中，IP法的模拟结果与DSMC的模拟结果也相符一致。 最后完成真实几何、真实尺寸（毫米）的读写头气膜润滑问题的模拟，这也是本论文的最终研究目标。IP法模拟该问题时需要采用大网格，为此我们构造二维检验模型验证了大网格的合理性。 真实几何读写头气膜润滑问题的模拟结果表明，在流场的局部区域压力沿着高度方向变化显著，这意味着读写头问题的传统计算方法（即概括性雷诺方程）在该局部区域不成立，故用它计算得到的结果值得认真检验，但文献中尚未给出过这类检验。这正是该论文的研究背景和采用新方法（IP方法）的原因。 本论文研究的问题属于过渡领域、低速流动问题，故本论文还介绍了该领域另外一种粒子模拟方法（LVDSMC方法）以及我们对该方法提出的一些改进。 关键词：硬盘读写头，气膜润滑，概括性雷诺方程，IP方法，DSMC方法，过渡领域，LVDSMC方法span>

冲压发动机喷气催化复合效应研究

<span style="font-family: 'Trebuchet MS', 'Lucida Sans Unicode', Arial, sans-serif; line-height: 22px">在高超声速飞行条件下，流入冲压发动机燃烧室并降至低速的空气的温度随飞行马赫数增加而愈来愈高。燃料与高温空气混合燃烧释放的化学能中的一部分将转化为解离能。这些解离能在长度受限的尾喷管中难以充分复合形成推力，使冲压发动机推力在高超声速范围内随飞行马赫数增大而下降，难以满足高超声速飞行器的推进要求。 与亚燃冲压发动机相比，流入超燃冲压发动机燃烧室的空气的温度在同样飞行马赫数条件下将明显降低，上述困难可大大缓解。然而目前超燃冲压发动机还存在关键性难点有待克服。若保持现有亚燃冲压发动机的吸气与燃烧方式，通过催化促进燃气解离组分在尾喷管膨胀过程中复合，可以增大冲压发动机的推力，满足高超声速飞行器的推进要求，为高超声速飞行器推进提供新的选择。 本论文主要研究内容如下： （1） 研究了亚燃冲压发动机燃烧室内燃气解离能与飞行马赫数的关系。通过对冻结流、平衡流和有限化学反应速率的流动的数值计算，确定了回收解离能增大推力的潜力。 （2） 以双爆轰技术为基础，建立起一套地面燃气产生装置。所产生的燃气的组分、温度和压力均与冲压发动机在高空飞行时燃气完全相同。调试出总温3200K、总压20Bar（对应来流马赫数6）和试验时间17.5ms以及总温4000K、总压5Bar（对应来流马赫数8）和试验时间12.5ms两种状态参数的试验用燃气。 （3） 建立了基于动量守恒原理的通过皮托管测压力换算推力的测量方法。对催化复合增大推力的实验而言，一般要进行特定流动条件下喷水与未喷水两种情况下推力大小的比较，其精度可以达到2%甚至更高。 （4） 完成了尾喷管喉道下游管壁喷水试验，成功释放出高温燃气中的解离能，有效增大了推力，证实了催化增推的想法是可行的。在来流马赫数6的条件下获得了11.0%的推力增量；在来流马赫数8.0的条件下也获得了11.7%的推力增加。span>

The Experimental and Numerical Studies on Gas Production from Hydrate Reservoir by Depressurization

A set of experimental system to study hydrate dissociation in porous media is built and some experiments on hydrate dissociation by depressurization are carried out. A mathematical model is developed to simulate the hydrate dissociation by depressurization in hydrate-bearing porous media. The model can be used to analyze the effects of the flow of multiphase fluids, the kinetic process and endothermic process of hydrate dissociation, ice-water phase equilibrium, the variation of permeability, convection and conduction on the hydrate dissociation, and gas and water productions. The numerical results agree well with the experimental results, which validate our mathematical model. For a 3-D hydrate reservoir of Class 3, the evolutions of pressure, temperature, and saturations are elucidated and the effects of some main parameters on gas and water rates are analyzed. Numerical results show that gas can be produced effectively from hydrate reservoir in the first stage of depressurization. Then, methods such as thermal stimulation or inhibitor injection should be considered due to the energy deficiency of formation energy. The numerical results for 3-D hydrate reservoir of Class 1 show that the overlying gas hydrate zone can apparently enhance gas rate and prolong life span of gas reservoir.

Abnormal phenomenon of ac Stark splitting in magneto-optical traps

We experimentally study the ac Stark splitting in D2 line of cold Rb-87 atoms. The frequency span between the Autler-Townes doublets is obviously larger than that derived from theoretical calculation. Two physical effects, which increase the effective Rabi frequency, contribute to the splitting broadening. First, atoms tend to distribute in strong lield places of a inhomogeneous red-detuned light field. Second, atoms reabsorb scattered light when they are huge in number and high in density.

一种同时测量温度和应变的光纤光栅传感器

报道了一种新型实用的用单根光纤布拉格光栅(FBG)实现温度和应变分离传感的技术。当光纤光栅一部分包层直径变小时，整个光栅可以看成由两个周期相同但直径不同的子光栅连接而成。理沦分析和实验都证实了这两个子光栅具有相同的温度敏感性和不同的应变敏感性．由此实现光纤光栅传感器中温度和应变两参数的分离测量，而且这两个子光栅的中心波长间距可以直接测量应变大小．温度变化不影响所测量的应变值。实验中光栅的一部分包层直径被HF酸腐蚀到82μm．获得了两子光栅应变响应系数分别为0．00201nm/με．0．000858nm/μ

Absence of association between SNPs in the promoter region of the insulin-like growth factor 1 (IGF-1) gene and longevity in the Han Chinese population

Previous studies have indicated that genetic variations in the factors of insulin/insulin-like growth factor 1 (IGF-1) signaling pathway could influence human life-span by affecting IGF-1 levels. The promoter region of the IGF-1 gene is an obvious candida

THE POSSIBLE INVOLVEMENT OF TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN LUTEOLYSIS OF RHESUS-MONKEY

Changes of plasminogen activators (PA) during different stages of development of the corpus luteum, and their possible physiological role in luteolysis were studied in rhesus monkeys. It was demonstrated for the first time that monkey corpus luteal cells not only produce PA, but that the function of the corpus luteum is also closely related to the activity of this enzyme system. Generally, the life span for a corpus luteum in monkey is approximately 14-16 days, its demise beginning thereafter. In the present study, we found that urokinase in the corpus luteum is higher on day 5 and day 10 after human chorionic gonadotrophin injection, while the tissue type (t) PA is mainly produced on day 13 when luteolysis may take place. Progesterone production remained high on day 5 and day 10 and decreased dramatically from day 13, indicating the important role of tPA but not urokinase (u) PA in suppressing luteal function. When purified tPA (but not uPA) monoclonal antibody was added to luteal cell culture to neutralize endogenously produced tPA activity, progesterone production in the cells was increased significantly. Interestingly, prolactin alone was capable of increasing PA production by luteal cells; prolactin together with luteinizing hormone, however, had a synergistic luteotrophic effect.

Effects of exogenous double-stranded RNA on the basonuclin gene expression in mouse oocytes

In plants and less-advanced animal species, such as C.elegans, introduction of exogenous double-stranded RNA (dsRNA) into cells would trigger degradation of the mRNA with homologous sequence and interfere with the endogenous gene expression. It might represent an ancient anti-virus response which could prevent the mutation in the genome that was caused by virus infection or mobile DNA elements insertion. This phenomenon was named RNA interference, or RNAi. In this study, RNAi was used to investigate the function of basonuclin gene during oogenesis. Microinjection of dsRNA directed towards basonuclin into mouse germinal-vesicle-intact (GV) oocytes brought down the abundance of the cognate mRNA effectively in a time- and concentration-dependent manner. This reduction effect was sequence-specific and showed no negative effect on other non-homologous gene expression in oocytes, which indicated that dsRNA can recognize and cause the degradation of the transcriptional products of endogenous basonuclin gene in a sequence-specific manner. Immunofluorescence results showed that RNAi could reduce the concentration of basonuclin protein to some extent, but the effect was less efficient than the dsRNA targeting towards tPA and cMos which was also expressed in oocytes. This result might be due to the long half life of basonuclin protein in oocytes and the short reaction time which was posed by the limited life span of GV oocytes cultured in vitro. In summary, dsRNA could inhibit the expression of the cognate gene in oocytes at both mRNA and protein levels. The effect was similar to Knock-out technique which was based on homologous recombination. Furthermore, hairpin-style dsRNA targeting basonuclin gene could be produced by transcription from a recombinant plasmid and worked efficiently to deplete the cognate mRNA in oocytes. This finding offered a new way to study the function of basonuclin in the early stage of oogenesis by infection of primordial oocytes with the plasmid expressing hairpin-style basonuclin dsRNA.

Molecular modeling on human CCR5 receptors and complex with CD4 antigens and HIV-1 envelope glycoprotein gp120

AIM: To investigate the interaction between human CCR5 receptors (CCR5) and HIV-1 envelope glycoprotein gp120 (HIV-1 gp120) and HIV-1 receptor CD4 antigens (CD4). METHODS: The structurally con served regions (SCR) of human CCR5 was built by the SYBYL/Biopolymer module using the corresponding transmembrane (TM) domain of bacteriorhodopsin (bR) as the template. The coordinates for amino-ter minal residue sequence, and carboxyl-terminal residue sequence, extracellular and cytoplasmic loops were generated using LOOP SEARCH algorithm. Subsequently the structural model was merged into the complex with HIV-1 gp120 and CD4. RESULTS: Human CCR5 interacted with both an HIV-1 gp120 and CD4. The N-terminal residues (especially Met1 and Gln4) of human CCR5, contacted with CD4 residues, mainly 7Nith one span (56 - 59) of CD4 in electrostatic interaction and hydrogen-bonds. The binding sites of human CCR5 were buried in a hydrophobic center surrounded by a highly basic periphery. On the other hand, direct interatomic contacts were made between ? CCR5 residues and 6 gp120 amino-acid residues, which included van der Waals contacts, hydrophobic interaction, and hydrogen bonds. CONCLUSION: The interaction model should be helpful for rational design of novel anti-HIV drugs.

The effect of food concentration on the life history of three types of Brachionus calyciflorus females

The effect of food concentration on the life history of three types of Brachionus calciflorus females (amictic, unfertilized mictic and fertilized mictic female) was studied with replicated individual cultures at 25 degrees and at four food concentrations (1.5, 3.0, 6.0 and 9.0 x 10(6) cells mL(-1)) of Scenedesmus obliquus. There were highly significant effects of both food concentration and female type, independently and in interaction on the duration of juvenile period of the rotifer, but neither a;ere the effects on the duration of post-reproductive period and mean life-span. The duration of juvenile period of unfertilized mictic female at the food concentration of 9.0 x 10(6) cells mL(-1) was the longest among all the food concentration-female type combinations. Both food concentration and female type influenced significantly the duration of reproductive period and the number of eggs produced by each type of female per life cycle, respectively. There was, however, no significant interaction between food level and female type. Among the three types of females, the number of eggs produced by an unfertilized mictic female was the largest. and that of a fertilized mictic female was the smallest.