78 resultados para Munc18-1a


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Using the large acceptance apparatus FOPI, we study central collisions in the reactions (energies in A GeV are given in parentheses): Ca-40 + Ca-40 (0.4, 0.6, 0.8, 1.0, 1.5, 1.93), Ni-58 + Ni-58 (0.15, 0.25, 0.4), Ru-96+Ru-96 (0.4, 1.0. 1.5), (96)zr+(96)zr 1.0, 1.5), Xe-129+CsI (0.15, 0.25, 0.4), Au-197 + Au-197 (0.09, 0.12, 0.15, 0.25, 0.4, 0.6, 0.8, 1.0, 1.2, 1.5). The observables include cluster multiplicities, longitudinal and transverse rapidity distributions and stopping, and radial flow. The data are compared to earlier data where possible and to transport model simulations. (C) 2010 Elsevier B.V. All rights reserved.

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Within the framework of the improved isospin-dependent quantum molecular dynamics model, the dynamics of pion emission in heavy-ion collisions in the region of 1A GeV energies as a probe of nuclear symmetry energy at suprasaturation densities is investigated systematically. The total pion multiplicities and the pi(-)/pi(+) yields are calculated for selected Skyrme parameters SkP, SLy6, Ska, and SIII and also for the cases of different stiffness of symmetry energy with the parameter SLy6. The influence of Coulomb potential, symmetry energy, and in-medium pion potential on the pion production is investigated and compared to each other by analyzing the distributions of transverse momentum and longitudinal rapidity and also the excitation functions of the total pion and the pi(-)/pi(+) ratio. The directed flow, elliptic flow, and polar-angle distributions are calculated for the cases of different collision centralities and also the various stiffnesses of the symmetry energies. A comparison of the calculations with the available experimental data is performed.

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以故障实例详细地叙述了岛津VD-1A型X射线衍射仪电流稳定器系统的故障分析及排除方法。

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本文以故障实例详细介绍了岛津VD-IA型x社线1行射议信号检测系统一种典型故障的分析及排除方法。

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介绍了岛津VD_1A型X射线衍射仪冷却循环水系统的故障分析及排除方法。

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以实例详细地介绍了岛津VD1A型X射线衍射仪记录系统的故障分析及排除方法。

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The damage mechanisms and micromachining of 6H SiC are studied by using femtosecond laser pulses at wavelengths between near infrared (NIR) and near ultraviolet (NUV) delivered from an optical parametric amplifier (OPA). Our experimental results indicate that high quality microstructures can be fabricated in SiC crystals. On the basis of the dependence of the ablated area and the laser pulse energy, the threshold fluence of SiC is found to increase with the incident laser wavelength in the visible region, while it remains almost constant for the NIR laser. For the NIR laser pulses, both photoionization and impact ionization play important roles in electronic excitation, while for visible lasers, photoionization plays a more important role.

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 利用RNA减法杂交、差异筛选和5’-RACE等方法从水稻分离到了一花药绒毡层特异表达的基因RA39。Southern 杂交表明,RA39在水稻基因组中是以单拷贝的形式存在的。RT-PCR 结果初步表明,RA39是一水稻花药特异表达的基因。RNA原位杂交进一步表明,RA39主要在水稻花药的绒毡层中表达,而且在小孢子母细胞减数分裂期和四分体时期表达量最高。RA39 cDNA全长1013bp,编码298个氨基酸残基。 RA39 cDNA与数据库中的已知序列没有明显的相似性,由其推测的多肽与核糖体失活蛋白(ribosome-inactivating protein, RIP)的序列相似在19-34%之间。多重序列排列分析结果表明构成RIPs活性位点的5个关键氨基酸残基在RA39中是保守的,在蓖麻毒蛋白中分别为Tyr80、 Tyr123、 Glu177、 Arg180 and Trp211 。利用原核表达系统,通过蛋白质分离和纯化获得了在SDS电泳图谱上为单一条带的纯的RA39蛋白,用兔rRNA作底物进行的酶活性分析证明该蛋白有N-糖基化作用,是一种类型I的核糖体失活蛋白。反义转基因植株的花粉用TTC进行活性染色结果显示其活性明显减弱,成熟的T0代反义转基因植株的结实率明显降低,只有对照的20-60%。这说明,RA39蛋白可能和小孢子母细胞的发育相关。   酵母DMC1是减数分裂过程中同源染色体配对和重组修复所必需的减数分裂特异基因。根据酵母Dmc1和拟南芥AtDmc1的保守区设计简并性引物,通过RT-PCR和RACE等方法,从水稻中分离出了酵母DMC1的同源基因OsDMC1。RT-PCR分析表明,OsDMC1在花中表达量最高,在根中表达量较低,在叶片和幼芽几乎不表达。水稻基因组中有两个拷贝的OsDMC1。OsDmc1蛋白与酵母Dmc1和拟南芥AtDmc1氨基酸一致性分别为53%和81%。   酵母Spo11在减数分裂过程中具有催化DNA双链断裂从而起始同源重组的功能。以酵母Spo11氨基酸序列为探针和现有的数据库通过数据分析,结合RACE技术,克隆了水稻SPO11同源基因OsSPO11-1, OsSPO11-1是一个单拷贝基因,有3个外显子和2个内含子,在转录过程中通过内含子的可变剪切产生4个不同的转录本(OsSPO11-1A、OsSPO11-1B、OsSPO11-1C和OsSPO11-1),其中,OsSPO11-1A是一个未剪切的转录本,OsSPO11-1B包含内含子2,OsSPO11-1C包含内含子1,OsSPO11-1D是一个完全剪切的转录本。这些转录本编码的蛋白有一致的246氨基酸残基的C-端,包含了Spo11/TopVIA家族蛋白共有的5个功能基元,是该家族的新成员。OsSPO11-1A和 OsSPO11-1C在花中优势积累,OsSPO11-1B是花特异的,而OsSPO11-1D在营养器官中优势积累。在花中该基因主要在减数分裂的花粉母细胞和胚曩中表达,在减数分裂期的绒毡层细胞和不同花器官的微管束细胞中也表达。这些结果说明内含子涉及到了OsSPO11-1表达的器官特异性调节,该基因除了参与减数分裂的调节外,在体细胞的发育中可能起重要作用。

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本文对神农架地区广泛分布的米心水青冈林和锐齿槲栎林的种群和群落学特征、干扰历史、更新策略、生物量、生产量及元素循环特征进行了研究。得到如下结论: 1 米心水青冈林是神农架地区山地垂直分布的地带性植被类型,主要群落学特征为:(1)建群种明显,该区域主要有2种类型,即米心水青冈林和米心水青冈、锐齿槲栎林;(2)群落结构简单,但物种组成丰富,在6600m~2样地中出现高等植物(不含苔藓植物)77科150属271种,组成种类以蔷薇科、百合科、忍冬科、虎耳草科、樟科、杜鹃花科和壳斗科为主;(3)群落乔木层(占重要值的12%)和灌木层(占盖度的15%)中含有一定比例的常绿树种;(4)群落生活型以高位芽植物(70.89%)占绝对优势,其次为地面芽植物(15.50%)和地下芽植物(12.92%)。 2 米心水青冈是多主干的树种,萌枝现象普遍,但萌枝数量不同地点差异较大。通过萌枝产生的枝群体平均密度为257 ± 99.3n•hm~(-2)。枝群体的年龄结构表现为“幼龄个体数目较多型”和“中国年龄阶段数目较多型”,并且有较多的枝群体表现出一致的年龄结构。从整个群落米心水青冈的年龄结构来看,表现出发展型种群的特点。枝群体的分布格局为随机分布。9丛米心水青冈完整的年轮分析结果表明,它们萌枝的时间不是边疆的,而与森林的受干扰有关。根据83个圆盘和生长锥芯资料,米心水青冈在萌枝后成长为乔木层或林冠层的过程中,径向生长表现为5种模式。这是丛株内竞争的结果。萌枝在米心水青冈林的维持和发展过程中,具有重要的生态学作用。 3 锐齿槲栎林是神农架地区山地垂地分布的地带性植被类型,主要群落学特征为:(1)建群种明显,该区域主要有2种类型,即锐齿槲栎林和锐齿槲栎、米心水青冈林;(2)群落乔木层和灌木层中含有一定比例的常绿树种,和暖温带的落叶栎林有较大差异;(3)群落物种组成丰富,不仅具有典型的温带科属,还有典型亚热带分布的科属,组成种类主要以蔷薇科、百合科、忍冬科、虎耳草科、山茱萸科、杜鹃花科、壳斗科和樟科;(4)生活型以高位芽植物(66.32%)占绝对优势,其次为地面芽(23.51%)和地下芽(9.47%)植物。 4 通过样地调查、树干解析及直径分析法,对米心水青冈林和锐齿槲栎林受压和释压历史及更新策略进行了研究。米心水青冈直径生长表现为5种模式。而锐齿槲栎只表现为2种模式。85.9 ± 6.9%的米心水青冈有过受压过程,平均受压2.1 ± 0.8次,平均受压时间为47 ± 24.1a,最长受压时间73a,平均释压次数为1.6 ± 0.7次,平均释压时间为23 ± 21.5a,而60.83%的锐齿槲栎都均有1次受压。平均受压时间为19 ± 14a,受压后没有表现出释压过程。结合高生长和径向生长,认为米心水青冈是耐阴树种,它的更新策略是在林下形成苗性萌枝,在有林窗形成时释压生长进入乔木层;而锐齿槲栎是不耐阴树种,它的更新策略是通过产生大量种子,当有大的林窗时,幼苗在林窗内生长逐步进入乔木层。 5 神农架地区102-130a成熟米心水青冈林的生物量在251.31-358.63T•hm~(-2)之间,平均为288.70 ± 48.30T•~(-2),20-60a锐齿槲栎林群落生物量在134.85-301.20T•hm~(-2),平均为231.60 ± 78.10T•hm~(-2)。虽然米心水青冈林和锐齿槲栎林灌木层草本层及藤本植物组成很丰富,但二种类型森林生物量的95%以上集中在乔木层。乔木层生物量主要集中在少数优势种中。在米心水青冈林生物量从大到小的序列中,前5种植物分别占乔木层总生物量58.67%-96.37%不等,同样锐齿槲栎林前5种植物占群落生物量的68.13%-95.26%。常绿植物占乔木层生物量的比例变化较大,米心水青冈林中占2.85-18.70%,锐齿槲栎林中一般常绿植物占0.8-9.98%,只有1个锐齿槲栎林样地常绿植物(主要是粉白杜鹃)占乔木层生物量的44.04%。米心水青冈林生物量根冠比为0.27 ± 0.05,锐齿槲栎林为0.21 ± 0.06。神农架地区米心水青冈林的生物量,在成熟的欧洲水青冈林及日本的水青冈林生物量范围之内,而锐齿槲栎林生物量远远大于我国温带落叶栎林的生物量。 6 神农架地区102-130a米心水青冈林生产量范围在1857-2786g•m~(-2)•a~(-1),平均为2330 ± 397 g•m~(-2)•a~(-1)。20-60a锐齿槲栎林的生产量范围在1319-2521 g•m~(-2)•a~(-1),平均为1930 ± 498 g•m~(-2)•a~(-1)。米心水青冈林和锐齿槲栎林乔木层生产量占群落总生产量的95%以上,乔木层各器官生产量大小顺序为叶> 树干> 枝和根,其中叶生产量占乔木层的一半以上,达53.87 ± 2.72%(米心水青冈林)和57.31 ± 6.23%(锐齿槲栎林)。在乔木层生产量从大到小的序列中,前5种植物平均占乔木层总生产量的81.03 ± 13.94%(米心水青冈林,范围在62.75%-92.66%)和84.23 ± 9.68%(锐齿槲栎林,范围在68.54-95.11%)。米心水青冈林和锐齿槲栎林群落地下部分生产量占总生产量的比例分别为11.29 ± 1.02%和9.22 ± 2.72。和我国其它地区地带性植被类型相比。米心水青冈林和锐齿槲栎生产量是较高的,和亚热带绿阔叶林生产量接近,但在器官分配上两者差异较大。 7 米心水青冈林和锐齿槲栎林土壤均呈酸性。其中锐齿槲栎林地土壤酸性更强。土壤元素特征表现为Al>C>K>Mg>Ca>N>S、P的特点,富铝化作用明显。8种元素在群落优势植物不同部位含量差异较大,N、P、K、Ca、Mg基本上是以叶片含量最高,树干或根中最低。仅从叶片来看,元素特征表现为C>Ca、N>K>Mg>S>P、Al。优势植物的C/N和C/P显著高于暖温带落叶阔叶林优势植物。8种元素在米心水青冈林和锐齿槲栎林中积累量分别为147.09 ± 25.60和116.00 ± 37.63 Mg hm~(-2)a~(-1),其中97%以上积累在群落乔木层。两种森林类型各元素的积累量都表现为C>Ca, N> K> Mg> P> S> Al的特点。米心水青风林和锐齿槲栎林8种元素的年存留量分别为6263 ± 90.8和5946 ±246 kg hm~(-2)a~(-1),其中N、P、K、Ca、Mg 5种主要营养元素的存留量分别为179.7 ± 18.2和169.4 ± 23.5kg hm~(-2) a~(-1)。两种森林类型各元素的存留量都表现为C> N> Ca> K> Mg> S> P> Al。

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检测了滇池水饲养 1a的椭圆背角无齿蚌 (Anodontawoodianaelliptica)体内重金属等元素含量 ,结果显示椭圆背角无齿蚌体内锌含量为 7.0 8× 1 0 2 mg/kg ,铅为 3 5 .6 8mg/kg ,铜为 9.0 4mg/kg ,铁为 4 .74× 1 0 3mg/kg ,砷为 6 2 .0 9mg/kg ,磷为 4 .4 4× 1 0 4mg/kg .与滇池水中锌 0 .0 2 0 5 μg/L ,铅 0 .4 1 2 μg/L ,铜 3 .1 98μg/L相比较 ,表明椭

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对耳石边缘年轮形成周期进行了分析。结果表明 ,鲫耳石上 1a形成 1个年轮 ,年轮形成时期洪湖种群主要在 4~ 6月 ,洞庭湖种群主要在 3~ 5月。星耳石和微耳石一样 ,可用于鲫年龄鉴定 ,而且不经磨片即可直接读取年轮 ,完整星耳石和微耳石与微耳石磨片上的年轮读数的吻合率均在 80 %以上 ,误差不超过 1龄。但相对而言 ,微耳石上年轮更易观察。文中对耳石形态和年轮特征作了描述

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<正> 葡萄藻作为一种新的再生性能源的研究,已引起了不少学者的重视。其中较为关键的是对葡萄藻所含脂类物质的研究。过去通常采用重量法及各类色谱法测定植物中的脂类物质。这些方法不但繁琐,而且均需一个较长时间的提取过程。近年来国外采用一种新型染料——尼罗

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A 186-bp sequence with imperfect terminal inverted repeats and target direct repeats but without any transposase-encoding capacity was found to be transposable in an isolate derived from Microcystis sp. FACHB 854. This miniature insertion element, designated as ISM854-1, and with its homologues present at least 10 copies in the genome of Microcystis FACHB 854, is inserted into the 8-bp long and AT-rich target sequences, but none or few in other Microcystis strains. A variant of ISM854-1, denoted ISM854-1A, has perfect inverted repeat sequences and may transpose in pairs in a structure like a composite transposon. This is the first report of non-autonomous transposition of a mini-IS in a cyanobacterium.

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Photochromic diarylethene, 1,2-bis[2-methyl-5-(3-fluorophenyl)-3-thienyl] perfluorocyleopentene (1a), was synthesized. The compound showed good photochromic reactions both in solution and in PMMA matrix by photo-irradiation. Using the diarylethene lb/PMMA film as recording medium and a He-Ne laser for recording and readout, four types of polarization holographic optical recording were accomplished for the first time. The results show that the orthogonal circular polarization recording is the best method for holographic optical recording when the target photochromic diarylethene is used as recording material. (c) 2006 Published by Elsevier B.V.

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A new unsymmetrical photochromic diarylethene 1a is synthesized, and the photochromic properties of it are also investigated. The compound exhibits good photochromism with UV/ visible light irradiation. Compound 1a in polymethyl methacrylate ( PMMA ) film changes color upon 313- nm light irradiation from colorless to blue, in which the absorption maximum is observed at 587 nm. Photon- mode polarization multiplexing holographic optical recording is performed successfully using this compound as a recording medium. In the diarylethene 1b/ PMMA film, polarization multiplexing hologram recording and retrieval, and a combination with the angular multiplexing scheme, are demonstrated systematically. The results indicate that recording capacity can be significantly improved with the combined method of polarization and angular multiplexing holographic recording. (C) 2008 Society of Photo- Optical Instrumentation Engineers.