Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice

Laurencia terpenoid extract (LET) had been extracted from the red alga Laurencia tristicha. The study is to investigate the effects of LET supplementation on DNA oxidation and alkylation damages in mice. Forty healthy kunming mice weighing between 18g and 25g were randomly assigned into 4 groups, each consisting of ten animals. The mice were orally intubated respectively for 60 days with the designed concentrations of LET (25, 50, 100 mg/kg b.w.) for three exposed groups and salad oil (0.2 ml) for the blank group. Food and water were free for the animals. Mice in the blank and exposed groups were sacrificed after the last treatment and the blood of each animal was quickly taken for further experiments. The spontaneous and oxidized DNA damages of peripheral lymphocytes induced by H2O2 were analysed by SCGE. O-6-Methy-guanine (O-6-MeG) was measured by high performance capillary zone electrophoresis. There was no significantly difference in DNA spontaneous damage on peripheral lymphocytes of all the mice. The oxidative DNA damage in the 50 mg/Kg body weight supplement group are 286AU with the oxidation of 10 mu mol/L H2O2, significantly lower than the blank group 332AU (p<0.05). The contents of O-6-MeG in plasma in the 50mg/kg b.w. and 100mg/kg b.w. supplement group were 1.50 mu mol/L andl.88 mu mol/L, significantly lower than that of the blank group, which was 2.89 mu mol/L(p<0.05). The results from the present study indicated that the LET were rich in terpenoids and safety to be taken orally and it could improve antioxidative and decrease DNA damage effectively.

CHINESE NITRARIA TANGUTORUM BOBR。： CHEMICAL CONSTITUENTS OF SEED OIL EXTRACTED BY SFE-CO_2

In order to develop and make good use of Nitraria tangutorum Bobr. in Qinghai-Tibetan Plateau for its ecological and medicinal values, the seed oil was extracted by SFE-CQ2 and the chemical constituents was analyzed by GC/MS. The component relative contents were determined by area nomalization. 28 components were separated from the extracts of SFE-CQj and 12 of them, which accounted for 85.99% were identified. They were(Z, Z)-9, 12-octadecadienoic acid (linoleic acid), bicyclo[ 10. 1. 0] tridec-1-ene, 7-pentadecyne, gamma-sitosterol, gamma-tocopherol, 1, -8,Z-10-hexadecatriene,9,12-octadecadienal, 24-methyl-5-cholestene-3-ol,(Z)-9,17-octadecadienal, stigmastan-3,5-dien, eicosane and so on. Among them, the relative content of (Z, Z)-9,12-octadecadienoic acid is the highest, accounting for 65.85% of the total area. It is concluded that N. tangutorum Bobr. seed oil is a rich source of linoleic acid.

Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection

The seed oil from Nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. The extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. The free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as a labeling regent, followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (APCI in positive-ion mode). HPLC analysis shows that the main compositions of the seed oil samples were free fatty acids (FFAs) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. The assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/L. Excellent linear responses were observed, with correlation coefficients greater than 0.999. The facile BDETS derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical CO2 extraction. The established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (C) 2007 Elsevier B.V. All rights reserved.

Photoperiodic regulation in energy intake, thermogenesis and body mass in root voles (Microtus oeconomus)

The present study was designed to examine whether photoperiod alone was effective to induce seasonal regulations in physiology in root voles (Microtus oeconomus) from the Qinghai-Tibetan plateau noted for its extreme cold environment. Root voles were randomly assigned into either long photoperiod (LD; 16L: 8D) or short photoperiod (SD; 8L: 16D) for 4 weeks at constant temperature (20 degrees C). At the end of acclimation, SD voles showed lower body mass and body fat coupled with higher energy intake than LD voles. SD greatly enhanced thermogenic capacities in root voles, as indicated by elevated basal metabolic rate (BMR), nonshivering thermogenesis (NST), mitochondrial protein content and uncoupling protein-1 (UCP1) content in brown adipose tissue (BAT). Although no variations in serum leptin levels were found between SD and LD voles, serum leptin levels were positively correlated with body mass and body fat mass, and negatively correlated with energy intake and UCP1 content in BAT, respectively. To summarize, SD alone is effective in inducing higher thermogenic capacities and energy intake coupled with lower body mass and body fat mass in root voles. Leptin is potentially involved in the photoperiod induced body mass regulation and thermogenesis in root voles. (c) 2006 Elsevier Inc. All rights reserved.

Seasonal thermogenesis and body mass regulation in plateau pikas (Ochotona curzoniae)

Changes in photoperiod, ambient temperature and food availability trigger seasonal acclimatization in physiology and behavior of many animals. In the present study, seasonal adjustments in body mass and in several physiological, hormonal, and biochemical markers were examined in wild-captured plateau pikas (Ochotona curzoniae) from the Qinghai-Tibetan plateau. Our results showed that plateau pikas maintained a relatively constant body mass throughout the year and showed no seasonal changes in body fat mass and circulating levels of serum leptin. However, nonshivering thermogenesis, cytochrome c oxidase activity, and mitochondrial uncoupling protein 1 (UCP1) contents in brown adipose tissues were significantly enhanced in winter. Further, serum leptin levels were positively correlated with body mass and body fat mass while negatively correlated with UCP1 contents. Together, these data suggest that plateau pikas mainly depend on increasing thermogenic capacities, rather than decreasing body mass, to cope with cold, and leptin may play a potential role in their thermogenesis and body mass regulation.

Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau

Leptin, an adipocyte-derived hormone, plays all important role in body energy homeostasis. Plateau pika (Ochotona curzoniae), an endemic and keystone species living only at 3000-5000 in above sea level oil Qinghai-Tibet Plateau, is a typically high hypoxia and low temperature tolerant mammal with high resting metabolic rate (RMR), non-shivering thermogenesis (NST), and high ratio of oxygen utilization to cope with harsh plateau environment. To explore the molecular mechanism of ecological acclimation in plateau pika, we first cloned pika leptin cDNA and compared its mRNA expression in different altitudes (3200 and 3900 in) using real-time RTPCR (Taqman probe) technology. The full-length pika leptin cDNA was 3015 with 504 bp open-reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. Pika leptin was 70-72% homologous to that of other species and was of similarly structural characteristics with other species. The pika-specific genetic diversity in leptin sequence occurred at twenty sites. With the increase in attitude, there were larger fat store and high level of ob gene expression in plateau pika. Our results indicated that leptin is sensitive to cold and hypoxia plateau environment and may play one of important roles in pika's ecological adaptation to harsh plateau environment. (c) 2006 Elsevier Inc. All rights reserved.

Seasonal changes in weight and body composition of yak grazing on alpine-meadow grassland in the Qinghai-Tibetan plateau of China

Forty-five male yaks (born April 2001) were studied to determine how seasonal changes on the Qinghai-Tibetan plateau affected BW and body composition. Thirty yaks were weighed monthly from birth to 26 mo of age to determine seasonal changes in BW. The remaining 15 yaks were allocated randomly to five groups (three yaks per group), designated for slaughter at 13, 15, 18, 22, and 25 mo to measure seasonal effects on body chemical composition. All yaks were grazed on the alpine-meadow grassland of the plateau without any supplementation. All BW and body composition data were calculated on an individual basis. Body weight and body composition data were both compared across seven growth periods spanning 2 yr and defined by season. From April (birth) to December 2001 of the first growing season, yak BW increased (P < 0.01); however, during the subsequent cold season (December 2001 to May 2002), BW decreased (P < 0.01). The second growing season ran from May 2002 (13 mo of age) to October 2002 (18 mo of age), and the second live weight-loss season ran from October 2002 until May 2003. The weight loss experienced by yaks during the first weight loss season was 25.64% of the total weight gain in the first growing season. The weight loss experienced by yaks during the second weight-loss season was 29.73% of the total weight gain in the second growing season. Energy retention in the second growing season was 291.07 MJ, 50.8% of which was consumed during the subsequent cold season. Energy accumulation in the summer (from May to July) and fall (from July to October) of the second growing season did not differ (5.01 and 6.30 MJ/kg of EBW gain, respectively; P = 0.63). The energy mobilized during the second winter (from October 2002 to February 2003) was 16.49 MJ/kg of EBW, and in the second spring (from February to May 2003), it was 9.06 MJ/kg of EBW. These data suggest that the decrease in grazing yak BW during the first cold season is much less than during the second cold season, and that the energy content per unit of BW mobilized is greater (P = 0.02) in winter than in spring. Results from this study demonstrate highly efficient compensatory growth in grazing yaks following the first weight loss period during the first cold season. This benefit could be exploited by herders to improve yak production. Yaks may have developed a type of self-protection mechanism to overcome the long cold seasons in the Qinghai-Tibetan plateau.

柴达木盆地地震勘探关键技术研究

The rugged surface topography determined the seismic data acquisition construction conditions and the seismic wave explosive and receiver quality in Qaidam Basin. This dissertation systematically researched the seismic acquisition, imaging process and the attribute analysis techniques of complicated oil and gas reservoir. The main research achievements and cognitions are as follows: 1. Through the stimulation effects research and analysis from the aspect of lithologic water-containing differences, it’s specific that stable hydrous sand layer can effectively enhance the stimulation effects combined with the corresponding field tests. The seismic data S/N ratio has been improved due to the combination explosive stimulation. Through the fold number and maximum offset analyses of target horizon, the complicated geometry has been optimized and the S/N ratio of seismic data has been improved, which made an important basis for improvement of 3D seismic data. 2. It has been proved that the first arrival refraction static correction method under the model constraint of fine surface survey is suitable to the Qaidam Basin of western areas by the real seismic data processing. Although the refraction horizon of near surface has some changes in a certain extent, it’s steady basically. The refraction horizon can be continuously traced in sections, so it’s qualified for the refraction static correction method on the whole. 3. The research is based on the curved-ray pre-stack time migration techniques of rough topography, and improved the imaging precision of complex areas. This techniques adopted the constant and variable velocity scanning mode and enhanced the velocity analysis precision. The 3D pre-stack time migration techniques reasonably solved the imaging and velocity multiple solutions problems of steep-dip faults and the intersections of horizontal layers. What’s more, fine velocity analysis and mute are very important to enhance the imaging precision of the seismic data in complicated Wunan areas. 4. The 3D seismic data edge-preserving processing methods have been realized due to the image process techniques. Because this method uses the large range filter, it can attenuate the noise maximally. The faults, break points, lithologic pinchout points and lithologic body of small scale such as river will not be influenced by blur because of the edge-preserving characterization of the method which is really an effective assistant technique of low S/N ratio seismic data attribute analysis. 5. The use of spectral decomposition technique can effectively identify the reservoirs. The special geology body which will not be identified (or without obvious characters) in the seismic profile may be found through the details changes of different frequencies in the amplitude profiles.

黄骅坳陷港西开发区河流相储层构型与剩余油分布模式研究

In the production tail of oilfield, water-cut is very high in thick channel sand oil reservoir, but recovery efficiency is relative low, and recoverable remaining oil reserves is more abundant, so these reserves is potential target of additional development. The remaining oil generally distributed with accumulation in certain areas, controlled by the reservoir architecture that mainly is the lateral accretion shale beddings in the point bar, so the study of reservoir architecture and the remaining oil distribution patterns controlled by architecture are very significant. In this paper, taking the Minghuazhen formation of Gangxi oilfield as a case, using the method of hierarchy analysis, pattern fitting and multidimensional interaction, the architecture of the meandering river reservoir is precisely anatomized, and the remaining oil distribution patterns controlled by the different hierarchy architecture are summarized, which will help to guide the additional development of oil fields. Not only is the study significant to the remaining oil forecasting, but also it is important for the theory development of reservoir geology. With the knowledge of sequence correlation and fluvial correlation model, taking many factors into account, such as combination of well and seismic data, hierarchical controlling, sedimentary facies restraint, performance verification and 3-D closure, an accurate sequence frame of the study area was established. On the basis of high-resolution stratigraphic correlation, single layer and oil sand body are correlated within this frame, and four architecture hierarchies, composite channel, single channels, point bars and lateral accretion sandbody are identified, The result indicates that Minghuazhen Formation of Gangxi oilfield are dominated by meandering river deposition, including two types of channel sandbodies, narrow band and wide band channel sandbody, and each of them has different characteristics of facies variation laterally. Based on the identification of composite channel, according to the spatial combination patterns and identified signs of single channel, combined with channel sandbody distribution and tracer material data, single channel sandbodies are identified. According to empirical formula, point-bar scales of the study area are predicted, and three identification signs are summarized, that is, positive rhythm in depositional sequence, the maximum thick sand and near close to the abandoned channel, and point bars are identified. On the basis of point bar recognition, quantitative architecture models inner point bar are ascertained, taking the lateral accretion sand body and lateral accretion shale beddings in single well as foundation, and quantitative architecture models inner point bar as guidance, and result of tracer material data as controlling, the the lateral accretion sand body and lateral accretion shale beddings are forecasted interwell, so inner architecture of point bar is anatomied. 3-D structural model, 3-D facies model and 3-D petrophysical properties models are set up, spatial distribution characteristics of sedimentary facies and petrophysical properties is reappeared. On the basis of reservoir architecture analysis and performance production data, remaining oil distribution patterns controlled by different hierarchy architecture units, stacked channel, single channel and inner architecture of point bar, are summarized, which will help to guide the additional development of oil fields.

叠前叠后联合反演技术及其在QHD32-6油田的应用研究

What geophysical inversion studied includes the common mathematics physical property of inversion and the constitution and appraisal method of solution in geophysics domain, i.e. using observed physical phenomenon from the earth surface to infer space changing and physical property structure of medium within the earth. Seismic inversion is a branch of geophysical inversion. The basic purpose of seismic inversion is to utilizing seismic wave propagating law in the medium underground to infer stratum structure and space distribution of physical property according to data acquisition, processing and interpretation, and then offer the vital foundation for exploratory development. Poststack inversion is convenient and swift, its acoustic impedance inversion product can reflect reservoir interior changing rule to a certain degree, but poststack data lack abundant amplitude and travel time information included in prestack data because of multiple superimpose and weaken the sensitiveness which reflecting reservoir property. Compared with poststack seismic inversion, prestack seismic inversion has better fidelity and more adequate information. Prestack seismic inversion, including waveform inversion, not only suitable for thin strata physical property inversion, it can also inverse reservoir oil-bearing ability. Prestack seismic inversion and prestack elastic impedance inversion maintain avo information, sufficiently applying seismic gathering data with different incident angle, partial angle stack, gradient and intercept seismic data cube. Prestack inversion and poststack inversion technology were studied in this dissertation. A joint inversion method which synthesize prestack elastic wave waveform inversion, prestack elastic impedance inversion and poststack inversion was proposed by making fully use of prestack inversion multiple information and relatively fast and steady characteristic of poststack inversion. Using the proposed method to extract rock physics attribute cube with clear physical significance and reflecting reservoir characterization, such as P-wave and S-wave impedance, P-wave and S-wave velocity, velocity ratio, density, Poisson ratio and Lame’s constant. Regarding loose sand reservoir in lower member of Minghuazhen formation, 32-6 south districts in Qinhuangdao,as the research object, be aimed at the different between shallow layer loose sand and deep layer tight sand, first of all, acquire physical property parameters suitable for this kind of heavy oil pool according to experimental study, establishing initial pressure and shear wave relational model; Afterwards, performing prestack elastic wave forward and inversion research, summarizing rules under the guidance of theoretical research and numerical simulation, performing elastic impedance inversion, calculating rock physics attributes; Finally, predicting sand body distribution according to rock physics parameters, and predicting favorable oil area combine well-logging materials and made good results.

松辽盆地古龙地区葡萄花油层组沉积成岩相研究

The sediment and diagenesis process of reservoir are the key controlling factors for the formation and distribution of hydrocarbon reservoir. For quite a long time, most of the research on sediment-diagenesis facise is mainly focusing on qualitative analysis. With the further development on exploration of oil field, the qualitative analysis alone can’t meet the requirements of complicated requirements of oil and gas exploreation, so the quantitative analysis of sediment-diagenesis facise and related facies modling have become more and more important. On the basis of the research result from stratum and sediment on GuLong Area Putaohua Oil Layer Group, from the basic principles of sedimentology, and with the support from the research result from field core and mining research results, the thesis mainly makes the research on the sediment types, the space framework of sands and the evolution rules of diagenesis while mainly sticking to the research on sediment systement analysis and diagenetic deformation, and make further quantitative classification on sediment-diageneses facies qualitatively, discussed the new way to divide the sediment-diagenesis facies, and offer new basis for reservoir exploration by the research. Through using statistics theory including factor analysis, cluster analysis and discriminant analysis, the thesis devided sediment-diagenesis facies quantitatively. This research method is innovative on studying sediment-diagenesis facies. Firstly, the factor analysis could study the main mechanism of those correlative variables in geologic body, and then could draw a conclusion on the control factors of fluid and capability of reservoir in the layer of studying area. Secondly, with the selected main parameter for the cluster analysis, the classification of diagenesis is mainly based on the data analysis, thus the subjective judgement from the investigator could be eliminated, besides the results could be more quantitative, which is helpful to the correlative statistical analysis, so one could get further study on the quantitative relations of each sediment-diagenesis facies type. Finally, with the reliablities of discriminant analysis cluster results, and the adoption of discriminant probability to formulate the chart, the thesis could reflect chorisogram of sediment-diagenesis facies for planar analysis, which leads to a more dependable analytic results.According to the research, with the multi-statistics analysis methods combinations, we could get quantitative analysis on sediment-diagenesis facies of reservoir, and the final result could be more reliable and also have better operability.

鄂尔多斯盆地环县地区长6-长8沉积相及储层研究

The exploration in recent years shows that the Yanchang Formation in the southwest of Ordos Basin is of great resource potential and good exploration and exploitation prospect. In the thesis ，sedimentary source analysis，sedimentary system，sedimentary microfacies，sandstones distribution and reservoir characteristic are studied and favorable oil area are forecasted in Chang6-Chang8 of Yanchang formation in HuanXian region， by mainly study on the data of field section observation ，core observation, well logging explaination and routine microscope slice identification，scanning Electron Microscope and reservoir analysis of lithology and physical property , Under the guidance of such advanced theories and methods as sedimentology，reservoir sedimentology，lithological oil pool and so on. The stratum of Chang6-Chang8 of Yanchang formation could be divided into pieces of member following the principles that firstly contrasting the big segments, then contrasting the small segments, being controlled by cycle and consulting the thickness etc.And the characteristic of stratum are detailed discussed , respectively. Based on the source direction of the central basin, heavy and light minerals are used to analyse source direction of Chang6 and Chang8 member, in HuanXian area. Research result shows that the source of Chang6 and Chang8 member is mixed provenance,including west-south,west and east-north. By the study of rock types、 sedimentary conformation、lithology and electromotive curve combination and palaeo-biology,lake、delta and braided delta mianly developed in study area are recognized, Subaqueous distributary channels in delta front and in braided delta front, and sand body in deep-lake turbidite, are the main reservoir.forthermore,the characteristic of depositional system and sandy body in space are discussed. Applied with routine microscope slice identification, Scanning Electron Microscope, reservoir lithology and physical property analysis and other analytic machinery, Feldspar-lithic fine-sandstone and feldspar fine-sandstone are mainly sandstone of Y Chang6-Chang8 in Huanxian area, small pore and tiny pore are the main pore types, tiny throat type and micro-fine throat type are widely developed , secondary dissolution porosity, intercrystal porosity, tiny pore and micro-crack are main pore types.Intergranular porosity and dissolution porosity secondary is the main pore secondary. The dominant diagenesis types in the area are compaction, cementation, replacement and dissolution. Chlorite films cementation facies, carbonate cementation facies ,mud cementation compaction facie, compaction 、pressure solution facies are the main diagenetic facies,in which Chlorite films cementation facies is the best diagenetic facies in study area. Reservoir influence factor analysis ,rock types are the main factor forming this low-pore and low-permeability of Chang6-Chang8 member in study area,and relatively higher permeability area are cortrolled by sedimentary facies distribution, diagenesis improved reservoir physical property. According to the distributing of sedimentary micro-facies and sandy body , and the test oil, favorable region in Chang6-Chang8 are forecasted.

冀东油田柳南地区新近系油藏剩余油形成及分布规律研究

This thesis is based on the research project of Study on the Geological Characteristics and Remaining Oil Distribution Law of Neogene Reservoirs in Liunan Area, which is one of the key research projects set by PetroChina Jidong Oilfield Company in 2006. The determination of remaining oil distribution and its saturation changes are the most important research contents for the development and production modification of oilfields in high water-cut phases. Liunan oilfield, located in Tangshan of Hebei Province geographically and in Gaoliu structural belt of Nanpu sag in Bohai Bay Basin structurally, is one of the earliest fields put into production of Jidong oilfield. Focusing on the development problems encountered during the production of the field, this thesis establishes the fine geological reservoir model through the study of reservoir properties such as fine beds correlation, sedimentary facies, micro structures, micro reservoir architecture, flow units and fluid properties. Using routine method of reservoir engineering and technology of reservoir numerical modeling, remaining oil distribution in the target beds of Liunan area is predicted successfully, while the controling factors of remaining oil distribution are illustrated, and the model of remaining oil distribution for fault-block structure reservoirs is established. Using staged-subdivision reservoir correlation and FZI study, the Strata in Liunan Area is subdivided step by step; oil sand body data-list is recompiled; diagram databases are established; plane and section configuration of monolayer sandstone body, and combination pattern of sandstone bodys are summarized. The study of multi-level staged subdivision for sedimentary micro-facies shows that the Lower member of Minghuazhen formation and the whole Guantao formation in Liunan Area belong to meandering river and braided river sedimentary facies respectively, including 8 micro facies such as after point bar, channel bar, channel, natural levee, crevasse splay, abandoned channel, flood plain and flood basin. Fine 3D geological modeling is performed through the application of advanced software and integration of geological, seismic logging and reservoir engineering data. High resolution numerical simulation is performed with a reserve fitting error less than 3%, an average pressure fitting fluctuation range lower than 2Mpa and an accumulate water cut fitting error less than 5%. In this way, the distribution law of the target reservoir in the study area is basically recognized. Eight major remaining oil distribution models are established after analysis of production status and production features in different blocks and different layers. In addition, fuzzy mathematics method is used to the integreted evaluation and prediction of abundant remaining oil accumulation area in major production beds and key sedimentary time units of the shallow strata in Liunan Area and corresponding modification comments are put forward. In summary, the establishment of fine reservoir geological model, reservoir numerical simulation and distribution prediction of remaining oil make a sound foundation for further stimulation of oilfield development performance.

孤岛油田曲流河微相单砂体储层建筑结构及挖潜方向研究

In the intermediary and later stage of oil field development, remaining oil disperses fiercely, the contradiction in the layer has become the main problem and the distribution of remaining oil is transforming to the difference of single sand-body. So, the fine description research of reservoir is becoming a tendency and the methods of remaining oil research need new developments. In the research of “The Single-sand-body Architectural Element and Potentiality Analysis Research of Meandering River, GuDao Oil Field”, the research principle is analytical hierarchy process and schema prescription what are reservoir fine description methods under the condition of dense well pattern. The knowledge of regional sedimentary system and sedimentary facies is the foundation of this research. According to the 3D distribution model of the microfacies sand-body of fluvial facies, stratigraphic unit classification & coenocorrelation of 154 wells are completed in the research of meandering river sand-body in Ng3-4. In this research, the 3D distribution of microfacies sand-body in the main layers are settled. The architectural element model of Ng4 point bar is analysed using the drill core and FMI data. According to the overgrow model of point bar, the surfaces of lateral accretion is traced and the architectural element model of point bar is settled. In the research, the control of micro-facies sand-body of meandering river to the distribution of remaining oil is analysed and the potential area is proposed. All these will play an important role in the development of GuDao oil field. In this research, abundant of logging data, drill core data and production performance data are used to analyse the contributing factor of single sand-body in the Ng3-4 meandering river. Using the technology of geological modeling, all that are researched including the 3D distribution scales of meandering river point bar, the control affection of inner lateral accretion layer to the distribution of oil & gas and remaining. Then, the way of remaining oil development in the sand-body of meandering river is improved. The innovation of the research technology includes (1) the presentation of the conception and research methods of micro-facies sand-body (2) enriching the content of reservoir architectural element research and (3) to renew the research method of remaining oil analysis. The research has practiced with obvious effect.(1)It is deepened into understand the river facies reservoir construction of Gudao oil field, By Building the reservoir construction and studying the effect of diffent deposit or geological interface to fluid partition and to the distribution of the remaining oil, we improved the understanding to the distribution of the remaining oil；(2)By building the distribution mod of the remaining oil in the reservoir construction and making the remaining oil description detailed,the development direction of old oil field is more clear；(3)Expanded the application scales of the horizontal well and enhanced the application effects of the horizontal well technique , we designed and drilled 23 ports horizontal wells in all , the cumulative hydrocarbon production is 10.6*104 t；(4) According to the findings of the internal building structure in reservoir of the fluvial facies in the region of interest, and uniting the injection/production corresponding states、the producing history and the dynamic monitoring documents of the oil/water wells in the flooding units , we researched the residual oil distribution in the point bar , and found the distribution regular patterns of the remaining oil, and comprehended the distribution of the remaining oil . In base of that , we proceeded the optimizing designs of the oil well potentialities , and advanced the effect of the treatment potentials . It is proved that , it was very important that internal building structure research of the single sand body of reservoir for guiding the high efficiency potentialities of the remaining oil in the high water cut stage .

孤岛油田中二北区Ng3～6储层建筑结构与油水分布规律研究

The reservoir of Zhongerbei region in Gudao Oilfield is a typical fluvial facies deposit, its serious heterogeneity of the reservoir caused the distribution of remaining oil in mature reservoirs is characterized by highly scattered in the whole field, and result to declination of production, tap potential and stabilize production is more difficult. Reservoir modeling based on lay scale can not fulfill requirement. How to further studied reservoir heterogeneity within the unit and establish the finer reservoir modeling is a valid approach to oil developing. The architectural structure elements analysis is the effectively method to study reservoir heterogeneity. Utilize this method, divide the reservoirs of Gudao Oilfield into ten hierarchies. The priority studying is sixth, seven hierarchies, ie single sand layers sand bodies By the identification of sixth, seven hierarchies, subdivide the reservoir to the single genetic unit. And to subdivide by many correlation means, such as isometry and phase transition, accomplish closure and correlation of 453 wells.Connectting fluvial deposit pattern, deposition characteristic with its log, build the inverting relation between “sedimentary facies” and “electrofacies” The process emphasize genetic communication and collocation structure of genetic body in space. By detailed architecture analyses sandbodies’ structure, this paper recognize seven structure elements, such as major channel, abandoned channel, natural levee, valley flat, crevasse splay, crevasse channel and floodplain fine grain.Combination identification of architectural structure elements with facieology and study of deposition characteristic, can further knowing genesis and development of abandoned channel. It boost the accuracy to separation in blanket channel bodies distribution, and provide reference to retrieving single channel boundary. Finally, establish fine plane and section construction. On basis architectural structure map, barrier beds and interbeds isopach map and mini-structure map, considering single thin layers to be construction unit, the main layer planimetric maps have drawn and the inner oil-water boundary have revealed. All account that architectural structure elements control remaining oil distribution in layer, and develop the study on architectural structure elements to direct horizontal well is succesful.